BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P32799 (129 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6HU9-k nrPDB 248 4e-67 2Y69-G nrPDB 58 1e-09 2DYR-G nrPDB 57 2e-09 5Z62-G nrPDB 56 4e-09 3RY7-A nrPDB 30 0.35 1NX9-A nrPDB 27 3.1 5UR2-A nrPDB 26 3.7 5FM1-C nrPDB 26 4.5 2D3Y-A nrPDB 26 4.7 3C4V-A nrPDB 25 6.9 >6HU9-k nrPDB Length = 131 Score = 248 bits (634), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 120/120 (100%), Positives = 120/120 (100%) Query: 10 ASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVN 69 ASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVN Sbjct: 1 ASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVN 60 Query: 70 TYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPVVNRHIEHDD 129 TYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPVVNRHIEHDD Sbjct: 61 TYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPVVNRHIEHDD 120 >2Y69-G nrPDB Length = 97 Score = 57.8 bits (138), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 30/84 (35%), Positives = 44/84 (52%), Gaps = 11/84 (13%) Query: 39 ATEKHAKDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEF 98 A H + W ++ +ALP++AL +N++ H+ HRE +P Y Sbjct: 16 AKGDHGGTGARTWRFLTFGLALPSVALCTLNSWL----HSGHRERPAFIP-------YHH 64 Query: 99 MNIRSKPFFWGDGDKTLFWNPVVN 122 + IR+KPF WGDG+ T F NP VN Sbjct: 65 LRIRTKPFSWGDGNHTFFHNPRVN 88 >2DYR-G nrPDB Length = 85 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 30/84 (35%), Positives = 44/84 (52%), Gaps = 11/84 (13%) Query: 39 ATEKHAKDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEF 98 A H + W ++ +ALP++AL +N++ H+ HRE +P Y Sbjct: 4 AKGDHGGTGARTWRFLTFGLALPSVALCTLNSWL----HSGHRERPAFIP-------YHH 52 Query: 99 MNIRSKPFFWGDGDKTLFWNPVVN 122 + IR+KPF WGDG+ T F NP VN Sbjct: 53 LRIRTKPFSWGDGNHTFFHNPRVN 76 >5Z62-G nrPDB Length = 75 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 31/78 (39%), Positives = 45/78 (57%), Gaps = 13/78 (16%) Query: 47 TSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPR--DYEFMNIRSK 104 ++ MW ++ +VALP +A++ +N Y ++ H EH E P Y + IR+K Sbjct: 1 SARMWKTLTFFVALPGVAVSMLNVY-LKSHHGEH----------ERPEFIAYPHLRIRTK 49 Query: 105 PFFWGDGDKTLFWNPVVN 122 PF WGDG+ TLF NP VN Sbjct: 50 PFPWGDGNHTLFHNPHVN 67 >3RY7-A nrPDB Length = 304 Score = 29.6 bits (65), Expect = 0.35, Method: Compositional matrix adjust. Identities = 17/61 (27%), Positives = 28/61 (45%), Gaps = 2/61 (3%) Query: 25 PDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHL 84 P++ A+ + E+ KD +N ++ I + L I L TYF K ++H E Sbjct: 185 PNETEAELLSGIKVTNEQSMKDNANYFLSIGIKTVL--ITLGKQGTYFATKNQSQHIEAY 242 Query: 85 K 85 K Sbjct: 243 K 243 >1NX9-A nrPDB Length = 652 Score = 26.6 bits (57), Expect = 3.1, Method: Compositional matrix adjust. Identities = 16/45 (35%), Positives = 21/45 (46%) Query: 26 DKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVNT 70 DK+ AQ+ M E+ D +MW I W AL + A NT Sbjct: 278 DKILAQRKPTVPMLWEQGLWDQEDMWGAIHAWQALKDADVKAPNT 322 >5UR2-A nrPDB Length = 984 Score = 26.2 bits (56), Expect = 3.7, Method: Compositional matrix adjust. Identities = 22/93 (23%), Positives = 38/93 (40%), Gaps = 20/93 (21%) Query: 40 TEKHAKDTSNMWVKISVWVA--------LPAI------ALTAVNTYFVEKEHAEHREHLK 85 +EK A+D SN ++++ W+A +P I AL VN + Sbjct: 151 SEKEAQDYSNKYMELVTWLAKDAEKWDEVPQIDRDHEGALPKVNV------SVKMTALYS 204 Query: 86 HVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWN 118 + D+ W + + R +P F +K +F N Sbjct: 205 QIKDAAWDESKKILKDRLRPVFRLGMEKGVFVN 237 >5FM1-C nrPDB Length = 473 Score = 26.2 bits (56), Expect = 4.5, Method: Composition-based stats. Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 5/33 (15%) Query: 75 KEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFF 107 KEHA + L +PDS RD + +KPFF Sbjct: 27 KEHAIGTDGLSQLPDSSTERDDD-----TKPFF 54 >2D3Y-A nrPDB Length = 219 Score = 25.8 bits (55), Expect = 4.7, Method: Compositional matrix adjust. Identities = 16/54 (29%), Positives = 26/54 (48%) Query: 35 ESLMATEKHAKDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVP 88 E L A + + + ++ V+VAL IAL A+ +F ++ A H H P Sbjct: 125 EELRACARWTEVELGLLPEVRVYVALGRIALEALLAHFGLRKSAHPFRHGAHYP 178 >3C4V-A nrPDB Length = 426 Score = 25.4 bits (54), Expect = 6.9, Method: Composition-based stats. Identities = 22/75 (29%), Positives = 32/75 (42%), Gaps = 6/75 (8%) Query: 8 RYASSLPPNALKPAFGPPDKVAAQKFKES--LMATEKHAKDTSNMWVKISVWVALPAIAL 65 R+ PP+ L + D VA F ES L+A E A T + ++ LP IA+ Sbjct: 289 RFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAARVG---GLP-IAV 344 Query: 66 TAVNTYFVEKEHAEH 80 T + H+ H Sbjct: 345 AEGETGLLVDGHSPH 359 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6HU9-k nrPDB 196 2e-51 2Y69-G nrPDB 114 8e-27 2DYR-G nrPDB 112 4e-26 5Z62-G nrPDB 96 3e-21 Sequences not found previously or not previously below threshold: 2NLK-A nrPDB 29 0.85 2PBN-A nrPDB 28 0.90 5UM2-A nrPDB 26 5.1 4DOJ-A nrPDB 26 5.1 6HIW-CH nrPDB 26 6.8 1GNX-A nrPDB 25 8.5 CONVERGED! >6HU9-k nrPDB Length = 131 Score = 196 bits (499), Expect = 2e-51, Method: Composition-based stats. Identities = 120/120 (100%), Positives = 120/120 (100%) Query: 10 ASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVN 69 ASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVN Sbjct: 1 ASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVN 60 Query: 70 TYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPVVNRHIEHDD 129 TYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPVVNRHIEHDD Sbjct: 61 TYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPVVNRHIEHDD 120 >2Y69-G nrPDB Length = 97 Score = 114 bits (286), Expect = 8e-27, Method: Composition-based stats. Identities = 30/84 (35%), Positives = 44/84 (52%), Gaps = 11/84 (13%) Query: 39 ATEKHAKDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEF 98 A H + W ++ +ALP++AL +N++ H+ HRE +P Y Sbjct: 16 AKGDHGGTGARTWRFLTFGLALPSVALCTLNSWL----HSGHRERPAFIP-------YHH 64 Query: 99 MNIRSKPFFWGDGDKTLFWNPVVN 122 + IR+KPF WGDG+ T F NP VN Sbjct: 65 LRIRTKPFSWGDGNHTFFHNPRVN 88 >2DYR-G nrPDB Length = 85 Score = 112 bits (280), Expect = 4e-26, Method: Composition-based stats. Identities = 30/84 (35%), Positives = 44/84 (52%), Gaps = 11/84 (13%) Query: 39 ATEKHAKDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEF 98 A H + W ++ +ALP++AL +N++ H+ HRE +P Y Sbjct: 4 AKGDHGGTGARTWRFLTFGLALPSVALCTLNSWL----HSGHRERPAFIP-------YHH 52 Query: 99 MNIRSKPFFWGDGDKTLFWNPVVN 122 + IR+KPF WGDG+ T F NP VN Sbjct: 53 LRIRTKPFSWGDGNHTFFHNPRVN 76 >5Z62-G nrPDB Length = 75 Score = 96.4 bits (238), Expect = 3e-21, Method: Composition-based stats. Identities = 30/76 (39%), Positives = 46/76 (60%), Gaps = 9/76 (11%) Query: 47 TSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPF 106 ++ MW ++ +VALP +A++ +N Y ++ H EH E + + Y + IR+KPF Sbjct: 1 SARMWKTLTFFVALPGVAVSMLNVY-LKSHHGEH-ERPEFI-------AYPHLRIRTKPF 51 Query: 107 FWGDGDKTLFWNPVVN 122 WGDG+ TLF NP VN Sbjct: 52 PWGDGNHTLFHNPHVN 67 >2NLK-A nrPDB Length = 627 Score = 28.6 bits (62), Expect = 0.85, Method: Composition-based stats. Identities = 10/46 (21%), Positives = 19/46 (41%), Gaps = 1/46 (2%) Query: 73 VEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWN 118 + EH+ H E+ KH Y+ ++ +P D + + N Sbjct: 39 ITAEHSNHPEN-KHKNRYINILAYDHSRVKLRPLPGKDSKHSDYIN 83 >2PBN-A nrPDB Length = 313 Score = 28.2 bits (61), Expect = 0.90, Method: Composition-based stats. Identities = 10/46 (21%), Positives = 19/46 (41%), Gaps = 1/46 (2%) Query: 73 VEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWN 118 + EH+ H E+ KH Y+ ++ +P D + + N Sbjct: 47 ITAEHSNHPEN-KHKNRYINILAYDHSRVKLRPLPGKDSKHSDYIN 91 >5UM2-A nrPDB Length = 334 Score = 25.9 bits (55), Expect = 5.1, Method: Composition-based stats. Identities = 19/73 (26%), Positives = 30/73 (41%), Gaps = 1/73 (1%) Query: 35 ESLMATEKHAKDTSNMWVKISVWVALPAIALTAVNT-YFVEKEHAEHREHLKHVPDSEWP 93 E+L+A E+ KD + V +A P++AL N +E AE + P+ + Sbjct: 217 EALLAREELGKDKFEIVVPKLSILAEPSVALVDKNVDKHGTREVAEAYLRYLYAPEGQKL 276 Query: 94 RDYEFMNIRSKPF 106 F R F Sbjct: 277 AAKHFYRPRHPEF 289 >4DOJ-A nrPDB Length = 566 Score = 25.9 bits (55), Expect = 5.1, Method: Composition-based stats. Identities = 10/27 (37%), Positives = 15/27 (55%) Query: 42 KHAKDTSNMWVKISVWVALPAIALTAV 68 +H + +N WV + VA AI LT + Sbjct: 452 QHGQLEANKWVTAAWGVATAAIGLTLL 478 >6HIW-CH nrPDB Length = 282 Score = 25.5 bits (54), Expect = 6.8, Method: Composition-based stats. Identities = 15/57 (26%), Positives = 23/57 (40%), Gaps = 1/57 (1%) Query: 73 VEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPVVNRHIEHDD 129 V A + L +P E RD I ++ FF GD L++ ++ DD Sbjct: 77 VSSHEAVPQTPLVDLPGVERRRDLPADPI-TRLFFQHKGDHALYYGTYDKPSVQDDD 132 >1GNX-A nrPDB Length = 479 Score = 25.1 bits (53), Expect = 8.5, Method: Composition-based stats. Identities = 10/41 (24%), Positives = 17/41 (41%) Query: 15 PNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKIS 55 P L+ A G P++ A++F E D W ++ Sbjct: 137 PQELENAGGWPERATAERFAEYAAIAADALGDRVKTWTTLN 177 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.126 0.389 Lambda K H 0.267 0.0385 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,700,449 Number of Sequences: 36641 Number of extensions: 197907 Number of successful extensions: 494 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 465 Number of HSP's gapped (non-prelim): 30 length of query: 129 length of database: 10,336,785 effective HSP length: 86 effective length of query: 43 effective length of database: 7,185,659 effective search space: 308983337 effective search space used: 308983337 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.1 bits)