BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P32774 (122 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6GYK-V nrPDB 251 5e-68 5M4S-A nrPDB 89 3e-19 5IYA-O nrPDB 89 5e-19 6MZM-X nrPDB 86 3e-18 2W0A-A nrPDB 31 0.10 5US7-A nrPDB 28 1.1 5US9-A nrPDB 28 1.2 2STD-A nrPDB 26 4.8 5URF-A nrPDB 25 6.7 6DGE-A nrPDB 25 7.1 >6GYK-V nrPDB Length = 129 Score = 251 bits (641), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 122/122 (100%), Positives = 122/122 (100%) Query: 1 MAVPGYYELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKL 60 MAVPGYYELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKL Sbjct: 1 MAVPGYYELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKL 60 Query: 61 TVKGNLDTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKK 120 TVKGNLDTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKK Sbjct: 61 TVKGNLDTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKK 120 Query: 121 SE 122 SE Sbjct: 121 SE 122 >5M4S-A nrPDB Length = 209 Score = 89.4 bits (220), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 44/115 (38%), Positives = 73/115 (63%), Gaps = 14/115 (12%) Query: 7 YELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNL 66 Y+LYR +T+GNSL ++LD LI +I LA++VL FDK + L ++++ +G+L Sbjct: 2 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 61 Query: 67 DTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKKS 121 +TY FCD+VWTF++ + +V R+ ++ +I VDK++IVAC+ K + Sbjct: 62 NTYRFCDNVWTFVLNDVEV------REVTE--------LIKVDKVKIVACDGKNT 102 >5IYA-O nrPDB Length = 109 Score = 88.6 bits (218), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 43/115 (37%), Positives = 72/115 (62%), Gaps = 14/115 (12%) Query: 7 YELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNL 66 Y+LYR +T+GNSL ++LD LI +I LA++VL FDK + L ++++ +G+L Sbjct: 3 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 62 Query: 67 DTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKKS 121 +TY FCD+VWTF++ + + R+ ++ +I VDK++IVAC+ K + Sbjct: 63 NTYRFCDNVWTFVLNDVE------FREVTE--------LIKVDKVKIVACDGKNT 103 >6MZM-X nrPDB Length = 97 Score = 86.3 bits (212), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 42/111 (37%), Positives = 70/111 (63%), Gaps = 14/111 (12%) Query: 7 YELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNL 66 Y+LYR +T+GNSL ++LD LI +I LA++VL FDK + L ++++ +G+L Sbjct: 1 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 60 Query: 67 DTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACN 117 +TY FCD+VWTF++ + + R+ ++ +I VDK++IVAC+ Sbjct: 61 NTYRFCDNVWTFVLNDVE------FREVTE--------LIKVDKVKIVACD 97 >2W0A-A nrPDB Length = 455 Score = 31.2 bits (69), Expect = 0.10, Method: Composition-based stats. Identities = 16/46 (34%), Positives = 25/46 (54%) Query: 8 ELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLK 53 EL R +++D LD L DGR + A+R + + V+ ETL+ Sbjct: 271 ELMRHRDAYAAVIDELDELYGDGRSVSFHALRQIPQLENVLKETLR 316 >5US7-A nrPDB Length = 539 Score = 27.7 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 12/27 (44%), Positives = 14/27 (51%), Gaps = 4/27 (14%) Query: 52 LKDNTQSKLTVKGNLDTYGFCDDVWTF 78 L+D T +GN TY C DVW F Sbjct: 378 LRDQT----LYRGNQTTYNLCSDVWMF 400 >5US9-A nrPDB Length = 541 Score = 27.7 bits (60), Expect = 1.2, Method: Composition-based stats. Identities = 9/16 (56%), Positives = 10/16 (62%) Query: 63 KGNLDTYGFCDDVWTF 78 +GN TY C DVW F Sbjct: 387 RGNQTTYNLCSDVWMF 402 >2STD-A nrPDB Length = 172 Score = 25.8 bits (55), Expect = 4.8, Method: Compositional matrix adjust. Identities = 14/38 (36%), Positives = 19/38 (50%), Gaps = 5/38 (13%) Query: 46 KVVAETLKDNTQSKLTVKG-----NLDTYGFCDDVWTF 78 +V + KD T ++T+KG NL Y D VW F Sbjct: 107 RVPHQRYKDTTMKEVTMKGHAHSANLHWYKKIDGVWKF 144 >5URF-A nrPDB Length = 542 Score = 25.4 bits (54), Expect = 6.7, Method: Composition-based stats. Identities = 9/22 (40%), Positives = 12/22 (54%) Query: 57 QSKLTVKGNLDTYGFCDDVWTF 78 + +L KGN TY D+W F Sbjct: 382 RDQLLYKGNQTTYNLVGDIWMF 403 >6DGE-A nrPDB Length = 308 Score = 25.0 bits (53), Expect = 7.1, Method: Compositional matrix adjust. Identities = 15/56 (26%), Positives = 25/56 (44%) Query: 17 NSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNLDTYGFC 72 N+ +D D L + L+ R + +++A+T KD S + V L FC Sbjct: 146 NATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFC 201 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6GYK-V nrPDB 205 3e-54 5M4S-A nrPDB 154 1e-38 5IYA-O nrPDB 149 3e-37 6MZM-X nrPDB 143 2e-35 Sequences not found previously or not previously below threshold: 5US9-A nrPDB 31 0.12 5US7-A nrPDB 30 0.25 2W0A-A nrPDB 29 0.53 3HLD-A nrPDB 27 2.6 5URF-A nrPDB 26 3.8 3LJ5-A nrPDB 26 4.2 3WFL-A nrPDB 26 4.4 5V7X-A nrPDB 26 4.9 3TO3-A nrPDB 25 5.2 3J5V-a nrPDB 25 6.6 2XF8-A nrPDB 25 7.6 CONVERGED! >6GYK-V nrPDB Length = 129 Score = 205 bits (522), Expect = 3e-54, Method: Composition-based stats. Identities = 122/122 (100%), Positives = 122/122 (100%) Query: 1 MAVPGYYELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKL 60 MAVPGYYELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKL Sbjct: 1 MAVPGYYELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKL 60 Query: 61 TVKGNLDTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKK 120 TVKGNLDTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKK Sbjct: 61 TVKGNLDTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKK 120 Query: 121 SE 122 SE Sbjct: 121 SE 122 >5M4S-A nrPDB Length = 209 Score = 154 bits (388), Expect = 1e-38, Method: Composition-based stats. Identities = 44/115 (38%), Positives = 73/115 (63%), Gaps = 14/115 (12%) Query: 7 YELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNL 66 Y+LYR +T+GNSL ++LD LI +I LA++VL FDK + L ++++ +G+L Sbjct: 2 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 61 Query: 67 DTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKKS 121 +TY FCD+VWTF++ + +V R+ ++ +I VDK++IVAC+ K + Sbjct: 62 NTYRFCDNVWTFVLNDVEV------REVTE--------LIKVDKVKIVACDGKNT 102 >5IYA-O nrPDB Length = 109 Score = 149 bits (375), Expect = 3e-37, Method: Composition-based stats. Identities = 43/115 (37%), Positives = 72/115 (62%), Gaps = 14/115 (12%) Query: 7 YELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNL 66 Y+LYR +T+GNSL ++LD LI +I LA++VL FDK + L ++++ +G+L Sbjct: 3 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 62 Query: 67 DTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKKS 121 +TY FCD+VWTF++ + + R+ ++ +I VDK++IVAC+ K + Sbjct: 63 NTYRFCDNVWTFVLNDVE------FREVTE--------LIKVDKVKIVACDGKNT 103 >6MZM-X nrPDB Length = 97 Score = 143 bits (361), Expect = 2e-35, Method: Composition-based stats. Identities = 42/111 (37%), Positives = 70/111 (63%), Gaps = 14/111 (12%) Query: 7 YELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNL 66 Y+LYR +T+GNSL ++LD LI +I LA++VL FDK + L ++++ +G+L Sbjct: 1 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 60 Query: 67 DTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACN 117 +TY FCD+VWTF++ + + R+ ++ +I VDK++IVAC+ Sbjct: 61 NTYRFCDNVWTFVLNDVE------FREVTE--------LIKVDKVKIVACD 97 >5US9-A nrPDB Length = 541 Score = 31.2 bits (69), Expect = 0.12, Method: Composition-based stats. Identities = 9/22 (40%), Positives = 12/22 (54%) Query: 57 QSKLTVKGNLDTYGFCDDVWTF 78 + + +GN TY C DVW F Sbjct: 381 RDQALFRGNQTTYNLCSDVWMF 402 >5US7-A nrPDB Length = 539 Score = 30.1 bits (66), Expect = 0.25, Method: Composition-based stats. Identities = 9/22 (40%), Positives = 12/22 (54%) Query: 57 QSKLTVKGNLDTYGFCDDVWTF 78 + + +GN TY C DVW F Sbjct: 379 RDQTLYRGNQTTYNLCSDVWMF 400 >2W0A-A nrPDB Length = 455 Score = 28.9 bits (63), Expect = 0.53, Method: Composition-based stats. Identities = 16/46 (34%), Positives = 25/46 (54%) Query: 8 ELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLK 53 EL R +++D LD L DGR + A+R + + V+ ETL+ Sbjct: 271 ELMRHRDAYAAVIDELDELYGDGRSVSFHALRQIPQLENVLKETLR 316 >3HLD-A nrPDB Length = 432 Score = 26.6 bits (57), Expect = 2.6, Method: Composition-based stats. Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 4/42 (9%) Query: 23 LDTLISDGRIEASL-AMRVLETFDKVVAETLKDNTQSKLTVK 63 LD + R+ L AM VLE FD L++ + K+T++ Sbjct: 116 LDETVD--RLLPDLSAMPVLEGFDDAGNARLRER-RGKITLR 154 >5URF-A nrPDB Length = 542 Score = 26.2 bits (56), Expect = 3.8, Method: Composition-based stats. Identities = 9/22 (40%), Positives = 12/22 (54%) Query: 57 QSKLTVKGNLDTYGFCDDVWTF 78 + +L KGN TY D+W F Sbjct: 382 RDQLLYKGNQTTYNLVGDIWMF 403 >3LJ5-A nrPDB Length = 725 Score = 25.8 bits (55), Expect = 4.2, Method: Composition-based stats. Identities = 8/31 (25%), Positives = 22/31 (70%) Query: 55 NTQSKLTVKGNLDTYGFCDDVWTFIVKNCQV 85 +T ++L ++ +L+TY F D++ T + ++ ++ Sbjct: 433 DTVNQLNMRADLETYVFQDNLATAMRRDGEI 463 >3WFL-A nrPDB Length = 342 Score = 25.8 bits (55), Expect = 4.4, Method: Composition-based stats. Identities = 12/46 (26%), Positives = 18/46 (39%) Query: 25 TLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNLDTYG 70 L+ DG + LE D VV+ + N + + N YG Sbjct: 72 QLLQDGTATINTGSDGLERLDYVVSSAEQHNVKLIINFVNNWSDYG 117 >5V7X-A nrPDB Length = 752 Score = 25.8 bits (55), Expect = 4.9, Method: Composition-based stats. Identities = 17/71 (23%), Positives = 33/71 (46%), Gaps = 9/71 (12%) Query: 48 VAETLKDNTQSKLTVKGNLDTYG---FCDDVW-TFIVKNC-----QVTVEDSHRDASQNG 98 + E++K T+ + V G LD YG F D+ + FI+ C Q+ +E + ++ + Sbjct: 368 INESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTLKEEQEEY 427 Query: 99 SGDSQSVISVD 109 + +D Sbjct: 428 IREDIEWTHID 438 >3TO3-A nrPDB Length = 619 Score = 25.5 bits (54), Expect = 5.2, Method: Composition-based stats. Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 2/45 (4%) Query: 7 YELY--RRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVA 49 YE Y R T G +D+L + DG+ E + LE +VV Sbjct: 68 YECYGRERITFGRISIDSLIVRVQDGKQEIQSVAQFLEEVFRVVN 112 >3J5V-a nrPDB Length = 320 Score = 25.5 bits (54), Expect = 6.6, Method: Composition-based stats. Identities = 22/88 (25%), Positives = 37/88 (42%), Gaps = 2/88 (2%) Query: 28 SDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNLDTYGFCDDVWTFIVKNCQVTV 87 + GR S+ + E KVV L + +L V + F D I + C++ + Sbjct: 161 TQGRSYESINDEIAEKIRKVV--LLVNQNHGRLDVHDVANWVRFTDKHNYLIPQVCELHI 218 Query: 88 EDSHRDASQNGSGDSQSVISVDKLRIVA 115 E + +DA SQ + +D + VA Sbjct: 219 ETTRKDAENVPEAISQLSLYLDPSKEVA 246 >2XF8-A nrPDB Length = 338 Score = 25.1 bits (53), Expect = 7.6, Method: Composition-based stats. Identities = 10/39 (25%), Positives = 19/39 (48%) Query: 40 VLETFDKVVAETLKDNTQSKLTVKGNLDTYGFCDDVWTF 78 V F+ + D TQ++++ + T +CD+ W F Sbjct: 283 VSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGF 321 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.129 0.341 Lambda K H 0.267 0.0397 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,041,139 Number of Sequences: 36641 Number of extensions: 126399 Number of successful extensions: 366 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 26 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 334 Number of HSP's gapped (non-prelim): 35 length of query: 122 length of database: 10,336,785 effective HSP length: 85 effective length of query: 37 effective length of database: 7,222,300 effective search space: 267225100 effective search space used: 267225100 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 52 (24.7 bits)