BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P28026 (358 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4F0A-B nrPDB 655 0.0 6AHY-B nrPDB 224 6e-59 4KRR-A nrPDB 80 1e-15 5OFK-A nrPDB 32 0.45 5F8B-A nrPDB 30 1.9 >4F0A-B nrPDB Length = 316 Score = 655 bits (1689), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/316 (100%), Positives = 316/316 (100%) Query: 23 AWSVNNFLMTGPKAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNGLRSA 82 AWSVNNFLMTGPKAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNGLRSA Sbjct: 1 AWSVNNFLMTGPKAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNGLRSA 60 Query: 83 TRETSFVHAISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGER 142 TRETSFVHAISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGER Sbjct: 61 TRETSFVHAISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGER 120 Query: 143 ISKLFVDGLETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEF 202 ISKLFVDGLETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEF Sbjct: 121 ISKLFVDGLETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEF 180 Query: 203 RDIGNHLKIKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLK 262 RDIGNHLKIKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLK Sbjct: 181 RDIGNHLKIKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLK 240 Query: 263 NISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCT 322 NISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCT Sbjct: 241 NISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCT 300 Query: 323 VKCEQCKQVVIKHFCA 338 VKCEQCKQVVIKHFCA Sbjct: 301 VKCEQCKQVVIKHFCA 316 >6AHY-B nrPDB Length = 319 Score = 224 bits (570), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 123/302 (40%), Positives = 169/302 (55%), Gaps = 10/302 (3%) Query: 37 YLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNG-LRSATRETSFVHAISSA 95 Y+ SVA G + GI+EC++QF RWNC LA L ATRE++FVHAI+SA Sbjct: 26 YIEIMPSVAEGVKLGIQECQHQFRGRRWNCTTIDDSLAIFGPVLDKATRESAFVHAIASA 85 Query: 96 GVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGERISKLFVDGLETGQ 155 GV + +TR+C+ G CGCD G G GW WGGCS++A+FG +S+ F D E Sbjct: 86 GVAFAVTRSCAEGTSTICGCDSHHKGPPG-EGWKWGGCSEDADFGVLVSREFADARENRP 144 Query: 156 DARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEFRDIGNHLKIKHDQ 215 DAR+ MN HNNEAGR + + M CKCHG+SGSC ++TCW +FR IG+ LK K+D Sbjct: 145 DARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDS 204 Query: 216 ALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLKNISLGLQGTEGRE 275 A ++ ++K + G R A F +L++ E+SP++C N G GT R Sbjct: 205 ASEMVVEKHRESRGWVETLR-AKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRT 263 Query: 276 CLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCTVKCEQCKQVVIKH 335 C N++ C LC CG + + C+C FHWCC V C++C ++ H Sbjct: 264 C-----NVTSHGIDGCDLLC--CGRGHNTRTEKRKEKCHCIFHWCCYVSCQECIRIYDVH 316 Query: 336 FC 337 C Sbjct: 317 TC 318 >4KRR-A nrPDB Length = 221 Score = 80.5 bits (197), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 61/241 (25%), Positives = 111/241 (46%), Gaps = 33/241 (13%) Query: 35 KAYLTYSASVAVGAQNGIEECKYQFAWERWNCP-ESTLQLATHNGLRSATRETSFVHAIS 93 +A L++ G + ++ C+ F W+RWNCP + +Q + S RE +V AIS Sbjct: 3 QAPLSWEDITGKGLKQALDSCQQSFQWQRWNCPSQDFVQKNSKPEENSPNREDVYVAAIS 62 Query: 94 SAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGERISKLFVDGLET 153 A +++TLT++C+ G CGC ++ + + E+ K F Sbjct: 63 MAAIVHTLTKDCANGVIAGCGCTENALNVPCAH--------EPTKALEQYEKHF------ 108 Query: 154 GQDARALMNLHNNEAGRLAVKETMKRTCKCH---GISGSCSIQTCWLQLAEFRDIGNHLK 210 G + A+ HN ++ ++++ C+C + G C + C L F I L Sbjct: 109 GSGSGAIG--HNRRVVGALLQRSLEQECRCKQPGAVQGECQEEECVAVLKPFEAIAQDLL 166 Query: 211 IKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLKNISLGLQG 270 +D A++LE G S++ + + ++ L+F++DSP+YC ++ + GL G Sbjct: 167 QMYDDAIQLE--------GASSN----LKIMWQNIPLDSLVFMQDSPNYCERDAT-GLWG 213 Query: 271 T 271 T Sbjct: 214 T 214 >5OFK-A nrPDB Length = 339 Score = 32.0 bits (71), Expect = 0.45, Method: Compositional matrix adjust. Identities = 21/77 (27%), Positives = 31/77 (40%), Gaps = 9/77 (11%) Query: 141 ERISKLFVDGLETGQDARALMNLHNNEAGRL------AVKETMKRTCKCHGISGSCSIQT 194 E I K F+ E +A+ N +N E + VK + HGI C I Sbjct: 165 EYIEKAFIWAHEADPNAKLFYNDYNTEISKKRDFIYNMVKNLKSKGIPIHGIGMQCHINV 224 Query: 195 CWLQLAEFRDIGNHLKI 211 W ++E I N +K+ Sbjct: 225 NWPSVSE---IENSIKL 238 >5F8B-A nrPDB Length = 244 Score = 29.6 bits (65), Expect = 1.9, Method: Compositional matrix adjust. Identities = 13/35 (37%), Positives = 18/35 (51%) Query: 259 YCLKNISLGLQGTEGRECLQSGKNLSQWERRSCKR 293 +C NISLG Q G+ Q N+S+W C + Sbjct: 159 FCAGNISLGFQFFYGKAWRQQNPNVSRWYEMVCHQ 193 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4F0A-B nrPDB 481 e-136 6AHY-B nrPDB 431 e-121 4KRR-A nrPDB 268 3e-72 Sequences not found previously or not previously below threshold: 3MC2-A nrPDB 29 3.3 5MLC-9 nrPDB 29 4.7 3ZD9-A nrPDB 28 5.3 1S8G-A nrPDB 28 6.8 1UMV-X nrPDB 28 7.5 CONVERGED! >4F0A-B nrPDB Length = 316 Score = 481 bits (1238), Expect = e-136, Method: Composition-based stats. Identities = 316/316 (100%), Positives = 316/316 (100%) Query: 23 AWSVNNFLMTGPKAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNGLRSA 82 AWSVNNFLMTGPKAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNGLRSA Sbjct: 1 AWSVNNFLMTGPKAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNGLRSA 60 Query: 83 TRETSFVHAISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGER 142 TRETSFVHAISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGER Sbjct: 61 TRETSFVHAISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGER 120 Query: 143 ISKLFVDGLETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEF 202 ISKLFVDGLETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEF Sbjct: 121 ISKLFVDGLETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEF 180 Query: 203 RDIGNHLKIKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLK 262 RDIGNHLKIKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLK Sbjct: 181 RDIGNHLKIKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLK 240 Query: 263 NISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCT 322 NISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCT Sbjct: 241 NISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCT 300 Query: 323 VKCEQCKQVVIKHFCA 338 VKCEQCKQVVIKHFCA Sbjct: 301 VKCEQCKQVVIKHFCA 316 >6AHY-B nrPDB Length = 319 Score = 431 bits (1108), Expect = e-121, Method: Composition-based stats. Identities = 123/304 (40%), Positives = 170/304 (55%), Gaps = 10/304 (3%) Query: 35 KAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNG-LRSATRETSFVHAIS 93 + Y+ SVA G + GI+EC++QF RWNC LA L ATRE++FVHAI+ Sbjct: 24 RNYIEIMPSVAEGVKLGIQECQHQFRGRRWNCTTIDDSLAIFGPVLDKATRESAFVHAIA 83 Query: 94 SAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGERISKLFVDGLET 153 SAGV + +TR+C+ G CGCD G G GW WGGCS++A+FG +S+ F D E Sbjct: 84 SAGVAFAVTRSCAEGTSTICGCDSHHKGPPG-EGWKWGGCSEDADFGVLVSREFADAREN 142 Query: 154 GQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEFRDIGNHLKIKH 213 DAR+ MN HNNEAGR + + M CKCHG+SGSC ++TCW +FR IG+ LK K+ Sbjct: 143 RPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKY 202 Query: 214 DQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLKNISLGLQGTEG 273 D A ++ ++K + G R A F +L++ E+SP++C N G GT Sbjct: 203 DSASEMVVEKHRESRGWVETLR-AKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRD 261 Query: 274 RECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCTVKCEQCKQVVI 333 R C N++ C LC CG + + C+C FHWCC V C++C ++ Sbjct: 262 RTC-----NVTSHGIDGCDLLC--CGRGHNTRTEKRKEKCHCIFHWCCYVSCQECIRIYD 314 Query: 334 KHFC 337 H C Sbjct: 315 VHTC 318 >4KRR-A nrPDB Length = 221 Score = 268 bits (684), Expect = 3e-72, Method: Composition-based stats. Identities = 61/241 (25%), Positives = 111/241 (46%), Gaps = 33/241 (13%) Query: 35 KAYLTYSASVAVGAQNGIEECKYQFAWERWNCP-ESTLQLATHNGLRSATRETSFVHAIS 93 +A L++ G + ++ C+ F W+RWNCP + +Q + S RE +V AIS Sbjct: 3 QAPLSWEDITGKGLKQALDSCQQSFQWQRWNCPSQDFVQKNSKPEENSPNREDVYVAAIS 62 Query: 94 SAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGERISKLFVDGLET 153 A +++TLT++C+ G CGC ++ + + E+ K F Sbjct: 63 MAAIVHTLTKDCANGVIAGCGCTENALNVPCAH--------EPTKALEQYEKHF------ 108 Query: 154 GQDARALMNLHNNEAGRLAVKETMKRTCKCH---GISGSCSIQTCWLQLAEFRDIGNHLK 210 G + A+ HN ++ ++++ C+C + G C + C L F I L Sbjct: 109 GSGSGAIG--HNRRVVGALLQRSLEQECRCKQPGAVQGECQEEECVAVLKPFEAIAQDLL 166 Query: 211 IKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLKNISLGLQG 270 +D A++LE G S++ + + ++ L+F++DSP+YC ++ + GL G Sbjct: 167 QMYDDAIQLE--------GASSN----LKIMWQNIPLDSLVFMQDSPNYCERDAT-GLWG 213 Query: 271 T 271 T Sbjct: 214 T 214 >3MC2-A nrPDB Length = 687 Score = 29.0 bits (63), Expect = 3.3, Method: Composition-based stats. Identities = 34/221 (15%), Positives = 65/221 (29%), Gaps = 26/221 (11%) Query: 37 YLTYSASVAVGAQN----GIEECKYQFAWER-WNCPESTLQLATHNGLRSATRETSFVHA 91 Y+ A G Q G + C W W P STL + +AT +S Sbjct: 102 YVVAMAKKGTGFQLNQLRGKKSCHTGLGWSAGWYVPLSTLLPSGSRETAAATFFSSSCVP 161 Query: 92 ISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGERISKLFVDGL 151 + + +L + C+ D C C W C + K Sbjct: 162 CADGKMFPSLCQLCAGKGTDKCACSSRE---PYFGSWGALKCLQDGTADVSFVKHLTVFE 218 Query: 152 ETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEFRDIGNHLKI 211 A+ + + L + +R + + C+L + Sbjct: 219 -------AMPTKADRDQYELLCMDNTRRPVE--------EYEQCYLARVPSHVVVARSVD 263 Query: 212 KHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIF 252 + +++ + + G ++ + F S G +L+F Sbjct: 264 GKEDSIQELLRVAQEHFG---KDKSSPFQLFGSPHGEDLLF 301 >5MLC-9 nrPDB Length = 271 Score = 28.6 bits (62), Expect = 4.7, Method: Composition-based stats. Identities = 14/44 (31%), Positives = 22/44 (50%), Gaps = 1/44 (2%) Query: 258 DYCLKNISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLR 301 + +N + LQG EC + N+S W R S + DCG++ Sbjct: 15 SFRRRNSCVSLQGVSDMECNIARTNVSVW-RSSANYVRMDCGVK 57 >3ZD9-A nrPDB Length = 251 Score = 28.2 bits (61), Expect = 5.3, Method: Composition-based stats. Identities = 18/85 (21%), Positives = 25/85 (29%), Gaps = 11/85 (12%) Query: 168 AGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEFRDIGNHLKIKHDQALKLEM--DKRK 225 A L + + + + H + GS ++TC L I H Q D Sbjct: 2 AVHLLIVDALNLIRRIHAVQGSPCVETCQHAL--------DQLIMHSQPTHAVAVFDDEN 53 Query: 226 MRSGNSADNRGAIADAFSSVAGSEL 250 SG R A EL Sbjct: 54 RSSGW-RHQRLPDYKAGRPPMPEEL 77 >1S8G-A nrPDB Length = 121 Score = 27.8 bits (60), Expect = 6.8, Method: Composition-based stats. Identities = 13/60 (21%), Positives = 23/60 (38%), Gaps = 7/60 (11%) Query: 278 QSGKN-LSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCTVKCEQCKQVVIKHF 336 ++GKN ++ + C +CG + + C C H CC K C ++ Sbjct: 12 ETGKNAITSYGSYGC-----NCGWGHRGQPKDATDRC-CFVHKCCYKKLTDCNHKTDRYS 65 >1UMV-X nrPDB Length = 122 Score = 27.8 bits (60), Expect = 7.5, Method: Composition-based stats. Identities = 12/46 (26%), Positives = 20/46 (43%), Gaps = 1/46 (2%) Query: 298 CGLRVEEKKTEIISSCNCKFHWCCTVKCEQCKQVVIKHFCARRERD 343 CGL + + T+ C C H CC K C + + +++ D Sbjct: 28 CGLGGQGQPTDATDRC-CFVHDCCYGKVTGCNPKIDSYTYSKKNGD 72 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.123 0.365 Lambda K H 0.267 0.0376 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,583,135 Number of Sequences: 36641 Number of extensions: 452422 Number of successful extensions: 1113 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 26 Number of HSP's that attempted gapping in prelim test: 1088 Number of HSP's gapped (non-prelim): 36 length of query: 358 length of database: 10,336,785 effective HSP length: 98 effective length of query: 260 effective length of database: 6,745,967 effective search space: 1753951420 effective search space used: 1753951420 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 59 (27.5 bits)