BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P37692 (348 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1PSW-A nrPDB 717 0.0 3TOV-A nrPDB 83 2e-16 2H1F-A nrPDB 52 4e-07 1SNY-A nrPDB 33 0.18 5FM1-B nrPDB 31 0.77 1SPG-B nrPDB 30 1.2 3C3W-A nrPDB 29 3.8 1Z47-A nrPDB 28 8.4 3U33-A nrPDB 27 9.0 >1PSW-A nrPDB Length = 348 Score = 717 bits (1852), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/348 (99%), Positives = 347/348 (99%) Query: 1 MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG 60 MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG Sbjct: 1 MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG 60 Query: 61 HGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGIPHRTGWRGEMRYGLLNDV 120 HGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVP FAGIPHRTGWRGEMRYGLLNDV Sbjct: 61 HGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPLFAGIPHRTGWRGEMRYGLLNDV 120 Query: 121 RVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQLQVSEGEKSYTCNQFSLSSER 180 RVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQLQVSEGEKSYTCNQFSLSSER Sbjct: 121 RVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQLQVSEGEKSYTCNQFSLSSER 180 Query: 181 PMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQ 240 PMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQ Sbjct: 181 PMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQ 240 Query: 241 AWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFTPPLSH 300 AWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFTPPLSH Sbjct: 241 AWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFTPPLSH 300 Query: 301 KARVIRLITGYHKVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEEA 348 KARVIRLITGYHKVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEEA Sbjct: 301 KARVIRLITGYHKVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEEA 348 >3TOV-A nrPDB Length = 349 Score = 83.2 bits (204), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 56/174 (32%), Positives = 84/174 (48%), Gaps = 7/174 (4%) Query: 176 LSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAAL 235 L+ +IGF G+ P KRWP +A +A GY+ V FG D E ++ + Sbjct: 181 LTDTDILIGFNIGSAV-PEKRWPAERFAHVADYFGRLGYKTVFFGGPMDLEMVQPVVEQM 239 Query: 236 NTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFT 295 T+ G+ QL + C ++TNDSG MHV + P+VALYGPS+P F Sbjct: 240 ETKPIV----ATGKFQLGPLAAAMNRCNLLITNDSGPMHVGISQGVPIVALYGPSNPFFY 295 Query: 296 PPLSHKARVIRLITGYH--KVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEE 347 P A V+ + Y K K EG ++ L I+ ++V++ LLL+ + Sbjct: 296 GPYQAHAIVLETMDSYEIGKSMKKIIKEGNYKGLSVISEEQVIKAAETLLLESK 349 >2H1F-A nrPDB Length = 334 Score = 52.0 bits (123), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 34/98 (34%), Positives = 51/98 (52%), Gaps = 5/98 (5%) Query: 195 KRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQ 254 K WP H+ EL L D G ++ L A E + LA E A+ L + L+ Sbjct: 192 KHWPEEHWRELIGLLADSGIRIKLPWGAPHEEERAKRLA----EGFAYVEVLP-KMSLEG 246 Query: 255 AVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSP 292 ++A K +V+ D+GL H+ AAL+RP + +YGP+ P Sbjct: 247 VARVLAGAKFVVSVDTGLSHLTAALDRPNITVYGPTDP 284 >1SNY-A nrPDB Length = 267 Score = 33.1 bits (74), Expect = 0.18, Method: Compositional matrix adjust. Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 3/48 (6%) Query: 17 SQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLGHGAL 64 SQ L TLQ ++ +M C PLL + + NE+ PM +G A+ Sbjct: 124 SQELLDTLQT---NTVVPIMLAKACLPLLKKAAKANESQPMGVGRAAI 168 >5FM1-B nrPDB Length = 846 Score = 31.2 bits (69), Expect = 0.77, Method: Compositional matrix adjust. Identities = 13/38 (34%), Positives = 21/38 (55%) Query: 200 YHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNT 237 Y + ++ L D GY++ + + DHEA N +L NT Sbjct: 770 YEFVKVYCNLNDIGYEIFIKMNLNDHEASNGLLGKFNT 807 >1SPG-B nrPDB Length = 147 Score = 30.4 bits (67), Expect = 1.2, Method: Compositional matrix adjust. Identities = 20/63 (31%), Positives = 31/63 (49%), Gaps = 4/63 (6%) Query: 143 GIMRTAQDLPQ-PLLWPQLQVSEGEKSYTC-NQFSLSSERPMIGFCPGAEFGPAKRWPHY 200 G+ R Q++ ++ QL + EK + + F L E +I C GA+FGP+ P Sbjct: 70 GLDRAVQNMDNIKNVYKQLSIKHSEKIHVDPDNFRLLGE--IITMCVGAKFGPSAFTPEI 127 Query: 201 HYA 203 H A Sbjct: 128 HEA 130 >3C3W-A nrPDB Length = 225 Score = 28.9 bits (63), Expect = 3.8, Method: Compositional matrix adjust. Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 5/41 (12%) Query: 16 MSQSLYRTLQARYPQAIIDVMAP-----AWCRPLLSRMPEV 51 +++++ R AR A++DV P CR LLSRMP++ Sbjct: 36 VAEAMARVPAARPDVAVLDVRLPDGNGIELCRDLLSRMPDL 76 >1Z47-A nrPDB Length = 355 Score = 27.7 bits (60), Expect = 8.4, Method: Compositional matrix adjust. Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 8/52 (15%) Query: 283 LVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR 334 +V L GPS S K ++RLI G + KGD G + + D+ PQ+ Sbjct: 43 MVGLLGPSG-------SGKTTILRLIAGLERPTKGDVWIG-GKRVTDLPPQK 86 >3U33-A nrPDB Length = 541 Score = 27.3 bits (59), Expect = 9.0, Method: Compositional matrix adjust. Identities = 18/70 (25%), Positives = 35/70 (50%), Gaps = 7/70 (10%) Query: 92 KSALVPFFAGIPHRTGWRGEMRYGLLNDVRVLDKEA--WPLMVERYIALA-----YDKGI 144 +S L + +P + W G L+ +RVL+K+A + L+ E ++ + +D+ + Sbjct: 408 ESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAV 467 Query: 145 MRTAQDLPQP 154 R Q L +P Sbjct: 468 RRLQQQLRKP 477 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1PSW-A nrPDB 655 0.0 3TOV-A nrPDB 247 7e-66 2H1F-A nrPDB 135 2e-32 Sequences not found previously or not previously below threshold: 4RAP-A nrPDB 37 0.011 1SNY-A nrPDB 35 0.054 1ZQ1-A nrPDB 30 1.5 3U33-A nrPDB 30 1.9 3C3W-A nrPDB 29 2.4 1UOU-A nrPDB 29 3.5 2M6S-A nrPDB 28 6.0 3L6X-A nrPDB 28 7.7 CONVERGED! >1PSW-A nrPDB Length = 348 Score = 655 bits (1691), Expect = 0.0, Method: Composition-based stats. Identities = 347/348 (99%), Positives = 347/348 (99%) Query: 1 MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG 60 MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG Sbjct: 1 MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG 60 Query: 61 HGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGIPHRTGWRGEMRYGLLNDV 120 HGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVP FAGIPHRTGWRGEMRYGLLNDV Sbjct: 61 HGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPLFAGIPHRTGWRGEMRYGLLNDV 120 Query: 121 RVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQLQVSEGEKSYTCNQFSLSSER 180 RVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQLQVSEGEKSYTCNQFSLSSER Sbjct: 121 RVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQLQVSEGEKSYTCNQFSLSSER 180 Query: 181 PMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQ 240 PMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQ Sbjct: 181 PMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQ 240 Query: 241 AWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFTPPLSH 300 AWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFTPPLSH Sbjct: 241 AWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFTPPLSH 300 Query: 301 KARVIRLITGYHKVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEEA 348 KARVIRLITGYHKVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEEA Sbjct: 301 KARVIRLITGYHKVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEEA 348 >3TOV-A nrPDB Length = 349 Score = 247 bits (630), Expect = 7e-66, Method: Composition-based stats. Identities = 56/174 (32%), Positives = 84/174 (48%), Gaps = 7/174 (4%) Query: 176 LSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAAL 235 L+ +IGF G+ P KRWP +A +A GY+ V FG D E ++ + Sbjct: 181 LTDTDILIGFNIGSAV-PEKRWPAERFAHVADYFGRLGYKTVFFGGPMDLEMVQPVVEQM 239 Query: 236 NTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFT 295 T+ G+ QL + C ++TNDSG MHV + P+VALYGPS+P F Sbjct: 240 ETKPIV----ATGKFQLGPLAAAMNRCNLLITNDSGPMHVGISQGVPIVALYGPSNPFFY 295 Query: 296 PPLSHKARVIRLITGYH--KVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEE 347 P A V+ + Y K K EG ++ L I+ ++V++ LLL+ + Sbjct: 296 GPYQAHAIVLETMDSYEIGKSMKKIIKEGNYKGLSVISEEQVIKAAETLLLESK 349 >2H1F-A nrPDB Length = 334 Score = 135 bits (341), Expect = 2e-32, Method: Composition-based stats. Identities = 79/318 (24%), Positives = 126/318 (39%), Gaps = 32/318 (10%) Query: 1 MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG 60 M++L++ S +GD++ + Q P D + + S V IP+ + Sbjct: 1 MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQIPSWHAAVERVIPVAIR 60 Query: 61 H------GALEIGERRKLGHSLREKRYDRAYVLPNSFKSA-LVPFFA-GIPHRTGWRGEM 112 A ER+ +L+ K YD KSA LV A G+ H W+ Sbjct: 61 RWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAALVTRLAHGVKHGMDWQ-TA 119 Query: 113 RYGLLNDVRVLDKEAWPLMVERYIALAYDK-GIMRTAQDLPQPLLWPQLQVSEGEKSYTC 171 R L + L R +A + + RT + + L + + Q ++G+ Y Sbjct: 120 REPLAS-----------LFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQ-TQGD--YAI 165 Query: 172 NQFSLSSERPMIG---FCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAG 228 Q L++ G A K WP H+ EL L D G ++ L A E Sbjct: 166 AQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWGAPHEEER 225 Query: 229 NEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYG 288 + LA E A+ L + L+ ++A K +V+ D+GL H+ AAL+RP + +YG Sbjct: 226 AKRLA----EGFAYVEVLP-KMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNITVYG 280 Query: 289 PSSPDFTPPLSHKARVIR 306 P+ P V R Sbjct: 281 PTDPGLIGGYGKNQMVCR 298 >4RAP-A nrPDB Length = 406 Score = 37.3 bits (85), Expect = 0.011, Method: Composition-based stats. Identities = 25/113 (22%), Positives = 43/113 (38%), Gaps = 21/113 (18%) Query: 194 AKRWPH-YHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQAW------CRNL 246 AK W + ++E+ L GY+V+ + + W + Sbjct: 229 AKYWNNGTGWSEVIAHLKSLGYRVMCIDRDAHY-----------GQGFVWNHIPWGAEDF 277 Query: 247 AGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYG---PSSPDFTP 296 G+ L + V L+ + SGL +A A P+V + G P+S +TP Sbjct: 278 TGKLPLQERVNLLRHASFFIGLPSGLSWLAWATRIPVVLISGFSLPNSEFYTP 330 >1SNY-A nrPDB Length = 267 Score = 35.0 bits (79), Expect = 0.054, Method: Composition-based stats. Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 3/48 (6%) Query: 17 SQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLGHGAL 64 SQ L TLQ ++ +M C PLL + + NE+ PM +G A+ Sbjct: 124 SQELLDTLQT---NTVVPIMLAKACLPLLKKAAKANESQPMGVGRAAI 168 >1ZQ1-A nrPDB Length = 438 Score = 30.0 bits (66), Expect = 1.5, Method: Composition-based stats. Identities = 22/101 (21%), Positives = 39/101 (38%), Gaps = 8/101 (7%) Query: 146 RTAQDLPQPLLWPQLQVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAEL 205 R+ D+P +WP ++ K Y S E + + K +P +E+ Sbjct: 266 RSINDVPIAKIWPNGEIEFLRKDYR----KRSDEEVEVDDKIEEKVALVKVYPGIS-SEI 320 Query: 206 AKQLIDEGYQ-VVLFGSAKDH--EAGNEILAALNTEQQAWC 243 L+D+GY+ +V+ G+ H + E A C Sbjct: 321 IDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVC 361 >3U33-A nrPDB Length = 541 Score = 29.6 bits (65), Expect = 1.9, Method: Composition-based stats. Identities = 18/70 (25%), Positives = 35/70 (50%), Gaps = 7/70 (10%) Query: 92 KSALVPFFAGIPHRTGWRGEMRYGLLNDVRVLDKEA--WPLMVERYIALA-----YDKGI 144 +S L + +P + W G L+ +RVL+K+A + L+ E ++ + +D+ + Sbjct: 408 ESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAV 467 Query: 145 MRTAQDLPQP 154 R Q L +P Sbjct: 468 RRLQQQLRKP 477 >3C3W-A nrPDB Length = 225 Score = 29.2 bits (64), Expect = 2.4, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 5/41 (12%) Query: 16 MSQSLYRTLQARYPQAIIDVMAP-----AWCRPLLSRMPEV 51 +++++ R AR A++DV P CR LLSRMP++ Sbjct: 36 VAEAMARVPAARPDVAVLDVRLPDGNGIELCRDLLSRMPDL 76 >1UOU-A nrPDB Length = 474 Score = 28.9 bits (63), Expect = 3.5, Method: Composition-based stats. Identities = 18/84 (21%), Positives = 28/84 (33%) Query: 188 GAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQAWCRNLA 247 GA P + L G +V +A D G + AL E+ C + A Sbjct: 232 GAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGA 291 Query: 248 GETQLDQAVILIAACKAIVTNDSG 271 G L V + ++ +G Sbjct: 292 GPPDLRDLVTTLGGALLWLSGHAG 315 >2M6S-A nrPDB Length = 149 Score = 28.1 bits (61), Expect = 6.0, Method: Composition-based stats. Identities = 12/31 (38%), Positives = 19/31 (61%), Gaps = 4/31 (12%) Query: 87 LPNSFKSALVPFFAGIPHRTGWRGEMRYGLL 117 LP+S +VP F GI G++ +RYG++ Sbjct: 64 LPDS----IVPLFQGIKDSLGFQPNLRYGVI 90 >3L6X-A nrPDB Length = 584 Score = 27.7 bits (60), Expect = 7.7, Method: Composition-based stats. Identities = 15/84 (17%), Positives = 31/84 (36%), Gaps = 1/84 (1%) Query: 244 RNLAGETQLDQAVILIAACKAIVT-NDSGLMHVAAALNRPLVALYGPSSPDFTPPLSHKA 302 +N + D + ++ ++ N + + L S + A Sbjct: 409 QNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAA 468 Query: 303 RVIRLITGYHKVRKGDAAEGYHQS 326 V++ I GY ++RK EG+ +S Sbjct: 469 LVLQTIWGYKELRKPLEKEGWKKS 492 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.136 0.406 Lambda K H 0.267 0.0393 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,148,507 Number of Sequences: 36641 Number of extensions: 603774 Number of successful extensions: 1370 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 1352 Number of HSP's gapped (non-prelim): 27 length of query: 348 length of database: 10,336,785 effective HSP length: 98 effective length of query: 250 effective length of database: 6,745,967 effective search space: 1686491750 effective search space used: 1686491750 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 59 (27.4 bits)