BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38208 (133 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AH3-H nrPDB 274 7e-75 5GM5-A nrPDB 31 0.18 3X0V-A nrPDB 28 1.3 2VA8-A nrPDB 28 1.3 3FOT-A nrPDB 27 1.9 1J0H-A nrPDB 27 2.3 6AJJ-A nrPDB 27 3.5 4MJ2-A nrPDB 27 3.8 4QRO-A nrPDB 26 4.3 3BVQ-A nrPDB 26 4.7 4HZV-A nrPDB 25 9.2 >6AH3-H nrPDB Length = 133 Score = 274 bits (701), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 133/133 (100%), Positives = 133/133 (100%) Query: 1 MGKKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLHV 60 MGKKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLHV Sbjct: 1 MGKKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLHV 60 Query: 61 DDKLAYIRVNHADKDTFSSSISTYISTDELVGSPLTVSILQESSSLRLLEVTDDDRLWLK 120 DDKLAYIRVNHADKDTFSSSISTYISTDELVGSPLTVSILQESSSLRLLEVTDDDRLWLK Sbjct: 61 DDKLAYIRVNHADKDTFSSSISTYISTDELVGSPLTVSILQESSSLRLLEVTDDDRLWLK 120 Query: 121 KVMEEEEQDCKCI 133 KVMEEEEQDCKCI Sbjct: 121 KVMEEEEQDCKCI 133 >5GM5-A nrPDB Length = 220 Score = 30.8 bits (68), Expect = 0.18, Method: Compositional matrix adjust. Identities = 21/81 (25%), Positives = 34/81 (41%), Gaps = 2/81 (2%) Query: 29 IDQMTWRQWLN-NALKRSYGIFGEGVEYSFLH-VDDKLAYIRVNHADKDTFSSSISTYIS 86 +D TW W N +++Y SF V+D Y+ NH + I+ Sbjct: 140 VDGQTWELWYGANGSQKTYSFVAPTPITSFQGDVNDFFKYLTQNHGFPASSQYLITLQFG 199 Query: 87 TDELVGSPLTVSILQESSSLR 107 T G P T+S+ S+S++ Sbjct: 200 TAPFTGGPATLSVSNWSASVQ 220 >3X0V-A nrPDB Length = 541 Score = 28.1 bits (61), Expect = 1.3, Method: Composition-based stats. Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 6/61 (9%) Query: 1 MGKKTFREWQYFKLSITS-FDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLH 59 +G+K + + Y+K + FD+ D+ +D MT R++L G GE +Y F Sbjct: 134 VGEKLQQAFGYYKEKLAEDFDKGFDELMLVDDMTTREYLKRG-----GPKGEAPKYDFFA 188 Query: 60 V 60 + Sbjct: 189 I 189 >2VA8-A nrPDB Length = 715 Score = 28.1 bits (61), Expect = 1.3, Method: Composition-based stats. Identities = 12/24 (50%), Positives = 18/24 (75%), Gaps = 2/24 (8%) Query: 5 TFREWQY--FKLSITSFDQDVDDA 26 TF++W+ FK+++TS D D DDA Sbjct: 94 TFKDWELIGFKVAMTSGDYDTDDA 117 >3FOT-A nrPDB Length = 519 Score = 27.3 bits (59), Expect = 1.9, Method: Compositional matrix adjust. Identities = 10/28 (35%), Positives = 16/28 (57%) Query: 33 TWRQWLNNALKRSYGIFGEGVEYSFLHV 60 ++RQWL N ++ G+ E S+LH Sbjct: 396 SYRQWLENPFLQALGLRVHNFEASYLHA 423 >1J0H-A nrPDB Length = 588 Score = 27.3 bits (59), Expect = 2.3, Method: Composition-based stats. Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Query: 41 ALKRSYGIFGEGVEYSFLHVDDKLAYIRVNHADKD 75 AL++ Y G E SFLH DD++ Y+ D D Sbjct: 495 ALRKQYRSLRRG-EISFLHADDEMNYLIYKKTDGD 528 >6AJJ-A nrPDB Length = 943 Score = 26.6 bits (57), Expect = 3.5, Method: Composition-based stats. Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 14/70 (20%) Query: 70 NHADKDTF----SSSISTYISTDELVGSPLTVSILQESSSLRLLEVTDD----DRLWLKK 121 NH + F S S++ + DE+ G T S + +L DD D+ W KK Sbjct: 49 NHVTQSGFYDEGSQSVAASLIGDEVYGRDRT------SHVVAILTPPDDKKVTDKAWQKK 102 Query: 122 VMEEEEQDCK 131 V EE +Q K Sbjct: 103 VTEELDQVVK 112 >4MJ2-A nrPDB Length = 653 Score = 26.6 bits (57), Expect = 3.8, Method: Composition-based stats. Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 7/62 (11%) Query: 16 ITSFDQDVDDAH--AIDQMTWRQ----WLNNALKRSYGIFGEGVEYSFLHVDDKLAYIRV 69 + S+DQ ++ A+ A+ +Q WL L + G G+G+ Y+F H+D L +R Sbjct: 65 VLSWDQQLNLAYVGAVPHRGIKQVRTHWLLE-LVTTRGSTGQGLSYNFTHLDGYLDLLRE 123 Query: 70 NH 71 N Sbjct: 124 NQ 125 >4QRO-A nrPDB Length = 348 Score = 26.2 bits (56), Expect = 4.3, Method: Compositional matrix adjust. Identities = 12/29 (41%), Positives = 17/29 (58%) Query: 41 ALKRSYGIFGEGVEYSFLHVDDKLAYIRV 69 ALK G GEG+ YS +D + A+I+ Sbjct: 211 ALKIILGHMGEGLPYSMWRIDHRNAWIKT 239 >3BVQ-A nrPDB Length = 383 Score = 26.2 bits (56), Expect = 4.7, Method: Compositional matrix adjust. Identities = 22/80 (27%), Positives = 36/80 (45%), Gaps = 4/80 (5%) Query: 3 KKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSF--LHV 60 K+TF + Y K +++ + AI Q W+ WL + + G+G +S Sbjct: 236 KRTFYQMAY-KFALSGHQRCAGTGFAIPQSVWKSWLRHLANPTLIDNGDGT-FSLGDTRN 293 Query: 61 DDKLAYIRVNHADKDTFSSS 80 D + A+I V D DT +S Sbjct: 294 DSENAWIFVFELDPDTDASP 313 >4HZV-A nrPDB Length = 388 Score = 25.0 bits (53), Expect = 9.2, Method: Compositional matrix adjust. Identities = 8/24 (33%), Positives = 15/24 (62%) Query: 110 EVTDDDRLWLKKVMEEEEQDCKCI 133 +TD + W K ++ +E +C+CI Sbjct: 130 RMTDSIKSWRKDILRTQESECQCI 153 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AH3-H nrPDB 271 6e-74 Sequences not found previously or not previously below threshold: 5GM5-A nrPDB 32 0.12 2VA8-A nrPDB 29 0.46 3X0V-A nrPDB 29 0.70 2QGM-A nrPDB 29 0.70 1J0H-A nrPDB 29 0.91 4HZV-A nrPDB 27 2.3 3FOT-A nrPDB 27 2.5 2X7Q-A nrPDB 27 2.6 3BVQ-A nrPDB 27 3.1 4NHM-A nrPDB 26 3.9 2I1J-A nrPDB 26 4.0 4MJ2-A nrPDB 26 4.0 6MWX-B nrPDB 25 6.7 6MUI-B nrPDB 25 6.7 5JDB-A nrPDB 25 8.1 5VX4-A nrPDB 25 9.7 CONVERGED! >6AH3-H nrPDB Length = 133 Score = 271 bits (693), Expect = 6e-74, Method: Composition-based stats. Identities = 133/133 (100%), Positives = 133/133 (100%) Query: 1 MGKKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLHV 60 MGKKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLHV Sbjct: 1 MGKKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLHV 60 Query: 61 DDKLAYIRVNHADKDTFSSSISTYISTDELVGSPLTVSILQESSSLRLLEVTDDDRLWLK 120 DDKLAYIRVNHADKDTFSSSISTYISTDELVGSPLTVSILQESSSLRLLEVTDDDRLWLK Sbjct: 61 DDKLAYIRVNHADKDTFSSSISTYISTDELVGSPLTVSILQESSSLRLLEVTDDDRLWLK 120 Query: 121 KVMEEEEQDCKCI 133 KVMEEEEQDCKCI Sbjct: 121 KVMEEEEQDCKCI 133 >5GM5-A nrPDB Length = 220 Score = 31.6 bits (70), Expect = 0.12, Method: Composition-based stats. Identities = 23/89 (25%), Positives = 34/89 (38%), Gaps = 18/89 (20%) Query: 29 IDQMTWRQWLNNALKRSYGIFGEGVEYSFL----------HVDDKLAYIRVNHADKDTFS 78 +D TW W YG G YSF+ V+D Y+ NH + Sbjct: 140 VDGQTWELW--------YGANGSQKTYSFVAPTPITSFQGDVNDFFKYLTQNHGFPASSQ 191 Query: 79 SSISTYISTDELVGSPLTVSILQESSSLR 107 I+ T G P T+S+ S+S++ Sbjct: 192 YLITLQFGTAPFTGGPATLSVSNWSASVQ 220 >2VA8-A nrPDB Length = 715 Score = 29.3 bits (64), Expect = 0.46, Method: Composition-based stats. Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 18/52 (34%) Query: 5 TFREWQY--FKLSITSFDQDVDDA------------HAIDQMTWR---QWLN 39 TF++W+ FK+++TS D D DDA +D + WR +WLN Sbjct: 94 TFKDWELIGFKVAMTSGDYDTDDAWLKNYDIIITTYEKLDSL-WRHRPEWLN 144 >3X0V-A nrPDB Length = 541 Score = 28.9 bits (63), Expect = 0.70, Method: Composition-based stats. Identities = 19/61 (31%), Positives = 32/61 (52%), Gaps = 6/61 (9%) Query: 1 MGKKTFREWQYFKLSITS-FDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLH 59 +G+K + + Y+K + FD+ D+ +D MT R++ LKR G GE +Y F Sbjct: 134 VGEKLQQAFGYYKEKLAEDFDKGFDELMLVDDMTTREY----LKRG-GPKGEAPKYDFFA 188 Query: 60 V 60 + Sbjct: 189 I 189 >2QGM-A nrPDB Length = 445 Score = 28.9 bits (63), Expect = 0.70, Method: Composition-based stats. Identities = 13/39 (33%), Positives = 22/39 (56%) Query: 47 GIFGEGVEYSFLHVDDKLAYIRVNHADKDTFSSSISTYI 85 G F + ++Y LH D LA + N+ + +F+ SI Y+ Sbjct: 178 GSFNKVIDYVRLHRPDLLAEVEENYKELSSFTGSIQEYM 216 >1J0H-A nrPDB Length = 588 Score = 28.5 bits (62), Expect = 0.91, Method: Composition-based stats. Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Query: 41 ALKRSYGIFGEGVEYSFLHVDDKLAYIRVNHADKD 75 AL++ Y G E SFLH DD++ Y+ D D Sbjct: 495 ALRKQYRSLRRG-EISFLHADDEMNYLIYKKTDGD 528 >4HZV-A nrPDB Length = 388 Score = 27.0 bits (58), Expect = 2.3, Method: Composition-based stats. Identities = 8/23 (34%), Positives = 15/23 (65%) Query: 111 VTDDDRLWLKKVMEEEEQDCKCI 133 +TD + W K ++ +E +C+CI Sbjct: 131 MTDSIKSWRKDILRTQESECQCI 153 >3FOT-A nrPDB Length = 519 Score = 27.0 bits (58), Expect = 2.5, Method: Composition-based stats. Identities = 10/27 (37%), Positives = 16/27 (59%) Query: 33 TWRQWLNNALKRSYGIFGEGVEYSFLH 59 ++RQWL N ++ G+ E S+LH Sbjct: 396 SYRQWLENPFLQALGLRVHNFEASYLH 422 >2X7Q-A nrPDB Length = 321 Score = 27.0 bits (58), Expect = 2.6, Method: Composition-based stats. Identities = 19/75 (25%), Positives = 32/75 (42%), Gaps = 16/75 (21%) Query: 9 WQYFKLSITSFDQDVDDAHAIDQM--TWRQWL-----------NNALKRSYGIFGEGVEY 55 W+YF S +D + IDQ+ W W+ ++ +K +G++Y Sbjct: 176 WEYFT-SKKYYDNH--EIKQIDQIYTPWSSWVVATSSDSLQAKSDVIKNFIDAVNQGIQY 232 Query: 56 SFLHVDDKLAYIRVN 70 HVD+ + YI N Sbjct: 233 YNEHVDEAIEYISSN 247 >3BVQ-A nrPDB Length = 383 Score = 26.6 bits (57), Expect = 3.1, Method: Composition-based stats. Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 4/79 (5%) Query: 3 KKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSF--LHV 60 K+TF + Y K +++ + AI Q W+ WL + + G+G +S Sbjct: 236 KRTFYQMAY-KFALSGHQRCAGTGFAIPQSVWKSWLRHLANPTLIDNGDGT-FSLGDTRN 293 Query: 61 DDKLAYIRVNHADKDTFSS 79 D + A+I V D DT +S Sbjct: 294 DSENAWIFVFELDPDTDAS 312 >4NHM-A nrPDB Length = 647 Score = 26.2 bits (56), Expect = 3.9, Method: Composition-based stats. Identities = 20/57 (35%), Positives = 34/57 (59%), Gaps = 7/57 (12%) Query: 81 ISTYISTDELVGSPLTVSILQ----ESSSLRLLEVTDDDRL-WLKKVMEEEEQDCKC 132 +S YIS + L S + LQ E+SSL++ +DD+ LKKV++++E + +C Sbjct: 341 LSQYISPEHL--SSKGIEKLQKQFVENSSLQIESFLNDDKSELLKKVIKQKELEQEC 395 >2I1J-A nrPDB Length = 575 Score = 26.2 bits (56), Expect = 4.0, Method: Composition-based stats. Identities = 13/51 (25%), Positives = 21/51 (41%) Query: 21 QDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLHVDDKLAYIRVNH 71 Q V D H + + W Q + N + G+ E +L + L VN+ Sbjct: 155 QRVTDQHKMSREEWEQSITNWWQEHRGMLREDAMMEYLKIAQDLEMYGVNY 205 >4MJ2-A nrPDB Length = 653 Score = 26.2 bits (56), Expect = 4.0, Method: Composition-based stats. Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 7/62 (11%) Query: 16 ITSFDQDVDDAH--AIDQMTWRQ----WLNNALKRSYGIFGEGVEYSFLHVDDKLAYIRV 69 + S+DQ ++ A+ A+ +Q WL L + G G+G+ Y+F H+D L +R Sbjct: 65 VLSWDQQLNLAYVGAVPHRGIKQVRTHWLLE-LVTTRGSTGQGLSYNFTHLDGYLDLLRE 123 Query: 70 NH 71 N Sbjct: 124 NQ 125 >6MWX-B nrPDB Length = 420 Score = 25.4 bits (54), Expect = 6.7, Method: Composition-based stats. Identities = 8/23 (34%), Positives = 15/23 (65%) Query: 35 RQWLNNALKRSYGIFGEGVEYSF 57 RQW+ ++ + GEG+EY++ Sbjct: 305 RQWIERPTTVNFTVTGEGLEYTW 327 >6MUI-B nrPDB Length = 477 Score = 25.4 bits (54), Expect = 6.7, Method: Composition-based stats. Identities = 8/23 (34%), Positives = 15/23 (65%) Query: 35 RQWLNNALKRSYGIFGEGVEYSF 57 RQW+ ++ + GEG+EY++ Sbjct: 305 RQWIERPTTVNFTVTGEGLEYTW 327 >5JDB-A nrPDB Length = 160 Score = 25.4 bits (54), Expect = 8.1, Method: Composition-based stats. Identities = 13/53 (24%), Positives = 25/53 (47%) Query: 40 NALKRSYGIFGEGVEYSFLHVDDKLAYIRVNHADKDTFSSSISTYISTDELVG 92 N + R Y +FGE +++ + DK ++ + + + T S +LVG Sbjct: 50 NPVDRQYTVFGENKQFNVRNDSDKWKFLEMFRSSSQNEFYNRRTLTSDTKLVG 102 >5VX4-A nrPDB Length = 162 Score = 25.0 bits (53), Expect = 9.7, Method: Composition-based stats. Identities = 13/49 (26%), Positives = 23/49 (46%) Query: 44 RSYGIFGEGVEYSFLHVDDKLAYIRVNHADKDTFSSSISTYISTDELVG 92 R Y +FGE +++ + DK + + + S+ T S + LVG Sbjct: 56 RQYVLFGENKQFNVENSSDKWKFFEMFKGSSQSDFSNRRTLTSNNRLVG 104 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.133 0.397 Lambda K H 0.267 0.0434 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,989,439 Number of Sequences: 36641 Number of extensions: 241131 Number of successful extensions: 616 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 596 Number of HSP's gapped (non-prelim): 34 length of query: 133 length of database: 10,336,785 effective HSP length: 86 effective length of query: 47 effective length of database: 7,185,659 effective search space: 337725973 effective search space used: 337725973 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.3 bits)