BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P28519 (371 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5LCL-B nrPDB 775 0.0 5G34-A nrPDB 255 2e-68 1D4U-A nrPDB 55 7e-08 2ENK-A nrPDB 32 0.38 1XHS-A nrPDB 29 2.6 5FP2-A nrPDB 29 4.1 3KGX-A nrPDB 28 4.9 >5LCL-B nrPDB Length = 371 Score = 775 bits (2001), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/371 (100%), Positives = 371/371 (100%) Query: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA Sbjct: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60 Query: 61 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY 120 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY Sbjct: 61 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY 120 Query: 121 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP 180 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP Sbjct: 121 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP 180 Query: 181 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD 240 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD Sbjct: 181 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD 240 Query: 241 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH 300 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH Sbjct: 241 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH 300 Query: 301 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT 360 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT Sbjct: 301 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT 360 Query: 361 DCGLETEEIDI 371 DCGLETEEIDI Sbjct: 361 DCGLETEEIDI 371 >5G34-A nrPDB Length = 131 Score = 255 bits (652), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 119/119 (100%), Positives = 119/119 (100%) Query: 188 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED 247 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED Sbjct: 2 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED 61 Query: 248 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY 306 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY Sbjct: 62 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY 120 >1D4U-A nrPDB Length = 111 Score = 54.7 bits (130), Expect = 7e-08, Method: Compositional matrix adjust. Identities = 34/100 (34%), Positives = 54/100 (54%), Gaps = 3/100 (3%) Query: 191 CIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELND-EDLF 249 C EC MD L D F L C C ++ +K+ L+TKTE K++Y L D +L E Sbjct: 8 CEECGKEF-MDSYLMDHFDLPTCDDC-RDADDKHKLITKTEAKQEYLLKDCDLEKREPPL 65 Query: 250 HRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDE 289 + K NPH + M+L+++ ++ + + WG +E L+E Sbjct: 66 KFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEE 105 >2ENK-A nrPDB Length = 101 Score = 32.3 bits (72), Expect = 0.38, Method: Compositional matrix adjust. Identities = 20/73 (27%), Positives = 41/73 (56%), Gaps = 3/73 (4%) Query: 244 NDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRRE 303 +D + ++ + +PH T + +++R +VEA + + WG E L E + R+E + RE Sbjct: 29 SDLEQLRKIRRRSPHEDTES-FTVYLRSDVEAKSLEVWGSPEALAREKKLRKEAEIEYRE 87 Query: 304 KKY--EKKIKEMR 314 + + +K ++E R Sbjct: 88 RLFRNQKILREYR 100 >1XHS-A nrPDB Length = 121 Score = 29.3 bits (64), Expect = 2.6, Method: Composition-based stats. Identities = 12/31 (38%), Positives = 19/31 (61%) Query: 315 LKTRAQEYTNRLREKKHGKAHIHHFSDPVDG 345 L+TR EY +L + +G A ++ + PVDG Sbjct: 69 LRTRGGEYARQLIQTPYGSAWMYVYQRPVDG 99 >5FP2-A nrPDB Length = 725 Score = 28.9 bits (63), Expect = 4.1, Method: Compositional matrix adjust. Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 3/28 (10%) Query: 85 YGSGVVDGSKRDASVLDKRPTDRIRPSI 112 YGSG G +++ KRPTDR+R S+ Sbjct: 145 YGSGAAGGV---VNIITKRPTDRLRGSM 169 >3KGX-A nrPDB Length = 393 Score = 28.5 bits (62), Expect = 4.9, Method: Compositional matrix adjust. Identities = 26/109 (23%), Positives = 43/109 (39%), Gaps = 10/109 (9%) Query: 269 VRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKYEKKIK------EMRLKTRAQEY 322 + C E+ A E+GL+ W+R E AH + E +K E+RL T Sbjct: 270 LYCLRESLALI---AEQGLENCWRRHREATAHLHKHLQEMGLKFFVKDPEIRLPTITTVT 326 Query: 323 TNRLREKKHGKAHIH-HFSDPVDGGIDEDGYQIQRRRCTDCGLETEEID 370 + +++ HFS + GG+ ++ R TE +D Sbjct: 327 VPAGYNWRDIVSYVLDHFSIEISGGLGPTEERVLRIGLLGYNATTENVD 375 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5LCL-B nrPDB 712 0.0 5G34-A nrPDB 220 9e-58 1D4U-A nrPDB 159 3e-39 Sequences not found previously or not previously below threshold: 2ENK-A nrPDB 49 3e-06 4QMK-A nrPDB 31 0.96 1LU1-A nrPDB 30 1.2 5B16-A nrPDB 30 1.2 1XHS-A nrPDB 30 1.7 6C95-A nrPDB 30 2.4 3JB9-c nrPDB 29 2.5 1G7Y-A nrPDB 29 2.7 6G18-t nrPDB 29 3.1 >5LCL-B nrPDB Length = 371 Score = 712 bits (1838), Expect = 0.0, Method: Composition-based stats. Identities = 371/371 (100%), Positives = 371/371 (100%) Query: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA Sbjct: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60 Query: 61 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY 120 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY Sbjct: 61 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY 120 Query: 121 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP 180 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP Sbjct: 121 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP 180 Query: 181 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD 240 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD Sbjct: 181 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD 240 Query: 241 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH 300 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH Sbjct: 241 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH 300 Query: 301 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT 360 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT Sbjct: 301 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT 360 Query: 361 DCGLETEEIDI 371 DCGLETEEIDI Sbjct: 361 DCGLETEEIDI 371 >5G34-A nrPDB Length = 131 Score = 220 bits (560), Expect = 9e-58, Method: Composition-based stats. Identities = 119/119 (100%), Positives = 119/119 (100%) Query: 188 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED 247 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED Sbjct: 2 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED 61 Query: 248 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY 306 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY Sbjct: 62 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY 120 >1D4U-A nrPDB Length = 111 Score = 159 bits (401), Expect = 3e-39, Method: Composition-based stats. Identities = 35/106 (33%), Positives = 57/106 (53%), Gaps = 3/106 (2%) Query: 191 CIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELND-EDLF 249 C EC MD L D F L C C ++ +K+ L+TKTE K++Y L D +L E Sbjct: 8 CEECGKEF-MDSYLMDHFDLPTCDDC-RDADDKHKLITKTEAKQEYLLKDCDLEKREPPL 65 Query: 250 HRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRRE 295 + K NPH + M+L+++ ++ + + WG +E L+E + R+ Sbjct: 66 KFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQ 111 >2ENK-A nrPDB Length = 101 Score = 49.3 bits (116), Expect = 3e-06, Method: Composition-based stats. Identities = 22/94 (23%), Positives = 45/94 (47%), Gaps = 6/94 (6%) Query: 223 KYALLTKTECKEDYFLTDPELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWG 282 + +T ++ L +L ++ + +PH T +++R +VEA + + WG Sbjct: 11 QNNFITGVRAINEFCLKSSDLEQ---LRKIRRRSPHEDT-ESFTVYLRSDVEAKSLEVWG 66 Query: 283 GEEGLDEEWQRREEGKAHRREKKY--EKKIKEMR 314 E L E + R+E + RE+ + +K ++E R Sbjct: 67 SPEALAREKKLRKEAEIEYRERLFRNQKILREYR 100 >4QMK-A nrPDB Length = 677 Score = 30.8 bits (68), Expect = 0.96, Method: Composition-based stats. Identities = 31/125 (24%), Positives = 46/125 (36%), Gaps = 30/125 (24%) Query: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60 MTPEQK L+A + + G L +L R L +A+ DD A Sbjct: 406 MTPEQKQHLQAQARQTVS-----GHLQQRELERERHEFPSLNDAVMAM------DDQMLA 454 Query: 61 AVHVPNHNGQP-------------------SALANTNTNTTSLYGSGVVDGSKRDASVLD 101 +V V N +A+A N +TSL + + + R+ L Sbjct: 455 SVQVDLQNDAAGAEALRFRKDAQQALQALDTAIAEANQTSTSLVITPKLASALRNLDALA 514 Query: 102 KRPTD 106 +RP D Sbjct: 515 RRPED 519 >1LU1-A nrPDB Length = 253 Score = 30.4 bits (67), Expect = 1.2, Method: Composition-based stats. Identities = 23/87 (26%), Positives = 39/87 (44%), Gaps = 7/87 (8%) Query: 68 NGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEYDFATMQN 127 NG+ + + T TSL + +V S+R + +L ++R+ + +Y+ F+ Sbjct: 158 NGENAEILITYNAATSLLVASLVHPSRRTSYIL----SERVDITNELPEYVSVGFSATTG 213 Query: 128 LNGGYINPKDKLP---NSDFTDDQEFE 151 L+ GYI D L S DD E Sbjct: 214 LSEGYIETHDVLSWSFASKLPDDSTAE 240 >5B16-A nrPDB Length = 986 Score = 30.4 bits (67), Expect = 1.2, Method: Composition-based stats. Identities = 24/108 (22%), Positives = 46/108 (42%), Gaps = 16/108 (14%) Query: 226 LLTKTECKEDYFLTDPELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEA----FAFKKW 281 L +TE KE P++ L+ L+ P + ++ + E + ++ Sbjct: 883 LTLRTEGKE------PDI---PLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQA 933 Query: 282 GGEEGLDEEWQRREEGKAHRR---EKKYEKKIKEMRLKTRAQEYTNRL 326 +D + AH++ E+KY +++KEMR + QE +RL Sbjct: 934 EMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQERESRL 981 >1XHS-A nrPDB Length = 121 Score = 30.0 bits (66), Expect = 1.7, Method: Composition-based stats. Identities = 12/31 (38%), Positives = 19/31 (61%) Query: 315 LKTRAQEYTNRLREKKHGKAHIHHFSDPVDG 345 L+TR EY +L + +G A ++ + PVDG Sbjct: 69 LRTRGGEYARQLIQTPYGSAWMYVYQRPVDG 99 >6C95-A nrPDB Length = 866 Score = 29.7 bits (65), Expect = 2.4, Method: Composition-based stats. Identities = 19/82 (23%), Positives = 35/82 (42%), Gaps = 6/82 (7%) Query: 202 PVLHD----VFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDEDLFHRLEKPNP 257 P LH+ +F VC+ SK+ + + K E + T+P+ +E R P Sbjct: 706 PWLHECMIRLFNTAVCE--SKDLSDTVRTVLKQEMNRLFGATNPKNFNETFLKRNSDSLP 763 Query: 258 HSGTFARMQLFVRCEVEAFAFK 279 H + A+M ++ + A + Sbjct: 764 HRLSAAKMVYYLDPSSQKRAIE 785 >3JB9-c nrPDB Length = 639 Score = 29.3 bits (64), Expect = 2.5, Method: Composition-based stats. Identities = 20/81 (24%), Positives = 38/81 (46%), Gaps = 3/81 (3%) Query: 264 RMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKYEKKIK--EMRLKTRAQE 321 M R VE A +++G + D + R+E + +R +K +K++ E+ K A E Sbjct: 185 DMAQDQRRPVEEVALERYGSLKEFDFALEERDELELRKRTRKVQKEVPTGELYEKRCADE 244 Query: 322 YTNRLREKKHGKAHIHHFSDP 342 + R ++ +G + DP Sbjct: 245 FKTR-KDSGNGATYYKVIPDP 264 >1G7Y-A nrPDB Length = 253 Score = 29.3 bits (64), Expect = 2.7, Method: Composition-based stats. Identities = 22/93 (23%), Positives = 41/93 (44%), Gaps = 12/93 (12%) Query: 68 NGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEYDFATMQN 127 NGQ + + T TSL + +V S+R + ++ ++R+ + +Y+ F+ Sbjct: 158 NGQNAEILITYNAATSLLVASLVHPSRRTSYIV----SERVDITNELPEYVSIGFSATTG 213 Query: 128 LNGGYINPKD--------KLPNSDFTDDQEFES 152 L+ GY D KLP+ T+ + S Sbjct: 214 LSEGYTETHDVLSWSFASKLPDDSTTEPLDIAS 246 >6G18-t nrPDB Length = 475 Score = 29.3 bits (64), Expect = 3.1, Method: Composition-based stats. Identities = 16/76 (21%), Positives = 36/76 (47%), Gaps = 2/76 (2%) Query: 248 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKYE 307 + L K + R+Q+ ++ + + E +++ R++ K R+E++ E Sbjct: 385 PLNVLPKKGLTAKQTERIQMINGSDLPKVSTQPRSKNESKEDKRARKQAIKEERKERRVE 444 Query: 308 KKIKEM--RLKTRAQE 321 KK ++ +L+ R QE Sbjct: 445 KKANKLAFKLEKRRQE 460 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.133 0.381 Lambda K H 0.267 0.0412 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,261,849 Number of Sequences: 36641 Number of extensions: 716798 Number of successful extensions: 1456 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 24 Number of HSP's that attempted gapping in prelim test: 1423 Number of HSP's gapped (non-prelim): 48 length of query: 371 length of database: 10,336,785 effective HSP length: 99 effective length of query: 272 effective length of database: 6,709,326 effective search space: 1824936672 effective search space used: 1824936672 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 60 (27.7 bits)