BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P36068 (246 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5M0J-D nrPDB 486 e-138 1XLY-A nrPDB 466 e-132 2GAK-A nrPDB 29 2.0 6DKT-A nrPDB 28 2.5 2AU3-A nrPDB 28 3.2 4LDP-A nrPDB 28 3.7 2Z4T-A nrPDB 27 9.4 >5M0J-D nrPDB Length = 328 Score = 486 bits (1251), Expect = e-138, Method: Compositional matrix adjust. Identities = 237/241 (98%), Positives = 237/241 (98%) Query: 6 DIKVTPGTCELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY 65 DIKVTPGT ELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY Sbjct: 3 DIKVTPGTSELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY 62 Query: 66 NDCVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKCVETFDLLNYYLTQSLQKEI 125 ND VLSYNASEFINEGKNELDPEADSFDKVILPIASMFVK VETFDLLNYYLTQSLQKEI Sbjct: 63 NDSVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKSVETFDLLNYYLTQSLQKEI 122 Query: 126 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKCADEDG 185 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIK ADEDG Sbjct: 123 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKSADEDG 182 Query: 186 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKLK 245 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKLK Sbjct: 183 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKLK 242 Query: 246 N 246 N Sbjct: 243 N 243 >1XLY-A nrPDB Length = 234 Score = 466 bits (1200), Expect = e-132, Method: Compositional matrix adjust. Identities = 230/234 (98%), Positives = 230/234 (98%) Query: 6 DIKVTPGTCELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY 65 DIKVTPGT ELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY Sbjct: 1 DIKVTPGTSELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY 60 Query: 66 NDCVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKCVETFDLLNYYLTQSLQKEI 125 ND VLSYNASEFINEGKNELDPEADSFDKVILPIASMFVK VETFDLLNYYLTQSLQKEI Sbjct: 61 NDSVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKSVETFDLLNYYLTQSLQKEI 120 Query: 126 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKCADEDG 185 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIK ADEDG Sbjct: 121 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKSADEDG 180 Query: 186 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHD 239 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHD Sbjct: 181 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHD 234 >2GAK-A nrPDB Length = 391 Score = 28.9 bits (63), Expect = 2.0, Method: Compositional matrix adjust. Identities = 11/27 (40%), Positives = 17/27 (62%) Query: 157 WMIESLRIGSNLLDLEVVQFAIKCADE 183 WM+ + +N D++V FAI+C DE Sbjct: 353 WMLRQHHLFANKFDMDVDPFAIQCLDE 379 >6DKT-A nrPDB Length = 296 Score = 28.5 bits (62), Expect = 2.5, Method: Compositional matrix adjust. Identities = 14/48 (29%), Positives = 24/48 (50%) Query: 197 QEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKL 244 Q++ V E++F + HSI G L+ + +D + W+D G L Sbjct: 78 QKVNKVIEEKKFPLVLGGDHSIAIGTLAGTAKHYDNLGVIWYDAHGDL 125 >2AU3-A nrPDB Length = 407 Score = 28.1 bits (61), Expect = 3.2, Method: Compositional matrix adjust. Identities = 30/100 (30%), Positives = 45/100 (45%), Gaps = 10/100 (10%) Query: 64 FYNDCVL-SYNASEFINEGKNELDPE-ADSFDKVILPIASMFVKCVETFDLLNYYLTQSL 121 FY + +L + ASE++ +DP+ A FD P + VK ++ DLL YL Sbjct: 115 FYRESLLKNREASEYVKS--RGIDPKVARKFDLGYAPSSEALVKVLKENDLLEAYLE--- 169 Query: 122 QKEILSKT--LNEDLTLTAESILAIDDTYNHFVKFSQWMI 159 K +LS T + DL L ++ I D + F I Sbjct: 170 TKNLLSPTKGVYRDLFLR-RVVIPIKDPRGRVIGFGGRRI 208 >4LDP-A nrPDB Length = 455 Score = 28.1 bits (61), Expect = 3.7, Method: Compositional matrix adjust. Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 4/69 (5%) Query: 136 LTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKCADEDGTNIGE-TDNI 194 LT + + + Y Q M+E L G+ LD+EV+ +D++ +GE N+ Sbjct: 270 LTKRRLSRVTEQYGEQSDQEQAMVERLLRGAARLDVEVIA---TLSDDEVREMGELPSNV 326 Query: 195 FLQEILPVN 203 + E +P+N Sbjct: 327 RVHEYVPLN 335 >2Z4T-A nrPDB Length = 514 Score = 26.6 bits (57), Expect = 9.4, Method: Compositional matrix adjust. Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 13/101 (12%) Query: 128 KTLNEDLTLTAESILAIDDTYNHFVKFSQWM-----IESLRIGSNLLDLEVVQFAIKCAD 182 K LN +T +I DD +V W ++L+IG + L + I + Sbjct: 214 KELNSFNNVTVTNIDLYDDGSAEYVNLYNWRDTLNKTDNLKIGKDYL-----EDVINGIN 268 Query: 183 EDGTNIGETDNIFLQEILPVNS---EEEFQTLSAAWHSILD 220 ED +N G + Q++ P N +++ TL + H + D Sbjct: 269 EDTSNTGTSSVYNWQKLYPANYHFLRKDYLTLEPSLHELRD 309 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5M0J-D nrPDB 461 e-130 1XLY-A nrPDB 422 e-119 Sequences not found previously or not previously below threshold: 3OGM-B nrPDB 30 1.4 4G9I-A nrPDB 29 1.7 2GAK-A nrPDB 28 3.9 4LDP-A nrPDB 28 4.5 6DKT-A nrPDB 28 4.6 6D1O-A nrPDB 28 5.6 4I6V-A nrPDB 27 7.4 6BXJ-A nrPDB 27 8.3 6CKB-A nrPDB 27 9.7 CONVERGED! >5M0J-D nrPDB Length = 328 Score = 461 bits (1187), Expect = e-130, Method: Composition-based stats. Identities = 237/241 (98%), Positives = 237/241 (98%) Query: 6 DIKVTPGTCELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY 65 DIKVTPGT ELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY Sbjct: 3 DIKVTPGTSELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY 62 Query: 66 NDCVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKCVETFDLLNYYLTQSLQKEI 125 ND VLSYNASEFINEGKNELDPEADSFDKVILPIASMFVK VETFDLLNYYLTQSLQKEI Sbjct: 63 NDSVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKSVETFDLLNYYLTQSLQKEI 122 Query: 126 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKCADEDG 185 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIK ADEDG Sbjct: 123 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKSADEDG 182 Query: 186 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKLK 245 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKLK Sbjct: 183 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKLK 242 Query: 246 N 246 N Sbjct: 243 N 243 >1XLY-A nrPDB Length = 234 Score = 422 bits (1086), Expect = e-119, Method: Composition-based stats. Identities = 230/234 (98%), Positives = 230/234 (98%) Query: 6 DIKVTPGTCELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY 65 DIKVTPGT ELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY Sbjct: 1 DIKVTPGTSELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFY 60 Query: 66 NDCVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKCVETFDLLNYYLTQSLQKEI 125 ND VLSYNASEFINEGKNELDPEADSFDKVILPIASMFVK VETFDLLNYYLTQSLQKEI Sbjct: 61 NDSVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKSVETFDLLNYYLTQSLQKEI 120 Query: 126 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKCADEDG 185 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIK ADEDG Sbjct: 121 LSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKSADEDG 180 Query: 186 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHD 239 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHD Sbjct: 181 TNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHD 234 >3OGM-B nrPDB Length = 592 Score = 29.5 bits (65), Expect = 1.4, Method: Composition-based stats. Identities = 24/108 (22%), Positives = 52/108 (48%), Gaps = 10/108 (9%) Query: 35 LNKFISHLRRVATLRFERTTLI----KFVKKLRFYNDC--VLSYNASEFINEGKNELDPE 88 L ++H R++ TL E ++ K++ +L +N VL++ +EF +L+ Sbjct: 156 LLSIVTHCRKIKTLLMEESSFSEKDGKWLHELAQHNTSLEVLNFYMTEFAKISPKDLETI 215 Query: 89 ADSFDKVILPIASMFVKCVETFDLLNYYLTQSLQKEILSKTLNEDLTL 136 A + ++ S+ V E +L+ ++ + +E +LNED+ + Sbjct: 216 ARNCRSLV----SVKVGDFEILELVGFFKAAANLEEFCGGSLNEDIGM 259 >4G9I-A nrPDB Length = 772 Score = 29.2 bits (64), Expect = 1.7, Method: Composition-based stats. Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 6/59 (10%) Query: 36 NKFISHLRRVATLRFERTTLIKFVKKLRFYN------DCVLSYNASEFINEGKNELDPE 88 +++I + +V L F R + F K LR N D +YN ++ E NELD E Sbjct: 422 SQYIGNTGKVEVLEFMREAIAHFRKILRVKNLDLIIADLHPAYNTTKLAMEMANELDVE 480 >2GAK-A nrPDB Length = 391 Score = 28.0 bits (61), Expect = 3.9, Method: Composition-based stats. Identities = 11/27 (40%), Positives = 17/27 (62%) Query: 157 WMIESLRIGSNLLDLEVVQFAIKCADE 183 WM+ + +N D++V FAI+C DE Sbjct: 353 WMLRQHHLFANKFDMDVDPFAIQCLDE 379 >4LDP-A nrPDB Length = 455 Score = 27.6 bits (60), Expect = 4.5, Method: Composition-based stats. Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 4/69 (5%) Query: 136 LTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKCADEDGTNIGE-TDNI 194 LT + + + Y Q M+E L G+ LD+EV+ +D++ +GE N+ Sbjct: 270 LTKRRLSRVTEQYGEQSDQEQAMVERLLRGAARLDVEVIA---TLSDDEVREMGELPSNV 326 Query: 195 FLQEILPVN 203 + E +P+N Sbjct: 327 RVHEYVPLN 335 >6DKT-A nrPDB Length = 296 Score = 27.6 bits (60), Expect = 4.6, Method: Composition-based stats. Identities = 14/48 (29%), Positives = 24/48 (50%) Query: 197 QEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKL 244 Q++ V E++F + HSI G L+ + +D + W+D G L Sbjct: 78 QKVNKVIEEKKFPLVLGGDHSIAIGTLAGTAKHYDNLGVIWYDAHGDL 125 >6D1O-A nrPDB Length = 301 Score = 27.6 bits (60), Expect = 5.6, Method: Composition-based stats. Identities = 23/74 (31%), Positives = 33/74 (44%), Gaps = 15/74 (20%) Query: 174 VQFAIKCADEDGTNIGE---TDNIFL-----------QEILPVNSEEEFQTLSAAWHSIL 219 VQ + A+E G IGE TD+ FL +EI P + F ++ AW + L Sbjct: 92 VQMIRREANESGRVIGENWHTDSTFLDAPPAAVVMYAKEIPPYGGDTLFTSMYTAWET-L 150 Query: 220 DGKLSALDEEFDVV 233 + A E +VV Sbjct: 151 SPTMQATIEGLNVV 164 >4I6V-A nrPDB Length = 434 Score = 27.2 bits (59), Expect = 7.4, Method: Composition-based stats. Identities = 19/70 (27%), Positives = 32/70 (45%), Gaps = 7/70 (10%) Query: 83 NELDPEADSFDKVILPIASMFVKCVETFDLLNYYLTQSLQKEILSKTLNEDLTLTAESIL 142 N+ D FD ++ C+ T DL+ ++L + ++ L+K D A+ I Sbjct: 147 NDFDDPLTGFD------TKKYIPCLRTDDLV-FHLQKPEVRQRLAKATGFDCATPAKLIH 199 Query: 143 AIDDTYNHFV 152 AI + HFV Sbjct: 200 AIGQLFTHFV 209 >6BXJ-A nrPDB Length = 625 Score = 26.8 bits (58), Expect = 8.3, Method: Composition-based stats. Identities = 20/48 (41%), Positives = 27/48 (56%), Gaps = 1/48 (2%) Query: 100 ASMFVKCVETFDLLNYYLTQSLQKEILSKTLNEDLTLTAESILAIDDT 147 AS FVK ++TF+ L T+ LQK+I SK E L E L+ + T Sbjct: 264 ASEFVKILDTFEKLKDLFTE-LQKKIRSKVELEVRDLPEELSLSFNAT 310 >6CKB-A nrPDB Length = 466 Score = 26.8 bits (58), Expect = 9.7, Method: Composition-based stats. Identities = 20/48 (41%), Positives = 27/48 (56%), Gaps = 1/48 (2%) Query: 100 ASMFVKCVETFDLLNYYLTQSLQKEILSKTLNEDLTLTAESILAIDDT 147 AS FVK ++TF+ L T+ LQK+I SK E L E L+ + T Sbjct: 264 ASEFVKILDTFEKLKDLFTE-LQKKIRSKVELEVRDLPEELSLSFNAT 310 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.144 0.422 Lambda K H 0.267 0.0441 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,646,207 Number of Sequences: 36641 Number of extensions: 541117 Number of successful extensions: 1157 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 23 Number of HSP's that attempted gapping in prelim test: 1142 Number of HSP's gapped (non-prelim): 33 length of query: 246 length of database: 10,336,785 effective HSP length: 94 effective length of query: 152 effective length of database: 6,892,531 effective search space: 1047664712 effective search space used: 1047664712 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.8 bits)