BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P32914 (102 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2EXU-A nrPDB 211 4e-56 6J4Z-V nrPDB 105 4e-24 6GML-Y nrPDB 89 6e-19 4ZN3-B nrPDB 27 2.2 5NYY-A nrPDB 26 4.2 1CP9-B nrPDB 25 5.8 2NZA-A nrPDB 25 6.9 4IQR-A nrPDB 25 7.1 1KB2-A nrPDB 25 8.6 >2EXU-A nrPDB Length = 200 Score = 211 bits (538), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 99/99 (100%), Positives = 99/99 (100%) Query: 1 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWV 60 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWV Sbjct: 1 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWV 60 Query: 61 AKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGS 99 AKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGS Sbjct: 61 AKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGS 99 >6J4Z-V nrPDB Length = 108 Score = 105 bits (262), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 52/106 (49%), Positives = 68/106 (64%), Gaps = 7/106 (6%) Query: 4 ERACMLCGIVQTTNEFNRDGCPNCQGIF---EEAGVSTMECTSPSFEGLVGMCKPTKSWV 60 ERACMLCGIV F ++GCPNC + + + ECTS SFEGLV + SWV Sbjct: 3 ERACMLCGIVLPGRVFMQNGCPNCDSVLNLRDSDQATVNECTSSSFEGLVAVGDNEHSWV 62 Query: 61 AKWLSVDHSIAGMYAIKVDGRLPAEVVELLP----HYKPRDGSQVE 102 AKWL VD G+YA++VDGRLP+++V L +Y+PRDGS ++ Sbjct: 63 AKWLRVDRFQPGLYAVRVDGRLPSDIVAALEQYGVYYRPRDGSVID 108 >6GML-Y nrPDB Length = 121 Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 43/99 (43%), Positives = 58/99 (58%), Gaps = 6/99 (6%) Query: 5 RACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTM--ECTSPSFEGLVGMCKPTKSWVAK 62 RAC+LC +V+T ++F DGC NC + G M +CTS SF+G++ M P SWV+K Sbjct: 18 RACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSK 77 Query: 63 WLSVDHSIAGMYAIKVDGRLPAEVVELLPH----YKPRD 97 W V + G+YA+ V GRLP +V L YK RD Sbjct: 78 WQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRD 116 >4ZN3-B nrPDB Length = 72 Score = 26.9 bits (58), Expect = 2.2, Method: Compositional matrix adjust. Identities = 23/74 (31%), Positives = 33/74 (44%), Gaps = 18/74 (24%) Query: 5 RACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWVAKWL 64 RAC+ C + N + CP C TS ++ GL+ + P KS +AK Sbjct: 15 RACLKCKYLT-----NDEICPICHSP-----------TSENWIGLLIVINPEKSEIAKKA 58 Query: 65 SVDHSIAGMYAIKV 78 +D I G YA+ V Sbjct: 59 GID--IKGKYALSV 70 >5NYY-A nrPDB Length = 300 Score = 25.8 bits (55), Expect = 4.2, Method: Compositional matrix adjust. Identities = 13/49 (26%), Positives = 20/49 (40%) Query: 35 GVSTMECTSPSFEGLVGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGRLP 83 G + + +P+ GL + W A W S D A ++D R P Sbjct: 203 GTAPVNAFAPNGYGLYNVAGNVWEWCADWWSADWHATESPATRIDPRGP 251 >1CP9-B nrPDB Length = 553 Score = 25.4 bits (54), Expect = 5.8, Method: Composition-based stats. Identities = 11/34 (32%), Positives = 17/34 (50%) Query: 17 NEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLV 50 NE++ G N +F E GVS + +P G + Sbjct: 474 NEYHNRGTENDLIVFTEEGVSAWDVVAPGQSGFI 507 >2NZA-A nrPDB Length = 413 Score = 25.0 bits (53), Expect = 6.9, Method: Composition-based stats. Identities = 8/15 (53%), Positives = 11/15 (73%) Query: 85 EVVELLPHYKPRDGS 99 ++ L PH+KPR GS Sbjct: 81 QITRLAPHFKPRPGS 95 >4IQR-A nrPDB Length = 338 Score = 25.0 bits (53), Expect = 7.1, Method: Composition-based stats. Identities = 9/34 (26%), Positives = 13/34 (38%) Query: 1 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEA 34 M C +CG T + C C+G F + Sbjct: 15 MGVSALCAICGDRATGKHYGASSCDGCKGFFRRS 48 >1KB2-A nrPDB Length = 110 Score = 25.0 bits (53), Expect = 8.6, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 21/54 (38%), Gaps = 4/54 (7%) Query: 5 RACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKS 58 R C +CG T FN C C+G F + T P G C+ TK Sbjct: 7 RICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCP----FNGDCRITKD 56 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2EXU-A nrPDB 189 2e-49 6J4Z-V nrPDB 165 4e-42 6GML-Y nrPDB 154 1e-38 Sequences not found previously or not previously below threshold: 4ZN3-B nrPDB 30 0.31 6I2N-D nrPDB 29 0.42 5FSG-A nrPDB 29 0.42 3BXW-B nrPDB 29 0.48 3J7A-D nrPDB 28 0.98 1DXS-A nrPDB 27 1.5 1COK-A nrPDB 27 1.5 5XXU-D nrPDB 27 1.5 3IT8-D nrPDB 27 2.3 2DJ7-A nrPDB 27 2.9 4FE3-A nrPDB 26 3.2 1LO1-A nrPDB 26 3.4 5E04-A nrPDB 26 3.9 5E05-A nrPDB 26 4.4 5XJX-C nrPDB 26 4.7 4XIJ-A nrPDB 25 6.0 5LZT-jj nrPDB 25 7.4 3P2F-A nrPDB 25 7.9 5KRB-B nrPDB 25 9.8 CONVERGED! >2EXU-A nrPDB Length = 200 Score = 189 bits (480), Expect = 2e-49, Method: Composition-based stats. Identities = 99/99 (100%), Positives = 99/99 (100%) Query: 1 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWV 60 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWV Sbjct: 1 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWV 60 Query: 61 AKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGS 99 AKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGS Sbjct: 61 AKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGS 99 >6J4Z-V nrPDB Length = 108 Score = 165 bits (418), Expect = 4e-42, Method: Composition-based stats. Identities = 52/106 (49%), Positives = 68/106 (64%), Gaps = 7/106 (6%) Query: 4 ERACMLCGIVQTTNEFNRDGCPNCQGIF---EEAGVSTMECTSPSFEGLVGMCKPTKSWV 60 ERACMLCGIV F ++GCPNC + + + ECTS SFEGLV + SWV Sbjct: 3 ERACMLCGIVLPGRVFMQNGCPNCDSVLNLRDSDQATVNECTSSSFEGLVAVGDNEHSWV 62 Query: 61 AKWLSVDHSIAGMYAIKVDGRLPAEVVELLP----HYKPRDGSQVE 102 AKWL VD G+YA++VDGRLP+++V L +Y+PRDGS ++ Sbjct: 63 AKWLRVDRFQPGLYAVRVDGRLPSDIVAALEQYGVYYRPRDGSVID 108 >6GML-Y nrPDB Length = 121 Score = 154 bits (388), Expect = 1e-38, Method: Composition-based stats. Identities = 43/102 (42%), Positives = 59/102 (57%), Gaps = 6/102 (5%) Query: 5 RACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTM--ECTSPSFEGLVGMCKPTKSWVAK 62 RAC+LC +V+T ++F DGC NC + G M +CTS SF+G++ M P SWV+K Sbjct: 18 RACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSK 77 Query: 63 WLSVDHSIAGMYAIKVDGRLPAEVVELLPH----YKPRDGSQ 100 W V + G+YA+ V GRLP +V L YK RD + Sbjct: 78 WQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAI 119 >4ZN3-B nrPDB Length = 72 Score = 29.7 bits (65), Expect = 0.31, Method: Composition-based stats. Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 18/74 (24%) Query: 5 RACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWVAKWL 64 RAC+ C T +E CP C TS ++ GL+ + P KS +AK Sbjct: 15 RACLKCK-YLTNDEI----CPICHSP-----------TSENWIGLLIVINPEKSEIAKKA 58 Query: 65 SVDHSIAGMYAIKV 78 +D I G YA+ V Sbjct: 59 GID--IKGKYALSV 70 >6I2N-D nrPDB Length = 430 Score = 29.3 bits (64), Expect = 0.42, Method: Composition-based stats. Identities = 14/60 (23%), Positives = 23/60 (38%), Gaps = 5/60 (8%) Query: 32 EEAGVSTMECTSPSFEGLVGMCKPTKSWVA-----KWLSVDHSIAGMYAIKVDGRLPAEV 86 + G SFE + G+ KP +V+ + + G Y V G PA++ Sbjct: 152 DNKGTRIRFKDDSSFEDVNGIRKPKHLYVSLPNAQSSMKAEEITPGRYRTAVCGLYPAQI 211 >5FSG-A nrPDB Length = 698 Score = 29.3 bits (64), Expect = 0.42, Method: Composition-based stats. Identities = 14/60 (23%), Positives = 23/60 (38%), Gaps = 5/60 (8%) Query: 32 EEAGVSTMECTSPSFEGLVGMCKPTKSWVA-----KWLSVDHSIAGMYAIKVDGRLPAEV 86 + G SFE + G+ KP +V+ + + G Y V G PA++ Sbjct: 412 DNKGTRIRFKDDSSFEDVNGIRKPKHLYVSLPNANSSMKAEEITPGRYRTAVCGLYPAQI 471 >3BXW-B nrPDB Length = 393 Score = 28.9 bits (63), Expect = 0.48, Method: Composition-based stats. Identities = 14/50 (28%), Positives = 21/50 (42%), Gaps = 3/50 (6%) Query: 50 VGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGRLP---AEVVELLPHYKPR 96 V + P W +K L + YA D R P A ++ L ++PR Sbjct: 282 VQVLDPKSKWRSKILLGLNFYGMDYATSKDAREPVVGARYIQTLKDHRPR 331 >3J7A-D nrPDB Length = 221 Score = 28.1 bits (61), Expect = 0.98, Method: Composition-based stats. Identities = 20/88 (22%), Positives = 33/88 (37%), Gaps = 8/88 (9%) Query: 1 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSF-EG-LVGMCKPTKS 58 ++ RAC G++ G C+ I + S F +G L+ +P+K Sbjct: 114 LAVRRACY--GVL---RHIMESGAKGCEVIV-SGKLRAQRAKSMKFRDGYLISTGEPSKR 167 Query: 59 WVAKWLSVDHSIAGMYAIKVDGRLPAEV 86 +V G+ IKV LP + Sbjct: 168 FVNTATRSAQLKQGVLGIKVKIMLPTAI 195 >1DXS-A nrPDB Length = 80 Score = 27.4 bits (59), Expect = 1.5, Method: Composition-based stats. Identities = 13/51 (25%), Positives = 19/51 (37%), Gaps = 1/51 (1%) Query: 23 GCPNCQGIFEEAG-VSTMECTSPSFEGLVGMCKPTKSWVAKWLSVDHSIAG 72 GCPNC F G S + + E L + P + + W + G Sbjct: 17 GCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQG 67 >1COK-A nrPDB Length = 68 Score = 27.4 bits (59), Expect = 1.5, Method: Composition-based stats. Identities = 13/51 (25%), Positives = 19/51 (37%), Gaps = 1/51 (1%) Query: 23 GCPNCQGIFEEAG-VSTMECTSPSFEGLVGMCKPTKSWVAKWLSVDHSIAG 72 GCPNC F G S + + E L + P + + W + G Sbjct: 15 GCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQG 65 >5XXU-D nrPDB Length = 235 Score = 27.4 bits (59), Expect = 1.5, Method: Composition-based stats. Identities = 17/87 (19%), Positives = 35/87 (40%), Gaps = 8/87 (9%) Query: 1 MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSF-EG-LVGMCKPTKS 58 ++ RAC G++ G C+ + + S F +G L+ +P+K Sbjct: 115 LAVRRACY--GVL---RHIMESGAKGCEVVV-SGKLRAQRAKSMKFKDGYLISTGEPSKM 168 Query: 59 WVAKWLSVDHSIAGMYAIKVDGRLPAE 85 +V + + G+ ++V LP + Sbjct: 169 FVDQAIRSVQLRQGVLGVRVKIMLPHD 195 >3IT8-D nrPDB Length = 324 Score = 26.6 bits (57), Expect = 2.3, Method: Composition-based stats. Identities = 17/49 (34%), Positives = 22/49 (44%), Gaps = 10/49 (20%) Query: 33 EAGVSTMECTSPSFEGLVGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGR 81 E G T+ECT SF P + KW+ +H G Y V+GR Sbjct: 194 EDGNMTLECTVNSF-------YPPDV-ITKWIESEHFK-GEYKY-VNGR 232 >2DJ7-A nrPDB Length = 80 Score = 26.6 bits (57), Expect = 2.9, Method: Composition-based stats. Identities = 7/23 (30%), Positives = 12/23 (52%) Query: 7 CMLCGIVQTTNEFNRDGCPNCQG 29 C C ++ T ++DG P C+ Sbjct: 45 CQTCSVILTGEYISKDGVPYCES 67 >4FE3-A nrPDB Length = 297 Score = 26.2 bits (56), Expect = 3.2, Method: Composition-based stats. Identities = 12/50 (24%), Positives = 22/50 (44%), Gaps = 16/50 (32%) Query: 8 MLCGIVQ--------------TTNEFNRDG--CPNCQGIFEEAGVSTMEC 41 ++CG+++ T + F+ +G CP C I + + T EC Sbjct: 32 IICGLIKGGAAKLQIITDFNMTLSRFSYNGKRCPTCHNIIDNCKLVTDEC 81 >1LO1-A nrPDB Length = 98 Score = 26.2 bits (56), Expect = 3.4, Method: Composition-based stats. Identities = 9/44 (20%), Positives = 19/44 (43%), Gaps = 2/44 (4%) Query: 4 ERACMLCGIVQTTNEFNRDGCPNCQGIFEE--AGVSTMECTSPS 45 +R C++CG + + + C C+ F+ G C + + Sbjct: 4 KRLCLVCGDIASGYHYGVASCEACKAFFKRTIQGNIEYSCPATN 47 >5E04-A nrPDB Length = 284 Score = 25.8 bits (55), Expect = 3.9, Method: Composition-based stats. Identities = 14/64 (21%), Positives = 24/64 (37%), Gaps = 5/64 (7%) Query: 28 QGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWVA-----KWLSVDHSIAGMYAIKVDGRL 82 Q + + G SFE + G+ KP +V+ + + G + G Sbjct: 32 QTVKDNKGTRIRFKDDSSFEEVNGIRKPKHLYVSMPTAQSTMKAEEITPGRFRTIACGLF 91 Query: 83 PAEV 86 PA+V Sbjct: 92 PAQV 95 >5E05-A nrPDB Length = 284 Score = 25.8 bits (55), Expect = 4.4, Method: Composition-based stats. Identities = 16/64 (25%), Positives = 24/64 (37%), Gaps = 5/64 (7%) Query: 28 QGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWVA-----KWLSVDHSIAGMYAIKVDGRL 82 Q I E G S+E + G+ KP +V+ + D G + G Sbjct: 34 QTIKENKGTRIRFKDDSSYEEVNGIRKPRHLYVSMPTAQSTMKADEITPGRFRTIACGLF 93 Query: 83 PAEV 86 PA+V Sbjct: 94 PAQV 97 >5XJX-C nrPDB Length = 433 Score = 25.8 bits (55), Expect = 4.7, Method: Composition-based stats. Identities = 16/74 (21%), Positives = 30/74 (40%), Gaps = 9/74 (12%) Query: 34 AGVSTMECTSPSFEGL-----VGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGRLPAEVVE 88 + S SF L C P +S+V++ L+ + ++ + GR P + Sbjct: 66 DQDNVYHVVSLSFGALSDDTAFPTCDPQRSYVSESLTRLKHLKALFFYRCLGRAPQRI-- 123 Query: 89 LLPHYKPRDGSQVE 102 P + R GS ++ Sbjct: 124 --PAFLGRLGSSLQ 135 >4XIJ-A nrPDB Length = 275 Score = 25.4 bits (54), Expect = 6.0, Method: Composition-based stats. Identities = 10/37 (27%), Positives = 17/37 (45%), Gaps = 6/37 (16%) Query: 39 MECTSPSFEGLVGMCKPTKSWVAKWLSVDHSIAGMYA 75 +ECT+ GLV P W+ + ++ G +A Sbjct: 49 IECTAEQLPGLVSALGPE------WVGLSVTMPGKFA 79 >5LZT-jj nrPDB Length = 637 Score = 25.1 bits (53), Expect = 7.4, Method: Composition-based stats. Identities = 15/57 (26%), Positives = 23/57 (40%), Gaps = 5/57 (8%) Query: 27 CQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGRLP 83 C+G +ME + P E P +SW K + + G ++ DGR P Sbjct: 130 CEG---SNSAVSMELSEPIVENGETEMSPEESWEHK-EEISEAEPGGGSLG-DGRPP 181 >3P2F-A nrPDB Length = 201 Score = 25.1 bits (53), Expect = 7.9, Method: Composition-based stats. Identities = 8/17 (47%), Positives = 10/17 (58%) Query: 80 GRLPAEVVELLPHYKPR 96 GRLPA + L Y+ R Sbjct: 9 GRLPAHIAAELGSYRYR 25 >5KRB-B nrPDB Length = 84 Score = 24.7 bits (52), Expect = 9.8, Method: Composition-based stats. Identities = 9/29 (31%), Positives = 15/29 (51%) Query: 4 ERACMLCGIVQTTNEFNRDGCPNCQGIFE 32 +R C++CG T + C C+G F+ Sbjct: 1 QRTCLICGDRATGLHYGIISCEGCKGFFK 29 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.131 0.404 Lambda K H 0.267 0.0408 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,248,872 Number of Sequences: 36641 Number of extensions: 149706 Number of successful extensions: 394 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 22 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 366 Number of HSP's gapped (non-prelim): 38 length of query: 102 length of database: 10,336,785 effective HSP length: 69 effective length of query: 33 effective length of database: 7,808,556 effective search space: 257682348 effective search space used: 257682348 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 52 (24.6 bits)