BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P35203 (478 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6FE8-D nrPDB 978 0.0 6MZM-L nrPDB 33 0.39 5T5H-O nrPDB 30 2.9 1TAF-A nrPDB 29 3.5 4V8M-BO nrPDB 29 5.2 6HMA-F nrPDB 28 6.6 4WFB-D nrPDB 28 6.7 6AZ3-H nrPDB 28 7.6 6F3T-F nrPDB 28 8.3 >6FE8-D nrPDB Length = 519 Score = 978 bits (2529), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/477 (100%), Positives = 477/477 (100%) Query: 2 PSFNPVRFLELPIDIRKEVYFHLDGNFCGAHPYPIDILYKSNDVELPGKPSYKRSKRSKK 61 PSFNPVRFLELPIDIRKEVYFHLDGNFCGAHPYPIDILYKSNDVELPGKPSYKRSKRSKK Sbjct: 3 PSFNPVRFLELPIDIRKEVYFHLDGNFCGAHPYPIDILYKSNDVELPGKPSYKRSKRSKK 62 Query: 62 LLRYMYPVFATYLNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKVNHLYDGTLIDALEWT 121 LLRYMYPVFATYLNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKVNHLYDGTLIDALEWT Sbjct: 63 LLRYMYPVFATYLNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKVNHLYDGTLIDALEWT 122 Query: 122 YLDNELRLAYFNKASMLEVWYTFKEYKKWVIDSVAFDELDLLNVSNIQFNIDNLTPQLVD 181 YLDNELRLAYFNKASMLEVWYTFKEYKKWVIDSVAFDELDLLNVSNIQFNIDNLTPQLVD Sbjct: 123 YLDNELRLAYFNKASMLEVWYTFKEYKKWVIDSVAFDELDLLNVSNIQFNIDNLTPQLVD 182 Query: 182 KCLSILEQKDLFATIGEVQFGQDEEVGEEKDVDVSGANSDENSSPSSTIKNKKRSASKRS 241 KCLSILEQKDLFATIGEVQFGQDEEVGEEKDVDVSGANSDENSSPSSTIKNKKRSASKRS Sbjct: 183 KCLSILEQKDLFATIGEVQFGQDEEVGEEKDVDVSGANSDENSSPSSTIKNKKRSASKRS 242 Query: 242 HSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTI 301 HSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTI Sbjct: 243 HSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTI 302 Query: 302 SYIVKRRINEIRLSRMNQISRTGLADFTRWDNLQKLVLSRVAYIDLNSIVFPKNFKSLTM 361 SYIVKRRINEIRLSRMNQISRTGLADFTRWDNLQKLVLSRVAYIDLNSIVFPKNFKSLTM Sbjct: 303 SYIVKRRINEIRLSRMNQISRTGLADFTRWDNLQKLVLSRVAYIDLNSIVFPKNFKSLTM 362 Query: 362 KRVSKIKWWNIEENILKELKVDKRTFKSLYIKEDDSKFTKFFNLRHTRIKELDKSEINQI 421 KRVSKIKWWNIEENILKELKVDKRTFKSLYIKEDDSKFTKFFNLRHTRIKELDKSEINQI Sbjct: 363 KRVSKIKWWNIEENILKELKVDKRTFKSLYIKEDDSKFTKFFNLRHTRIKELDKSEINQI 422 Query: 422 TYLRCQAIVWLSFRTLNHIKLQNVSEVFNNIIVPRALFDSKRVEIYRCEKISQVLVI 478 TYLRCQAIVWLSFRTLNHIKLQNVSEVFNNIIVPRALFDSKRVEIYRCEKISQVLVI Sbjct: 423 TYLRCQAIVWLSFRTLNHIKLQNVSEVFNNIIVPRALFDSKRVEIYRCEKISQVLVI 479 >6MZM-L nrPDB Length = 264 Score = 32.7 bits (73), Expect = 0.39, Method: Compositional matrix adjust. Identities = 24/82 (29%), Positives = 43/82 (52%), Gaps = 15/82 (18%) Query: 74 LNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKV--NHLYDGTLIDALEWTYLDNELRLAY 131 + I EY P++I + LE+AF RY +LD K+ +H T +DA +++RLA Sbjct: 26 MGITEYEPRVINQMLEFAF--RYVTTILDDAKIYSSHAKKAT-VDA-------DDVRLAI 75 Query: 132 FNKASMLEVWYTFKEYKKWVID 153 +A + +T + +++D Sbjct: 76 QCRA---DQSFTSPPPRDFLLD 94 >5T5H-O nrPDB Length = 203 Score = 29.6 bits (65), Expect = 2.9, Method: Compositional matrix adjust. Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%) Query: 244 DNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTISY 303 N +G H++ S +RT+RSM L + T RG+K L+ + G N +T Sbjct: 63 SNPKLGPFHHRSPSDIFLRTVRSM-----LPRYTKRGQKALRQLVAYEGIPTNVVRTGGR 117 Query: 304 IV 305 +V Sbjct: 118 VV 119 >1TAF-A nrPDB Length = 68 Score = 29.3 bits (64), Expect = 3.5, Method: Compositional matrix adjust. Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 4/43 (9%) Query: 74 LNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKV--NHLYDGTL 114 LN+ EY P+++ + LE+ F RY +LD KV NH T+ Sbjct: 14 LNVQEYEPRVVNQLLEFTF--RYVTSILDDAKVYANHARKKTI 54 >4V8M-BO nrPDB Length = 222 Score = 28.9 bits (63), Expect = 5.2, Method: Compositional matrix adjust. Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%) Query: 244 DNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTISY 303 N +G H++ S IRT+RSM L + T RG++ L+ + G N +T Sbjct: 82 SNPKLGPFHHRSPSDVFIRTVRSM-----LPRYTKRGQRALRQLVAYEGVPVNVVRTGGR 136 Query: 304 IV 305 +V Sbjct: 137 VV 138 >6HMA-F nrPDB Length = 158 Score = 28.5 bits (62), Expect = 6.6, Method: Composition-based stats. Identities = 13/27 (48%), Positives = 17/27 (62%) Query: 261 IRTIRSMESMKSLRKITVRGEKLYELL 287 I T R E M K+T+RGE++YE L Sbjct: 56 IATFRLREGMPIGAKVTLRGERMYEFL 82 >4WFB-D nrPDB Length = 179 Score = 28.5 bits (62), Expect = 6.7, Method: Compositional matrix adjust. Identities = 13/27 (48%), Positives = 17/27 (62%) Query: 261 IRTIRSMESMKSLRKITVRGEKLYELL 287 I T R E M K+T+RGE++YE L Sbjct: 74 IATFRLREGMPIGAKVTLRGERMYEFL 100 >6AZ3-H nrPDB Length = 221 Score = 28.5 bits (62), Expect = 7.6, Method: Compositional matrix adjust. Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 5/61 (8%) Query: 245 NGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTISYI 304 N G H++ S +RT+RSM L + T RG K L+ + G N +T + Sbjct: 82 NPTKGPFHHRAPSDVFVRTVRSM-----LPRYTKRGMKALNSLVAYEGIPPNVVRTGGRV 136 Query: 305 V 305 V Sbjct: 137 V 137 >6F3T-F nrPDB Length = 116 Score = 28.1 bits (61), Expect = 8.3, Method: Composition-based stats. Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 2/33 (6%) Query: 74 LNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKV 106 + I EY P++I + LE+AF RY +LD K+ Sbjct: 22 MGITEYEPRVINQMLEFAF--RYVTTILDDAKI 52 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6FE8-D nrPDB 999 0.0 Sequences not found previously or not previously below threshold: 5Y58-A nrPDB 32 0.66 5LJ5-W nrPDB 30 2.5 5T5H-O nrPDB 30 3.1 4V8M-BO nrPDB 30 3.1 5WLC-SS nrPDB 29 4.9 1TAF-A nrPDB 29 4.9 6MZM-L nrPDB 28 6.5 6F3T-F nrPDB 28 6.5 4A35-A nrPDB 28 7.0 CONVERGED! >6FE8-D nrPDB Length = 519 Score = 999 bits (2582), Expect = 0.0, Method: Composition-based stats. Identities = 477/477 (100%), Positives = 477/477 (100%) Query: 2 PSFNPVRFLELPIDIRKEVYFHLDGNFCGAHPYPIDILYKSNDVELPGKPSYKRSKRSKK 61 PSFNPVRFLELPIDIRKEVYFHLDGNFCGAHPYPIDILYKSNDVELPGKPSYKRSKRSKK Sbjct: 3 PSFNPVRFLELPIDIRKEVYFHLDGNFCGAHPYPIDILYKSNDVELPGKPSYKRSKRSKK 62 Query: 62 LLRYMYPVFATYLNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKVNHLYDGTLIDALEWT 121 LLRYMYPVFATYLNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKVNHLYDGTLIDALEWT Sbjct: 63 LLRYMYPVFATYLNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKVNHLYDGTLIDALEWT 122 Query: 122 YLDNELRLAYFNKASMLEVWYTFKEYKKWVIDSVAFDELDLLNVSNIQFNIDNLTPQLVD 181 YLDNELRLAYFNKASMLEVWYTFKEYKKWVIDSVAFDELDLLNVSNIQFNIDNLTPQLVD Sbjct: 123 YLDNELRLAYFNKASMLEVWYTFKEYKKWVIDSVAFDELDLLNVSNIQFNIDNLTPQLVD 182 Query: 182 KCLSILEQKDLFATIGEVQFGQDEEVGEEKDVDVSGANSDENSSPSSTIKNKKRSASKRS 241 KCLSILEQKDLFATIGEVQFGQDEEVGEEKDVDVSGANSDENSSPSSTIKNKKRSASKRS Sbjct: 183 KCLSILEQKDLFATIGEVQFGQDEEVGEEKDVDVSGANSDENSSPSSTIKNKKRSASKRS 242 Query: 242 HSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTI 301 HSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTI Sbjct: 243 HSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYELLINFHGFRDNPGKTI 302 Query: 302 SYIVKRRINEIRLSRMNQISRTGLADFTRWDNLQKLVLSRVAYIDLNSIVFPKNFKSLTM 361 SYIVKRRINEIRLSRMNQISRTGLADFTRWDNLQKLVLSRVAYIDLNSIVFPKNFKSLTM Sbjct: 303 SYIVKRRINEIRLSRMNQISRTGLADFTRWDNLQKLVLSRVAYIDLNSIVFPKNFKSLTM 362 Query: 362 KRVSKIKWWNIEENILKELKVDKRTFKSLYIKEDDSKFTKFFNLRHTRIKELDKSEINQI 421 KRVSKIKWWNIEENILKELKVDKRTFKSLYIKEDDSKFTKFFNLRHTRIKELDKSEINQI Sbjct: 363 KRVSKIKWWNIEENILKELKVDKRTFKSLYIKEDDSKFTKFFNLRHTRIKELDKSEINQI 422 Query: 422 TYLRCQAIVWLSFRTLNHIKLQNVSEVFNNIIVPRALFDSKRVEIYRCEKISQVLVI 478 TYLRCQAIVWLSFRTLNHIKLQNVSEVFNNIIVPRALFDSKRVEIYRCEKISQVLVI Sbjct: 423 TYLRCQAIVWLSFRTLNHIKLQNVSEVFNNIIVPRALFDSKRVEIYRCEKISQVLVI 479 >5Y58-A nrPDB Length = 575 Score = 31.9 bits (71), Expect = 0.66, Method: Composition-based stats. Identities = 30/109 (27%), Positives = 55/109 (50%), Gaps = 11/109 (10%) Query: 331 WDNLQKLVLSRVAYIDLNSIVFPKNFKSLTMKRVSKIKWWNIEENILK-ELKVDK----R 385 +DN++K+ S + Y +L P +FK+ +++ K+ + + +L+ E D+ Sbjct: 447 YDNMKKVTQSIMGYFNLRDGYNPSDFKNPLLQKHYKV----LHDYLLQIETTFDENETPN 502 Query: 386 TFKSLYIKEDDSKFTKFFNLRHTRIKELDKSEINQITYLRCQAIVWLSF 434 T K ++EDDS K + +R+ +I E +KSE I L +W F Sbjct: 503 TKKDRMMREDDS-LRKLYYIRN-KILESEKSEDPIIQRLNKYVKIWNMF 549 >5LJ5-W nrPDB Length = 238 Score = 30.0 bits (66), Expect = 2.5, Method: Composition-based stats. Identities = 17/66 (25%), Positives = 36/66 (54%), Gaps = 5/66 (7%) Query: 325 LADFTRWDNLQKLVLSRVAYIDLNSIVFPKNFKSLTMKRVSKIKWWNIEENILKELKVDK 384 + D +R D++ L+L R ++++ + P N ++LT+ S ++ + L+ L+ Sbjct: 67 IPDLSRRDDIHTLLLGRNNIVEVDGRLLPMNVQNLTLSNNSIRRFED-----LQRLRRAP 121 Query: 385 RTFKSL 390 RT K+L Sbjct: 122 RTLKNL 127 >5T5H-O nrPDB Length = 203 Score = 29.6 bits (65), Expect = 3.1, Method: Composition-based stats. Identities = 22/93 (23%), Positives = 43/93 (46%), Gaps = 7/93 (7%) Query: 215 VSGANSDENSSPSSTIKNKKRSAS--KRSHSDNGNVGATHNQLTSISVIRTIRSMESMKS 272 ++ ++ + + I+NK + ++ N +G H++ S +RT+RSM Sbjct: 32 ITAVRCEQLTIAGTEIRNKIKYLQFLRKRKLSNPKLGPFHHRSPSDIFLRTVRSM----- 86 Query: 273 LRKITVRGEKLYELLINFHGFRDNPGKTISYIV 305 L + T RG+K L+ + G N +T +V Sbjct: 87 LPRYTKRGQKALRQLVAYEGIPTNVVRTGGRVV 119 >4V8M-BO nrPDB Length = 222 Score = 29.6 bits (65), Expect = 3.1, Method: Composition-based stats. Identities = 23/80 (28%), Positives = 38/80 (47%), Gaps = 7/80 (8%) Query: 228 STIKNKKRSAS--KRSHSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKITVRGEKLYE 285 S I+NK + ++ N +G H++ S IRT+RSM L + T RG++ Sbjct: 64 SEIRNKIKYLQFLRKRKLSNPKLGPFHHRSPSDVFIRTVRSM-----LPRYTKRGQRALR 118 Query: 286 LLINFHGFRDNPGKTISYIV 305 L+ + G N +T +V Sbjct: 119 QLVAYEGVPVNVVRTGGRVV 138 >5WLC-SS nrPDB Length = 898 Score = 28.8 bits (63), Expect = 4.9, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 24/47 (51%), Gaps = 6/47 (12%) Query: 200 QFGQDEEVGEEKDVDVSGANSDENSSPSSTIKNKKRSASKRSHSDNG 246 + DE +G + D D+ NS S TI++KK +A+ + D G Sbjct: 76 ELNSDEALGSDDDYDIL------NSKFSQTIRDKKENANYQEEEDEG 116 >1TAF-A nrPDB Length = 68 Score = 28.8 bits (63), Expect = 4.9, Method: Composition-based stats. Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 4/43 (9%) Query: 74 LNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKV--NHLYDGTL 114 LN+ EY P+++ + LE+ F RY +LD KV NH T+ Sbjct: 14 LNVQEYEPRVVNQLLEFTF--RYVTSILDDAKVYANHARKKTI 54 >6MZM-L nrPDB Length = 264 Score = 28.5 bits (62), Expect = 6.5, Method: Composition-based stats. Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 2/33 (6%) Query: 74 LNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKV 106 + I EY P++I + LE+AF RY +LD K+ Sbjct: 26 MGITEYEPRVINQMLEFAF--RYVTTILDDAKI 56 >6F3T-F nrPDB Length = 116 Score = 28.5 bits (62), Expect = 6.5, Method: Composition-based stats. Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 2/33 (6%) Query: 74 LNIFEYSPQLIEKWLEYAFWLRYDCLVLDCFKV 106 + I EY P++I + LE+AF RY +LD K+ Sbjct: 22 MGITEYEPRVINQMLEFAF--RYVTTILDDAKI 52 >4A35-A nrPDB Length = 441 Score = 28.5 bits (62), Expect = 7.0, Method: Composition-based stats. Identities = 16/66 (24%), Positives = 30/66 (45%), Gaps = 3/66 (4%) Query: 34 YPIDILYKSNDVELPGKPSYKRSKRSKKLLRYMYPVFATYLNIFEYSPQLIEKWLEYAF- 92 Y D+L + + +E+ K + +R K++L YP + T YS +++ A Sbjct: 155 YITDVLTEEDALEILQKGQIGKKEREKQMLAQGYPAYTTSCAWLGYSDDTLKQLCAQALK 214 Query: 93 --WLRY 96 W R+ Sbjct: 215 DGWTRF 220 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.137 0.406 Lambda K H 0.267 0.0448 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 21,761,198 Number of Sequences: 36641 Number of extensions: 1012334 Number of successful extensions: 2185 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 27 Number of HSP's that attempted gapping in prelim test: 2175 Number of HSP's gapped (non-prelim): 39 length of query: 478 length of database: 10,336,785 effective HSP length: 101 effective length of query: 377 effective length of database: 6,636,044 effective search space: 2501788588 effective search space used: 2501788588 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 61 (28.0 bits)