BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P36149 (193 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3CUE-D nrPDB 399 e-112 1SZ7-A nrPDB 203 3e-53 2C0J-A nrPDB 192 1e-49 6AQ3-A nrPDB 167 2e-42 6J2C-O nrPDB 30 0.66 4NAR-A nrPDB 28 3.1 4V6U-AA nrPDB 27 5.3 >3CUE-D nrPDB Length = 193 Score = 399 bits (1026), Expect = e-112, Method: Compositional matrix adjust. Identities = 193/193 (100%), Positives = 193/193 (100%) Query: 1 MVSTTQSRSLKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMG 60 MVSTTQSRSLKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMG Sbjct: 1 MVSTTQSRSLKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMG 60 Query: 61 YNIGCRLIEDFLARTALPRCENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILD 120 YNIGCRLIEDFLARTALPRCENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILD Sbjct: 61 YNIGCRLIEDFLARTALPRCENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILD 120 Query: 121 ENPLADFVELPMDAMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKL 180 ENPLADFVELPMDAMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKL Sbjct: 121 ENPLADFVELPMDAMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKL 180 Query: 181 NRILKDEIPIGED 193 NRILKDEIPIGED Sbjct: 181 NRILKDEIPIGED 193 >1SZ7-A nrPDB Length = 200 Score = 203 bits (517), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 97/173 (56%), Positives = 132/173 (76%), Gaps = 2/173 (1%) Query: 21 KTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIEDFLARTALPRC 80 +++K+++ELFTLTYG++V QLC+DYE D VN L MG+NIG RLIEDFLAR+ + RC Sbjct: 19 ESKKMSSELFTLTYGALVTQLCKDYEND-EDVNKQLDKMGFNIGVRLIEDFLARSNVGRC 77 Query: 81 ENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILDENPLADFVELPMDAMKSLWY 140 + +T++V++K AFK++L ITP+ITNWS D FSLIL+ NPL DFVELP D SL Y Sbjct: 78 HDFRETADVIAKVAFKMYLGITPSITNWSPAGDEFSLILENNPLVDFVELP-DNHSSLIY 136 Query: 141 SNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKLNRILKDEIPIGED 193 SN+LCGVL+G+LEMVQ+ + FV D L+GD TEI+++ R ++D +P GE+ Sbjct: 137 SNLLCGVLRGALEMVQMAVEAKFVQDTLKGDGVTEIRMRFIRRIEDNLPAGEE 189 >2C0J-A nrPDB Length = 161 Score = 192 bits (487), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 93/163 (57%), Positives = 123/163 (75%), Gaps = 2/163 (1%) Query: 26 NTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIEDFLARTALPRCENLVK 85 ++ELFTLTYG++V QLC+DYE D VN L MGYNIG RLIEDFLAR+ + RC + + Sbjct: 1 SSELFTLTYGALVTQLCKDYEND-EDVNKQLDRMGYNIGVRLIEDFLARSNVGRCHDFRE 59 Query: 86 TSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILDENPLADFVELPMDAMKSLWYSNILC 145 T++V++K AFK++L ITP+ITNWS D FSLIL+ NPL DFVELP D +L YSN+LC Sbjct: 60 TADVIAKVAFKMYLGITPSITNWSPAGDEFSLILENNPLVDFVELP-DNHSALIYSNLLC 118 Query: 146 GVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKLNRILKDEI 188 GVL+G+LEMVQ+ + FV D L+GD TEI+++ R ++D + Sbjct: 119 GVLRGALEMVQMAVEAKFVQDTLKGDGVTEIRMRFIRRIEDNL 161 >6AQ3-A nrPDB Length = 195 Score = 167 bits (424), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 83/183 (45%), Positives = 122/183 (66%), Gaps = 5/183 (2%) Query: 10 LKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIE 69 L +GE + NK EKIN+EL +TYGS+V Q+ +DYE D +N L MGY +G RLI+ Sbjct: 12 LAKIGETTF-NKIEKINSELLAMTYGSLVTQMLKDYE-DVAAINTQLEKMGYKMGMRLID 69 Query: 70 DFLARTALPR--CENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILDENPLADF 127 +F++++ L C T+E ++K AFK+FL I N+TNWS ++ +S++ DENPL DF Sbjct: 70 EFMSKSGLSSGACREFKDTAESIAKVAFKMFLGINANVTNWSKDQTEYSIVFDENPLNDF 129 Query: 128 VELPMD-AMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKLNRILKD 186 VELP K L+YSNI+CGV++G+LEMV + + + L GD Q+EI+V+L L++ Sbjct: 130 VELPEPIKQKRLYYSNIICGVIRGALEMVLMRVECEYKKCPLLGDDQSEIRVRLKEYLRE 189 Query: 187 EIP 189 +P Sbjct: 190 TVP 192 >6J2C-O nrPDB Length = 393 Score = 30.0 bits (66), Expect = 0.66, Method: Compositional matrix adjust. Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 1/53 (1%) Query: 129 ELPMDAMKSLWYSNILCGVLKGSLEMV-QLDCDVWFVSDILRGDSQTEIKVKL 180 LP D ++ L I G+LKGS++ V +L W I+ GD T++K +L Sbjct: 318 HLPKDNVEHLVMRAISLGLLKGSIDQVNELVTISWVQPRIISGDQITKMKDRL 370 >4NAR-A nrPDB Length = 435 Score = 27.7 bits (60), Expect = 3.1, Method: Compositional matrix adjust. Identities = 11/22 (50%), Positives = 17/22 (77%) Query: 163 FVSDILRGDSQTEIKVKLNRIL 184 FVS++ GD + EI +K+NR+L Sbjct: 117 FVSEMTEGDLEEEIPIKVNRLL 138 >4V6U-AA nrPDB Length = 198 Score = 26.9 bits (58), Expect = 5.3, Method: Compositional matrix adjust. Identities = 13/50 (26%), Positives = 30/50 (60%), Gaps = 2/50 (4%) Query: 2 VSTTQSRSLKAMGEEIWKNKTEKINTELFTL--TYGSIVAQLCQDYERDF 49 + T+Q R+++ + +EI K E++N + F L G I A++ ++ ++ + Sbjct: 129 IQTSQERAIRKIMQEIIYKKAEELNFKDFVLESVNGKIAAEIAKEAKKIY 178 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3CUE-D nrPDB 325 9e-90 1SZ7-A nrPDB 295 1e-80 2C0J-A nrPDB 277 2e-75 6AQ3-A nrPDB 265 9e-72 Sequences not found previously or not previously below threshold: 2J3W-B nrPDB 35 0.014 2J3R-B nrPDB 33 0.088 6J2C-O nrPDB 31 0.30 3DLS-A nrPDB 30 0.48 2CFH-C nrPDB 29 1.7 3CUE-B nrPDB 28 2.1 CONVERGED! >3CUE-D nrPDB Length = 193 Score = 325 bits (832), Expect = 9e-90, Method: Composition-based stats. Identities = 193/193 (100%), Positives = 193/193 (100%) Query: 1 MVSTTQSRSLKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMG 60 MVSTTQSRSLKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMG Sbjct: 1 MVSTTQSRSLKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMG 60 Query: 61 YNIGCRLIEDFLARTALPRCENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILD 120 YNIGCRLIEDFLARTALPRCENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILD Sbjct: 61 YNIGCRLIEDFLARTALPRCENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILD 120 Query: 121 ENPLADFVELPMDAMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKL 180 ENPLADFVELPMDAMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKL Sbjct: 121 ENPLADFVELPMDAMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKL 180 Query: 181 NRILKDEIPIGED 193 NRILKDEIPIGED Sbjct: 181 NRILKDEIPIGED 193 >1SZ7-A nrPDB Length = 200 Score = 295 bits (754), Expect = 1e-80, Method: Composition-based stats. Identities = 97/173 (56%), Positives = 132/173 (76%), Gaps = 2/173 (1%) Query: 21 KTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIEDFLARTALPRC 80 +++K+++ELFTLTYG++V QLC+DYE D VN L MG+NIG RLIEDFLAR+ + RC Sbjct: 19 ESKKMSSELFTLTYGALVTQLCKDYEND-EDVNKQLDKMGFNIGVRLIEDFLARSNVGRC 77 Query: 81 ENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILDENPLADFVELPMDAMKSLWY 140 + +T++V++K AFK++L ITP+ITNWS D FSLIL+ NPL DFVELP D SL Y Sbjct: 78 HDFRETADVIAKVAFKMYLGITPSITNWSPAGDEFSLILENNPLVDFVELP-DNHSSLIY 136 Query: 141 SNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKLNRILKDEIPIGED 193 SN+LCGVL+G+LEMVQ+ + FV D L+GD TEI+++ R ++D +P GE+ Sbjct: 137 SNLLCGVLRGALEMVQMAVEAKFVQDTLKGDGVTEIRMRFIRRIEDNLPAGEE 189 >2C0J-A nrPDB Length = 161 Score = 277 bits (709), Expect = 2e-75, Method: Composition-based stats. Identities = 93/163 (57%), Positives = 123/163 (75%), Gaps = 2/163 (1%) Query: 26 NTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIEDFLARTALPRCENLVK 85 ++ELFTLTYG++V QLC+DYE D VN L MGYNIG RLIEDFLAR+ + RC + + Sbjct: 1 SSELFTLTYGALVTQLCKDYEND-EDVNKQLDRMGYNIGVRLIEDFLARSNVGRCHDFRE 59 Query: 86 TSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILDENPLADFVELPMDAMKSLWYSNILC 145 T++V++K AFK++L ITP+ITNWS D FSLIL+ NPL DFVELP D +L YSN+LC Sbjct: 60 TADVIAKVAFKMYLGITPSITNWSPAGDEFSLILENNPLVDFVELP-DNHSALIYSNLLC 118 Query: 146 GVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKLNRILKDEI 188 GVL+G+LEMVQ+ + FV D L+GD TEI+++ R ++D + Sbjct: 119 GVLRGALEMVQMAVEAKFVQDTLKGDGVTEIRMRFIRRIEDNL 161 >6AQ3-A nrPDB Length = 195 Score = 265 bits (677), Expect = 9e-72, Method: Composition-based stats. Identities = 83/183 (45%), Positives = 122/183 (66%), Gaps = 5/183 (2%) Query: 10 LKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIE 69 L +GE + NK EKIN+EL +TYGS+V Q+ +DYE D +N L MGY +G RLI+ Sbjct: 12 LAKIGETTF-NKIEKINSELLAMTYGSLVTQMLKDYE-DVAAINTQLEKMGYKMGMRLID 69 Query: 70 DFLARTALPR--CENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILDENPLADF 127 +F++++ L C T+E ++K AFK+FL I N+TNWS ++ +S++ DENPL DF Sbjct: 70 EFMSKSGLSSGACREFKDTAESIAKVAFKMFLGINANVTNWSKDQTEYSIVFDENPLNDF 129 Query: 128 VELPMD-AMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKLNRILKD 186 VELP K L+YSNI+CGV++G+LEMV + + + L GD Q+EI+V+L L++ Sbjct: 130 VELPEPIKQKRLYYSNIICGVIRGALEMVLMRVECEYKKCPLLGDDQSEIRVRLKEYLRE 189 Query: 187 EIP 189 +P Sbjct: 190 TVP 192 >2J3W-B nrPDB Length = 188 Score = 35.4 bits (80), Expect = 0.014, Method: Composition-based stats. Identities = 27/148 (18%), Positives = 58/148 (39%), Gaps = 9/148 (6%) Query: 7 SRSLKAMGEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCR 66 +R A+ E +++ F L + +V Q CQ +++ L MG +G Sbjct: 6 TRGKSAILERSLTRPKTEVSVSAFALLFSEMV-QYCQSRVYSVSELQARLADMGQGVGAS 64 Query: 67 LIEDFLARTALPRCENLVKTSEVLSKC-AFKIFLNITPNITNWSHNKDTFSLILDENPLA 125 L++ + R + E V + K +K + +++ D I+++ PL Sbjct: 65 LLDVLVMREKNGKRETKVLNILLFIKVNVWKALFGKEADKLEQANDDDKTYYIIEKEPLI 124 Query: 126 D-FVELPMDAMKSLWYSNILCGVLKGSL 152 + ++ +P + S + C G + Sbjct: 125 NAYISVPKEN------STLNCAAFTGGI 146 >2J3R-B nrPDB Length = 157 Score = 33.1 bits (74), Expect = 0.088, Method: Composition-based stats. Identities = 24/131 (18%), Positives = 53/131 (40%), Gaps = 9/131 (6%) Query: 24 KINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIEDFLARTALPRCENL 83 +++ F L + +V Q CQ +++ L MG +G L++ + R + E Sbjct: 4 EVSVSAFALLFSEMV-QYCQSRVYSVSELQARLADMGQGVGASLLDVLVMREKNGKRETK 62 Query: 84 VKTSEVLSKC-AFKIFLNITPNITNWSHNKDTFSLILDENPLAD-FVELPMDAMKSLWYS 141 V + K +K + +++ D I+++ PL + ++ +P + S Sbjct: 63 VLNILLFIKVNVWKALFGKEADKLEQANDDDKTYYIIEKEPLINAYISVPKEN------S 116 Query: 142 NILCGVLKGSL 152 + C G + Sbjct: 117 TLNCAAFTGGI 127 >6J2C-O nrPDB Length = 393 Score = 31.1 bits (69), Expect = 0.30, Method: Composition-based stats. Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Query: 130 LPMDAMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSD-ILRGDSQTEIKVKLNR 182 LP D ++ L I G+LKGS++ V + +V I+ GD T++K +L Sbjct: 319 LPKDNVEHLVMRAISLGLLKGSIDQVNELVTISWVQPRIISGDQITKMKDRLVE 372 >3DLS-A nrPDB Length = 335 Score = 30.4 bits (67), Expect = 0.48, Method: Composition-based stats. Identities = 23/109 (21%), Positives = 42/109 (38%), Gaps = 5/109 (4%) Query: 51 KVNDHLYSMGYNIGCRLIEDFLARTALPRCENLVKTSEVLSKCAFKIFLN---ITPNITN 107 + D + + +LI DF + L R + + CA ++ + P + Sbjct: 155 DIKDENIVIAEDFTIKLI-DFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEM 213 Query: 108 WSHNKDTFSLILDENPLADFVELPMDA-MKSLWYSNILCGVLKGSLEMV 155 WS ++L+ +ENP + E A S L ++ G L+ V Sbjct: 214 WSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPV 262 >2CFH-C nrPDB Length = 158 Score = 28.8 bits (63), Expect = 1.7, Method: Composition-based stats. Identities = 22/130 (16%), Positives = 47/130 (36%), Gaps = 11/130 (8%) Query: 29 LFTLTYGSIVAQLCQDYER---DFNKVNDHLYSMGYNIGCRLIEDFLARTALPRCENLVK 85 LF L + +V+ + + E+ + + L +MG+ +G LIE F TA Sbjct: 6 LFLLLHNEMVSGVYKSAEQGEVENGRCITKLENMGFRVGQGLIERFTKDTA-----RFKD 60 Query: 86 TSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILDENPLADFVELPMDAM---KSLWYSN 142 +++ + + + +L +N ++ + Y Sbjct: 61 ELDIMKFICKDFWTTVFKKQIDNLRTNHQGIYVLQDNKFRLLTQMSAGKQYLEHASKYLA 120 Query: 143 ILCGVLKGSL 152 CG+++G L Sbjct: 121 FTCGLIRGGL 130 >3CUE-B nrPDB Length = 283 Score = 28.4 bits (62), Expect = 2.1, Method: Composition-based stats. Identities = 17/79 (21%), Positives = 35/79 (44%), Gaps = 6/79 (7%) Query: 21 KTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIEDF-----LART 75 K ++ + + +++QL + + L G+NIG RL+E ++ + Sbjct: 53 KRQEASLSAMAFLFQEMISQLHRTCKT-AGDFETKLSDYGHNIGIRLLELLNFRASVSPS 111 Query: 76 ALPRCENLVKTSEVLSKCA 94 +LPR + +E SK + Sbjct: 112 SLPRASAFLSQNESSSKLS 130 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.138 0.373 Lambda K H 0.267 0.0424 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,155,640 Number of Sequences: 36641 Number of extensions: 265519 Number of successful extensions: 599 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 572 Number of HSP's gapped (non-prelim): 24 length of query: 193 length of database: 10,336,785 effective HSP length: 92 effective length of query: 101 effective length of database: 6,965,813 effective search space: 703547113 effective search space used: 703547113 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 56 (26.1 bits)