BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38632 (267 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3HTK-C nrPDB 555 e-158 2YU4-A nrPDB 39 0.003 2QKK-A nrPDB 31 0.66 5OLM-A nrPDB 29 2.6 5OC1-A nrPDB 28 3.6 6AH3-B nrPDB 28 3.8 4S3O-B nrPDB 27 6.6 5Z56-q nrPDB 27 7.4 2HII-A nrPDB 27 7.6 2CKL-B nrPDB 27 7.7 5V8F-F nrPDB 27 7.8 5KDR-A nrPDB 27 7.8 >3HTK-C nrPDB Length = 267 Score = 555 bits (1429), Expect = e-158, Method: Compositional matrix adjust. Identities = 267/267 (100%), Positives = 267/267 (100%) Query: 1 MALNDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQIDETINQLVDSTSPSTIGIE 60 MALNDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQIDETINQLVDSTSPSTIGIE Sbjct: 1 MALNDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQIDETINQLVDSTSPSTIGIE 60 Query: 61 EQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTAP 120 EQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTAP Sbjct: 61 EQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTAP 120 Query: 121 KLSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPADEDDLQIEGGKI 180 KLSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPADEDDLQIEGGKI Sbjct: 121 KLSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPADEDDLQIEGGKI 180 Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPI 240 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPI Sbjct: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPI 240 Query: 241 MELRCKIAKMKESQEQDKRSSQAIDVL 267 MELRCKIAKMKESQEQDKRSSQAIDVL Sbjct: 241 MELRCKIAKMKESQEQDKRSSQAIDVL 267 >2YU4-A nrPDB Length = 94 Score = 38.5 bits (88), Expect = 0.003, Method: Compositional matrix adjust. Identities = 19/69 (27%), Positives = 32/69 (46%), Gaps = 5/69 (7%) Query: 178 GKIELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRD----CPQAACSQV-VSM 232 G TCPIT + + P+ ++ C H ++ D I ++ R CPQ CS + Sbjct: 4 GSSGFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRK 63 Query: 233 RDFVRDPIM 241 D ++D + Sbjct: 64 SDLIQDEAL 72 >2QKK-A nrPDB Length = 154 Score = 30.8 bits (68), Expect = 0.66, Method: Compositional matrix adjust. Identities = 12/36 (33%), Positives = 21/36 (58%) Query: 201 NHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFV 236 N +F +GI N++QG+ +A +V++ DFV Sbjct: 78 NSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFV 113 >5OLM-A nrPDB Length = 132 Score = 28.9 bits (63), Expect = 2.6, Method: Compositional matrix adjust. Identities = 22/83 (26%), Positives = 40/83 (48%), Gaps = 6/83 (7%) Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPI 240 E+TCPI P+ P +S +C H F ++ I +G + CP C Q +++ P Sbjct: 16 EVTCPICLDPFVEP-VSIECGHSFCQECISQVGKGGGS-VCP--VCRQRFLLKNL--RPN 69 Query: 241 MELRCKIAKMKESQEQDKRSSQA 263 +L + +KE ++ + +Q Sbjct: 70 RQLANMVNNLKEISQEAREGTQG 92 >5OC1-A nrPDB Length = 565 Score = 28.5 bits (62), Expect = 3.6, Method: Compositional matrix adjust. Identities = 33/116 (28%), Positives = 48/116 (41%), Gaps = 20/116 (17%) Query: 94 FKQSSDACPQIDLSTWDKYRTGELTAPKLSEL-YLNMPT--------PEPATMVNNTDTL 144 F+ SS+ +DL W RTG LTA + L +L +P+ P+PA N+ Sbjct: 331 FRDSSEF--NVDLDQWTNTRTGPLTALIANHLAWLRLPSNSSIFQTFPDPAAGPNSAHWE 388 Query: 145 KILKVLPYIWNDPTCVIPDLQNPADEDDLQIE---GGKIELTCPITCKPYEAPLIS 197 I W P PD + + I G I+L T P++ PLI+ Sbjct: 389 TIFS---NQWFHPAIPRPDTGSFMSVTNALISPVARGDIKLA---TSNPFDKPLIN 438 >6AH3-B nrPDB Length = 875 Score = 28.1 bits (61), Expect = 3.8, Method: Compositional matrix adjust. Identities = 25/72 (34%), Positives = 31/72 (43%), Gaps = 9/72 (12%) Query: 85 EHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTAPKLSELYLNMPTPEPATMVNNTDTL 144 + IK LK+ K+SS A P I L R G ++EL P P V T Sbjct: 183 QKIKTLKRMIKESSTANPNIKLLN---NRMGSYDCTGVNEL-----APIPKGRVKYTKRQ 234 Query: 145 KILKVLP-YIWN 155 K LP +IWN Sbjct: 235 KHFAWLPTHIWN 246 >4S3O-B nrPDB Length = 118 Score = 27.3 bits (59), Expect = 6.6, Method: Compositional matrix adjust. Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDP 239 EL CPI + + +++C H F D I L+ ++CP ++VS R DP Sbjct: 50 ELMCPICLDMLKNTMTTKECLHRFCADCIITALRS-GNKECPTCR-KKLVSKRSLRPDP 106 >5Z56-q nrPDB Length = 504 Score = 27.3 bits (59), Expect = 7.4, Method: Compositional matrix adjust. Identities = 12/34 (35%), Positives = 20/34 (58%) Query: 180 IELTCPITCKPYEAPLISRKCNHVFDRDGIQNYL 213 + L C I+ + E P +S NHV++R I+ Y+ Sbjct: 1 MSLICSISNEVPEHPCVSPVSNHVYERRLIEKYI 34 >2HII-A nrPDB Length = 257 Score = 27.3 bits (59), Expect = 7.6, Method: Compositional matrix adjust. Identities = 30/120 (25%), Positives = 54/120 (45%), Gaps = 23/120 (19%) Query: 65 DITSTYKLLSTYESESNSF-----DEHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTA 119 D++S K+LS S+ + D +K + ++ K + + I +K + +LT Sbjct: 71 DVSSVKKILSKASSKKATIELTETDSGLKIIIRDEKSGAKSTIYIK---AEKGQVEQLTE 127 Query: 120 PKLSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPADEDDLQIEGGK 179 PK++ L +N T E VL I D T V +++ +ED ++IE G+ Sbjct: 128 PKVN-LAVNFTTDES--------------VLNVIAADVTLVGEEMRISTEEDKIKIEAGE 172 >2CKL-B nrPDB Length = 165 Score = 27.3 bits (59), Expect = 7.7, Method: Compositional matrix adjust. Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDP 239 EL CPI + + +++C H F D I L+ ++CP ++VS R DP Sbjct: 54 ELMCPICLDMLKNTMTTKECLHRFCADCIITALRS-GNKECPTCR-KKLVSKRSLRPDP 110 >5V8F-F nrPDB Length = 435 Score = 27.3 bits (59), Expect = 7.8, Method: Compositional matrix adjust. Identities = 11/22 (50%), Positives = 14/22 (63%) Query: 11 SVPLHPKSGKYFHNLHARDLSN 32 S+PL PK K+ NL + LSN Sbjct: 83 SIPLEPKKAKHLMNLFRQSLSN 104 >5KDR-A nrPDB Length = 327 Score = 27.3 bits (59), Expect = 7.8, Method: Compositional matrix adjust. Identities = 17/38 (44%), Positives = 25/38 (65%), Gaps = 2/38 (5%) Query: 231 SMRDFVRDPIMELRCKIAKMKESQEQ-DKRSSQAIDVL 267 SM DF + P+ E+R KI +KESQ++ D + ID+L Sbjct: 13 SMLDFEK-PLFEIRNKIESLKESQDKNDVDLQEEIDML 49 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3HTK-C nrPDB 568 e-162 Sequences not found previously or not previously below threshold: 2YU4-A nrPDB 41 4e-04 5OLM-A nrPDB 34 0.11 5OC1-A nrPDB 31 0.49 5KDR-A nrPDB 30 0.93 4R8P-L nrPDB 30 1.1 2CKL-B nrPDB 30 1.1 4BY6-A nrPDB 30 1.2 4S3O-B nrPDB 30 1.2 2QKK-A nrPDB 29 2.3 5Z56-q nrPDB 29 3.3 4CEM-A nrPDB 28 5.0 4ARE-A nrPDB 27 7.6 2Y6I-A nrPDB 27 7.6 >3HTK-C nrPDB Length = 267 Score = 568 bits (1463), Expect = e-162, Method: Composition-based stats. Identities = 267/267 (100%), Positives = 267/267 (100%) Query: 1 MALNDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQIDETINQLVDSTSPSTIGIE 60 MALNDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQIDETINQLVDSTSPSTIGIE Sbjct: 1 MALNDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQIDETINQLVDSTSPSTIGIE 60 Query: 61 EQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTAP 120 EQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTAP Sbjct: 61 EQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTAP 120 Query: 121 KLSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPADEDDLQIEGGKI 180 KLSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPADEDDLQIEGGKI Sbjct: 121 KLSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPADEDDLQIEGGKI 180 Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPI 240 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPI Sbjct: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPI 240 Query: 241 MELRCKIAKMKESQEQDKRSSQAIDVL 267 MELRCKIAKMKESQEQDKRSSQAIDVL Sbjct: 241 MELRCKIAKMKESQEQDKRSSQAIDVL 267 >2YU4-A nrPDB Length = 94 Score = 41.2 bits (95), Expect = 4e-04, Method: Composition-based stats. Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 5/66 (7%) Query: 178 GKIELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRD----CPQAACSQV-VSM 232 G TCPIT + + P+ ++ C H ++ D I ++ R CPQ CS + Sbjct: 4 GSSGFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRK 63 Query: 233 RDFVRD 238 D ++D Sbjct: 64 SDLIQD 69 >5OLM-A nrPDB Length = 132 Score = 33.5 bits (75), Expect = 0.11, Method: Composition-based stats. Identities = 22/83 (26%), Positives = 40/83 (48%), Gaps = 6/83 (7%) Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPI 240 E+TCPI P+ P +S +C H F ++ I +G + CP C Q +++ P Sbjct: 16 EVTCPICLDPFVEP-VSIECGHSFCQECISQVGKGGGSV-CP--VCRQRFLLKNL--RPN 69 Query: 241 MELRCKIAKMKESQEQDKRSSQA 263 +L + +KE ++ + +Q Sbjct: 70 RQLANMVNNLKEISQEAREGTQG 92 >5OC1-A nrPDB Length = 565 Score = 31.2 bits (69), Expect = 0.49, Method: Composition-based stats. Identities = 32/123 (26%), Positives = 48/123 (39%), Gaps = 34/123 (27%) Query: 94 FKQSSDACPQIDLSTWDKYRTGELTAPKLSEL-YLNMPT--------PEPATMVNNTDTL 144 F+ SS+ +DL W RTG LTA + L +L +P+ P+PA N+ Sbjct: 331 FRDSSEF--NVDLDQWTNTRTGPLTALIANHLAWLRLPSNSSIFQTFPDPAAGPNSAHWE 388 Query: 145 KILKVLPYIWNDPTCVIPD----------LQNPADEDDLQIEGGKIELTCPITCKPYEAP 194 I W P PD L +P D+++ T P++ P Sbjct: 389 TIFS---NQWFHPAIPRPDTGSFMSVTNALISPVARGDIKLA----------TSNPFDKP 435 Query: 195 LIS 197 LI+ Sbjct: 436 LIN 438 >5KDR-A nrPDB Length = 327 Score = 30.1 bits (66), Expect = 0.93, Method: Composition-based stats. Identities = 17/38 (44%), Positives = 25/38 (65%), Gaps = 2/38 (5%) Query: 231 SMRDFVRDPIMELRCKIAKMKESQEQ-DKRSSQAIDVL 267 SM DF + P+ E+R KI +KESQ++ D + ID+L Sbjct: 13 SMLDFEK-PLFEIRNKIESLKESQDKNDVDLQEEIDML 49 >4R8P-L nrPDB Length = 268 Score = 30.1 bits (66), Expect = 1.1, Method: Composition-based stats. Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDP 239 EL CPI + + +++C H F D I L+ ++CP ++VS R DP Sbjct: 48 ELMCPICLDMLKNTMTTKECLHRFCADCIITALRS-GNKECPTCR-KKLVSKRSLRPDP 104 >2CKL-B nrPDB Length = 165 Score = 30.1 bits (66), Expect = 1.1, Method: Composition-based stats. Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDP 239 EL CPI + + +++C H F D I L+ ++CP ++VS R DP Sbjct: 54 ELMCPICLDMLKNTMTTKECLHRFCADCIITALRS-GNKECPTCR-KKLVSKRSLRPDP 110 >4BY6-A nrPDB Length = 553 Score = 30.1 bits (66), Expect = 1.2, Method: Composition-based stats. Identities = 15/48 (31%), Positives = 23/48 (47%) Query: 122 LSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPAD 169 LS + NM P P + N + + K LP ++ DP + L+ P D Sbjct: 307 LSAIPKNMTVPNPYDVDLNMEDIPACKELPEVFFDPVIDLHSLKKPVD 354 >4S3O-B nrPDB Length = 118 Score = 29.7 bits (65), Expect = 1.2, Method: Composition-based stats. Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Query: 181 ELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDP 239 EL CPI + + +++C H F D I L+ ++CP ++VS R DP Sbjct: 50 ELMCPICLDMLKNTMTTKECLHRFCADCIITALRS-GNKECPTCR-KKLVSKRSLRPDP 106 >2QKK-A nrPDB Length = 154 Score = 28.9 bits (63), Expect = 2.3, Method: Composition-based stats. Identities = 12/36 (33%), Positives = 21/36 (58%) Query: 201 NHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFV 236 N +F +GI N++QG+ +A +V++ DFV Sbjct: 78 NSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFV 113 >5Z56-q nrPDB Length = 504 Score = 28.5 bits (62), Expect = 3.3, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 20/34 (58%) Query: 180 IELTCPITCKPYEAPLISRKCNHVFDRDGIQNYL 213 + L C I+ + E P +S NHV++R I+ Y+ Sbjct: 1 MSLICSISNEVPEHPCVSPVSNHVYERRLIEKYI 34 >4CEM-A nrPDB Length = 370 Score = 27.7 bits (60), Expect = 5.0, Method: Composition-based stats. Identities = 24/107 (22%), Positives = 51/107 (47%), Gaps = 7/107 (6%) Query: 9 PKSVPLHPKSGKYFHNL---HARDLSNIYQQCYKQIDETINQ---LVDSTSPSTIGIEEQ 62 P S + P+ + F NL + L+ ++ ++++ T Q ++ S + +Q Sbjct: 223 PPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQ 282 Query: 63 VADITSTY-KLLSTYESESNSFDEHIKDLKKNFKQSSDACPQIDLST 108 + +Y KLL+ +S ++ DE++ DL ++ + P ID+ T Sbjct: 283 YEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFT 329 >4ARE-A nrPDB Length = 695 Score = 27.4 bits (59), Expect = 7.6, Method: Composition-based stats. Identities = 18/82 (21%), Positives = 38/82 (46%), Gaps = 4/82 (4%) Query: 38 YKQIDETINQLVDSTSPSTIGIEEQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQS 97 YK+ E +++ + S + + + + +T+ L TY E++ + KD + K+ Sbjct: 591 YKKASEVYSEISKAASLTNTSVTAEKSQYFNTFTLRGTYTGETSKGE--FKDWDEMSKKL 648 Query: 98 SDACPQIDLSTWDKYRTGELTA 119 + ++W Y+T LTA Sbjct: 649 DGTLESLAKNSWSGYKT--LTA 668 >2Y6I-A nrPDB Length = 785 Score = 27.4 bits (59), Expect = 7.6, Method: Composition-based stats. Identities = 18/82 (21%), Positives = 38/82 (46%), Gaps = 4/82 (4%) Query: 38 YKQIDETINQLVDSTSPSTIGIEEQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQS 97 YK+ E +++ + S + + + + +T+ L TY E++ + KD + K+ Sbjct: 591 YKKASEVYSEISKAASLTNTSVTAEKSQYFNTFTLRGTYTGETSKGE--FKDWDEMSKKL 648 Query: 98 SDACPQIDLSTWDKYRTGELTA 119 + ++W Y+T LTA Sbjct: 649 DGTLESLAKNSWSGYKT--LTA 668 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.132 0.393 Lambda K H 0.267 0.0409 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,955,442 Number of Sequences: 36641 Number of extensions: 451456 Number of successful extensions: 1077 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 26 Number of HSP's that attempted gapping in prelim test: 1065 Number of HSP's gapped (non-prelim): 38 length of query: 267 length of database: 10,336,785 effective HSP length: 95 effective length of query: 172 effective length of database: 6,855,890 effective search space: 1179213080 effective search space used: 1179213080 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 58 (27.0 bits)