BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38291 (140 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AH3-G nrPDB 284 7e-78 3V8B-A nrPDB 30 0.27 3JAX-A nrPDB 28 1.1 1BR4-A nrPDB 28 1.4 4CJ1-A nrPDB 27 2.2 5N6W-A nrPDB 27 2.5 6BPM-C nrPDB 27 3.2 4DD5-A nrPDB 25 8.0 >6AH3-G nrPDB Length = 140 Score = 284 bits (727), Expect = 7e-78, Method: Compositional matrix adjust. Identities = 140/140 (100%), Positives = 140/140 (100%) Query: 1 MALKKNTHNKSTKRVTKHPSLKTLTHKQIHTTIFVKSTTPYVSALKRINKFLDSVHKQGS 60 MALKKNTHNKSTKRVTKHPSLKTLTHKQIHTTIFVKSTTPYVSALKRINKFLDSVHKQGS Sbjct: 1 MALKKNTHNKSTKRVTKHPSLKTLTHKQIHTTIFVKSTTPYVSALKRINKFLDSVHKQGS 60 Query: 61 SYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVITEGQADIDMESDVEDD 120 SYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVITEGQADIDMESDVEDD Sbjct: 61 SYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVITEGQADIDMESDVEDD 120 Query: 121 DKETQLKKRAVSGVELRIYV 140 DKETQLKKRAVSGVELRIYV Sbjct: 121 DKETQLKKRAVSGVELRIYV 140 >3V8B-A nrPDB Length = 283 Score = 30.4 bits (67), Expect = 0.27, Method: Compositional matrix adjust. Identities = 19/73 (26%), Positives = 31/73 (42%), Gaps = 1/73 (1%) Query: 49 NKFLDSVHKQGSSYVAVLGMGKAVEKTLALGCHFQDQKNKKI-EVYTKTIEVLDEVITEG 107 N + S+ Q S + G G + + AL + T+ EV DE++ G Sbjct: 17 NLYFQSMMNQPSPVALITGAGSGIGRATALALAADGVTVGALGRTRTEVEEVADEIVGAG 76 Query: 108 QADIDMESDVEDD 120 I +E+DV D+ Sbjct: 77 GQAIALEADVSDE 89 >3JAX-A nrPDB Length = 974 Score = 28.5 bits (62), Expect = 1.1, Method: Compositional matrix adjust. Identities = 10/25 (40%), Positives = 17/25 (68%) Query: 45 LKRINKFLDSVHKQGSSYVAVLGMG 69 L R+NK LD +QG+S++ +L + Sbjct: 443 LTRVNKALDKTKRQGASFLGILDIA 467 >1BR4-A nrPDB Length = 820 Score = 28.1 bits (61), Expect = 1.4, Method: Compositional matrix adjust. Identities = 10/22 (45%), Positives = 16/22 (72%) Query: 45 LKRINKFLDSVHKQGSSYVAVL 66 L R+NK LD +QG+S++ +L Sbjct: 442 LTRVNKALDKTKRQGASFLGIL 463 >4CJ1-A nrPDB Length = 625 Score = 27.3 bits (59), Expect = 2.2, Method: Composition-based stats. Identities = 13/42 (30%), Positives = 25/42 (59%) Query: 52 LDSVHKQGSSYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVY 93 SV+++G+ Y+AV G+GK+V +A+ + K + +Y Sbjct: 86 FSSVNEEGTYYLAVPGVGKSVNFKIAMNVYEDAFKTAMLGMY 127 >5N6W-A nrPDB Length = 201 Score = 27.3 bits (59), Expect = 2.5, Method: Compositional matrix adjust. Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 9/57 (15%) Query: 60 SSYVAVLGMGKAVEKTLALGCH----FQDQKNKKIEVYTKTIEVLDEVITEGQADID 112 SS+ A KA + GC FQD ++ +++ K I+V+ ++T+G DID Sbjct: 71 SSWTA----NKARLNSQGFGCAWLSKFQD-SSQWLQIDLKEIKVISGILTQGHCDID 122 >6BPM-C nrPDB Length = 727 Score = 26.9 bits (58), Expect = 3.2, Method: Composition-based stats. Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 10/80 (12%) Query: 63 VAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDE--VITEG--QADIDMESDVE 118 +A G G + +T F+ QK E+ TK +VLD+ ++T + DI+ E + Sbjct: 514 LAQSGSGNSANRT-----DFKPQKANTSEIGTKW-QVLDKRLLLTAALFRTDIENEVEQN 567 Query: 119 DDDKETQLKKRAVSGVELRI 138 DD +Q K+ V G E+ + Sbjct: 568 DDGTYSQYGKKRVEGYEISV 587 >4DD5-A nrPDB Length = 396 Score = 25.4 bits (54), Expect = 8.0, Method: Compositional matrix adjust. Identities = 19/81 (23%), Positives = 36/81 (44%), Gaps = 5/81 (6%) Query: 44 ALKRINKFLDSVHKQGSSYVAVLGMGKAVEKTLALGCHFQDQK-----NKKIEVYTKTIE 98 A+KR N D + + V G+G+ + + +ALG +K N +++ Sbjct: 42 AIKRANITPDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVS 101 Query: 99 VLDEVITEGQADIDMESDVED 119 + ++I G ADI + E+ Sbjct: 102 MASQLIALGDADIMLVGGAEN 122 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AH3-G nrPDB 259 2e-70 Sequences not found previously or not previously below threshold: 3V8B-A nrPDB 29 0.51 6BPM-C nrPDB 29 0.81 3JAX-A nrPDB 29 0.95 1BR4-A nrPDB 29 0.95 4CJ1-A nrPDB 28 1.3 4RAJ-A nrPDB 27 3.2 3I56-L nrPDB 27 3.7 2OKT-A nrPDB 26 4.6 3HJA-A nrPDB 26 6.8 2OHF-A nrPDB 25 9.1 CONVERGED! >6AH3-G nrPDB Length = 140 Score = 259 bits (662), Expect = 2e-70, Method: Composition-based stats. Identities = 140/140 (100%), Positives = 140/140 (100%) Query: 1 MALKKNTHNKSTKRVTKHPSLKTLTHKQIHTTIFVKSTTPYVSALKRINKFLDSVHKQGS 60 MALKKNTHNKSTKRVTKHPSLKTLTHKQIHTTIFVKSTTPYVSALKRINKFLDSVHKQGS Sbjct: 1 MALKKNTHNKSTKRVTKHPSLKTLTHKQIHTTIFVKSTTPYVSALKRINKFLDSVHKQGS 60 Query: 61 SYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVITEGQADIDMESDVEDD 120 SYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVITEGQADIDMESDVEDD Sbjct: 61 SYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVITEGQADIDMESDVEDD 120 Query: 121 DKETQLKKRAVSGVELRIYV 140 DKETQLKKRAVSGVELRIYV Sbjct: 121 DKETQLKKRAVSGVELRIYV 140 >3V8B-A nrPDB Length = 283 Score = 29.3 bits (64), Expect = 0.51, Method: Composition-based stats. Identities = 19/73 (26%), Positives = 31/73 (42%), Gaps = 1/73 (1%) Query: 49 NKFLDSVHKQGSSYVAVLGMGKAVEKTLALGCHFQDQKNKKI-EVYTKTIEVLDEVITEG 107 N + S+ Q S + G G + + AL + T+ EV DE++ G Sbjct: 17 NLYFQSMMNQPSPVALITGAGSGIGRATALALAADGVTVGALGRTRTEVEEVADEIVGAG 76 Query: 108 QADIDMESDVEDD 120 I +E+DV D+ Sbjct: 77 GQAIALEADVSDE 89 >6BPM-C nrPDB Length = 727 Score = 28.9 bits (63), Expect = 0.81, Method: Composition-based stats. Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 10/80 (12%) Query: 63 VAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDE--VITEG--QADIDMESDVE 118 +A G G + +T F+ QK E+ TK +VLD+ ++T + DI+ E + Sbjct: 514 LAQSGSGNSANRT-----DFKPQKANTSEIGTKW-QVLDKRLLLTAALFRTDIENEVEQN 567 Query: 119 DDDKETQLKKRAVSGVELRI 138 DD +Q K+ V G E+ + Sbjct: 568 DDGTYSQYGKKRVEGYEISV 587 >3JAX-A nrPDB Length = 974 Score = 28.6 bits (62), Expect = 0.95, Method: Composition-based stats. Identities = 10/22 (45%), Positives = 16/22 (72%) Query: 45 LKRINKFLDSVHKQGSSYVAVL 66 L R+NK LD +QG+S++ +L Sbjct: 443 LTRVNKALDKTKRQGASFLGIL 464 >1BR4-A nrPDB Length = 820 Score = 28.6 bits (62), Expect = 0.95, Method: Composition-based stats. Identities = 10/22 (45%), Positives = 16/22 (72%) Query: 45 LKRINKFLDSVHKQGSSYVAVL 66 L R+NK LD +QG+S++ +L Sbjct: 442 LTRVNKALDKTKRQGASFLGIL 463 >4CJ1-A nrPDB Length = 625 Score = 28.2 bits (61), Expect = 1.3, Method: Composition-based stats. Identities = 24/87 (27%), Positives = 42/87 (48%), Gaps = 11/87 (12%) Query: 9 NKSTKRVTKHPSLKTLTHKQIHTTIFVKST--TPYVSALKRINKFLDSVHKQGSSYVAVL 66 N ST V K T+ + T++F T T Y++ SV+++G+ Y+AV Sbjct: 50 NCSTFYVVKEDG--TIVYTGTATSMFDNDTKETVYIADFS-------SVNEEGTYYLAVP 100 Query: 67 GMGKAVEKTLALGCHFQDQKNKKIEVY 93 G+GK+V +A+ + K + +Y Sbjct: 101 GVGKSVNFKIAMNVYEDAFKTAMLGMY 127 >4RAJ-A nrPDB Length = 247 Score = 27.0 bits (58), Expect = 3.2, Method: Composition-based stats. Identities = 21/75 (28%), Positives = 32/75 (42%), Gaps = 11/75 (14%) Query: 46 KRINKFLDSVHKQGSSYVAVLGMGKAVEKTL---ALGCHFQDQKNKKIEVYTKTIEVLDE 102 KR++K +H +S V + +K L ALGC + V+ LDE Sbjct: 22 KRLSKASRKIHNVSNSLVNARLIALFSDKDLYAKALGCFYY--------VFVALEAALDE 73 Query: 103 VITEGQADIDMESDV 117 + +G AD+ DV Sbjct: 74 ALKKGDADVSKFKDV 88 >3I56-L nrPDB Length = 165 Score = 26.6 bits (57), Expect = 3.7, Method: Composition-based stats. Identities = 16/63 (25%), Positives = 32/63 (50%), Gaps = 1/63 (1%) Query: 60 SSYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVITEGQADIDMESDVED 119 + YV VLG G+ + + F + +K+E ++E+ D + E QA+ + D + Sbjct: 104 ADYVKVLGAGQVRHELTLIADDFSEGAREKVEGAGGSVELTD-LGEERQAEAEETEDADA 162 Query: 120 DDK 122 D++ Sbjct: 163 DEE 165 >2OKT-A nrPDB Length = 342 Score = 26.2 bits (56), Expect = 4.6, Method: Composition-based stats. Identities = 19/76 (25%), Positives = 36/76 (47%), Gaps = 14/76 (18%) Query: 39 TPYVSALKRINKFLDSVHKQGSSYVAVLGMGKAVE------KTLALGCH-----FQDQKN 87 TP V+ R F++ + +G++Y G A + +T+A H F+D +N Sbjct: 22 TPKVTLTHRDCLFIELIDDKGNAY---FGECNAFQTDWYDHETIASVKHVIEQWFEDNRN 78 Query: 88 KKIEVYTKTIEVLDEV 103 K E Y ++++D + Sbjct: 79 KSFETYEAALKLVDSL 94 >3HJA-A nrPDB Length = 356 Score = 25.9 bits (55), Expect = 6.8, Method: Composition-based stats. Identities = 17/63 (26%), Positives = 29/63 (46%) Query: 22 KTLTHKQIHTTIFVKSTTPYVSALKRINKFLDSVHKQGSSYVAVLGMGKAVEKTLALGCH 81 K L ++ + ++ST + SA +LD V+ G+ V + K KT+ LG + Sbjct: 99 KNLPWAKLGIDVVIESTGVFSSATSDKGGYLDHVNHAGAKKVILTVPAKDEIKTIVLGVN 158 Query: 82 FQD 84 D Sbjct: 159 DHD 161 >2OHF-A nrPDB Length = 396 Score = 25.5 bits (54), Expect = 9.1, Method: Composition-based stats. Identities = 24/89 (26%), Positives = 34/89 (38%), Gaps = 6/89 (6%) Query: 51 FLDSVHKQGSSYVAVL------GMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVI 104 FL HK S A L G+ K LG F + ++ T D+ I Sbjct: 73 FLCQYHKPASKIPAFLNVVDIAGLVKGAHNGQGLGNAFLSHISACDGIFHLTRAFEDDDI 132 Query: 105 TEGQADIDMESDVEDDDKETQLKKRAVSG 133 T + +D D+E +E QLK + G Sbjct: 133 THVEGSVDPIRDIEIIHEELQLKDEEMIG 161 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.128 0.344 Lambda K H 0.267 0.0401 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,834,797 Number of Sequences: 36641 Number of extensions: 173350 Number of successful extensions: 470 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 18 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 450 Number of HSP's gapped (non-prelim): 39 length of query: 140 length of database: 10,336,785 effective HSP length: 87 effective length of query: 53 effective length of database: 7,149,018 effective search space: 378897954 effective search space used: 378897954 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 54 (25.4 bits)