BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38782 (295 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3RJ1-G nrPDB 608 e-175 4H61-A nrPDB 102 1e-22 5U0S-F nrPDB 102 2e-22 2D3T-A nrPDB 30 1.4 1XVY-A nrPDB 29 2.1 3UE3-A nrPDB 28 4.7 >3RJ1-G nrPDB Length = 295 Score = 608 bits (1568), Expect = e-175, Method: Compositional matrix adjust. Identities = 295/295 (100%), Positives = 295/295 (100%) Query: 1 MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNA 60 MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNA Sbjct: 1 MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNA 60 Query: 61 AVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSV 120 AVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSV Sbjct: 61 AVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSV 120 Query: 121 REPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYH 180 REPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYH Sbjct: 121 REPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYH 180 Query: 181 LNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNGNNAGGGSNKSSVRPTGGANMAT 240 LNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNGNNAGGGSNKSSVRPTGGANMAT Sbjct: 181 LNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNGNNAGGGSNKSSVRPTGGANMAT 240 Query: 241 VPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGITTEMLDKLMVTSIRSTPNYI 295 VPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGITTEMLDKLMVTSIRSTPNYI Sbjct: 241 VPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGITTEMLDKLMVTSIRSTPNYI 295 >4H61-A nrPDB Length = 175 Score = 102 bits (255), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 63/214 (29%), Positives = 99/214 (46%), Gaps = 53/214 (24%) Query: 6 LDELQWKSPEWIQVFG-LRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNAAVNM 64 L +QW+ PEW+Q G LRTENVL+YF++SPF+ SNN+++KMQ QF Sbjct: 6 LTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQF------------ 53 Query: 65 TQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSVREPD 124 N + L D L +L +L G ++V+ R P Sbjct: 54 --NALDLGD-----------------------------LNSQLKRLTGIQFVIIHERPPF 82 Query: 125 FWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNST 184 WVI+KQ R N + E+ PL Y++ NIY +P + ++ +R+++ +Y Sbjct: 83 LWVIQKQNRLNEN--------EVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKA 134 Query: 185 LESLYDLIEFQPSQGVHY-KVPTDTSTTATAATN 217 L + ++ P +G Y K+ D + TN Sbjct: 135 LTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTN 168 >5U0S-F nrPDB Length = 216 Score = 102 bits (255), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 63/214 (29%), Positives = 99/214 (46%), Gaps = 53/214 (24%) Query: 6 LDELQWKSPEWIQVFG-LRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNAAVNM 64 L +QW+ PEW+Q G LRTENVL+YF++SPF+ SNN+++KMQ QF Sbjct: 11 LTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQF------------ 58 Query: 65 TQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSVREPD 124 N + L D L +L +L G ++V+ R P Sbjct: 59 --NALDLGD-----------------------------LNSQLKRLTGIQFVIIHERPPF 87 Query: 125 FWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNST 184 WVI+KQ R N + E+ PL Y++ NIY +P + ++ +R+++ +Y Sbjct: 88 LWVIQKQNRLNEN--------EVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKA 139 Query: 185 LESLYDLIEFQPSQGVHY-KVPTDTSTTATAATN 217 L + ++ P +G Y K+ D + TN Sbjct: 140 LTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTN 173 >2D3T-A nrPDB Length = 715 Score = 29.6 bits (65), Expect = 1.4, Method: Compositional matrix adjust. Identities = 24/85 (28%), Positives = 35/85 (41%), Gaps = 17/85 (20%) Query: 140 GSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNSTLESLYDLIEFQPSQG 199 S KG + +D +I+GA+I + FK+ + L++ + N DL Sbjct: 51 ASVKGVIVSSGKDVFIVGADITEFVENFKLPDAELIAGNLEANKIFSDFEDL-------- 102 Query: 200 VHYKVPTDTSTTATAATNGNNAGGG 224 VPT AA NG GGG Sbjct: 103 ---NVPT------VAAINGIALGGG 118 >1XVY-A nrPDB Length = 309 Score = 29.3 bits (64), Expect = 2.1, Method: Compositional matrix adjust. Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 9/36 (25%) Query: 126 WVIRKQ----RRTNNS-----GVGSAKGPEIIPLQD 152 W+ KQ RTNN+ GVG+A P+++PL+D Sbjct: 248 WITGKQGQEILRTNNAFEYAVGVGAASNPKLVPLKD 283 >3UE3-A nrPDB Length = 554 Score = 28.1 bits (61), Expect = 4.7, Method: Compositional matrix adjust. Identities = 27/86 (31%), Positives = 37/86 (43%), Gaps = 9/86 (10%) Query: 209 STTATAATNGNNAGGGSNKSSVRPTGGANMATVPSTTNVNMTVNTMGTGGQTIDNGTGRT 268 + T+ + N N+ GG SNK ++R G +M P +T TV GQ N T Sbjct: 247 AMTSWPSYNPNDKGGLSNKDAMRNRGAIDMFE-PGSTMKPFTVAAALESGQYTPNSIVNT 305 Query: 269 GNGNMGITTEMLDKLMVTSIRSTPNY 294 G M +L +IR T NY Sbjct: 306 APGTM--------RLGWHTIRDTHNY 323 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3RJ1-G nrPDB 511 e-145 5U0S-F nrPDB 249 1e-66 4H61-A nrPDB 248 2e-66 Sequences not found previously or not previously below threshold: 3UE3-A nrPDB 34 0.057 1VM8-A nrPDB 29 2.1 3GTD-A nrPDB 28 5.0 CONVERGED! >3RJ1-G nrPDB Length = 295 Score = 511 bits (1316), Expect = e-145, Method: Composition-based stats. Identities = 295/295 (100%), Positives = 295/295 (100%) Query: 1 MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNA 60 MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNA Sbjct: 1 MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNA 60 Query: 61 AVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSV 120 AVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSV Sbjct: 61 AVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSV 120 Query: 121 REPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYH 180 REPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYH Sbjct: 121 REPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYH 180 Query: 181 LNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNGNNAGGGSNKSSVRPTGGANMAT 240 LNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNGNNAGGGSNKSSVRPTGGANMAT Sbjct: 181 LNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNGNNAGGGSNKSSVRPTGGANMAT 240 Query: 241 VPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGITTEMLDKLMVTSIRSTPNYI 295 VPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGITTEMLDKLMVTSIRSTPNYI Sbjct: 241 VPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGITTEMLDKLMVTSIRSTPNYI 295 >5U0S-F nrPDB Length = 216 Score = 249 bits (635), Expect = 1e-66, Method: Composition-based stats. Identities = 63/214 (29%), Positives = 99/214 (46%), Gaps = 53/214 (24%) Query: 6 LDELQWKSPEWIQVFG-LRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNAAVNM 64 L +QW+ PEW+Q G LRTENVL+YF++SPF+ SNN+++KMQ QF Sbjct: 11 LTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQF------------ 58 Query: 65 TQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSVREPD 124 N + L D L +L +L G ++V+ R P Sbjct: 59 --NALDLGD-----------------------------LNSQLKRLTGIQFVIIHERPPF 87 Query: 125 FWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNST 184 WVI+KQ R N + E+ PL Y++ NIY +P + ++ +R+++ +Y Sbjct: 88 LWVIQKQNRLNEN--------EVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKA 139 Query: 185 LESLYDLIEFQPSQGVHY-KVPTDTSTTATAATN 217 L + ++ P +G Y K+ D + TN Sbjct: 140 LTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTN 173 >4H61-A nrPDB Length = 175 Score = 248 bits (633), Expect = 2e-66, Method: Composition-based stats. Identities = 63/214 (29%), Positives = 99/214 (46%), Gaps = 53/214 (24%) Query: 6 LDELQWKSPEWIQVFG-LRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNAAVNM 64 L +QW+ PEW+Q G LRTENVL+YF++SPF+ SNN+++KMQ QF Sbjct: 6 LTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQF------------ 53 Query: 65 TQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSVREPD 124 N + L D L +L +L G ++V+ R P Sbjct: 54 --NALDLGD-----------------------------LNSQLKRLTGIQFVIIHERPPF 82 Query: 125 FWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNST 184 WVI+KQ R N + E+ PL Y++ NIY +P + ++ +R+++ +Y Sbjct: 83 LWVIQKQNRLNEN--------EVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKA 134 Query: 185 LESLYDLIEFQPSQGVHY-KVPTDTSTTATAATN 217 L + ++ P +G Y K+ D + TN Sbjct: 135 LTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTN 168 >3UE3-A nrPDB Length = 554 Score = 34.5 bits (77), Expect = 0.057, Method: Composition-based stats. Identities = 26/78 (33%), Positives = 33/78 (42%), Gaps = 9/78 (11%) Query: 217 NGNNAGGGSNKSSVRPTGGANMATVPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGIT 276 N N+ GG SNK ++R G +M P +T TV GQ N T G M Sbjct: 255 NPNDKGGLSNKDAMRNRGAIDMFE-PGSTMKPFTVAAALESGQYTPNSIVNTAPGTM--- 310 Query: 277 TEMLDKLMVTSIRSTPNY 294 +L +IR T NY Sbjct: 311 -----RLGWHTIRDTHNY 323 >1VM8-A nrPDB Length = 534 Score = 29.1 bits (63), Expect = 2.1, Method: Composition-based stats. Identities = 15/71 (21%), Positives = 27/71 (38%) Query: 14 PEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNAAVNMTQNIMTLPD 73 P +I G E+ ++F + FF N V Q ++ + +N +++ Sbjct: 173 PWYIMTSGRTMESTKEFFTKHKFFGLKKENVVFFQQGMLPAMSFDGKIILEEKNKVSMAP 232 Query: 74 GKNGNLEEEFA 84 NG L A Sbjct: 233 DGNGGLYRALA 243 >3GTD-A nrPDB Length = 482 Score = 27.9 bits (60), Expect = 5.0, Method: Composition-based stats. Identities = 9/40 (22%), Positives = 19/40 (47%) Query: 109 KLDGTEYVLSSVREPDFWVIRKQRRTNNSGVGSAKGPEII 148 +++ + + E +W + QR NN + K P+I+ Sbjct: 26 RIESDSFGEIQIEEKFYWGAQTQRSLNNFKISKQKMPKIL 65 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.307 0.117 0.324 Lambda K H 0.267 0.0361 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,331,025 Number of Sequences: 36641 Number of extensions: 448661 Number of successful extensions: 991 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 964 Number of HSP's gapped (non-prelim): 34 length of query: 295 length of database: 10,336,785 effective HSP length: 96 effective length of query: 199 effective length of database: 6,819,249 effective search space: 1357030551 effective search space used: 1357030551 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 58 (27.1 bits)