BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P33754 (206 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6N3Q-E nrPDB 420 e-118 5L0W-A nrPDB 70 6e-13 2XPX-A nrPDB 30 1.0 3QNQ-A nrPDB 28 2.5 2A3L-A nrPDB 27 5.2 6GCS-5 nrPDB 27 7.7 3CF4-A nrPDB 26 9.3 >6N3Q-E nrPDB Length = 206 Score = 420 bits (1080), Expect = e-118, Method: Compositional matrix adjust. Identities = 206/206 (100%), Positives = 206/206 (100%) Query: 1 MSEFNETKFSNNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKIS 60 MSEFNETKFSNNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKIS Sbjct: 1 MSEFNETKFSNNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKIS 60 Query: 61 EQPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQINLL 120 EQPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQINLL Sbjct: 61 EQPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQINLL 120 Query: 121 YKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRY 180 YKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRY Sbjct: 121 YKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRY 180 Query: 181 QSILKRKEVCIKEWELKINNDGRLVN 206 QSILKRKEVCIKEWELKINNDGRLVN Sbjct: 181 QSILKRKEVCIKEWELKINNDGRLVN 206 >5L0W-A nrPDB Length = 184 Score = 70.1 bits (170), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 34/98 (34%), Positives = 54/98 (55%) Query: 86 KIHEKVLKAALLNRGAESVRRSLKLKELAPQINLLYKNGSIGEDYWKRFETEVKLIELEF 145 K+ + V++AALL R E + R ++++ + L + GS+G+D W+RF K +E E Sbjct: 53 KVPDSVIRAALLRRAVEDIHRIIQIRTAKAACSTLLQRGSVGDDLWQRFLRAEKEMEDEL 112 Query: 146 KDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRYQSI 183 +D + EA L PGW Q+ EI N+ L R + I Sbjct: 113 RDVVMEANALVPGWGQIIFQSANEIAANKVLRDRLEEI 150 >2XPX-A nrPDB Length = 173 Score = 29.6 bits (65), Expect = 1.0, Method: Compositional matrix adjust. Identities = 15/61 (24%), Positives = 26/61 (42%), Gaps = 7/61 (11%) Query: 122 KNGSIGEDYWKRFETEVKLIELEFKDTLQEA-ERLQP------GWVQLFVMVCKEICFNQ 174 +N + W RF T + ++L+F E R P W+ + C+ +C NQ Sbjct: 73 RNSETFTETWNRFITHTEHVDLDFNSVFLEIFHRGDPSLGRALAWMAWCMHACRTLCCNQ 132 Query: 175 A 175 + Sbjct: 133 S 133 >3QNQ-A nrPDB Length = 442 Score = 28.1 bits (61), Expect = 2.5, Method: Compositional matrix adjust. Identities = 12/31 (38%), Positives = 20/31 (64%) Query: 40 LVVSLVMFASSYRKKQAKKISEQPSIFDEND 70 LVV +++FA S + K ++S P IF+ N+ Sbjct: 304 LVVGMLLFARSQQLKSLGRLSIAPGIFNINE 334 >2A3L-A nrPDB Length = 701 Score = 27.3 bits (59), Expect = 5.2, Method: Compositional matrix adjust. Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 7/42 (16%) Query: 122 KNGSIGEDYWKR-------FETEVKLIELEFKDTLQEAERLQ 156 K+ IG+DY+KR +T V I +EF+DT+ + E Q Sbjct: 646 KSHWIGKDYYKRGPDGNDIHKTNVPHIRVEFRDTIWKEEMQQ 687 >6GCS-5 nrPDB Length = 655 Score = 26.6 bits (57), Expect = 7.7, Method: Composition-based stats. Identities = 27/102 (26%), Positives = 42/102 (41%), Gaps = 4/102 (3%) Query: 11 NNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKISEQPSIFDEND 70 NN ++F+TE I + + I V IL+V L F + K I+ SIF++ Sbjct: 495 NNFSYFDTEFSITQFYKLLPLISAILVSILIVVLNEFFAIVFNLNNKYINTVYSIFNQKL 554 Query: 71 AHDL----YFQIKEMSENEKIHEKVLKAALLNRGAESVRRSL 108 D + K + + I V K +L G + R L Sbjct: 555 VSDQILNHFIIFKGLVTSGNIAHHVDKGSLYRLGPVGINRLL 596 >3CF4-A nrPDB Length = 807 Score = 26.2 bits (56), Expect = 9.3, Method: Composition-based stats. Identities = 27/119 (22%), Positives = 51/119 (42%), Gaps = 9/119 (7%) Query: 48 ASSYRKKQAKKISEQPS--IFDENDAHDLYFQIKE---MSENEKIHEKV--LKAALLNRG 100 +S YR+ K E+ ++D + ++ ++ E E + + A + Sbjct: 658 SSKYRRALIAKTYEEDKWKVYDARNGQEMPIPPAPEFLLTTAETWQEAIPMMAKACIRPS 717 Query: 101 AESVRRSLKLKELAPQINLLYKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGW 159 S+ R++KL +++ Y G ED+WK TE L + L+E E+ + GW Sbjct: 718 DNSMGRAIKLTHWM-ELHKKYLGGKEPEDWWKFVRTEADLPLATREALLKELEK-EHGW 774 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6N3Q-E nrPDB 365 e-102 5L0W-A nrPDB 159 7e-40 Sequences not found previously or not previously below threshold: 3VUQ-A nrPDB 32 0.19 5HAF-A nrPDB 30 0.91 6ENZ-A nrPDB 29 1.3 5OQL-K nrPDB 29 1.5 5MUU-D nrPDB 29 1.5 5M6U-B nrPDB 29 1.7 5Z79-A nrPDB 28 2.0 6GCS-5 nrPDB 28 2.9 5UIA-A nrPDB 28 3.2 5OHN-A nrPDB 28 3.7 3QNQ-A nrPDB 27 5.4 3OPB-A nrPDB 27 5.6 3NZP-A nrPDB 27 8.2 CONVERGED! >6N3Q-E nrPDB Length = 206 Score = 365 bits (936), Expect = e-102, Method: Composition-based stats. Identities = 206/206 (100%), Positives = 206/206 (100%) Query: 1 MSEFNETKFSNNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKIS 60 MSEFNETKFSNNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKIS Sbjct: 1 MSEFNETKFSNNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKIS 60 Query: 61 EQPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQINLL 120 EQPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQINLL Sbjct: 61 EQPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQINLL 120 Query: 121 YKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRY 180 YKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRY Sbjct: 121 YKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRY 180 Query: 181 QSILKRKEVCIKEWELKINNDGRLVN 206 QSILKRKEVCIKEWELKINNDGRLVN Sbjct: 181 QSILKRKEVCIKEWELKINNDGRLVN 206 >5L0W-A nrPDB Length = 184 Score = 159 bits (402), Expect = 7e-40, Method: Composition-based stats. Identities = 46/153 (30%), Positives = 73/153 (47%), Gaps = 11/153 (7%) Query: 48 ASSYRKKQAKKISEQPSIFDENDAHDLYFQIKEMSENE------KIHEKVLKAALLNRGA 101 +S KK + + Q S N +Y + M E K+ + V++AALL R Sbjct: 13 GTSLYKKAGENLYFQGSHLQRN----IYLSLLHMEPEEGQEKAPKVPDSVIRAALLRRAV 68 Query: 102 ESVRRSLKLKELAPQINLLYKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQ 161 E + R ++++ + L + GS+G+D W+RF K +E E +D + EA L PGW Q Sbjct: 69 EDIHRIIQIRTAKAACSTLLQRGSVGDDLWQRFLRAEKEMEDELRDVVMEANALVPGWGQ 128 Query: 162 LFVMVCKEICFNQALSRRYQSILKRKEVCIKEW 194 + EI N+ L R + I + + KEW Sbjct: 129 IIFQSANEIAANKVLRDRLEEI-EAQTARDKEW 160 >3VUQ-A nrPDB Length = 187 Score = 32.0 bits (71), Expect = 0.19, Method: Composition-based stats. Identities = 22/99 (22%), Positives = 42/99 (42%), Gaps = 8/99 (8%) Query: 100 GAESVRRSLKLKELAPQINLLYKNGSIG---EDYWKRFETEVKLIELEFKDTLQEAERLQ 156 G + +R++ L + +L + G G E+ +R E I L F+D + L Sbjct: 9 GVKDKKRAI----LEATLAVLRERGLSGLKMEEVARRAEVGKGTIYLYFRDKRDLLKALV 64 Query: 157 PGWVQLFVMVCKEICFNQA-LSRRYQSILKRKEVCIKEW 194 F +E+ +A R + +L+R+ ++EW Sbjct: 65 EERTWAFYREVEEVVRRKAPFFVRLEEVLRRRLAWVQEW 103 >5HAF-A nrPDB Length = 338 Score = 29.6 bits (65), Expect = 0.91, Method: Composition-based stats. Identities = 16/62 (25%), Positives = 26/62 (41%) Query: 111 KELAPQINLLYKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEI 170 K+L LLY GS D K+ K++ +F T QE ++ W ++ E+ Sbjct: 104 KKLHAPSRLLYMAGSATTDLSKKIGIAHKIMGDQFAQTDQEQVGVENLWCGARMLSSDEL 163 Query: 171 CF 172 Sbjct: 164 AA 165 >6ENZ-A nrPDB Length = 530 Score = 29.3 bits (64), Expect = 1.3, Method: Composition-based stats. Identities = 25/100 (25%), Positives = 48/100 (48%), Gaps = 8/100 (8%) Query: 61 EQPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQI--- 117 E S++ + + +LYFQ M+ +KI + +LN V+ K E A ++ Sbjct: 11 ESTSLYKKAGSENLYFQ--GMTPGKKIP-IFVDGVVLNGPQTDVKAGEKFVEEACRLIME 67 Query: 118 NLLYKNGSIGEDY--WKRFETEVKLIELEFKDTLQEAERL 155 ++ K + E W+ E +L++LE +DT + ++L Sbjct: 68 EVVLKATDVNEKVCEWQPPEQLRQLLDLEMRDTGESQDKL 107 >5OQL-K nrPDB Length = 938 Score = 28.9 bits (63), Expect = 1.5, Method: Composition-based stats. Identities = 15/60 (25%), Positives = 27/60 (45%), Gaps = 7/60 (11%) Query: 101 AESVRRSLKLKELAPQINLLYKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWV 160 +++V + +++ A L + G+D FE E IE + D +E + PGW Sbjct: 778 SDAVHLPMAIRDRA-----LIERAFAGDDVAAEFEKEKAEIEAD--DDEKEIDNTLPGWG 830 >5MUU-D nrPDB Length = 149 Score = 28.9 bits (63), Expect = 1.5, Method: Composition-based stats. Identities = 10/42 (23%), Positives = 20/42 (47%) Query: 119 LLYKNGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWV 160 L + GS G D +++ + KD +A+ +QP ++ Sbjct: 55 TLAQIGSTGYDAYQQLLENHPEVAEMLKDLSFKADEIQPDFI 96 >5M6U-B nrPDB Length = 724 Score = 28.9 bits (63), Expect = 1.7, Method: Composition-based stats. Identities = 39/198 (19%), Positives = 80/198 (40%), Gaps = 30/198 (15%) Query: 9 FSNNGTFFETEEPI--VETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKISEQPSIF 66 FS+ TF E I +S++ Y P L V L+ S Y++ Q K ++ Sbjct: 392 FSDPLTFSSVVELINHYRNESLAQYNPK-----LDVKLLYPVSKYQQDQVVKEDNIEAVG 446 Query: 67 DENDAHDLYFQIKEMSENEKIHEKVLKAA----LLNRGAESVRRSLKLKELAPQINLLYK 122 + ++ FQ K E ++++E + + + E+ ++K+ E Q Y Sbjct: 447 KKLHEYNTQFQEKS-REYDRLYEDYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYS 505 Query: 123 NGSIGEDYWKRFETEVKLIELEFKDTLQEAERLQPGWVQLFVMVCKEICFNQALSRRYQS 182 +Y ++F+ E E E + + E+L+ ++ RR + Sbjct: 506 K-----EYIEKFKREGN--ETEIQRIMHNYEKLKSRISEIVDS-----------RRRLEE 547 Query: 183 ILKRKEVCIKEWELKINN 200 LK++ +E + ++N+ Sbjct: 548 DLKKQAAEYREIDKRMNS 565 >5Z79-A nrPDB Length = 717 Score = 28.5 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 12/43 (27%), Positives = 24/43 (55%), Gaps = 3/43 (6%) Query: 70 DAHDLYFQIKEMS---ENEKIHEKVLKAALLNRGAESVRRSLK 109 ++ DL ++ KE+ +NEKI+E +++ + RR +K Sbjct: 96 ESEDLIYECKELEVFLKNEKINESIIREVSVEDYENEARRIIK 138 >6GCS-5 nrPDB Length = 655 Score = 28.1 bits (61), Expect = 2.9, Method: Composition-based stats. Identities = 27/102 (26%), Positives = 42/102 (41%), Gaps = 4/102 (3%) Query: 11 NNGTFFETEEPIVETKSISVYTPLIYVFILVVSLVMFASSYRKKQAKKISEQPSIFDEND 70 NN ++F+TE I + + I V IL+V L F + K I+ SIF++ Sbjct: 495 NNFSYFDTEFSITQFYKLLPLISAILVSILIVVLNEFFAIVFNLNNKYINTVYSIFNQKL 554 Query: 71 AHDL----YFQIKEMSENEKIHEKVLKAALLNRGAESVRRSL 108 D + K + + I V K +L G + R L Sbjct: 555 VSDQILNHFIIFKGLVTSGNIAHHVDKGSLYRLGPVGINRLL 596 >5UIA-A nrPDB Length = 345 Score = 27.7 bits (60), Expect = 3.2, Method: Composition-based stats. Identities = 14/69 (20%), Positives = 29/69 (42%), Gaps = 14/69 (20%) Query: 80 EMSENEKIH--------EKVLKAALLNRGAES------VRRSLKLKELAPQINLLYKNGS 125 EM+ K+ + + A + R E+ V + + + + + ++G+ Sbjct: 85 EMAAAHKVPAICQKPFAKSLSDAKAMVRTCENADIPLMVHENFRWQTPIQAVKAVLESGA 144 Query: 126 IGEDYWKRF 134 IGE +W RF Sbjct: 145 IGEPFWGRF 153 >5OHN-A nrPDB Length = 370 Score = 27.7 bits (60), Expect = 3.7, Method: Composition-based stats. Identities = 17/64 (26%), Positives = 28/64 (43%), Gaps = 6/64 (9%) Query: 62 QPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALLNRGAESVRRSLKLKELAPQINLLY 121 Q S F+E DAH+L+ I E+E+ ++ + L V + E+ P+ Sbjct: 92 QISSFEEQDAHELFHVITSSLEDER--DRQPRVTHLF----DVHSLEQQSEITPKQITCR 145 Query: 122 KNGS 125 GS Sbjct: 146 TRGS 149 >3QNQ-A nrPDB Length = 442 Score = 27.3 bits (59), Expect = 5.4, Method: Composition-based stats. Identities = 12/31 (38%), Positives = 20/31 (64%) Query: 40 LVVSLVMFASSYRKKQAKKISEQPSIFDEND 70 LVV +++FA S + K ++S P IF+ N+ Sbjct: 304 LVVGMLLFARSQQLKSLGRLSIAPGIFNINE 334 >3OPB-A nrPDB Length = 778 Score = 27.0 bits (58), Expect = 5.6, Method: Composition-based stats. Identities = 12/50 (24%), Positives = 24/50 (48%), Gaps = 2/50 (4%) Query: 57 KKISEQPSIFDENDAHDLYFQIKEMSENEKIHEKVLKAALL--NRGAESV 104 K + P + NDA + FQ+++ SE+ K+ + + L + E + Sbjct: 30 KTLKSTPDVQKYNDAINTIFQLRQKSESGKMPADLTNSEALKDRQKIEEI 79 >3NZP-A nrPDB Length = 619 Score = 26.6 bits (57), Expect = 8.2, Method: Composition-based stats. Identities = 13/48 (27%), Positives = 24/48 (50%), Gaps = 2/48 (4%) Query: 44 LVMFASSYRKKQAKKISEQPSIFDENDAHDLYFQIKEMSENEKIHEKV 91 L +F+ Y + + + P + DE +DLY IK + E +H+ + Sbjct: 337 LELFSQEYAENKLILKKQNPKLIDE--LYDLYKSIKPSNALEYLHDSI 382 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.134 0.369 Lambda K H 0.267 0.0413 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,706,668 Number of Sequences: 36641 Number of extensions: 277016 Number of successful extensions: 849 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 837 Number of HSP's gapped (non-prelim): 31 length of query: 206 length of database: 10,336,785 effective HSP length: 92 effective length of query: 114 effective length of database: 6,965,813 effective search space: 794102682 effective search space used: 794102682 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.6 bits)