BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38336 (279 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AGB-D nrPDB 569 e-163 6AHU-D nrPDB 70 1e-12 4FSJ-A nrPDB 31 0.64 4FTE-A nrPDB 30 1.0 3J4Q-A nrPDB 30 1.2 2BBV-A nrPDB 30 1.7 5C32-B nrPDB 29 2.2 2R0Q-C nrPDB 28 4.2 5WLC-NI nrPDB 27 7.2 >6AGB-D nrPDB Length = 279 Score = 569 bits (1466), Expect = e-163, Method: Compositional matrix adjust. Identities = 279/279 (100%), Positives = 279/279 (100%) Query: 1 MDRTQTFIKDCLFTKCLEDPEKPFNENRFQDTLLLLPTDGGLTSRLQRQQRKSKLNLDNL 60 MDRTQTFIKDCLFTKCLEDPEKPFNENRFQDTLLLLPTDGGLTSRLQRQQRKSKLNLDNL Sbjct: 1 MDRTQTFIKDCLFTKCLEDPEKPFNENRFQDTLLLLPTDGGLTSRLQRQQRKSKLNLDNL 60 Query: 61 QKVSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLH 120 QKVSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLH Sbjct: 61 QKVSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLH 120 Query: 121 YIEEKHPTIYESLPQYVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMADYNG 180 YIEEKHPTIYESLPQYVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMADYNG Sbjct: 121 YIEEKHPTIYESLPQYVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMADYNG 180 Query: 181 ALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDD 240 ALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDD Sbjct: 181 ALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDD 240 Query: 241 DSALRYSILGDRFKYRSVDRAGRKFKSRRCDDMLYYIQN 279 DSALRYSILGDRFKYRSVDRAGRKFKSRRCDDMLYYIQN Sbjct: 241 DSALRYSILGDRFKYRSVDRAGRKFKSRRCDDMLYYIQN 279 >6AHU-D nrPDB Length = 220 Score = 70.1 bits (170), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 47/139 (33%), Positives = 77/139 (55%), Gaps = 13/139 (9%) Query: 135 QYVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMADYNGALLRVTKSKNKTLI 194 +Y F+P++ ELW YI++L + K + KL AD +GA++ VTKSK + + Sbjct: 95 RYSLFLPLH-ELWKQYIRDLCSGLKP--DTQPQMIQAKLLKADLHGAIISVTKSKCPSYV 151 Query: 195 GLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDDDSALRYSILGDRFK 254 G+ GI++ +++ F +I K D +K IPK VF E D + Y I G +F+ Sbjct: 152 GITGILLQETKHIFKIITKE---DRLKVIPKLNCVFTVET------DGFISY-IYGSKFQ 201 Query: 255 YRSVDRAGRKFKSRRCDDM 273 RS +R+ +KFK++ D+ Sbjct: 202 LRSSERSAKKFKAKGTIDL 220 >4FSJ-A nrPDB Length = 363 Score = 30.8 bits (68), Expect = 0.64, Method: Compositional matrix adjust. Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 5/69 (7%) Query: 158 TKNLKTFNGSLAL----LKLSMADYNGALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVK 213 T NL F GS+ + +KLS + A T S TL+GL G++ F +K Sbjct: 178 TSNLMQFAGSITVWKCPVKLSTVQFPVATDPATSSLVHTLVGLDGVLAVGPDNFSESFIK 237 Query: 214 GNIIDEIKC 222 G + + C Sbjct: 238 G-VFSQSAC 245 >4FTE-A nrPDB Length = 407 Score = 30.0 bits (66), Expect = 1.0, Method: Compositional matrix adjust. Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 5/69 (7%) Query: 158 TKNLKTFNGSLAL----LKLSMADYNGALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVK 213 T NL F GS+ + +KLS + A T S TL+GL G++ F +K Sbjct: 178 TSNLMQFAGSITVWKCPVKLSTVQFPVATDPATSSLVHTLVGLDGVLAVGPDNFSESFIK 237 Query: 214 GNIIDEIKC 222 G + + C Sbjct: 238 G-VFSQSAC 245 >3J4Q-A nrPDB Length = 353 Score = 30.0 bits (66), Expect = 1.2, Method: Compositional matrix adjust. Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 1/48 (2%) Query: 160 NLKTFNGSLALLKLSMADYNGALLRVTKSKNKTLIGLQGIVIWDSQKF 207 + TF G + +KL+ D+ ALL + ++ +T +GI+ +S+ F Sbjct: 175 GIGTFQGQVGFVKLADGDHVSALLEIAETAKRTFQ-EKGILAGESRTF 221 >2BBV-A nrPDB Length = 363 Score = 29.6 bits (65), Expect = 1.7, Method: Compositional matrix adjust. Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 4/61 (6%) Query: 158 TKNLKTFNGSLAL----LKLSMADYNGALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVK 213 T NL F GS+ + +KLS + A T + TL+GL G++ F +K Sbjct: 178 TSNLMQFAGSITVWKCPVKLSNVQFPVATTPATSALVHTLVGLDGVLAVGPDNFSESFIK 237 Query: 214 G 214 G Sbjct: 238 G 238 >5C32-B nrPDB Length = 128 Score = 29.3 bits (64), Expect = 2.2, Method: Compositional matrix adjust. Identities = 11/23 (47%), Positives = 15/23 (65%) Query: 243 ALRYSILGDRFKYRSVDRAGRKF 265 AL + +GDRF S+DR GR + Sbjct: 49 ALNFVEMGDRFIVESIDRLGRNY 71 >2R0Q-C nrPDB Length = 209 Score = 28.1 bits (61), Expect = 4.2, Method: Compositional matrix adjust. Identities = 11/23 (47%), Positives = 15/23 (65%) Query: 243 ALRYSILGDRFKYRSVDRAGRKF 265 AL + +GDRF S+DR GR + Sbjct: 49 ALNFVRMGDRFIVESIDRLGRNY 71 >5WLC-NI nrPDB Length = 297 Score = 27.3 bits (59), Expect = 7.2, Method: Compositional matrix adjust. Identities = 39/134 (29%), Positives = 55/134 (41%), Gaps = 26/134 (19%) Query: 63 VSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLHYI 122 S L S+ EKR R K INNC KK Y N + Sbjct: 104 TSDLMSSTDVNEKRYTPRNTALLKFVDAASINNCWNALKK-----YSN-----------L 147 Query: 123 EEKHPT-IYE---SLPQYVDFVPMYKELWINYIKELLNITKNLKTFNGSLAL----LKLS 174 KHP ++E + P + FV YK L I+Y+KE +I ++ F A ++ S Sbjct: 148 HAKHPNELFEWTYTTPSFTTFVNFYKPLDIDYLKE--DIHTHMAIFEQREAQAQEDVQSS 205 Query: 175 MADYNGALLRVTKS 188 + D +G L V K+ Sbjct: 206 IVDEDGFTLVVGKN 219 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AGB-D nrPDB 494 e-140 6AHU-D nrPDB 187 4e-48 Sequences not found previously or not previously below threshold: 2KI7-A nrPDB 38 0.004 1TSF-A nrPDB 38 0.005 1PC0-A nrPDB 34 0.089 2D7R-A nrPDB 30 0.91 5NQA-A nrPDB 30 1.3 1SMQ-A nrPDB 29 2.3 5OQM-k nrPDB 28 4.3 4YU5-A nrPDB 28 5.9 1SXJ-C nrPDB 27 6.9 CONVERGED! >6AGB-D nrPDB Length = 279 Score = 494 bits (1271), Expect = e-140, Method: Composition-based stats. Identities = 279/279 (100%), Positives = 279/279 (100%) Query: 1 MDRTQTFIKDCLFTKCLEDPEKPFNENRFQDTLLLLPTDGGLTSRLQRQQRKSKLNLDNL 60 MDRTQTFIKDCLFTKCLEDPEKPFNENRFQDTLLLLPTDGGLTSRLQRQQRKSKLNLDNL Sbjct: 1 MDRTQTFIKDCLFTKCLEDPEKPFNENRFQDTLLLLPTDGGLTSRLQRQQRKSKLNLDNL 60 Query: 61 QKVSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLH 120 QKVSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLH Sbjct: 61 QKVSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLH 120 Query: 121 YIEEKHPTIYESLPQYVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMADYNG 180 YIEEKHPTIYESLPQYVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMADYNG Sbjct: 121 YIEEKHPTIYESLPQYVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMADYNG 180 Query: 181 ALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDD 240 ALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDD Sbjct: 181 ALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDD 240 Query: 241 DSALRYSILGDRFKYRSVDRAGRKFKSRRCDDMLYYIQN 279 DSALRYSILGDRFKYRSVDRAGRKFKSRRCDDMLYYIQN Sbjct: 241 DSALRYSILGDRFKYRSVDRAGRKFKSRRCDDMLYYIQN 279 >6AHU-D nrPDB Length = 220 Score = 187 bits (475), Expect = 4e-48, Method: Composition-based stats. Identities = 62/232 (26%), Positives = 113/232 (48%), Gaps = 41/232 (17%) Query: 63 VSQLESADKQLEKRDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLHYI 122 +SQ E+ D ++ QR ++ +R ++ K++T + A E+++ K +L Y Sbjct: 9 LSQKEANDSDVQPSGAQR----AEAFVRAFL---KRSTPRMSPQAREDQLQRKAVVLEYF 61 Query: 123 ------------------EEKHPTIYESLP---QYVDFVPMYKELWINYIKELLNITKNL 161 + + +++ P +Y F+P++ ELW YI++L + K Sbjct: 62 TRHKRKEKKKKAKGLSARQRRELRLFDIKPEQQRYSLFLPLH-ELWKQYIRDLCSGLKP- 119 Query: 162 KTFNGSLALLKLSMADYNGALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIK 221 + KL AD +GA++ VTKSK + +G+ GI++ +++ F +I K D +K Sbjct: 120 -DTQPQMIQAKLLKADLHGAIISVTKSKCPSYVGITGILLQETKHIFKIITKE---DRLK 175 Query: 222 CIPKKGTVFQFEIPISDDDDSALRYSILGDRFKYRSVDRAGRKFKSRRCDDM 273 IPK VF E D + Y I G +F+ RS +R+ +KFK++ D+ Sbjct: 176 VIPKLNCVFTVET------DGFISY-IYGSKFQLRSSERSAKKFKAKGTIDL 220 >2KI7-A nrPDB Length = 127 Score = 38.1 bits (87), Expect = 0.004, Method: Composition-based stats. Identities = 18/90 (20%), Positives = 41/90 (45%), Gaps = 10/90 (11%) Query: 177 DYNGALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPI 236 + G +RV S + +G++G VI +++ ++ + + +PK +F+FE Sbjct: 47 ELIGLKVRVVNSTHPGYVGIEGYVIDETRNMLVIAGENKVWK----VPKDVCIFEFETW- 101 Query: 237 SDDDDSALRYSILGDRFKYRSVDRAGRKFK 266 D + I G++ R R ++++ Sbjct: 102 ----DGT-KIKISGEKLVGRPEMRLKKRWR 126 >1TSF-A nrPDB Length = 102 Score = 38.1 bits (87), Expect = 0.005, Method: Composition-based stats. Identities = 23/99 (23%), Positives = 44/99 (44%), Gaps = 11/99 (11%) Query: 165 NGSLALLKLSMADYNGALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIP 224 G L ++L D+ G ++ V +S N + +G++G V+ ++Q ++ + +K + Sbjct: 2 RGRLQGVELIARDWIGLMVEVVESPNHSEVGIKGEVVDETQNTLKIMTEKG----LKVVA 57 Query: 225 KKGTVFQFEIPISDDDDSALRYSILGDRFKYRSVDRAGR 263 K+G F+ I GD +R DR R Sbjct: 58 KRGRTFRVWYKGKI-------MRIKGDLINFRPEDRIKR 89 >1PC0-A nrPDB Length = 61 Score = 33.9 bits (76), Expect = 0.089, Method: Composition-based stats. Identities = 12/53 (22%), Positives = 29/53 (54%), Gaps = 4/53 (7%) Query: 180 GALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQF 232 G ++ V +S N + +G++G V+ ++Q ++ + +K + K+G F+ Sbjct: 1 GLMVEVVESPNHSEVGIKGEVVDETQNTLKIMTEKG----LKVVAKRGRTFRV 49 >2D7R-A nrPDB Length = 570 Score = 30.4 bits (67), Expect = 0.91, Method: Composition-based stats. Identities = 19/96 (19%), Positives = 44/96 (45%), Gaps = 9/96 (9%) Query: 70 DKQLEKRDYQRINKNSKIALREY-INNCKKNTKKCLKLAYENKITDKEDLLHYIEE-KHP 127 DK+ +RD QR+ + R Y + + ++ + + + ++DK L + + +HP Sbjct: 43 DKEAIRRDAQRVGNGEQ--GRPYPMTDAERVDQAYRENGFNIYVSDKISLNRSLPDIRHP 100 Query: 128 TI-----YESLPQYVDFVPMYKELWINYIKELLNIT 158 E+LP +P + E W + ++ + ++ Sbjct: 101 NCNSKRYLETLPNTSIIIPFHNEGWSSLLRTVHSVL 136 >5NQA-A nrPDB Length = 578 Score = 30.0 bits (66), Expect = 1.3, Method: Composition-based stats. Identities = 20/102 (19%), Positives = 44/102 (43%), Gaps = 13/102 (12%) Query: 83 KNSKIALREYINNCKKNTKKCLKLAYENKITDKEDLLHYIEEK-------HPTIYESLPQ 135 K SK+ L E + K+ + + A ++D+ L +IE+K Y +LP Sbjct: 79 KASKLQLNE--DELKQQEELIERYAINIYLSDRISLHRHIEDKRMYECKSQKFNYRTLPT 136 Query: 136 YVDFVPMYKELWINYIKELLNITKNLKTFNGSLALLKLSMAD 177 + Y E W ++ + ++ + + ++ L ++ + D Sbjct: 137 TSVIIAFYNEAWSTLLRTIHSVLE----TSPAVLLKEIILVD 174 >1SMQ-A nrPDB Length = 399 Score = 28.9 bits (63), Expect = 2.3, Method: Composition-based stats. Identities = 27/121 (22%), Positives = 56/121 (46%), Gaps = 17/121 (14%) Query: 57 LDNLQKVSQLESADKQLEK-RDYQRINKNSKIALREYINNCKKNTKKCLKLAYENKITDK 115 L +L+ + +K+LE R+ R+ + L+E ++ +N K LK ++ K Sbjct: 14 LSDLEIKDSKSNLNKELETLREENRVKSD---MLKEKLSKDAENHKAYLKSHQVHRHKLK 70 Query: 116 EDLLHYIEEKHPTIYESLPQYVDFVPMYKELWINYIK--------ELLNITKNLKTFNGS 167 E +E++ P + E + V F Y E+W Y + E ++++K++ +N Sbjct: 71 E-----MEKEEPLLNEDKERTVLFPIKYHEIWQAYKRAEASFWTAEEIDLSKDIHDWNNR 125 Query: 168 L 168 + Sbjct: 126 M 126 >5OQM-k nrPDB Length = 157 Score = 28.1 bits (61), Expect = 4.3, Method: Composition-based stats. Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 10/85 (11%) Query: 41 GLTSRLQRQ-QRKSKLNLDNLQKVSQLESADKQLEKRDYQRINKNSKIALREYINNCKKN 99 G+ + LQR R KLN+ + S L D LE Y +N I RE++ +++ Sbjct: 48 GMITNLQRNVDRLYKLNVRSNDPQSSLSKVDIPLEVVQYIEDGRNPDIYTREFVEAIRRS 107 Query: 100 TK---------KCLKLAYENKITDK 115 + K L+ + +KI D+ Sbjct: 108 NQYQRGKMHGLKQLRDSLADKIVDE 132 >4YU5-A nrPDB Length = 756 Score = 27.7 bits (60), Expect = 5.9, Method: Composition-based stats. Identities = 15/53 (28%), Positives = 21/53 (39%), Gaps = 3/53 (5%) Query: 192 TLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPKKGTVFQFEIPISDDDDSAL 244 GLQG+ +D K + I D + +PK G FQ D A+ Sbjct: 697 NYSGLQGVTTFDDSKVY---SNNQIADAGRKVPKLGLKFQVVGQADDKSAGAV 746 >1SXJ-C nrPDB Length = 340 Score = 27.3 bits (59), Expect = 6.9, Method: Composition-based stats. Identities = 29/106 (27%), Positives = 44/106 (41%), Gaps = 15/106 (14%) Query: 88 ALREYINNCKKNTKKCLKLAYENKITDKEDLLHYIEEKHPTIYESLPQYVDFVPMYKELW 147 ALR I KNT+ C+ Y +K+T P + ++ F P+ +E Sbjct: 129 ALRRVIERYTKNTRFCVLANYAHKLT-------------PALLSQCTRFR-FQPLPQEAI 174 Query: 148 INYIKELLNITKNLKTFNGSLALLKLSMADYNGALLRVTKSKNKTL 193 I +L K + N AL++LS D +L V +S TL Sbjct: 175 ERRIANVLVHEKLKLSPNAEKALIELSNGDMR-RVLNVLQSCKATL 219 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.134 0.372 Lambda K H 0.267 0.0414 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,378,921 Number of Sequences: 36641 Number of extensions: 403889 Number of successful extensions: 1172 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 25 Number of HSP's that attempted gapping in prelim test: 1154 Number of HSP's gapped (non-prelim): 39 length of query: 279 length of database: 10,336,785 effective HSP length: 96 effective length of query: 183 effective length of database: 6,819,249 effective search space: 1247922567 effective search space used: 1247922567 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.9 bits)