BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P34162 (210 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5OQM-f nrPDB 432 e-122 5OFO-C nrPDB 28 2.0 4CIU-A nrPDB 28 2.0 1JBK-A nrPDB 28 3.1 1M41-A nrPDB 28 3.6 1OKI-A nrPDB 27 6.4 >5OQM-f nrPDB Length = 210 Score = 432 bits (1111), Expect = e-122, Method: Compositional matrix adjust. Identities = 210/210 (100%), Positives = 210/210 (100%) Query: 1 MGKSAVIFVERATPATLTELKDALSNSILSVRDPWSIDFRTYRCSIKNLPADVSKLMYSI 60 MGKSAVIFVERATPATLTELKDALSNSILSVRDPWSIDFRTYRCSIKNLPADVSKLMYSI Sbjct: 1 MGKSAVIFVERATPATLTELKDALSNSILSVRDPWSIDFRTYRCSIKNLPADVSKLMYSI 60 Query: 61 TFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPESIDTILSSKLSNIWMQRQ 120 TFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPESIDTILSSKLSNIWMQRQ Sbjct: 61 TFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPESIDTILSSKLSNIWMQRQ 120 Query: 121 LIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEAGEFETKIAGIEGHLAEIRAK 180 LIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEAGEFETKIAGIEGHLAEIRAK Sbjct: 121 LIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEAGEFETKIAGIEGHLAEIRAK 180 Query: 181 EYKTSSDSLGPDTSNEICDLAYQYVRALEL 210 EYKTSSDSLGPDTSNEICDLAYQYVRALEL Sbjct: 181 EYKTSSDSLGPDTSNEICDLAYQYVRALEL 210 >5OFO-C nrPDB Length = 871 Score = 28.5 bits (62), Expect = 2.0, Method: Compositional matrix adjust. Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 8/58 (13%) Query: 127 GETLILDGLTVRLVNLFSSTGFKG---LLIELQADEA-----GEFETKIAGIEGHLAE 176 G+T I++GL R++N G KG L +++ A A GEFE ++ G+ LA+ Sbjct: 215 GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAK 272 >4CIU-A nrPDB Length = 727 Score = 28.5 bits (62), Expect = 2.0, Method: Compositional matrix adjust. Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 8/58 (13%) Query: 127 GETLILDGLTVRLVNLFSSTGFKG---LLIELQADEA-----GEFETKIAGIEGHLAE 176 G+T I++GL R++N G KG L +++ A A GEFE ++ G+ LA+ Sbjct: 69 GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAK 126 >1JBK-A nrPDB Length = 195 Score = 28.1 bits (61), Expect = 3.1, Method: Compositional matrix adjust. Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 8/58 (13%) Query: 127 GETLILDGLTVRLVNLFSSTGFKG---LLIELQADEA-----GEFETKIAGIEGHLAE 176 G+T I++GL R++N G KG L +++ A A GEFE ++ G+ LA+ Sbjct: 55 GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAK 112 >1M41-A nrPDB Length = 380 Score = 27.7 bits (60), Expect = 3.6, Method: Compositional matrix adjust. Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Query: 114 NIWMQRQLIKGDAGETLILDGLTVRL-VNLFSSTGFKGLLI 153 N+W L++G AG L+ DG TV +N +++ G ++ Sbjct: 288 NLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVL 328 >1OKI-A nrPDB Length = 210 Score = 26.9 bits (58), Expect = 6.4, Method: Compositional matrix adjust. Identities = 21/56 (37%), Positives = 27/56 (48%), Gaps = 9/56 (16%) Query: 105 DTILSSKLSNIWMQRQLIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEA 160 +T SS S+ M + IK DA E I +LF FKG IE+Q D+A Sbjct: 83 NTWSSSYRSDRLMSFRPIKMDAQEHKI---------SLFEGANFKGNTIEIQGDDA 129 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5OQM-f nrPDB 436 e-123 Sequences not found previously or not previously below threshold: 5OFO-C nrPDB 30 0.84 4CIU-A nrPDB 30 0.84 1JBK-A nrPDB 30 0.84 1M41-A nrPDB 27 4.4 1VQ0-A nrPDB 27 7.1 4LUQ-A nrPDB 27 7.2 6BN2-A nrPDB 27 8.5 CONVERGED! >5OQM-f nrPDB Length = 210 Score = 436 bits (1121), Expect = e-123, Method: Composition-based stats. Identities = 210/210 (100%), Positives = 210/210 (100%) Query: 1 MGKSAVIFVERATPATLTELKDALSNSILSVRDPWSIDFRTYRCSIKNLPADVSKLMYSI 60 MGKSAVIFVERATPATLTELKDALSNSILSVRDPWSIDFRTYRCSIKNLPADVSKLMYSI Sbjct: 1 MGKSAVIFVERATPATLTELKDALSNSILSVRDPWSIDFRTYRCSIKNLPADVSKLMYSI 60 Query: 61 TFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPESIDTILSSKLSNIWMQRQ 120 TFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPESIDTILSSKLSNIWMQRQ Sbjct: 61 TFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPESIDTILSSKLSNIWMQRQ 120 Query: 121 LIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEAGEFETKIAGIEGHLAEIRAK 180 LIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEAGEFETKIAGIEGHLAEIRAK Sbjct: 121 LIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEAGEFETKIAGIEGHLAEIRAK 180 Query: 181 EYKTSSDSLGPDTSNEICDLAYQYVRALEL 210 EYKTSSDSLGPDTSNEICDLAYQYVRALEL Sbjct: 181 EYKTSSDSLGPDTSNEICDLAYQYVRALEL 210 >5OFO-C nrPDB Length = 871 Score = 29.7 bits (65), Expect = 0.84, Method: Composition-based stats. Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 8/58 (13%) Query: 127 GETLILDGLTVRLVNLFSSTGFKG---LLIELQADEA-----GEFETKIAGIEGHLAE 176 G+T I++GL R++N G KG L +++ A A GEFE ++ G+ LA+ Sbjct: 215 GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAK 272 >4CIU-A nrPDB Length = 727 Score = 29.7 bits (65), Expect = 0.84, Method: Composition-based stats. Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 8/58 (13%) Query: 127 GETLILDGLTVRLVNLFSSTGFKG---LLIELQADEA-----GEFETKIAGIEGHLAE 176 G+T I++GL R++N G KG L +++ A A GEFE ++ G+ LA+ Sbjct: 69 GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAK 126 >1JBK-A nrPDB Length = 195 Score = 29.7 bits (65), Expect = 0.84, Method: Composition-based stats. Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 8/58 (13%) Query: 127 GETLILDGLTVRLVNLFSSTGFKG---LLIELQADEA-----GEFETKIAGIEGHLAE 176 G+T I++GL R++N G KG L +++ A A GEFE ++ G+ LA+ Sbjct: 55 GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAK 112 >1M41-A nrPDB Length = 380 Score = 27.4 bits (59), Expect = 4.4, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Query: 114 NIWMQRQLIKGDAGETLILDGLTVRL-VNLFSSTGFKGLLI 153 N+W L++G AG L+ DG TV +N +++ G ++ Sbjct: 288 NLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVL 328 >1VQ0-A nrPDB Length = 302 Score = 26.6 bits (57), Expect = 7.1, Method: Composition-based stats. Identities = 20/53 (37%), Positives = 27/53 (50%), Gaps = 1/53 (1%) Query: 153 IELQADEAGEFETKIAGIEGHLAEIRAKEYKTSSDSLGPDTSNEIC-DLAYQY 204 +EL +E G+F+ A +G L +R KT S P S EI DLAY + Sbjct: 104 VELPLNEKGKFDVAGAIGQGVLRVVRDLGLKTPFVSQVPLVSGEIAEDLAYYF 156 >4LUQ-A nrPDB Length = 428 Score = 26.6 bits (57), Expect = 7.2, Method: Composition-based stats. Identities = 29/117 (24%), Positives = 48/117 (41%), Gaps = 3/117 (2%) Query: 58 YSITFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPESIDTILSSKLSNIWM 117 + +T R I++++ T AA D +L+ G PE ++++S M Sbjct: 39 WQVTRQEARRVIAAIRNDNVPDATIAALDKSGSLIKLFQRVGPPELARSLIASIAGRTTM 98 Query: 118 QRQLIKGDAGETLILDGLTVRLVN--LFSSTGFKGLLIELQADEAGEFETKIAGIEG 172 QR + +LI + L + N F + F + +L AD AG AG G Sbjct: 99 QRYQARNALIRSLINNPLGTQTDNWIYFPTITFFDICADL-ADAAGRLGFAAAGATG 154 >6BN2-A nrPDB Length = 401 Score = 26.6 bits (57), Expect = 8.5, Method: Composition-based stats. Identities = 26/109 (23%), Positives = 51/109 (46%), Gaps = 20/109 (18%) Query: 89 PALVFNGSSTGVPES----------IDTIL--SSKLSNIWMQRQLIKGD----AGETLIL 132 P F GS +GVP + +D I +++ +++M L G+ A + + Sbjct: 21 PMGSFMGSLSGVPATQLGAVAIKGALDKINLNPAEIQDVYMGNVLQAGEGQAPAKQAALG 80 Query: 133 DGLT----VRLVNLFSSTGFKGLLIELQADEAGEFETKIAGIEGHLAEI 177 GL VN ++G K +++ QA +AG+ E +AG +++++ Sbjct: 81 AGLPNTTPTTAVNKVCASGMKAVMMAAQAVKAGDVEAIVAGGMENMSQV 129 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.132 0.371 Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,728,842 Number of Sequences: 36641 Number of extensions: 274023 Number of successful extensions: 876 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 868 Number of HSP's gapped (non-prelim): 23 length of query: 210 length of database: 10,336,785 effective HSP length: 93 effective length of query: 117 effective length of database: 6,929,172 effective search space: 810713124 effective search space used: 810713124 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.6 bits)