BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P32585 (307 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4GWQ-E nrPDB 630 0.0 3RJ1-E nrPDB 548 e-156 1JPD-X nrPDB 29 3.0 >4GWQ-E nrPDB Length = 307 Score = 630 bits (1626), Expect = 0.0, Method: Compositional matrix adjust. Identities = 307/307 (100%), Positives = 307/307 (100%) Query: 1 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE 60 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE Sbjct: 1 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE 60 Query: 61 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN 120 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN Sbjct: 61 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN 120 Query: 121 STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV 180 STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV Sbjct: 121 STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV 180 Query: 181 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE 240 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE Sbjct: 181 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE 240 Query: 241 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV 300 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV Sbjct: 241 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV 300 Query: 301 AHGNILI 307 AHGNILI Sbjct: 301 AHGNILI 307 >3RJ1-E nrPDB Length = 275 Score = 548 bits (1411), Expect = e-156, Method: Compositional matrix adjust. Identities = 275/307 (89%), Positives = 275/307 (89%), Gaps = 32/307 (10%) Query: 1 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE 60 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE Sbjct: 1 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE 60 Query: 61 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN 120 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNI Sbjct: 61 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNI------------ 108 Query: 121 STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV 180 NSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV Sbjct: 109 --------------------NSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV 148 Query: 181 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE 240 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE Sbjct: 149 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE 208 Query: 241 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV 300 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV Sbjct: 209 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV 268 Query: 301 AHGNILI 307 AHGNILI Sbjct: 269 AHGNILI 275 >1JPD-X nrPDB Length = 324 Score = 28.9 bits (63), Expect = 3.0, Method: Compositional matrix adjust. Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 2/74 (2%) Query: 3 QQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVEPN 62 QQ SL IG + +I+ T + G P + NS T+W+ + V N L+ Sbjct: 106 QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAG--AKLLKVKLDNHLISER 163 Query: 63 RIKLSKEVPFSYLI 76 + + VP + LI Sbjct: 164 MVAIRTAVPDATLI 177 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4GWQ-E nrPDB 585 e-168 3RJ1-E nrPDB 531 e-151 Sequences not found previously or not previously below threshold: 1JPD-X nrPDB 30 0.88 CONVERGED! >4GWQ-E nrPDB Length = 307 Score = 585 bits (1509), Expect = e-168, Method: Composition-based stats. Identities = 307/307 (100%), Positives = 307/307 (100%) Query: 1 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE 60 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE Sbjct: 1 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE 60 Query: 61 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN 120 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN Sbjct: 61 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN 120 Query: 121 STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV 180 STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV Sbjct: 121 STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV 180 Query: 181 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE 240 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE Sbjct: 181 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE 240 Query: 241 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV 300 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV Sbjct: 241 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV 300 Query: 301 AHGNILI 307 AHGNILI Sbjct: 301 AHGNILI 307 >3RJ1-E nrPDB Length = 275 Score = 531 bits (1367), Expect = e-151, Method: Composition-based stats. Identities = 275/307 (89%), Positives = 275/307 (89%), Gaps = 32/307 (10%) Query: 1 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE 60 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE Sbjct: 1 MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE 60 Query: 61 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN 120 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNI Sbjct: 61 PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNI------------ 108 Query: 121 STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV 180 NSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV Sbjct: 109 --------------------NSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV 148 Query: 181 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE 240 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE Sbjct: 149 SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE 208 Query: 241 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV 300 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV Sbjct: 209 AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV 268 Query: 301 AHGNILI 307 AHGNILI Sbjct: 269 AHGNILI 275 >1JPD-X nrPDB Length = 324 Score = 30.4 bits (67), Expect = 0.88, Method: Composition-based stats. Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 2/74 (2%) Query: 3 QQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVEPN 62 QQ SL IG + +I+ T + G P + NS T+W+ + V N L+ Sbjct: 106 QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAK--LLKVKLDNHLISER 163 Query: 63 RIKLSKEVPFSYLI 76 + + VP + LI Sbjct: 164 MVAIRTAVPDATLI 177 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.132 0.389 Lambda K H 0.267 0.0383 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,340,493 Number of Sequences: 36641 Number of extensions: 433219 Number of successful extensions: 770 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 759 Number of HSP's gapped (non-prelim): 22 length of query: 307 length of database: 10,336,785 effective HSP length: 97 effective length of query: 210 effective length of database: 6,782,608 effective search space: 1424347680 effective search space used: 1424347680 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 59 (27.4 bits)