BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38247 (162 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4XPM-C nrPDB 333 1e-92 4FUW-A nrPDB 305 5e-84 3T8S-A nrPDB 30 0.33 1P4X-A nrPDB 27 3.4 >4XPM-C nrPDB Length = 162 Score = 333 bits (855), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 162/162 (100%), Positives = 162/162 (100%) Query: 1 MVMLHSKNVKGFLENTLKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSI 60 MVMLHSKNVKGFLENTLKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSI Sbjct: 1 MVMLHSKNVKGFLENTLKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSI 60 Query: 61 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA 120 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA Sbjct: 61 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA 120 Query: 121 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG 162 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG Sbjct: 121 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG 162 >4FUW-A nrPDB Length = 161 Score = 305 bits (782), Expect = 5e-84, Method: Compositional matrix adjust. Identities = 152/162 (93%), Positives = 152/162 (93%), Gaps = 9/162 (5%) Query: 1 MVMLHSKNVKGFLENTLKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSI 60 MVMLHSKNVKGFLENTLKPY SLQSSMIITATNGGILSYATSNNDVPKNSI Sbjct: 1 MVMLHSKNVKGFLENTLKPY---------GSLQSSMIITATNGGILSYATSNNDVPKNSI 51 Query: 61 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA 120 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA Sbjct: 52 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA 111 Query: 121 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG 162 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG Sbjct: 112 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG 153 >3T8S-A nrPDB Length = 585 Score = 30.4 bits (67), Expect = 0.33, Method: Composition-based stats. Identities = 18/67 (26%), Positives = 35/67 (52%), Gaps = 1/67 (1%) Query: 20 YDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSINEINSVNNLKMMSLLIKDK 79 Y L S+ + + +++ N G +A+S+ V NE+NSVN +++ K Sbjct: 165 YKLRSIG-DSVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCNTSWKIVLFMK 223 Query: 80 WSEDEND 86 WS++++D Sbjct: 224 WSDNKDD 230 >1P4X-A nrPDB Length = 250 Score = 27.3 bits (59), Expect = 3.4, Method: Compositional matrix adjust. Identities = 17/66 (25%), Positives = 30/66 (45%), Gaps = 7/66 (10%) Query: 37 IITATNGGILSYATSNNDVPKNSINEINSVNNLKMMSLLIKDKWSEDE-------NDTEE 89 IIT+ N I+ + + ++NNLK LIK++ +EDE +D ++ Sbjct: 166 IITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQ 225 Query: 90 QHSNSC 95 H+ Sbjct: 226 DHAEQL 231 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4XPM-C nrPDB 305 4e-84 4FUW-A nrPDB 287 2e-78 Sequences not found previously or not previously below threshold: 3T8S-A nrPDB 32 0.13 4EGL-A nrPDB 27 3.2 1P4X-A nrPDB 26 6.4 1X1B-A nrPDB 26 7.9 1FNX-H nrPDB 26 9.9 CONVERGED! >4XPM-C nrPDB Length = 162 Score = 305 bits (782), Expect = 4e-84, Method: Composition-based stats. Identities = 162/162 (100%), Positives = 162/162 (100%) Query: 1 MVMLHSKNVKGFLENTLKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSI 60 MVMLHSKNVKGFLENTLKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSI Sbjct: 1 MVMLHSKNVKGFLENTLKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSI 60 Query: 61 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA 120 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA Sbjct: 61 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA 120 Query: 121 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG 162 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG Sbjct: 121 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG 162 >4FUW-A nrPDB Length = 161 Score = 287 bits (734), Expect = 2e-78, Method: Composition-based stats. Identities = 152/162 (93%), Positives = 152/162 (93%), Gaps = 9/162 (5%) Query: 1 MVMLHSKNVKGFLENTLKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSI 60 MVMLHSKNVKGFLENTLKPY SLQSSMIITATNGGILSYATSNNDVPKNSI Sbjct: 1 MVMLHSKNVKGFLENTLKPY---------GSLQSSMIITATNGGILSYATSNNDVPKNSI 51 Query: 61 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA 120 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA Sbjct: 52 NEINSVNNLKMMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVA 111 Query: 121 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG 162 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG Sbjct: 112 QIPNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYKLG 153 >3T8S-A nrPDB Length = 585 Score = 31.9 bits (71), Expect = 0.13, Method: Composition-based stats. Identities = 16/70 (22%), Positives = 35/70 (50%) Query: 17 LKPYDLHSVDFKTSSLQSSMIITATNGGILSYATSNNDVPKNSINEINSVNNLKMMSLLI 76 ++P+ + + +++ N G +A+S+ V NE+NSVN +++ Sbjct: 161 IQPFYKLRSIGDSVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCNTSWKIVL 220 Query: 77 KDKWSEDEND 86 KWS++++D Sbjct: 221 FMKWSDNKDD 230 >4EGL-A nrPDB Length = 177 Score = 27.3 bits (59), Expect = 3.2, Method: Composition-based stats. Identities = 24/119 (20%), Positives = 53/119 (44%), Gaps = 6/119 (5%) Query: 12 FLENTLKPYDLHSVDFKTSSLQSSMII-TATNGGILSYATSNNDVPKNSINEINSVNNLK 70 +L + +L S+ ++S+ +I G L Y N K++ IN++N L+ Sbjct: 9 YLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLR 68 Query: 71 MMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVAQIPNSDLLL 129 + S IK ++ ++ + + + I + ++ED+ + +I NS +L+ Sbjct: 69 LQSKTIKVSYARPSSEVIKDAN-----LYISGLPRTMTQKDVEDMFSRFGRIINSRVLV 122 >1P4X-A nrPDB Length = 250 Score = 26.1 bits (56), Expect = 6.4, Method: Composition-based stats. Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 7/63 (11%) Query: 37 IITATNGGILSYATSNNDVPKNSINEINSVNNLKMMSLLIKDKWSEDE-------NDTEE 89 IIT+ N I+ + + ++NNLK LIK++ +EDE +D ++ Sbjct: 166 IITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQ 225 Query: 90 QHS 92 H+ Sbjct: 226 DHA 228 >1X1B-A nrPDB Length = 359 Score = 26.1 bits (56), Expect = 7.9, Method: Composition-based stats. Identities = 12/38 (31%), Positives = 19/38 (50%) Query: 123 PNSDLLLLFIAEGSFPYGLLVIKIERAMRELTDLFGYK 160 PN D L +I P+ +L K + +E+ + GYK Sbjct: 304 PNFDYLSHYILGAGMPFSVLGFKEQARYKEILESLGYK 341 >1FNX-H nrPDB Length = 174 Score = 25.8 bits (55), Expect = 9.9, Method: Composition-based stats. Identities = 23/119 (19%), Positives = 48/119 (40%), Gaps = 6/119 (5%) Query: 12 FLENTLKPYDLHSVDFKTSSLQSSMII-TATNGGILSYATSNNDVPKNSINEINSVNNLK 70 +L + + S+ ++S ++ G L Y N P ++ IN++N LK Sbjct: 11 YLPQNMTQDEFKSLFGSIGDIESCKLVRDKITGQSLGYGFVNYSDPNDADKAINTLNGLK 70 Query: 71 MMSLLIKDKWSEDENDTEEQHSNSCYPVEIDSFKTKIYTYEMEDLHTCVAQIPNSDLLL 129 + + IK ++ + + + + + + EME L + +I S +LL Sbjct: 71 LQTKTIKVSYARPSSASIRDAN-----LYVSGLPKTMSQKEMEQLFSQYGRIITSRILL 124 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.137 0.402 Lambda K H 0.267 0.0396 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,936,641 Number of Sequences: 36641 Number of extensions: 216133 Number of successful extensions: 637 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 618 Number of HSP's gapped (non-prelim): 33 length of query: 162 length of database: 10,336,785 effective HSP length: 89 effective length of query: 73 effective length of database: 7,075,736 effective search space: 516528728 effective search space used: 516528728 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 55 (25.8 bits)