BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P26441 (200 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1CNT-1 nrPDB 389 e-109 1C9Y-A nrPDB 33 0.075 4ZRX-A nrPDB 29 1.7 5YFP-F nrPDB 28 2.8 3C98-B nrPDB 27 4.5 2K8H-A nrPDB 27 4.6 4AT3-A nrPDB 26 9.5 >1CNT-1 nrPDB Length = 187 Score = 389 bits (1000), Expect = e-109, Method: Compositional matrix adjust. Identities = 187/187 (100%), Positives = 187/187 (100%) Query: 1 MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVAS 60 MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVAS Sbjct: 1 MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVAS 60 Query: 61 TDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFA 120 TDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFA Sbjct: 61 TDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFA 120 Query: 121 YQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFIS 180 YQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFIS Sbjct: 121 YQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFIS 180 Query: 181 SHQTGIP 187 SHQTGIP Sbjct: 181 SHQTGIP 187 >1C9Y-A nrPDB Length = 321 Score = 33.1 bits (74), Expect = 0.075, Method: Compositional matrix adjust. Identities = 20/62 (32%), Positives = 31/62 (50%), Gaps = 3/62 (4%) Query: 29 SDLTALTESYVKHQG---LNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHV 85 + +T L E Y K G L N L++A G V TD W + E ++ LQA++ + V Sbjct: 194 ASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGREEEKKKRLQAFQGYQV 253 Query: 86 LL 87 + Sbjct: 254 TM 255 >4ZRX-A nrPDB Length = 586 Score = 28.9 bits (63), Expect = 1.7, Method: Compositional matrix adjust. Identities = 15/52 (28%), Positives = 26/52 (50%), Gaps = 2/52 (3%) Query: 100 PTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGL 151 P G+F +H L Q AF+ +++ I LE P +A +N+ + G+ Sbjct: 533 PASGEFSNIMHNPLPQTVAFSQKVQARFIKLEATTP--DATVAKVNMNEIGV 582 >5YFP-F nrPDB Length = 910 Score = 28.1 bits (61), Expect = 2.8, Method: Compositional matrix adjust. Identities = 11/26 (42%), Positives = 18/26 (69%) Query: 131 EYKIPRNEADGMPINVGDGGLFEKKL 156 E++I N+ D MP+++ D L+EK L Sbjct: 500 EFQILLNDDDFMPLSINDKTLYEKVL 525 >3C98-B nrPDB Length = 279 Score = 27.3 bits (59), Expect = 4.5, Method: Compositional matrix adjust. Identities = 34/158 (21%), Positives = 66/158 (41%), Gaps = 33/158 (20%) Query: 24 ARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTF 83 A K+RS L ++ +S + +GLN++ SAD L+ +T Sbjct: 98 ANKVRSKLKSIEQSIEQEEGLNRS----SAD----------------------LRIRKTQ 131 Query: 84 HVLLARLLEDQQVHFTPTEGDFHQAIHTLL---LQVAAFAYQIEELMILLEYKIPRNEAD 140 H L+R + + T+ D+ + + L++ EEL +LE P A Sbjct: 132 HSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFAS 191 Query: 141 G--MPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDL 176 G M ++ L E + ++++ + ++R +HD+ Sbjct: 192 GIIMDSSISKQALSEIETRHSEIIK--LENSIRELHDM 227 >2K8H-A nrPDB Length = 110 Score = 27.3 bits (59), Expect = 4.6, Method: Compositional matrix adjust. Identities = 15/42 (35%), Positives = 22/42 (52%) Query: 26 KIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSEL 67 K R+ L L ++Y K QG+++N DG P+ T EL Sbjct: 44 KSRTALKKLIDTYCKKQGISRNSVRFLFDGTPIDETKTPEEL 85 >4AT3-A nrPDB Length = 299 Score = 26.2 bits (56), Expect = 9.5, Method: Compositional matrix adjust. Identities = 13/47 (27%), Positives = 24/47 (51%) Query: 27 IRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERL 73 + D + Y+KH LNK + D + +A + +ELT+++ L Sbjct: 85 VEGDPLIMVFEYMKHGDLNKFLRAHGPDAVLMAEGNPPTELTQSQML 131 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1CNT-1 nrPDB 401 e-113 Sequences not found previously or not previously below threshold: 1C9Y-A nrPDB 35 0.025 2K8H-A nrPDB 28 2.0 4ZRX-A nrPDB 28 2.4 2WVZ-A nrPDB 28 3.5 2ADD-A nrPDB 27 4.3 3C98-B nrPDB 27 5.6 5YFP-F nrPDB 27 5.7 CONVERGED! >1CNT-1 nrPDB Length = 187 Score = 401 bits (1031), Expect = e-113, Method: Composition-based stats. Identities = 187/187 (100%), Positives = 187/187 (100%) Query: 1 MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVAS 60 MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVAS Sbjct: 1 MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVAS 60 Query: 61 TDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFA 120 TDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFA Sbjct: 61 TDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFA 120 Query: 121 YQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFIS 180 YQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFIS Sbjct: 121 YQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFIS 180 Query: 181 SHQTGIP 187 SHQTGIP Sbjct: 181 SHQTGIP 187 >1C9Y-A nrPDB Length = 321 Score = 34.7 bits (78), Expect = 0.025, Method: Composition-based stats. Identities = 20/62 (32%), Positives = 31/62 (50%), Gaps = 3/62 (4%) Query: 29 SDLTALTESYVKHQG---LNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHV 85 + +T L E Y K G L N L++A G V TD W + E ++ LQA++ + V Sbjct: 194 ASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGREEEKKKRLQAFQGYQV 253 Query: 86 LL 87 + Sbjct: 254 TM 255 >2K8H-A nrPDB Length = 110 Score = 28.5 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 15/42 (35%), Positives = 22/42 (52%) Query: 26 KIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSEL 67 K R+ L L ++Y K QG+++N DG P+ T EL Sbjct: 44 KSRTALKKLIDTYCKKQGISRNSVRFLFDGTPIDETKTPEEL 85 >4ZRX-A nrPDB Length = 586 Score = 28.1 bits (61), Expect = 2.4, Method: Composition-based stats. Identities = 12/36 (33%), Positives = 18/36 (50%) Query: 100 PTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIP 135 P G+F +H L Q AF+ +++ I LE P Sbjct: 533 PASGEFSNIMHNPLPQTVAFSQKVQARFIKLEATTP 568 >2WVZ-A nrPDB Length = 744 Score = 27.7 bits (60), Expect = 3.5, Method: Composition-based stats. Identities = 21/85 (24%), Positives = 36/85 (42%), Gaps = 15/85 (17%) Query: 34 LTESYVKHQGLNKNIN---LDSADGMPVASTDQWSE-LTEAERLQENLQAYRTFHVLLAR 89 + S++ + N+N D+ + + D W++ L + E NL YRTF+ L R Sbjct: 230 IASSFISFEQAAANMNELGKDNIEQLAQKGKDAWNQVLGKIEVEGGNLDQYRTFYSCLYR 289 Query: 90 LL-----------EDQQVHFTPTEG 103 L Q +H++P G Sbjct: 290 SLLFPRKFYELDANGQPIHYSPYNG 314 >2ADD-A nrPDB Length = 543 Score = 27.3 bits (59), Expect = 4.3, Method: Composition-based stats. Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 2/46 (4%) Query: 134 IPRNEADGMPINVGDGGL--FEKKLWGLKVLQELSQWTVRSIHDLR 177 +P ++ I G GL F + LW + ++L QW V I +LR Sbjct: 293 VPETDSQADDIEKGWAGLQSFPRALWIDRNGKQLIQWPVEEIEELR 338 >3C98-B nrPDB Length = 279 Score = 27.0 bits (58), Expect = 5.6, Method: Composition-based stats. Identities = 34/158 (21%), Positives = 66/158 (41%), Gaps = 33/158 (20%) Query: 24 ARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTF 83 A K+RS L ++ +S + +GLN++ SAD L+ +T Sbjct: 98 ANKVRSKLKSIEQSIEQEEGLNRS----SAD----------------------LRIRKTQ 131 Query: 84 HVLLARLLEDQQVHFTPTEGDFHQAIHTLL---LQVAAFAYQIEELMILLEYKIPRNEAD 140 H L+R + + T+ D+ + + L++ EEL +LE P A Sbjct: 132 HSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFAS 191 Query: 141 G--MPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDL 176 G M ++ L E + ++++ + ++R +HD+ Sbjct: 192 GIIMDSSISKQALSEIETRHSEIIK--LENSIRELHDM 227 >5YFP-F nrPDB Length = 910 Score = 27.0 bits (58), Expect = 5.7, Method: Composition-based stats. Identities = 11/26 (42%), Positives = 18/26 (69%) Query: 131 EYKIPRNEADGMPINVGDGGLFEKKL 156 E++I N+ D MP+++ D L+EK L Sbjct: 500 EFQILLNDDDFMPLSINDKTLYEKVL 525 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.134 0.398 Lambda K H 0.267 0.0424 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,868,449 Number of Sequences: 36641 Number of extensions: 355295 Number of successful extensions: 911 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 900 Number of HSP's gapped (non-prelim): 17 length of query: 200 length of database: 10,336,785 effective HSP length: 92 effective length of query: 108 effective length of database: 6,965,813 effective search space: 752307804 effective search space used: 752307804 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 57 (26.5 bits)