BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= P25814
(116 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
1A6F-A nrPDB 231 4e-62
1D6T-A nrPDB 117 1e-27
2LJP-A nrPDB 47 2e-06
6CQC-A nrPDB 42 6e-05
1LPA-B nrPDB 28 1.3
4C2X-A nrPDB 27 1.4
5HUF-A nrPDB 27 1.7
1UJ5-A nrPDB 27 1.8
4U0O-B nrPDB 27 2.2
2PVS-A nrPDB 26 3.6
2QDX-A nrPDB 25 5.2
>1A6F-A nrPDB
Length = 119
Score = 231 bits (590), Expect = 4e-62, Method: Compositional matrix adjust.
Identities = 115/116 (99%), Positives = 116/116 (100%)
Query: 1 MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60
+KKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI
Sbjct: 4 LKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 63
Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 116
KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK
Sbjct: 64 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 119
>1D6T-A nrPDB
Length = 117
Score = 117 bits (294), Expect = 1e-27, Method: Compositional matrix adjust.
Identities = 55/112 (49%), Positives = 81/112 (72%)
Query: 1 MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60
++K R+KKN DFQ+++K G SVANRQFV+YT + E D R+G+SVSKK+GNAV+RN+I
Sbjct: 3 LEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFRLGISVSKKLGNAVLRNKI 62
Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKK 112
KR IR+ F K + KD I+IAR+PA +T + + SL+H+ + + ++ K
Sbjct: 63 KRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSLEHVLKIAKVFNK 114
>2LJP-A nrPDB
Length = 119
Score = 47.0 bits (110), Expect = 2e-06, Method: Compositional matrix adjust.
Identities = 34/114 (29%), Positives = 55/114 (48%), Gaps = 10/114 (8%)
Query: 6 RLKKNEDFQKVFKHGTSVANRQFV----LYTLDQPENDELRVGLSVSKK-IGNAVMRNRI 60
RL F VF+ Q L +L P R+GL+V+KK + A RNRI
Sbjct: 11 RLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGHP-----RIGLTVAKKNVRRAHERNRI 65
Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114
KRL R++F + L D++++A+K + L ++L+ L+R+ + S
Sbjct: 66 KRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALSEALEKLWRRHCRLARGS 119
>6CQC-A nrPDB
Length = 118
Score = 42.0 bits (97), Expect = 6e-05, Method: Compositional matrix adjust.
Identities = 27/76 (35%), Positives = 42/76 (55%), Gaps = 2/76 (2%)
Query: 16 VFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERL 75
+FK G S+ N FV+ + D R+G+ V +K G A RN++KR +R+ F K +
Sbjct: 21 IFKEGKSLQNEYFVV-LFRKNGLDYSRLGIVVKRKFGKATRRNKLKRWVREIFRRNKGVI 79
Query: 76 -KEKDYIIIARKPASQ 90
K D ++I RK S+
Sbjct: 80 PKGFDIVVIPRKKLSE 95
>1LPA-B nrPDB
Length = 449
Score = 27.7 bits (60), Expect = 1.3, Method: Composition-based stats.
Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Query: 25 NRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKE 73
N +F+LYT + P N + V S G+ NR R I F+++ E
Sbjct: 36 NTRFLLYTNENPNNFQ-EVAADSSSISGSNFKTNRKTRFIIHGFIDKGE 83
>4C2X-A nrPDB
Length = 410
Score = 27.3 bits (59), Expect = 1.4, Method: Compositional matrix adjust.
Identities = 14/39 (35%), Positives = 19/39 (48%)
Query: 7 LKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGL 45
L +N+ F + K G N Q+ LY P D +VGL
Sbjct: 369 LMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTDSEKVGL 407
>5HUF-A nrPDB
Length = 334
Score = 27.3 bits (59), Expect = 1.7, Method: Compositional matrix adjust.
Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 3/54 (5%)
Query: 63 LIRQAFLEEKERLKEKDYIIIARKPASQLTYEET---KKSLQHLFRKSSLYKKS 113
L+ +E R+ E YI+ PA+ L Y T + L+HL + + ++K+
Sbjct: 66 LLGNPMCDEFIRVPEWSYIVERANPANDLCYPGTLNDYEELKHLLSRINHFEKT 119
>1UJ5-A nrPDB
Length = 227
Score = 26.9 bits (58), Expect = 1.8, Method: Compositional matrix adjust.
Identities = 18/75 (24%), Positives = 35/75 (46%), Gaps = 6/75 (8%)
Query: 39 DELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPAS----QLTYE 94
DE+ GL++ K +G A++R +I + + F+ + K+ ++ R P Y
Sbjct: 89 DEIAPGLALIKGMGGALLREKIVERVAKEFIVIADHTKKVP--VLGRGPVPVEIVPFGYR 146
Query: 95 ETKKSLQHLFRKSSL 109
T K++ L + L
Sbjct: 147 ATLKAIADLGGEPEL 161
>4U0O-B nrPDB
Length = 296
Score = 26.9 bits (58), Expect = 2.2, Method: Compositional matrix adjust.
Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 2/48 (4%)
Query: 68 FLEEKERLKEKDYI--IIARKPASQLTYEETKKSLQHLFRKSSLYKKS 113
F ++ RL ++D I I+A +PA ET + LQ R+ + Y+ S
Sbjct: 142 FRMDRGRLSQRDCIAQIVAAQPACYNHNLETVRRLQGPVRRGATYESS 189
>2PVS-A nrPDB
Length = 452
Score = 26.2 bits (56), Expect = 3.6, Method: Composition-based stats.
Identities = 14/49 (28%), Positives = 23/49 (46%)
Query: 25 NRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKE 73
+ +F+LYT + P N +L G + +R R I FL++ E
Sbjct: 36 DTRFLLYTNENPNNFQLITGTEPDTIEASNFQLDRKTRFIIHGFLDKAE 84
>2QDX-A nrPDB
Length = 257
Score = 25.4 bits (54), Expect = 5.2, Method: Compositional matrix adjust.
Identities = 11/36 (30%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Query: 73 ERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSS 108
+ LKE D ++++RKP L +++ +HL+ S+
Sbjct: 80 QHLKEGDELMVSRKPTGTLVHDDLLPG-KHLYLLST 114
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
1A6F-A nrPDB 130 2e-31
1D6T-A nrPDB 129 4e-31
2LJP-A nrPDB 114 8e-27
6CQC-A nrPDB 80 3e-16
Sequences not found previously or not previously below threshold:
2JNF-A nrPDB 27 1.3
6EM1-t nrPDB 27 1.9
1WXQ-A nrPDB 27 2.2
4C65-A nrPDB 27 2.8
6B9O-A nrPDB 26 3.4
2YYU-A nrPDB 25 5.4
4GN1-A nrPDB 25 9.4
CONVERGED!
>1A6F-A nrPDB
Length = 119
Score = 130 bits (326), Expect = 2e-31, Method: Composition-based stats.
Identities = 115/116 (99%), Positives = 116/116 (100%)
Query: 1 MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60
+KKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI
Sbjct: 4 LKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 63
Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 116
KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK
Sbjct: 64 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 119
>1D6T-A nrPDB
Length = 117
Score = 129 bits (323), Expect = 4e-31, Method: Composition-based stats.
Identities = 55/112 (49%), Positives = 81/112 (72%)
Query: 1 MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60
++K R+KKN DFQ+++K G SVANRQFV+YT + E D R+G+SVSKK+GNAV+RN+I
Sbjct: 3 LEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFRLGISVSKKLGNAVLRNKI 62
Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKK 112
KR IR+ F K + KD I+IAR+PA +T + + SL+H+ + + ++ K
Sbjct: 63 KRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSLEHVLKIAKVFNK 114
>2LJP-A nrPDB
Length = 119
Score = 114 bits (286), Expect = 8e-27, Method: Composition-based stats.
Identities = 31/110 (28%), Positives = 52/110 (47%), Gaps = 2/110 (1%)
Query: 6 RLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKK-IGNAVMRNRIKRLI 64
RL F VF+ Q + R+GL+V+KK + A RNRIKRL
Sbjct: 11 RLLTPSQFTFVFQQPQRAGTPQITI-LGRLNSLGHPRIGLTVAKKNVRRAHERNRIKRLT 69
Query: 65 RQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114
R++F + L D++++A+K + L ++L+ L+R+ + S
Sbjct: 70 RESFRLRQHELPAMDFVVVAKKGVADLDNRALSEALEKLWRRHCRLARGS 119
>6CQC-A nrPDB
Length = 118
Score = 79.8 bits (195), Expect = 3e-16, Method: Composition-based stats.
Identities = 28/96 (29%), Positives = 50/96 (52%), Gaps = 6/96 (6%)
Query: 16 VFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERL 75
+FK G S+ N FV+ + D R+G+ V +K G A RN++KR +R+ F K +
Sbjct: 21 IFKEGKSLQNEYFVV-LFRKNGLDYSRLGIVVKRKFGKATRRNKLKRWVREIFRRNKGVI 79
Query: 76 K-EKDYIIIARKPASQ----LTYEETKKSLQHLFRK 106
D ++I RK S+ + + ++ L +L ++
Sbjct: 80 PKGFDIVVIPRKKLSEEFERVDFWTVREKLLNLLKR 115
>2JNF-A nrPDB
Length = 158
Score = 27.4 bits (59), Expect = 1.3, Method: Composition-based stats.
Identities = 9/71 (12%), Positives = 28/71 (39%)
Query: 33 LDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPASQLT 92
+ D + ++ +G V ++++ +R+AF + + R+ ++L
Sbjct: 62 FGNGDIDFDSFKIIGARFLGEEVNPEQMQQELREAFRLYDKEGNGYISTDVMREILAELD 121
Query: 93 YEETKKSLQHL 103
+ + L +
Sbjct: 122 ETLSSEDLDAM 132
>6EM1-t nrPDB
Length = 322
Score = 27.0 bits (58), Expect = 1.9, Method: Composition-based stats.
Identities = 17/81 (20%), Positives = 37/81 (45%), Gaps = 4/81 (4%)
Query: 6 RLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLI- 64
R ++N D ++ + + R+ + +N +R V+K + + + RIKR+
Sbjct: 21 RKRRNADRTRIERQELAKKKREEQIKKKRSNKNKFVRAESIVAKTLATSREKERIKRVSI 80
Query: 65 ---RQAFLEEKERLKEKDYII 82
++A E + KD+I+
Sbjct: 81 LEDKKAKNETQHIASGKDFIL 101
>1WXQ-A nrPDB
Length = 397
Score = 27.0 bits (58), Expect = 2.2, Method: Composition-based stats.
Identities = 13/55 (23%), Positives = 25/55 (45%), Gaps = 8/55 (14%)
Query: 45 LSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKS 99
+ ++ +A +IKRL+R+ YI+I A++LT + K+
Sbjct: 217 MVIAANKADAASDEQIKRLVREEEKR--------GYIVIPTSAAAELTLRKAAKA 263
>4C65-A nrPDB
Length = 438
Score = 26.6 bits (57), Expect = 2.8, Method: Composition-based stats.
Identities = 8/35 (22%), Positives = 17/35 (48%)
Query: 77 EKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYK 111
E D I + P + + K++ H+++ L+K
Sbjct: 388 EADVIALEENPLEDIKVFQEPKAVTHVWKGGKLFK 422
>6B9O-A nrPDB
Length = 981
Score = 26.2 bits (56), Expect = 3.4, Method: Composition-based stats.
Identities = 9/39 (23%), Positives = 17/39 (43%)
Query: 76 KEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114
D + AR + +KSL+ ++R S + S+
Sbjct: 161 LGFDSVHFARIDYQDREKRKAEKSLEVVWRGSKTFGSSA 199
>2YYU-A nrPDB
Length = 246
Score = 25.4 bits (54), Expect = 5.4, Method: Composition-based stats.
Identities = 11/34 (32%), Positives = 13/34 (38%)
Query: 71 EKERLKEKDYIIIARKPASQLTYEETKKSLQHLF 104
K R DYI+I R T LQH +
Sbjct: 202 RKARALGSDYIVIGRSLTRAADPLRTYARLQHEW 235
>4GN1-A nrPDB
Length = 255
Score = 24.7 bits (52), Expect = 9.4, Method: Composition-based stats.
Identities = 9/30 (30%), Positives = 13/30 (43%)
Query: 4 RNRLKKNEDFQKVFKHGTSVANRQFVLYTL 33
RN+ K D+ V KH Q++ Y
Sbjct: 192 RNKYKAPTDYCLVLKHPQIQKKSQYIKYLC 221
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.310 0.130 0.304
Lambda K H
0.267 0.0402 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,434,450
Number of Sequences: 36641
Number of extensions: 106992
Number of successful extensions: 403
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 372
Number of HSP's gapped (non-prelim): 39
length of query: 116
length of database: 10,336,785
effective HSP length: 81
effective length of query: 35
effective length of database: 7,368,864
effective search space: 257910240
effective search space used: 257910240
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.2 bits)
S2: 52 (24.7 bits)