BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P36121 (282 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5FJ9-M nrPDB 574 e-164 6CCI-A nrPDB 28 6.0 >5FJ9-M nrPDB Length = 282 Score = 574 bits (1480), Expect = e-164, Method: Compositional matrix adjust. Identities = 282/282 (100%), Positives = 282/282 (100%) Query: 1 MSIDNKLFVTEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDD 60 MSIDNKLFVTEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDD Sbjct: 1 MSIDNKLFVTEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDD 60 Query: 61 TGEEEEDDPVIEEFPLKISGEEESLHVFQYANRPRLVGRKPAEHPFISAARYKPKSHLWE 120 TGEEEEDDPVIEEFPLKISGEEESLHVFQYANRPRLVGRKPAEHPFISAARYKPKSHLWE Sbjct: 61 TGEEEEDDPVIEEFPLKISGEEESLHVFQYANRPRLVGRKPAEHPFISAARYKPKSHLWE 120 Query: 121 IDIPLDEQAFYNKDKAESEWNGVNVQTLKGVGVENNGQYAAFVKDMQVYLVPIERVAQLK 180 IDIPLDEQAFYNKDKAESEWNGVNVQTLKGVGVENNGQYAAFVKDMQVYLVPIERVAQLK Sbjct: 121 IDIPLDEQAFYNKDKAESEWNGVNVQTLKGVGVENNGQYAAFVKDMQVYLVPIERVAQLK 180 Query: 181 PFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEE 240 PFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEE Sbjct: 181 PFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEE 240 Query: 241 ANIELTWAEGTFEQFKDTIVKEAEDKTLVALEKQEDYIDNLV 282 ANIELTWAEGTFEQFKDTIVKEAEDKTLVALEKQEDYIDNLV Sbjct: 241 ANIELTWAEGTFEQFKDTIVKEAEDKTLVALEKQEDYIDNLV 282 >6CCI-A nrPDB Length = 487 Score = 27.7 bits (60), Expect = 6.0, Method: Compositional matrix adjust. Identities = 15/35 (42%), Positives = 19/35 (54%), Gaps = 2/35 (5%) Query: 216 VKSVNDPSQNRL--TGSLLAHKVADEEANIELTWA 248 V+SV P + L TGSL +V D A +E WA Sbjct: 229 VQSVVPPGRKSLNKTGSLSVFRVEDYNATVEFYWA 263 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5FJ9-M nrPDB 538 e-153 Sequences not found previously or not previously below threshold: 6FAI-k nrPDB 32 0.20 5WWN-A nrPDB 32 0.20 1FLA-A nrPDB 31 0.59 5OQL-c nrPDB 30 1.2 6NUW-C nrPDB 29 2.0 6CCI-A nrPDB 29 2.6 2WAD-A nrPDB 29 3.1 3WQT-A nrPDB 29 3.2 5GAD-l nrPDB 28 5.0 5U0S-f nrPDB 28 5.4 5OQM-W nrPDB 27 6.5 5FZ5-W nrPDB 27 6.5 4DNG-A nrPDB 27 8.3 CONVERGED! >5FJ9-M nrPDB Length = 282 Score = 538 bits (1385), Expect = e-153, Method: Composition-based stats. Identities = 282/282 (100%), Positives = 282/282 (100%) Query: 1 MSIDNKLFVTEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDD 60 MSIDNKLFVTEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDD Sbjct: 1 MSIDNKLFVTEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDD 60 Query: 61 TGEEEEDDPVIEEFPLKISGEEESLHVFQYANRPRLVGRKPAEHPFISAARYKPKSHLWE 120 TGEEEEDDPVIEEFPLKISGEEESLHVFQYANRPRLVGRKPAEHPFISAARYKPKSHLWE Sbjct: 61 TGEEEEDDPVIEEFPLKISGEEESLHVFQYANRPRLVGRKPAEHPFISAARYKPKSHLWE 120 Query: 121 IDIPLDEQAFYNKDKAESEWNGVNVQTLKGVGVENNGQYAAFVKDMQVYLVPIERVAQLK 180 IDIPLDEQAFYNKDKAESEWNGVNVQTLKGVGVENNGQYAAFVKDMQVYLVPIERVAQLK Sbjct: 121 IDIPLDEQAFYNKDKAESEWNGVNVQTLKGVGVENNGQYAAFVKDMQVYLVPIERVAQLK 180 Query: 181 PFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEE 240 PFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEE Sbjct: 181 PFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEE 240 Query: 241 ANIELTWAEGTFEQFKDTIVKEAEDKTLVALEKQEDYIDNLV 282 ANIELTWAEGTFEQFKDTIVKEAEDKTLVALEKQEDYIDNLV Sbjct: 241 ANIELTWAEGTFEQFKDTIVKEAEDKTLVALEKQEDYIDNLV 282 >6FAI-k nrPDB Length = 788 Score = 32.4 bits (72), Expect = 0.20, Method: Composition-based stats. Identities = 27/120 (22%), Positives = 53/120 (44%), Gaps = 29/120 (24%) Query: 45 ANEQEEKSEEVKAEDDTGEEEEDDPVIEEFPLKISGEEE-------------------SL 85 ANE+EE +E+ +D+T E +D+ ++E+ +++G+EE L Sbjct: 411 ANEEEE-AEQTNGKDETMMEIDDEMMVEQDNEEVAGDEEYDIEDNEGFEELSPEEEERQL 469 Query: 86 HVFQYANR-----PRLVGRKPAEHPFISAARYKPKSHLWEIDIPLDEQAFYNKDKAESEW 140 F+ + P + +P+E RY+ +L+ D +DE+ + +EW Sbjct: 470 REFRDMEKEDREFPDEIELEPSESAIERLKRYRGLKNLYNCDWQVDEK----DPSSPAEW 525 >5WWN-A nrPDB Length = 709 Score = 32.4 bits (72), Expect = 0.20, Method: Composition-based stats. Identities = 27/120 (22%), Positives = 53/120 (44%), Gaps = 29/120 (24%) Query: 45 ANEQEEKSEEVKAEDDTGEEEEDDPVIEEFPLKISGEEE-------------------SL 85 ANE+EE +E+ +D+T E +D+ ++E+ +++G+EE L Sbjct: 332 ANEEEE-AEQTNGKDETMMEIDDEMMVEQDNEEVAGDEEYDIEDNEGFEELSPEEEERQL 390 Query: 86 HVFQYANR-----PRLVGRKPAEHPFISAARYKPKSHLWEIDIPLDEQAFYNKDKAESEW 140 F+ + P + +P+E RY+ +L+ D +DE+ + +EW Sbjct: 391 REFRDMEKEDREFPDEIELEPSESAIERLKRYRGLKNLYNCDWQVDEK----DPSSPAEW 446 >1FLA-A nrPDB Length = 138 Score = 30.9 bits (68), Expect = 0.59, Method: Composition-based stats. Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 6/56 (10%) Query: 36 DENGTNSAIANEQEEKSEEV------KAEDDTGEEEEDDPVIEEFPLKISGEEESL 85 D N N + N E +E++ +D+ EE E +P IEE KISG++ +L Sbjct: 29 DVNTINVSDVNIDELLNEDILILGCSAMDDEVLEESEFEPFIEEISTKISGKKVAL 84 >5OQL-c nrPDB Length = 785 Score = 30.1 bits (66), Expect = 1.2, Method: Composition-based stats. Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 12/63 (19%) Query: 22 ADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDDTGEEEEDDPVIEEFPLKISGE 81 AD ED + + DEN +++AE+D+G EE+ V EE ++ GE Sbjct: 203 ADTEDSDGEGASLGDENAMF------------DLEAEEDSGSEEDKSDVGEEVDGEVDGE 250 Query: 82 EES 84 + S Sbjct: 251 KHS 253 Score = 27.0 bits (58), Expect = 8.3, Method: Composition-based stats. Identities = 16/37 (43%), Positives = 23/37 (62%), Gaps = 2/37 (5%) Query: 2 SID--NKLFVTEEDEEDRTQDRADVEDESNDIDMIAD 36 SID NK ED++ R + A+++D+S DID AD Sbjct: 294 SIDEFNKQTQMWEDQDMRAEPTAELDDDSEDIDWHAD 330 >6NUW-C nrPDB Length = 371 Score = 29.3 bits (64), Expect = 2.0, Method: Composition-based stats. Identities = 26/101 (25%), Positives = 45/101 (44%), Gaps = 18/101 (17%) Query: 24 VEDESNDIDMIADENGTNSAIANEQEEKSEEVKAE--DDTGEEEEDDPVIEEFPLKISGE 81 ++D DI+ + E N EE E++KA+ D EE +P+++EF + + Sbjct: 7 IDDLQQDIESLLSE-------INSLEESREKLKAKIKDKRKNEESANPIVQEFE-DLFDQ 58 Query: 82 EESLHVFQYANRPRL-------VGRKPAEHPFISAARYKPK 115 L+ F + P L + R A+ P + Y+PK Sbjct: 59 FPQLNNFLFNEHPELEETDDKDISRAQADIP-ATPIPYEPK 98 >6CCI-A nrPDB Length = 487 Score = 28.9 bits (63), Expect = 2.6, Method: Composition-based stats. Identities = 17/44 (38%), Positives = 21/44 (47%), Gaps = 2/44 (4%) Query: 216 VKSVNDPSQNRL--TGSLLAHKVADEEANIELTWAEGTFEQFKD 257 V+SV P + L TGSL +V D A +E WA E D Sbjct: 229 VQSVVPPGRKSLNKTGSLSVFRVEDYNATVEFYWAPFLVESNSD 272 >2WAD-A nrPDB Length = 680 Score = 28.6 bits (62), Expect = 3.1, Method: Composition-based stats. Identities = 25/129 (19%), Positives = 49/129 (37%), Gaps = 9/129 (6%) Query: 130 FYNKDKAESEWNGVNVQTLKGVGVENNG---------QYAAFVKDMQVYLVPIERVAQLK 180 FY K A + V + +G + +G Q +F + ++ ++ +A+ Sbjct: 41 FYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKEIAKKL 100 Query: 181 PFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEE 240 + I N+T +Q +P + V + + D NRL+ S L + D Sbjct: 101 LTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNNAVDSV 160 Query: 241 ANIELTWAE 249 +L + E Sbjct: 161 PTSQLNYTE 169 >3WQT-A nrPDB Length = 484 Score = 28.6 bits (62), Expect = 3.2, Method: Composition-based stats. Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 8/62 (12%) Query: 23 DVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDDTGEEEEDDPVIEEFPLKISGEE 82 +V DESN+ID D S I E+ E ++ ++D E E VI FP++ ++ Sbjct: 100 EVYDESNEIDFYEDTEINGSHI----EKVLEGIREKNDVQETE----VINVFPIRFIVDK 151 Query: 83 ES 84 E+ Sbjct: 152 EN 153 Score = 27.4 bits (59), Expect = 6.4, Method: Composition-based stats. Identities = 15/65 (23%), Positives = 27/65 (41%), Gaps = 5/65 (7%) Query: 9 VTEEDEEDRTQDRADVEDESND-----IDMIADENGTNSAIANEQEEKSEEVKAEDDTGE 63 + D E+ +D DV+D+ N+ D + NE E EE+ +D + Sbjct: 397 INYHDNEETEEDVIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESSDEEIYQSEDNHQ 456 Query: 64 EEEDD 68 E + + Sbjct: 457 EHKQN 461 >5GAD-l nrPDB Length = 497 Score = 27.8 bits (60), Expect = 5.0, Method: Composition-based stats. Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 11/76 (14%) Query: 10 TEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDDTGEEEEDDP 69 +E+ E++ Q + E E+ D++ EQ +SE+ + E + + E P Sbjct: 48 SEQAVEEQPQAHTEAEAETFAADVVE---------VTEQVAESEKAQPEAEVVAQPE--P 96 Query: 70 VIEEFPLKISGEEESL 85 V+EE P ++ E E L Sbjct: 97 VVEETPEPVAIEREEL 112 >5U0S-f nrPDB Length = 142 Score = 27.8 bits (60), Expect = 5.4, Method: Composition-based stats. Identities = 17/48 (35%), Positives = 27/48 (56%), Gaps = 1/48 (2%) Query: 12 EDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAED 59 E++E D A +E+E ++++MI DENG + + E S V ED Sbjct: 5 EEDEAFGMDGAVMEEEVDELEMI-DENGQSQQGVSHPGEPSTTVITED 51 >5OQM-W nrPDB Length = 541 Score = 27.4 bits (59), Expect = 6.5, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 23/47 (48%) Query: 13 DEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAED 59 D+ D T +EDE D+ A T S +N+ +++S K ED Sbjct: 457 DDNDETARENALEDEFEDVTDTAGTAKTESNTSNDVKQESINDKTED 503 >5FZ5-W nrPDB Length = 482 Score = 27.4 bits (59), Expect = 6.5, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 23/47 (48%) Query: 13 DEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAED 59 D+ D T +EDE D+ A T S +N+ +++S K ED Sbjct: 398 DDNDETARENALEDEFEDVTDTAGTAKTESNTSNDVKQESINDKTED 444 >4DNG-A nrPDB Length = 485 Score = 27.0 bits (58), Expect = 8.3, Method: Composition-based stats. Identities = 18/63 (28%), Positives = 30/63 (47%), Gaps = 1/63 (1%) Query: 31 IDMIAD-ENGTNSAIANEQEEKSEEVKAEDDTGEEEEDDPVIEEFPLKISGEEESLHVFQ 89 IDM D E G +SA+ EK E+ + D+G +D + + P G ++ V + Sbjct: 399 IDMANDTEYGLSSAVFTSDLEKGEKFALQIDSGMTHVNDQSVNDSPNIAFGGNKASGVGR 458 Query: 90 YAN 92 + N Sbjct: 459 FGN 461 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.308 0.128 0.353 Lambda K H 0.267 0.0398 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,493,840 Number of Sequences: 36641 Number of extensions: 495169 Number of successful extensions: 2017 Number of sequences better than 10.0: 55 Number of HSP's better than 10.0 without gapping: 58 Number of HSP's successfully gapped in prelim test: 68 Number of HSP's that attempted gapping in prelim test: 1442 Number of HSP's gapped (non-prelim): 385 length of query: 282 length of database: 10,336,785 effective HSP length: 96 effective length of query: 186 effective length of database: 6,819,249 effective search space: 1268380314 effective search space used: 1268380314 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 58 (27.0 bits)