BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= P28519
(371 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
5LCL-B nrPDB 775 0.0
5G34-A nrPDB 255 2e-68
1D4U-A nrPDB 55 7e-08
2ENK-A nrPDB 32 0.38
1XHS-A nrPDB 29 2.6
5FP2-A nrPDB 29 4.1
3KGX-A nrPDB 28 4.9
>5LCL-B nrPDB
Length = 371
Score = 775 bits (2001), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 371/371 (100%), Positives = 371/371 (100%)
Query: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60
MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA
Sbjct: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60
Query: 61 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY 120
AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY
Sbjct: 61 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY 120
Query: 121 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP 180
DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP
Sbjct: 121 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP 180
Query: 181 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD 240
PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD
Sbjct: 181 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD 240
Query: 241 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH 300
PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH
Sbjct: 241 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH 300
Query: 301 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT 360
RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT
Sbjct: 301 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT 360
Query: 361 DCGLETEEIDI 371
DCGLETEEIDI
Sbjct: 361 DCGLETEEIDI 371
>5G34-A nrPDB
Length = 131
Score = 255 bits (652), Expect = 2e-68, Method: Compositional matrix adjust.
Identities = 119/119 (100%), Positives = 119/119 (100%)
Query: 188 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED 247
APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED
Sbjct: 2 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED 61
Query: 248 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY 306
LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY
Sbjct: 62 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY 120
>1D4U-A nrPDB
Length = 111
Score = 54.7 bits (130), Expect = 7e-08, Method: Compositional matrix adjust.
Identities = 34/100 (34%), Positives = 54/100 (54%), Gaps = 3/100 (3%)
Query: 191 CIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELND-EDLF 249
C EC MD L D F L C C ++ +K+ L+TKTE K++Y L D +L E
Sbjct: 8 CEECGKEF-MDSYLMDHFDLPTCDDC-RDADDKHKLITKTEAKQEYLLKDCDLEKREPPL 65
Query: 250 HRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDE 289
+ K NPH + M+L+++ ++ + + WG +E L+E
Sbjct: 66 KFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEE 105
>2ENK-A nrPDB
Length = 101
Score = 32.3 bits (72), Expect = 0.38, Method: Compositional matrix adjust.
Identities = 20/73 (27%), Positives = 41/73 (56%), Gaps = 3/73 (4%)
Query: 244 NDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRRE 303
+D + ++ + +PH T + +++R +VEA + + WG E L E + R+E + RE
Sbjct: 29 SDLEQLRKIRRRSPHEDTES-FTVYLRSDVEAKSLEVWGSPEALAREKKLRKEAEIEYRE 87
Query: 304 KKY--EKKIKEMR 314
+ + +K ++E R
Sbjct: 88 RLFRNQKILREYR 100
>1XHS-A nrPDB
Length = 121
Score = 29.3 bits (64), Expect = 2.6, Method: Composition-based stats.
Identities = 12/31 (38%), Positives = 19/31 (61%)
Query: 315 LKTRAQEYTNRLREKKHGKAHIHHFSDPVDG 345
L+TR EY +L + +G A ++ + PVDG
Sbjct: 69 LRTRGGEYARQLIQTPYGSAWMYVYQRPVDG 99
>5FP2-A nrPDB
Length = 725
Score = 28.9 bits (63), Expect = 4.1, Method: Compositional matrix adjust.
Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 3/28 (10%)
Query: 85 YGSGVVDGSKRDASVLDKRPTDRIRPSI 112
YGSG G +++ KRPTDR+R S+
Sbjct: 145 YGSGAAGGV---VNIITKRPTDRLRGSM 169
>3KGX-A nrPDB
Length = 393
Score = 28.5 bits (62), Expect = 4.9, Method: Compositional matrix adjust.
Identities = 26/109 (23%), Positives = 43/109 (39%), Gaps = 10/109 (9%)
Query: 269 VRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKYEKKIK------EMRLKTRAQEY 322
+ C E+ A E+GL+ W+R E AH + E +K E+RL T
Sbjct: 270 LYCLRESLALI---AEQGLENCWRRHREATAHLHKHLQEMGLKFFVKDPEIRLPTITTVT 326
Query: 323 TNRLREKKHGKAHIH-HFSDPVDGGIDEDGYQIQRRRCTDCGLETEEID 370
+ +++ HFS + GG+ ++ R TE +D
Sbjct: 327 VPAGYNWRDIVSYVLDHFSIEISGGLGPTEERVLRIGLLGYNATTENVD 375
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
5LCL-B nrPDB 712 0.0
5G34-A nrPDB 220 9e-58
1D4U-A nrPDB 159 3e-39
Sequences not found previously or not previously below threshold:
2ENK-A nrPDB 49 3e-06
4QMK-A nrPDB 31 0.96
1LU1-A nrPDB 30 1.2
5B16-A nrPDB 30 1.2
1XHS-A nrPDB 30 1.7
6C95-A nrPDB 30 2.4
3JB9-c nrPDB 29 2.5
1G7Y-A nrPDB 29 2.7
6G18-t nrPDB 29 3.1
>5LCL-B nrPDB
Length = 371
Score = 712 bits (1838), Expect = 0.0, Method: Composition-based stats.
Identities = 371/371 (100%), Positives = 371/371 (100%)
Query: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60
MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA
Sbjct: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60
Query: 61 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY 120
AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY
Sbjct: 61 AVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEY 120
Query: 121 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP 180
DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP
Sbjct: 121 DFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKKEQLERKMLYENAP 180
Query: 181 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD 240
PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD
Sbjct: 181 PPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTD 240
Query: 241 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH 300
PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH
Sbjct: 241 PELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH 300
Query: 301 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT 360
RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT
Sbjct: 301 RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDEDGYQIQRRRCT 360
Query: 361 DCGLETEEIDI 371
DCGLETEEIDI
Sbjct: 361 DCGLETEEIDI 371
>5G34-A nrPDB
Length = 131
Score = 220 bits (560), Expect = 9e-58, Method: Composition-based stats.
Identities = 119/119 (100%), Positives = 119/119 (100%)
Query: 188 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED 247
APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED
Sbjct: 2 APKCIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDED 61
Query: 248 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY 306
LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY
Sbjct: 62 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKY 120
>1D4U-A nrPDB
Length = 111
Score = 159 bits (401), Expect = 3e-39, Method: Composition-based stats.
Identities = 35/106 (33%), Positives = 57/106 (53%), Gaps = 3/106 (2%)
Query: 191 CIECHINIEMDPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELND-EDLF 249
C EC MD L D F L C C ++ +K+ L+TKTE K++Y L D +L E
Sbjct: 8 CEECGKEF-MDSYLMDHFDLPTCDDC-RDADDKHKLITKTEAKQEYLLKDCDLEKREPPL 65
Query: 250 HRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRRE 295
+ K NPH + M+L+++ ++ + + WG +E L+E + R+
Sbjct: 66 KFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQ 111
>2ENK-A nrPDB
Length = 101
Score = 49.3 bits (116), Expect = 3e-06, Method: Composition-based stats.
Identities = 22/94 (23%), Positives = 45/94 (47%), Gaps = 6/94 (6%)
Query: 223 KYALLTKTECKEDYFLTDPELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWG 282
+ +T ++ L +L ++ + +PH T +++R +VEA + + WG
Sbjct: 11 QNNFITGVRAINEFCLKSSDLEQ---LRKIRRRSPHEDT-ESFTVYLRSDVEAKSLEVWG 66
Query: 283 GEEGLDEEWQRREEGKAHRREKKY--EKKIKEMR 314
E L E + R+E + RE+ + +K ++E R
Sbjct: 67 SPEALAREKKLRKEAEIEYRERLFRNQKILREYR 100
>4QMK-A nrPDB
Length = 677
Score = 30.8 bits (68), Expect = 0.96, Method: Composition-based stats.
Identities = 31/125 (24%), Positives = 46/125 (36%), Gaps = 30/125 (24%)
Query: 1 MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTSGSNRDDNAAA 60
MTPEQK L+A + + G L +L R L +A+ DD A
Sbjct: 406 MTPEQKQHLQAQARQTVS-----GHLQQRELERERHEFPSLNDAVMAM------DDQMLA 454
Query: 61 AVHVPNHNGQP-------------------SALANTNTNTTSLYGSGVVDGSKRDASVLD 101
+V V N +A+A N +TSL + + + R+ L
Sbjct: 455 SVQVDLQNDAAGAEALRFRKDAQQALQALDTAIAEANQTSTSLVITPKLASALRNLDALA 514
Query: 102 KRPTD 106
+RP D
Sbjct: 515 RRPED 519
>1LU1-A nrPDB
Length = 253
Score = 30.4 bits (67), Expect = 1.2, Method: Composition-based stats.
Identities = 23/87 (26%), Positives = 39/87 (44%), Gaps = 7/87 (8%)
Query: 68 NGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEYDFATMQN 127
NG+ + + T TSL + +V S+R + +L ++R+ + +Y+ F+
Sbjct: 158 NGENAEILITYNAATSLLVASLVHPSRRTSYIL----SERVDITNELPEYVSVGFSATTG 213
Query: 128 LNGGYINPKDKLP---NSDFTDDQEFE 151
L+ GYI D L S DD E
Sbjct: 214 LSEGYIETHDVLSWSFASKLPDDSTAE 240
>5B16-A nrPDB
Length = 986
Score = 30.4 bits (67), Expect = 1.2, Method: Composition-based stats.
Identities = 24/108 (22%), Positives = 46/108 (42%), Gaps = 16/108 (14%)
Query: 226 LLTKTECKEDYFLTDPELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEA----FAFKKW 281
L +TE KE P++ L+ L+ P + ++ + E + ++
Sbjct: 883 LTLRTEGKE------PDI---PLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQA 933
Query: 282 GGEEGLDEEWQRREEGKAHRR---EKKYEKKIKEMRLKTRAQEYTNRL 326
+D + AH++ E+KY +++KEMR + QE +RL
Sbjct: 934 EMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQERESRL 981
>1XHS-A nrPDB
Length = 121
Score = 30.0 bits (66), Expect = 1.7, Method: Composition-based stats.
Identities = 12/31 (38%), Positives = 19/31 (61%)
Query: 315 LKTRAQEYTNRLREKKHGKAHIHHFSDPVDG 345
L+TR EY +L + +G A ++ + PVDG
Sbjct: 69 LRTRGGEYARQLIQTPYGSAWMYVYQRPVDG 99
>6C95-A nrPDB
Length = 866
Score = 29.7 bits (65), Expect = 2.4, Method: Composition-based stats.
Identities = 19/82 (23%), Positives = 35/82 (42%), Gaps = 6/82 (7%)
Query: 202 PVLHD----VFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDEDLFHRLEKPNP 257
P LH+ +F VC+ SK+ + + K E + T+P+ +E R P
Sbjct: 706 PWLHECMIRLFNTAVCE--SKDLSDTVRTVLKQEMNRLFGATNPKNFNETFLKRNSDSLP 763
Query: 258 HSGTFARMQLFVRCEVEAFAFK 279
H + A+M ++ + A +
Sbjct: 764 HRLSAAKMVYYLDPSSQKRAIE 785
>3JB9-c nrPDB
Length = 639
Score = 29.3 bits (64), Expect = 2.5, Method: Composition-based stats.
Identities = 20/81 (24%), Positives = 38/81 (46%), Gaps = 3/81 (3%)
Query: 264 RMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKYEKKIK--EMRLKTRAQE 321
M R VE A +++G + D + R+E + +R +K +K++ E+ K A E
Sbjct: 185 DMAQDQRRPVEEVALERYGSLKEFDFALEERDELELRKRTRKVQKEVPTGELYEKRCADE 244
Query: 322 YTNRLREKKHGKAHIHHFSDP 342
+ R ++ +G + DP
Sbjct: 245 FKTR-KDSGNGATYYKVIPDP 264
>1G7Y-A nrPDB
Length = 253
Score = 29.3 bits (64), Expect = 2.7, Method: Composition-based stats.
Identities = 22/93 (23%), Positives = 41/93 (44%), Gaps = 12/93 (12%)
Query: 68 NGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEYDFATMQN 127
NGQ + + T TSL + +V S+R + ++ ++R+ + +Y+ F+
Sbjct: 158 NGQNAEILITYNAATSLLVASLVHPSRRTSYIV----SERVDITNELPEYVSIGFSATTG 213
Query: 128 LNGGYINPKD--------KLPNSDFTDDQEFES 152
L+ GY D KLP+ T+ + S
Sbjct: 214 LSEGYTETHDVLSWSFASKLPDDSTTEPLDIAS 246
>6G18-t nrPDB
Length = 475
Score = 29.3 bits (64), Expect = 3.1, Method: Composition-based stats.
Identities = 16/76 (21%), Positives = 36/76 (47%), Gaps = 2/76 (2%)
Query: 248 LFHRLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAHRREKKYE 307
+ L K + R+Q+ ++ + + E +++ R++ K R+E++ E
Sbjct: 385 PLNVLPKKGLTAKQTERIQMINGSDLPKVSTQPRSKNESKEDKRARKQAIKEERKERRVE 444
Query: 308 KKIKEM--RLKTRAQE 321
KK ++ +L+ R QE
Sbjct: 445 KKANKLAFKLEKRRQE 460
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.313 0.133 0.381
Lambda K H
0.267 0.0412 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,261,849
Number of Sequences: 36641
Number of extensions: 716798
Number of successful extensions: 1456
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 24
Number of HSP's that attempted gapping in prelim test: 1423
Number of HSP's gapped (non-prelim): 48
length of query: 371
length of database: 10,336,785
effective HSP length: 99
effective length of query: 272
effective length of database: 6,709,326
effective search space: 1824936672
effective search space used: 1824936672
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 60 (27.7 bits)