BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P28005 (173 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AH3-E nrPDB 351 1e-97 6AHR-E nrPDB 45 2e-05 2CZV-C nrPDB 43 7e-05 2AV5-A nrPDB 42 2e-04 3WZ0-C nrPDB 38 0.003 2OGJ-A nrPDB 28 1.7 6CJ7-A nrPDB 28 1.8 5Z9R-A nrPDB 28 3.0 2X8H-A nrPDB 27 3.5 >6AH3-E nrPDB Length = 173 Score = 351 bits (900), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 173/173 (100%), Positives = 173/173 (100%) Query: 1 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL 60 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL Sbjct: 1 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL 60 Query: 61 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVS 120 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVS Sbjct: 61 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVS 120 Query: 121 GTIKKIEQFAMRRNSKILNIIKCSQSSHLSDNDFIINDFKKIGRENENENEDD 173 GTIKKIEQFAMRRNSKILNIIKCSQSSHLSDNDFIINDFKKIGRENENENEDD Sbjct: 121 GTIKKIEQFAMRRNSKILNIIKCSQSSHLSDNDFIINDFKKIGRENENENEDD 173 >6AHR-E nrPDB Length = 163 Score = 45.1 bits (105), Expect = 2e-05, Method: Compositional matrix adjust. Identities = 37/144 (25%), Positives = 66/144 (45%), Gaps = 22/144 (15%) Query: 1 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL 60 MVR K RY+L E++ D R S D + S+ +R +++ Sbjct: 1 MVRFKHRYLLCELV--SDDPRC--------------RLSLDDRVLSSL---VRDTIARVH 41 Query: 61 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKI---GDVDGLIVNPV 117 G +G+A C+ ++Y + T ++RC +E LV AL ++ + G N + Sbjct: 42 GTFGAAACSIGFAVRYLNAYTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTL 101 Query: 118 KVSGTIKKIEQFAMRRNSKILNII 141 V GTI+ ++F ++ N + L I+ Sbjct: 102 HVGGTIRTCQKFLIQYNRRQLLIL 125 >2CZV-C nrPDB Length = 120 Score = 42.7 bits (99), Expect = 7e-05, Method: Compositional matrix adjust. Identities = 40/127 (31%), Positives = 62/127 (48%), Gaps = 27/127 (21%) Query: 2 VRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNLG 61 +R K+RYI FEII S D + + + I +S LG Sbjct: 12 LRDKNRYIAFEII------------------------SDGDFTKDEVKELIWKSSLEVLG 47 Query: 62 DYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVSG 121 + G+A L +K+ N T TGI+R RE + + ALML+S+ + LI+ + VSG Sbjct: 48 ETGTAIVKPWL-IKFDPN-TKTGIVRSDREYVEYLRFALMLVSEF-NGKRLIIRTLGVSG 104 Query: 122 TIKKIEQ 128 TIK++++ Sbjct: 105 TIKRLKR 111 >2AV5-A nrPDB Length = 120 Score = 41.6 bits (96), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 32/81 (39%), Positives = 44/81 (54%), Gaps = 12/81 (14%) Query: 59 NLGDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDG--LIVNP 116 LG+ G+AK L +K F T TGIIR R VI +L L+S D++G I+ Sbjct: 45 TLGELGTAKAKPWL-IK-FDETTQTGIIRSDRNHVYDVIFSLTLVS---DINGNKAIIKV 99 Query: 117 VKVSGTIKKIE-----QFAMR 132 + VSGTIK+++ QF R Sbjct: 100 LGVSGTIKRLKRKFLSQFGWR 120 >3WZ0-C nrPDB Length = 120 Score = 37.7 bits (86), Expect = 0.003, Method: Compositional matrix adjust. Identities = 39/127 (30%), Positives = 62/127 (48%), Gaps = 27/127 (21%) Query: 2 VRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNLG 61 +R K+RYI F++I E K D IK + E SLS LG Sbjct: 12 LRDKNRYIAFQVI--------GERPFKKD-------------EIKKAVWEA--SLS-ALG 47 Query: 62 DYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVSG 121 GSA+ +K F K+ TGI+R R+ + + AL ++++I + +I + VSG Sbjct: 48 YLGSARAKPWF-IK-FDEKSQTGIVRVDRKHVEELRFALTMLTEI-NGSKVIFRTLGVSG 104 Query: 122 TIKKIEQ 128 TIK++++ Sbjct: 105 TIKRLKR 111 >2OGJ-A nrPDB Length = 417 Score = 28.5 bits (62), Expect = 1.7, Method: Compositional matrix adjust. Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 3/75 (4%) Query: 58 LNLGDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDG-LIVNP 116 LNLG G CN + +L+ + I+ C+ E+ + ++ + S + + G V P Sbjct: 133 LNLGSIGLVACNRVPELRDIKDIDLDRILECYAENSEHIVGLKVRASHV--ITGSWGVTP 190 Query: 117 VKVSGTIKKIEQFAM 131 VK+ I KI + M Sbjct: 191 VKLGKKIAKILKVPM 205 >6CJ7-A nrPDB Length = 390 Score = 28.5 bits (62), Expect = 1.8, Method: Compositional matrix adjust. Identities = 20/76 (26%), Positives = 38/76 (50%), Gaps = 7/76 (9%) Query: 96 VIMALMLMSKIGDV-----DGLIVNPVKVSGTIKKIEQFAMRRNSKILNIIKCSQSSHLS 150 +++A++ G+ L + P K + KK+ + +RNS+ NI+K + + LS Sbjct: 45 ILLAMLQQGAAGNTLDEITRALQMTPEKSAEIFKKVNEEIQKRNSR--NILKTANNVFLS 102 Query: 151 DNDFIINDFKKIGREN 166 +N + FK+I N Sbjct: 103 ENFNLNPQFKRIAVNN 118 >5Z9R-A nrPDB Length = 281 Score = 27.7 bits (60), Expect = 3.0, Method: Compositional matrix adjust. Identities = 14/42 (33%), Positives = 24/42 (57%), Gaps = 4/42 (9%) Query: 6 SRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKS 47 RY + E I P V +S K D++ SHHR + A +++++ Sbjct: 73 GRYQVIEGIISP----VNDSYGKKDLVASHHRVAMARLALQT 110 >2X8H-A nrPDB Length = 598 Score = 27.3 bits (59), Expect = 3.5, Method: Composition-based stats. Identities = 27/112 (24%), Positives = 45/112 (40%), Gaps = 29/112 (25%) Query: 16 PPTDTN---VEESVSKADILLSHHRASPADVSIKSILQEIR---RSLSLNLGDYGSA--K 67 PP D + ++V A ++L P +K +L E + ++ L+ GSA K Sbjct: 2 PPADGTSQWLRKTVDSAAVILFSKTTCPYCKKVKDVLAEAKIKHATIELDQLSNGSAIQK 61 Query: 68 C---------------------NSLLQLKYFSNKTSTGIIRCHREDCDLVIM 98 C +S LKY+SN GI+ + D DL+++ Sbjct: 62 CLASFSKIETVPQMFVRGKFIGDSQTVLKYYSNDELAGIVNESKYDYDLIVI 113 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AH3-E nrPDB 253 3e-68 6AHR-E nrPDB 160 3e-40 2CZV-C nrPDB 116 8e-27 2AV5-A nrPDB 99 7e-22 Sequences not found previously or not previously below threshold: 3WZ0-C nrPDB 87 3e-18 2BGN-E nrPDB 26 7.8 >6AH3-E nrPDB Length = 173 Score = 253 bits (645), Expect = 3e-68, Method: Composition-based stats. Identities = 173/173 (100%), Positives = 173/173 (100%) Query: 1 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL 60 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL Sbjct: 1 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL 60 Query: 61 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVS 120 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVS Sbjct: 61 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVS 120 Query: 121 GTIKKIEQFAMRRNSKILNIIKCSQSSHLSDNDFIINDFKKIGRENENENEDD 173 GTIKKIEQFAMRRNSKILNIIKCSQSSHLSDNDFIINDFKKIGRENENENEDD Sbjct: 121 GTIKKIEQFAMRRNSKILNIIKCSQSSHLSDNDFIINDFKKIGRENENENEDD 173 >6AHR-E nrPDB Length = 163 Score = 160 bits (404), Expect = 3e-40, Method: Composition-based stats. Identities = 37/144 (25%), Positives = 66/144 (45%), Gaps = 22/144 (15%) Query: 1 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL 60 MVR K RY+L E++ D R S D + S+ +R +++ Sbjct: 1 MVRFKHRYLLCELV--SDDPRC--------------RLSLDDRVLSSL---VRDTIARVH 41 Query: 61 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKI---GDVDGLIVNPV 117 G +G+A C+ ++Y + T ++RC +E LV AL ++ + G N + Sbjct: 42 GTFGAAACSIGFAVRYLNAYTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTL 101 Query: 118 KVSGTIKKIEQFAMRRNSKILNII 141 V GTI+ ++F ++ N + L I+ Sbjct: 102 HVGGTIRTCQKFLIQYNRRQLLIL 125 >2CZV-C nrPDB Length = 120 Score = 116 bits (289), Expect = 8e-27, Method: Composition-based stats. Identities = 42/136 (30%), Positives = 65/136 (47%), Gaps = 28/136 (20%) Query: 2 VRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNLG 61 +R K+RYI FEII S D + + + I +S LG Sbjct: 12 LRDKNRYIAFEII------------------------SDGDFTKDEVKELIWKSSLEVLG 47 Query: 62 DYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVSG 121 + G+A L +K+ N T TGI+R RE + + ALML+S+ + LI+ + VSG Sbjct: 48 ETGTAIVKPWL-IKFDPN-TKTGIVRSDREYVEYLRFALMLVSEF-NGKRLIIRTLGVSG 104 Query: 122 TIKKIEQ-FAMRRNSK 136 TIK++++ F + K Sbjct: 105 TIKRLKRKFLAKYGWK 120 >2AV5-A nrPDB Length = 120 Score = 99.5 bits (246), Expect = 7e-22, Method: Composition-based stats. Identities = 40/136 (29%), Positives = 57/136 (41%), Gaps = 32/136 (23%) Query: 2 VRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNLG 61 +R K RYI F++I S + I + I + LG Sbjct: 12 LRDKKRYIAFKVI------------------------SENQFNKDEIKEAIWNACLRTLG 47 Query: 62 DYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVSG 121 + G+AK L F T TGIIR R VI +L L+S I + + I+ + VSG Sbjct: 48 ELGTAKAKPWLI--KFDETTQTGIIRSDRNHVYDVIFSLTLVSDI-NGNKAIIKVLGVSG 104 Query: 122 TIKKIE-----QFAMR 132 TIK+++ QF R Sbjct: 105 TIKRLKRKFLSQFGWR 120 >3WZ0-C nrPDB Length = 120 Score = 87.1 bits (214), Expect = 3e-18, Method: Composition-based stats. Identities = 31/136 (22%), Positives = 56/136 (41%), Gaps = 32/136 (23%) Query: 2 VRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNLG 61 +R K+RYI F++I I + + + LG Sbjct: 12 LRDKNRYIAFQVIGERP------------------------FKKDEIKKAVWEASLSALG 47 Query: 62 DYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKIGDVDGLIVNPVKVSG 121 GSA+ F K+ TGI+R R+ + + AL ++++I + +I + VSG Sbjct: 48 YLGSARAKPWFI--KFDEKSQTGIVRVDRKHVEELRFALTMLTEI-NGSKVIFRTLGVSG 104 Query: 122 TIKKIEQ-----FAMR 132 TIK++++ + R Sbjct: 105 TIKRLKRKFLAEYGWR 120 >2BGN-E nrPDB Length = 363 Score = 26.3 bits (56), Expect = 7.8, Method: Composition-based stats. Identities = 16/96 (16%), Positives = 38/96 (39%), Gaps = 8/96 (8%) Query: 3 RLKSRYILFEII-FPPTDTNVEESVSKADILLSHHRASP-------ADVSIKSILQEIRR 54 R + I +E + D V V + LL++ + P D++ ++ + + Sbjct: 76 RDAIKRIAYEFVEMKAKDGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVSLVNQ 135 Query: 55 SLSLNLGDYGSAKCNSLLQLKYFSNKTSTGIIRCHR 90 L D+G + L +++ + +S + C + Sbjct: 136 GLQEGERDFGVKVRSILCCMRHQPSWSSEVVELCKK 171 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.124 0.312 Lambda K H 0.267 0.0382 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,583,618 Number of Sequences: 36641 Number of extensions: 177517 Number of successful extensions: 473 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 448 Number of HSP's gapped (non-prelim): 19 length of query: 173 length of database: 10,336,785 effective HSP length: 90 effective length of query: 83 effective length of database: 7,039,095 effective search space: 584244885 effective search space used: 584244885 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 55 (25.9 bits)