BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P32910 (317 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6F42-P nrPDB 656 0.0 2DK5-A nrPDB 65 6e-11 2YU3-A nrPDB 55 3e-08 3I5J-B nrPDB 30 1.8 5OWV-A nrPDB 28 6.1 5VAF-A nrPDB 28 6.1 1CYD-A nrPDB 28 6.1 >6F42-P nrPDB Length = 317 Score = 656 bits (1693), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/317 (100%), Positives = 317/317 (100%) Query: 1 MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTDLMSIVQELLDKNL 60 MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTDLMSIVQELLDKNL Sbjct: 1 MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTDLMSIVQELLDKNL 60 Query: 61 IKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHV 120 IKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHV Sbjct: 61 IKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHV 120 Query: 121 VLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLT 180 VLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLT Sbjct: 121 VLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLT 180 Query: 181 IVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSNI 240 IVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSNI Sbjct: 181 IVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSNI 240 Query: 241 RSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEGEPEAGNKALEDEEEFSIFNYFKMF 300 RSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEGEPEAGNKALEDEEEFSIFNYFKMF Sbjct: 241 RSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEGEPEAGNKALEDEEEFSIFNYFKMF 300 Query: 301 PASKHDKEVVYFDEWTI 317 PASKHDKEVVYFDEWTI Sbjct: 301 PASKHDKEVVYFDEWTI 317 >2DK5-A nrPDB Length = 91 Score = 64.7 bits (156), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 32/69 (46%), Positives = 49/69 (71%), Gaps = 1/69 (1%) Query: 89 EEALVYSYIEASGREGIWSKTIKARTNLHQHVVLKCLKSLESQRYVKSVKSVKFPTRKIY 148 +E LVY IE +G +GIWS+ ++ ++NL + K LK+LES++ +K+VKSV +K+Y Sbjct: 21 QEKLVYQIIEDAGNKGIWSRDVRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVY 80 Query: 149 MLYSLQ-PS 156 MLY+L PS Sbjct: 81 MLYNLSGPS 89 >2YU3-A nrPDB Length = 95 Score = 55.5 bits (132), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 30/84 (35%), Positives = 50/84 (59%) Query: 61 IKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHV 120 + L++ N L ++ A K +E LVY IE +G +GIWS+ ++ ++NL Sbjct: 10 LDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDVRYKSNLPLTE 69 Query: 121 VLKCLKSLESQRYVKSVKSVKFPT 144 + K LK+LES++ +K+VKSV P+ Sbjct: 70 INKILKNLESKKLIKAVKSVSGPS 93 >3I5J-B nrPDB Length = 327 Score = 29.6 bits (65), Expect = 1.8, Method: Compositional matrix adjust. Identities = 22/98 (22%), Positives = 43/98 (43%), Gaps = 9/98 (9%) Query: 212 VKNYSTTQEILEFITAAQVANVELTPSNIRSLCEVLVYDDKLEKVTHDCYRVTLESILQM 271 + YS + + + + ++ P++ S C +L D L +TH YR S+ Sbjct: 119 ARCYSPLRYLFHCLQMSSAYVQQMAPASTISNCCILQTADSLRWLTHTAYRTHELSLTYP 178 Query: 272 NQGEG-------EPEAGNKALED--EEEFSIFNYFKMF 300 + G G E E G + L + E++ + F++ + F Sbjct: 179 DAGLGEHERELWEKEPGWQGLRELMEKQLTAFDWGEAF 216 >5OWV-A nrPDB Length = 732 Score = 27.7 bits (60), Expect = 6.1, Method: Compositional matrix adjust. Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 9/58 (15%) Query: 157 VDITGGPWFTDGELDIEFIN--------SLLTIVWRFISENTFPNGFKNFENGPKKNV 206 VDIT D +I+F N + L++ + F+ ++ F NGF+ F++G KN+ Sbjct: 552 VDITTKDGINDILREIKFENIKKIEELKTNLSLKYDFLKDD-FDNGFEGFKDGISKNI 608 >5VAF-A nrPDB Length = 533 Score = 27.7 bits (60), Expect = 6.1, Method: Compositional matrix adjust. Identities = 16/40 (40%), Positives = 24/40 (60%) Query: 34 QELQKQMGIGSLTDLMSIVQELLDKNLIKLVKQNDELKFQ 73 QEL+ + I ++TD +S++QEL D LI + Q L Q Sbjct: 405 QELEYRFRIRNITDELSLIQELDDTRLIIDLSQQPNLYTQ 444 >1CYD-A nrPDB Length = 244 Score = 27.7 bits (60), Expect = 6.1, Method: Compositional matrix adjust. Identities = 27/112 (24%), Positives = 50/112 (44%), Gaps = 6/112 (5%) Query: 150 LYSLQPSVDITGGPWFTDGELDIEFINSLLTIVWRFISENTFPNGFKNFENGPKKNVFYA 209 L +QP +++T + +++ + + +V R + P N + F Sbjct: 86 LVIMQPFLEVTKEAFDRSFSVNLRSVFQVSQMVARDMINRGVPGSIVNVSSMVAHVTF-- 143 Query: 210 PNVKNYSTTQEILEFITAAQVANVELTPSNIR--SLCEVLVYDDKLEKVTHD 259 PN+ YS+T+ + +T A +EL P IR S+ +V D +KV+ D Sbjct: 144 PNLITYSSTKGAMTMLTKAMA--MELGPHKIRVNSVNPTVVLTDMGKKVSAD 193 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6F42-P nrPDB 630 0.0 2YU3-A nrPDB 142 2e-34 2DK5-A nrPDB 132 2e-31 Sequences not found previously or not previously below threshold: 2DK8-A nrPDB 34 0.096 1FV2-A nrPDB 31 0.55 3MPP-G nrPDB 31 0.71 2VXR-A nrPDB 31 0.71 6G5K-A nrPDB 31 0.83 1G01-A nrPDB 30 0.94 3EUK-L nrPDB 30 1.0 5VAF-A nrPDB 30 1.1 3I5J-B nrPDB 29 4.0 5YTI-A nrPDB 29 4.1 2XD3-A nrPDB 28 5.1 CONVERGED! >6F42-P nrPDB Length = 317 Score = 630 bits (1626), Expect = 0.0, Method: Composition-based stats. Identities = 317/317 (100%), Positives = 317/317 (100%) Query: 1 MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTDLMSIVQELLDKNL 60 MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTDLMSIVQELLDKNL Sbjct: 1 MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTDLMSIVQELLDKNL 60 Query: 61 IKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHV 120 IKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHV Sbjct: 61 IKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHV 120 Query: 121 VLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLT 180 VLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLT Sbjct: 121 VLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLT 180 Query: 181 IVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSNI 240 IVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSNI Sbjct: 181 IVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSNI 240 Query: 241 RSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEGEPEAGNKALEDEEEFSIFNYFKMF 300 RSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEGEPEAGNKALEDEEEFSIFNYFKMF Sbjct: 241 RSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEGEPEAGNKALEDEEEFSIFNYFKMF 300 Query: 301 PASKHDKEVVYFDEWTI 317 PASKHDKEVVYFDEWTI Sbjct: 301 PASKHDKEVVYFDEWTI 317 >2YU3-A nrPDB Length = 95 Score = 142 bits (358), Expect = 2e-34, Method: Composition-based stats. Identities = 30/84 (35%), Positives = 50/84 (59%) Query: 61 IKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHV 120 + L++ N L ++ A K +E LVY IE +G +GIWS+ ++ ++NL Sbjct: 10 LDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDVRYKSNLPLTE 69 Query: 121 VLKCLKSLESQRYVKSVKSVKFPT 144 + K LK+LES++ +K+VKSV P+ Sbjct: 70 INKILKNLESKKLIKAVKSVSGPS 93 >2DK5-A nrPDB Length = 91 Score = 132 bits (332), Expect = 2e-31, Method: Composition-based stats. Identities = 32/77 (41%), Positives = 49/77 (63%) Query: 78 SEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHVVLKCLKSLESQRYVKSV 137 A K +E LVY IE +G +GIWS+ ++ ++NL + K LK+LES++ +K+V Sbjct: 10 QNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDVRYKSNLPLTEINKILKNLESKKLIKAV 69 Query: 138 KSVKFPTRKIYMLYSLQ 154 KSV +K+YMLY+L Sbjct: 70 KSVAASKKKVYMLYNLS 86 >2DK8-A nrPDB Length = 81 Score = 33.9 bits (76), Expect = 0.096, Method: Composition-based stats. Identities = 9/46 (19%), Positives = 17/46 (36%) Query: 32 TQQELQKQMGIGSLTDLMSIVQELLDKNLIKLVKQNDELKFQGVLE 77 T Q +Q +M + LL + L++ N L ++ Sbjct: 31 TDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDS 76 >1FV2-A nrPDB Length = 472 Score = 31.2 bits (69), Expect = 0.55, Method: Composition-based stats. Identities = 33/155 (21%), Positives = 61/155 (39%), Gaps = 28/155 (18%) Query: 40 MGIGSLTDLMSIVQELLDKNL-IKLVKQNDELKFQGVLESEAQKKATMSAE-EALVYSYI 97 MG +T L +I + D N+ +KL + N+ ++ + + KA E E L SY+ Sbjct: 210 MGSAEITGLGAIRE---DNNITLKLDRCNNNNQYVSIDKFRIFCKALNPKEIEKLYTSYL 266 Query: 98 EASGREGIWSKTIKARTNLHQHVVLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSV 157 + W ++ T + V + K L ++ + Sbjct: 267 SITFLRDFWGNPLRYDTEYYLIPV--------------------ASSSKDVQLKNITDYM 306 Query: 158 DITGGPWFTDGELDIEF---INSLLTIVWRFISEN 189 +T P +T+G+L+I + N L I+ R+ N Sbjct: 307 YLTNAPSYTNGKLNIYYRRLYNGLKFIIKRYTPNN 341 >3MPP-G nrPDB Length = 433 Score = 30.8 bits (68), Expect = 0.71, Method: Composition-based stats. Identities = 19/107 (17%), Positives = 42/107 (39%), Gaps = 13/107 (12%) Query: 73 QGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQH------VVLKCLK 126 Q L + T + +VY + + W +T K N Q ++ C+K Sbjct: 39 QFKLNNSENSNITAHQSKFVVYDSMFDNFSINFWVRTPKYNNNDIQTYLQNEYTIISCIK 98 Query: 127 S-------LESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFT 166 + ++ R + ++ V ++ I+ YS++ ++ WF+ Sbjct: 99 NDSGWKVSIKGNRIIWTLIDVNAKSKSIFFEYSIKDNISDYINKWFS 145 >2VXR-A nrPDB Length = 482 Score = 30.8 bits (68), Expect = 0.71, Method: Composition-based stats. Identities = 19/107 (17%), Positives = 42/107 (39%), Gaps = 13/107 (12%) Query: 73 QGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQH------VVLKCLK 126 Q L + T + +VY + + W +T K N Q ++ C+K Sbjct: 88 QFKLNNSENSNITAHQSKFVVYDSMFDNFSINFWVRTPKYNNNDIQTYLQNEYTIISCIK 147 Query: 127 S-------LESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFT 166 + ++ R + ++ V ++ I+ YS++ ++ WF+ Sbjct: 148 NDSGWKVSIKGNRIIWTLIDVNAKSKSIFFEYSIKDNISDYINKWFS 194 >6G5K-A nrPDB Length = 461 Score = 30.8 bits (68), Expect = 0.83, Method: Composition-based stats. Identities = 41/233 (17%), Positives = 90/233 (38%), Gaps = 54/233 (23%) Query: 57 DKNLIKL------VKQNDEL----KFQGVLESEAQKKATMSAEEALVYSYIEASGREGIW 106 D NLI L V+ D + K Q L S A K ++ + ++++ + W Sbjct: 40 DNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIRVTQNQNIIFNSVFLDFSVSFW 99 Query: 107 SKTIKARTN------LHQHVVLKCLKS-------LESQRYVKSVKSVKFPTRKIYMLYSL 153 + K + + +++ ++ C+K+ + R + ++ + T+ ++ Y++ Sbjct: 100 IRIPKYKNDGIQNYIHNEYTIINCMKNNSGWKISIRGNRIIWTLIDINGKTKSVFFEYNI 159 Query: 154 QPSVDITGGPWFTDGELDIEFINSLLTIVWRFISENTFPNGFKNFENGPKKNVFYAPNVK 213 + + E+IN W F++ N K + NG ++ +++ Sbjct: 160 REDIS--------------EYINR-----WFFVTITNNLNNAKIYINGKLESNTDIKDIR 200 Query: 214 NYSTTQEIL----------EFITAA--QVANVELTPSNIRSLCEVLVYDDKLE 254 EI+ +FI + N EL+ SNI ++ Y + L+ Sbjct: 201 EVIANGEIIFKLDGDIDRTQFIWMKYFSIFNTELSQSNIEERYKIQSYSEYLK 253 >1G01-A nrPDB Length = 364 Score = 30.4 bits (67), Expect = 0.94, Method: Composition-based stats. Identities = 22/87 (25%), Positives = 39/87 (44%), Gaps = 16/87 (18%) Query: 161 GGPWFTDGELDIEFIN--SLLTIVWRFISENTFPNGFKNFENG--------PKKNVFYAP 210 GGP+F + ++ + F+N ++ W ++N F FE G P N +AP Sbjct: 275 GGPYFDEADVWLNFLNKHNISWANWSLTNKNEISGAFTPFELGRTDATDLDPGANQVWAP 334 Query: 211 NVKNYSTTQEILEFITAAQVANVELTP 237 + S + E + A++ +E TP Sbjct: 335 --EELSLSGEYVR----ARIKGIEYTP 355 >3EUK-L nrPDB Length = 238 Score = 30.4 bits (67), Expect = 1.0, Method: Composition-based stats. Identities = 17/94 (18%), Positives = 37/94 (39%), Gaps = 7/94 (7%) Query: 45 LTDLMSIVQELLDKNLIKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYI------- 97 L D + ++ + + L++ + + S ++ MS E LV + Sbjct: 46 LMDFQTELESFYRRYHVDLIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLYLSP 105 Query: 98 EASGREGIWSKTIKARTNLHQHVVLKCLKSLESQ 131 E ++GI+S+ L+ K LK++ + Sbjct: 106 ERLAQQGIFSQDDVYEELLNLADENKLLKAVNPR 139 >5VAF-A nrPDB Length = 533 Score = 30.4 bits (67), Expect = 1.1, Method: Composition-based stats. Identities = 16/41 (39%), Positives = 24/41 (58%) Query: 34 QELQKQMGIGSLTDLMSIVQELLDKNLIKLVKQNDELKFQG 74 QEL+ + I ++TD +S++QEL D LI + Q L Q Sbjct: 405 QELEYRFRIRNITDELSLIQELDDTRLIIDLSQQPNLYTQI 445 >3I5J-B nrPDB Length = 327 Score = 28.5 bits (62), Expect = 4.0, Method: Composition-based stats. Identities = 22/95 (23%), Positives = 42/95 (44%), Gaps = 9/95 (9%) Query: 215 YSTTQEILEFITAAQVANVELTPSNIRSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQG 274 YS + + + + ++ P++ S C +L D L +TH YR S+ + G Sbjct: 122 YSPLRYLFHCLQMSSAYVQQMAPASTISNCCILQTADSLRWLTHTAYRTHELSLTYPDAG 181 Query: 275 EG-------EPEAGNKALED--EEEFSIFNYFKMF 300 G E E G + L + E++ + F++ + F Sbjct: 182 LGEHERELWEKEPGWQGLRELMEKQLTAFDWGEAF 216 >5YTI-A nrPDB Length = 411 Score = 28.5 bits (62), Expect = 4.1, Method: Composition-based stats. Identities = 16/69 (23%), Positives = 33/69 (47%), Gaps = 4/69 (5%) Query: 4 MIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTD----LMSIVQELLDKN 59 + +N Q +D+A L ++S + +L +E+Q Q G SL++ +++ + L Sbjct: 61 LFQNNRQAADSALRLEEGILSNAVNSLHRLREIQIQAGNPSLSEEDRKTLAVEAQALLNQ 120 Query: 60 LIKLVKQND 68 L+ D Sbjct: 121 LLDYANTKD 129 >2XD3-A nrPDB Length = 416 Score = 28.1 bits (61), Expect = 5.1, Method: Composition-based stats. Identities = 23/109 (21%), Positives = 41/109 (37%), Gaps = 7/109 (6%) Query: 131 QRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLTIVWRFISENT 190 +++ K ++ + I + + I GPW D + + TI T Sbjct: 234 EKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKAQAFKDAKVNYGVATI-------PT 286 Query: 191 FPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSN 239 PNG + G K VKN +Q+ ++F+ A + V +N Sbjct: 287 LPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQKVLYDKTN 335 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.133 0.369 Lambda K H 0.267 0.0411 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,108,218 Number of Sequences: 36641 Number of extensions: 479464 Number of successful extensions: 1258 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 1237 Number of HSP's gapped (non-prelim): 39 length of query: 317 length of database: 10,336,785 effective HSP length: 97 effective length of query: 220 effective length of database: 6,782,608 effective search space: 1492173760 effective search space used: 1492173760 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 59 (27.3 bits)