BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P33411 (251 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5MPS-a nrPDB 515 e-147 1DVK-A nrPDB 356 7e-99 5C18-A nrPDB 31 0.64 3HU1-A nrPDB 31 0.64 3DL9-A nrPDB 29 2.1 3LGZ-B nrPDB 28 3.2 5OF3-B nrPDB 28 4.2 5BPT-A nrPDB 27 6.5 1TLQ-A nrPDB 27 9.3 >5MPS-a nrPDB Length = 251 Score = 515 bits (1326), Expect = e-147, Method: Compositional matrix adjust. Identities = 251/251 (100%), Positives = 251/251 (100%) Query: 1 MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDETPRQVEQESTDEENLS 60 MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDETPRQVEQESTDEENLS Sbjct: 1 MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDETPRQVEQESTDEENLS 60 Query: 61 DNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEI 120 DNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEI Sbjct: 61 DNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEI 120 Query: 121 LSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAV 180 LSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAV Sbjct: 121 LSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAV 180 Query: 181 QSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFE 240 QSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFE Sbjct: 181 QSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFE 240 Query: 241 EWYTSNHDSLA 251 EWYTSNHDSLA Sbjct: 241 EWYTSNHDSLA 251 >1DVK-A nrPDB Length = 173 Score = 356 bits (913), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 172/172 (100%), Positives = 172/172 (100%) Query: 80 RIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDT 139 RIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDT Sbjct: 2 RIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDT 61 Query: 140 KKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTS 199 KKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTS Sbjct: 62 KKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTS 121 Query: 200 VGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFEEWYTSNHDSLA 251 VGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFEEWYTSNHDSLA Sbjct: 122 VGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFEEWYTSNHDSLA 173 >5C18-A nrPDB Length = 785 Score = 30.8 bits (68), Expect = 0.64, Method: Compositional matrix adjust. Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 2/40 (5%) Query: 60 SDNQSDDIRTTISKLENRPER--IQEAIAQDKTISVIIDP 97 +D++ DD+ T I K +NRP R + EAI +D ++ + P Sbjct: 4 ADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQP 43 >3HU1-A nrPDB Length = 489 Score = 30.8 bits (68), Expect = 0.64, Method: Compositional matrix adjust. Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 2/40 (5%) Query: 60 SDNQSDDIRTTISKLENRPER--IQEAIAQDKTISVIIDP 97 +D++ DD+ T I K +NRP R + EAI +D ++ + P Sbjct: 5 ADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQP 44 >3DL9-A nrPDB Length = 479 Score = 28.9 bits (63), Expect = 2.1, Method: Compositional matrix adjust. Identities = 27/77 (35%), Positives = 36/77 (46%), Gaps = 16/77 (20%) Query: 114 NLY-IHEILSRWKASLEAYHPELFLDT------KKALFPLLLQLRRNQLAPDL----LIS 162 NLY +H W+ E +HPE FLD+ K+AL P L RR+ L L + Sbjct: 377 NLYSVHFDEKYWRDP-EVFHPERFLDSSGYFAKKEALVPFSLG-RRHCLGEHLARMEMFL 434 Query: 163 LATVL---YHLQQPKEI 176 T L +HL P E+ Sbjct: 435 FFTALLQRFHLHFPHEL 451 >3LGZ-B nrPDB Length = 293 Score = 28.5 bits (62), Expect = 3.2, Method: Compositional matrix adjust. Identities = 16/58 (27%), Positives = 32/58 (55%), Gaps = 2/58 (3%) Query: 84 AIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDTKK 141 AIA+ K ++D ++ S E +P++ + +YI EIL + + H +F++ +K Sbjct: 223 AIAE-KDFRDVMDQIKVFSIEAQPIIELAARIYI-EILDEVRQANYTLHERVFVEKRK 278 >5OF3-B nrPDB Length = 307 Score = 28.1 bits (61), Expect = 4.2, Method: Compositional matrix adjust. Identities = 13/33 (39%), Positives = 17/33 (51%) Query: 12 EISKKKKELANSKGVQPPCTEKFQPHESANIDE 44 EI +K K L +G+ PPC E E N +E Sbjct: 198 EIPEKLKSLIERRGIIPPCIENILAKEKLNEEE 230 >5BPT-A nrPDB Length = 741 Score = 27.3 bits (59), Expect = 6.5, Method: Compositional matrix adjust. Identities = 16/47 (34%), Positives = 23/47 (48%) Query: 166 VLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQ 212 +LYHL + K + L Q Y L + A +T+VG AH +Q Sbjct: 352 LLYHLSELKGMALWRQKYEPLGLDAKAIEDAITAVGSFILKAHELLQ 398 >1TLQ-A nrPDB Length = 189 Score = 26.9 bits (58), Expect = 9.3, Method: Compositional matrix adjust. Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 2/68 (2%) Query: 134 ELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAW 193 E L+ ++ + +L L +QLA L L L HL + E + + LSI NV Sbjct: 54 EKVLNKREIIHAVLTGLALDQLAEQKL--LPEPLQHLVETDEPLYGIDEIIPLSIVNVYG 111 Query: 194 PIGVTSVG 201 IG+T+ G Sbjct: 112 SIGLTNFG 119 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5MPS-a nrPDB 531 e-151 1DVK-A nrPDB 367 e-102 Sequences not found previously or not previously below threshold: 5C18-A nrPDB 34 0.051 3HU1-A nrPDB 34 0.051 5ISU-A nrPDB 33 0.14 5OF3-B nrPDB 30 0.96 3LGZ-B nrPDB 29 2.2 1Z2Z-A nrPDB 29 2.6 1TLQ-A nrPDB 28 4.0 5BPT-A nrPDB 28 4.9 CONVERGED! >5MPS-a nrPDB Length = 251 Score = 531 bits (1367), Expect = e-151, Method: Composition-based stats. Identities = 251/251 (100%), Positives = 251/251 (100%) Query: 1 MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDETPRQVEQESTDEENLS 60 MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDETPRQVEQESTDEENLS Sbjct: 1 MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDETPRQVEQESTDEENLS 60 Query: 61 DNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEI 120 DNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEI Sbjct: 61 DNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEI 120 Query: 121 LSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAV 180 LSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAV Sbjct: 121 LSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAV 180 Query: 181 QSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFE 240 QSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFE Sbjct: 181 QSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFE 240 Query: 241 EWYTSNHDSLA 251 EWYTSNHDSLA Sbjct: 241 EWYTSNHDSLA 251 >1DVK-A nrPDB Length = 173 Score = 367 bits (943), Expect = e-102, Method: Composition-based stats. Identities = 172/172 (100%), Positives = 172/172 (100%) Query: 80 RIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDT 139 RIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDT Sbjct: 2 RIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDT 61 Query: 140 KKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTS 199 KKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTS Sbjct: 62 KKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTS 121 Query: 200 VGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFEEWYTSNHDSLA 251 VGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFEEWYTSNHDSLA Sbjct: 122 VGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFEEWYTSNHDSLA 173 >5C18-A nrPDB Length = 785 Score = 34.3 bits (77), Expect = 0.051, Method: Composition-based stats. Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 2/40 (5%) Query: 60 SDNQSDDIRTTISKLENRPER--IQEAIAQDKTISVIIDP 97 +D++ DD+ T I K +NRP R + EAI +D ++ + P Sbjct: 4 ADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQP 43 >3HU1-A nrPDB Length = 489 Score = 34.3 bits (77), Expect = 0.051, Method: Composition-based stats. Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 2/40 (5%) Query: 60 SDNQSDDIRTTISKLENRPER--IQEAIAQDKTISVIIDP 97 +D++ DD+ T I K +NRP R + EAI +D ++ + P Sbjct: 5 ADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQP 44 >5ISU-A nrPDB Length = 502 Score = 32.8 bits (73), Expect = 0.14, Method: Composition-based stats. Identities = 39/159 (24%), Positives = 59/159 (37%), Gaps = 17/159 (10%) Query: 22 NSKGVQPPCTEKFQPHESANIDETPRQVEQESTDEENLSDNQSDDIRTTISKLENRPERI 81 N K +QP K + + NI P +T EN +S ++R +S +R E I Sbjct: 230 NLKSIQPSDRNKVEKASAVNIITYPENRLSYATFNENQPALKSKELRQALSYALDREEII 289 Query: 82 QEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDTKK 141 A D+ P+ TE + K Y +I A + E DT + Sbjct: 290 DAAYGSDEYAK----PASSFLTENTKYFTDKVETYDQDI-----AKAKKLVKESGFDTSQ 340 Query: 142 ALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAV 180 L L ++Q + LY QQ KEI + + Sbjct: 341 KLTVYYLNNSKSQ--------ESIALYLQQQYKEIGVTL 371 >5OF3-B nrPDB Length = 307 Score = 30.1 bits (66), Expect = 0.96, Method: Composition-based stats. Identities = 13/33 (39%), Positives = 17/33 (51%) Query: 12 EISKKKKELANSKGVQPPCTEKFQPHESANIDE 44 EI +K K L +G+ PPC E E N +E Sbjct: 198 EIPEKLKSLIERRGIIPPCIENILAKEKLNEEE 230 >3LGZ-B nrPDB Length = 293 Score = 28.9 bits (63), Expect = 2.2, Method: Composition-based stats. Identities = 16/58 (27%), Positives = 32/58 (55%), Gaps = 2/58 (3%) Query: 84 AIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDTKK 141 AIA+ K ++D ++ S E +P++ + +YI EIL + + H +F++ +K Sbjct: 223 AIAE-KDFRDVMDQIKVFSIEAQPIIELAARIYI-EILDEVRQANYTLHERVFVEKRK 278 >1Z2Z-A nrPDB Length = 446 Score = 28.5 bits (62), Expect = 2.6, Method: Composition-based stats. Identities = 24/97 (24%), Positives = 44/97 (45%), Gaps = 10/97 (10%) Query: 5 LASILKGEISKKKKELANSKGVQPPCTEKFQPH-------ESANIDETPRQVEQESTDEE 57 L+ IL ++S+K+ +A +K + T+K E ++ + +V S Sbjct: 68 LSRIL--QVSQKRISVAGTKDKRALTTQKISIFDTDASEIEKIHLKDIELKVLGRSRKSV 125 Query: 58 NLSDNQSDDIRTTISKLENRPERIQEAIAQDKTISVI 94 L D +D R T+ +EN PE EA+ + T ++ Sbjct: 126 ELGDLWGNDFRITVRNIENSPEE-TEALLKKTTDEIL 161 >1TLQ-A nrPDB Length = 189 Score = 28.1 bits (61), Expect = 4.0, Method: Composition-based stats. Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 2/68 (2%) Query: 134 ELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAW 193 E L+ ++ + +L L +QLA L L L HL + E + + LSI NV Sbjct: 54 EKVLNKREIIHAVLTGLALDQLAEQKL--LPEPLQHLVETDEPLYGIDEIIPLSIVNVYG 111 Query: 194 PIGVTSVG 201 IG+T+ G Sbjct: 112 SIGLTNFG 119 >5BPT-A nrPDB Length = 741 Score = 27.8 bits (60), Expect = 4.9, Method: Composition-based stats. Identities = 16/47 (34%), Positives = 23/47 (48%) Query: 166 VLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQ 212 +LYHL + K + L Q Y L + A +T+VG AH +Q Sbjct: 352 LLYHLSELKGMALWRQKYEPLGLDAKAIEDAITAVGSFILKAHELLQ 398 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.130 0.369 Lambda K H 0.267 0.0407 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,901,745 Number of Sequences: 36641 Number of extensions: 323778 Number of successful extensions: 784 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 770 Number of HSP's gapped (non-prelim): 32 length of query: 251 length of database: 10,336,785 effective HSP length: 95 effective length of query: 156 effective length of database: 6,855,890 effective search space: 1069518840 effective search space used: 1069518840 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 58 (27.0 bits)