BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P25814
         (116 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

1A6F-A nrPDB                                                          231   4e-62
1D6T-A nrPDB                                                          117   1e-27
2LJP-A nrPDB                                                           47   2e-06
6CQC-A nrPDB                                                           42   6e-05
1LPA-B nrPDB                                                           28   1.3  
4C2X-A nrPDB                                                           27   1.4  
5HUF-A nrPDB                                                           27   1.7  
1UJ5-A nrPDB                                                           27   1.8  
4U0O-B nrPDB                                                           27   2.2  
2PVS-A nrPDB                                                           26   3.6  
2QDX-A nrPDB                                                           25   5.2  

>1A6F-A nrPDB
          Length = 119

 Score =  231 bits (590), Expect = 4e-62,   Method: Compositional matrix adjust.
 Identities = 115/116 (99%), Positives = 116/116 (100%)

Query: 1   MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60
           +KKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI
Sbjct: 4   LKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 63

Query: 61  KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 116
           KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK
Sbjct: 64  KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 119


>1D6T-A nrPDB
          Length = 117

 Score =  117 bits (294), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 55/112 (49%), Positives = 81/112 (72%)

Query: 1   MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60
           ++K  R+KKN DFQ+++K G SVANRQFV+YT +  E D  R+G+SVSKK+GNAV+RN+I
Sbjct: 3   LEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFRLGISVSKKLGNAVLRNKI 62

Query: 61  KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKK 112
           KR IR+ F   K  +  KD I+IAR+PA  +T  + + SL+H+ + + ++ K
Sbjct: 63  KRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSLEHVLKIAKVFNK 114


>2LJP-A nrPDB
          Length = 119

 Score = 47.0 bits (110), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 34/114 (29%), Positives = 55/114 (48%), Gaps = 10/114 (8%)

Query: 6   RLKKNEDFQKVFKHGTSVANRQFV----LYTLDQPENDELRVGLSVSKK-IGNAVMRNRI 60
           RL     F  VF+        Q      L +L  P     R+GL+V+KK +  A  RNRI
Sbjct: 11  RLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGHP-----RIGLTVAKKNVRRAHERNRI 65

Query: 61  KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114
           KRL R++F   +  L   D++++A+K  + L      ++L+ L+R+     + S
Sbjct: 66  KRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALSEALEKLWRRHCRLARGS 119


>6CQC-A nrPDB
          Length = 118

 Score = 42.0 bits (97), Expect = 6e-05,   Method: Compositional matrix adjust.
 Identities = 27/76 (35%), Positives = 42/76 (55%), Gaps = 2/76 (2%)

Query: 16 VFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERL 75
          +FK G S+ N  FV+    +   D  R+G+ V +K G A  RN++KR +R+ F   K  +
Sbjct: 21 IFKEGKSLQNEYFVV-LFRKNGLDYSRLGIVVKRKFGKATRRNKLKRWVREIFRRNKGVI 79

Query: 76 -KEKDYIIIARKPASQ 90
           K  D ++I RK  S+
Sbjct: 80 PKGFDIVVIPRKKLSE 95


>1LPA-B nrPDB
          Length = 449

 Score = 27.7 bits (60), Expect = 1.3,   Method: Composition-based stats.
 Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 1/49 (2%)

Query: 25 NRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKE 73
          N +F+LYT + P N +  V    S   G+    NR  R I   F+++ E
Sbjct: 36 NTRFLLYTNENPNNFQ-EVAADSSSISGSNFKTNRKTRFIIHGFIDKGE 83


>4C2X-A nrPDB
          Length = 410

 Score = 27.3 bits (59), Expect = 1.4,   Method: Compositional matrix adjust.
 Identities = 14/39 (35%), Positives = 19/39 (48%)

Query: 7   LKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGL 45
           L +N+ F +  K G    N Q+ LY    P  D  +VGL
Sbjct: 369 LMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTDSEKVGL 407


>5HUF-A nrPDB
          Length = 334

 Score = 27.3 bits (59), Expect = 1.7,   Method: Compositional matrix adjust.
 Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 3/54 (5%)

Query: 63  LIRQAFLEEKERLKEKDYIIIARKPASQLTYEET---KKSLQHLFRKSSLYKKS 113
           L+     +E  R+ E  YI+    PA+ L Y  T    + L+HL  + + ++K+
Sbjct: 66  LLGNPMCDEFIRVPEWSYIVERANPANDLCYPGTLNDYEELKHLLSRINHFEKT 119


>1UJ5-A nrPDB
          Length = 227

 Score = 26.9 bits (58), Expect = 1.8,   Method: Compositional matrix adjust.
 Identities = 18/75 (24%), Positives = 35/75 (46%), Gaps = 6/75 (8%)

Query: 39  DELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPAS----QLTYE 94
           DE+  GL++ K +G A++R +I   + + F+   +  K+    ++ R P         Y 
Sbjct: 89  DEIAPGLALIKGMGGALLREKIVERVAKEFIVIADHTKKVP--VLGRGPVPVEIVPFGYR 146

Query: 95  ETKKSLQHLFRKSSL 109
            T K++  L  +  L
Sbjct: 147 ATLKAIADLGGEPEL 161


>4U0O-B nrPDB
          Length = 296

 Score = 26.9 bits (58), Expect = 2.2,   Method: Compositional matrix adjust.
 Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 2/48 (4%)

Query: 68  FLEEKERLKEKDYI--IIARKPASQLTYEETKKSLQHLFRKSSLYKKS 113
           F  ++ RL ++D I  I+A +PA      ET + LQ   R+ + Y+ S
Sbjct: 142 FRMDRGRLSQRDCIAQIVAAQPACYNHNLETVRRLQGPVRRGATYESS 189


>2PVS-A nrPDB
          Length = 452

 Score = 26.2 bits (56), Expect = 3.6,   Method: Composition-based stats.
 Identities = 14/49 (28%), Positives = 23/49 (46%)

Query: 25 NRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKE 73
          + +F+LYT + P N +L  G        +    +R  R I   FL++ E
Sbjct: 36 DTRFLLYTNENPNNFQLITGTEPDTIEASNFQLDRKTRFIIHGFLDKAE 84


>2QDX-A nrPDB
          Length = 257

 Score = 25.4 bits (54), Expect = 5.2,   Method: Compositional matrix adjust.
 Identities = 11/36 (30%), Positives = 22/36 (61%), Gaps = 1/36 (2%)

Query: 73  ERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSS 108
           + LKE D ++++RKP   L +++     +HL+  S+
Sbjct: 80  QHLKEGDELMVSRKPTGTLVHDDLLPG-KHLYLLST 114


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

1A6F-A nrPDB                                                          130   2e-31
1D6T-A nrPDB                                                          129   4e-31
2LJP-A nrPDB                                                          114   8e-27
6CQC-A nrPDB                                                           80   3e-16

Sequences not found previously or not previously below threshold:

2JNF-A nrPDB                                                           27   1.3  
6EM1-t nrPDB                                                           27   1.9  
1WXQ-A nrPDB                                                           27   2.2  
4C65-A nrPDB                                                           27   2.8  
6B9O-A nrPDB                                                           26   3.4  
2YYU-A nrPDB                                                           25   5.4  
4GN1-A nrPDB                                                           25   9.4  


CONVERGED!
>1A6F-A nrPDB
          Length = 119

 Score =  130 bits (326), Expect = 2e-31,   Method: Composition-based stats.
 Identities = 115/116 (99%), Positives = 116/116 (100%)

Query: 1   MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60
           +KKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI
Sbjct: 4   LKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 63

Query: 61  KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 116
           KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK
Sbjct: 64  KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 119


>1D6T-A nrPDB
          Length = 117

 Score =  129 bits (323), Expect = 4e-31,   Method: Composition-based stats.
 Identities = 55/112 (49%), Positives = 81/112 (72%)

Query: 1   MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60
           ++K  R+KKN DFQ+++K G SVANRQFV+YT +  E D  R+G+SVSKK+GNAV+RN+I
Sbjct: 3   LEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFRLGISVSKKLGNAVLRNKI 62

Query: 61  KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKK 112
           KR IR+ F   K  +  KD I+IAR+PA  +T  + + SL+H+ + + ++ K
Sbjct: 63  KRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSLEHVLKIAKVFNK 114


>2LJP-A nrPDB
          Length = 119

 Score =  114 bits (286), Expect = 8e-27,   Method: Composition-based stats.
 Identities = 31/110 (28%), Positives = 52/110 (47%), Gaps = 2/110 (1%)

Query: 6   RLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKK-IGNAVMRNRIKRLI 64
           RL     F  VF+        Q  +           R+GL+V+KK +  A  RNRIKRL 
Sbjct: 11  RLLTPSQFTFVFQQPQRAGTPQITI-LGRLNSLGHPRIGLTVAKKNVRRAHERNRIKRLT 69

Query: 65  RQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114
           R++F   +  L   D++++A+K  + L      ++L+ L+R+     + S
Sbjct: 70  RESFRLRQHELPAMDFVVVAKKGVADLDNRALSEALEKLWRRHCRLARGS 119


>6CQC-A nrPDB
          Length = 118

 Score = 79.8 bits (195), Expect = 3e-16,   Method: Composition-based stats.
 Identities = 28/96 (29%), Positives = 50/96 (52%), Gaps = 6/96 (6%)

Query: 16  VFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERL 75
           +FK G S+ N  FV+    +   D  R+G+ V +K G A  RN++KR +R+ F   K  +
Sbjct: 21  IFKEGKSLQNEYFVV-LFRKNGLDYSRLGIVVKRKFGKATRRNKLKRWVREIFRRNKGVI 79

Query: 76  K-EKDYIIIARKPASQ----LTYEETKKSLQHLFRK 106
               D ++I RK  S+    + +   ++ L +L ++
Sbjct: 80  PKGFDIVVIPRKKLSEEFERVDFWTVREKLLNLLKR 115


>2JNF-A nrPDB
          Length = 158

 Score = 27.4 bits (59), Expect = 1.3,   Method: Composition-based stats.
 Identities = 9/71 (12%), Positives = 28/71 (39%)

Query: 33  LDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPASQLT 92
               + D     +  ++ +G  V   ++++ +R+AF    +         + R+  ++L 
Sbjct: 62  FGNGDIDFDSFKIIGARFLGEEVNPEQMQQELREAFRLYDKEGNGYISTDVMREILAELD 121

Query: 93  YEETKKSLQHL 103
              + + L  +
Sbjct: 122 ETLSSEDLDAM 132


>6EM1-t nrPDB
          Length = 322

 Score = 27.0 bits (58), Expect = 1.9,   Method: Composition-based stats.
 Identities = 17/81 (20%), Positives = 37/81 (45%), Gaps = 4/81 (4%)

Query: 6   RLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLI- 64
           R ++N D  ++ +   +   R+  +      +N  +R    V+K +  +  + RIKR+  
Sbjct: 21  RKRRNADRTRIERQELAKKKREEQIKKKRSNKNKFVRAESIVAKTLATSREKERIKRVSI 80

Query: 65  ---RQAFLEEKERLKEKDYII 82
              ++A  E +     KD+I+
Sbjct: 81  LEDKKAKNETQHIASGKDFIL 101


>1WXQ-A nrPDB
          Length = 397

 Score = 27.0 bits (58), Expect = 2.2,   Method: Composition-based stats.
 Identities = 13/55 (23%), Positives = 25/55 (45%), Gaps = 8/55 (14%)

Query: 45  LSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKS 99
           + ++    +A    +IKRL+R+             YI+I    A++LT  +  K+
Sbjct: 217 MVIAANKADAASDEQIKRLVREEEKR--------GYIVIPTSAAAELTLRKAAKA 263


>4C65-A nrPDB
          Length = 438

 Score = 26.6 bits (57), Expect = 2.8,   Method: Composition-based stats.
 Identities = 8/35 (22%), Positives = 17/35 (48%)

Query: 77  EKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYK 111
           E D I +   P   +   +  K++ H+++   L+K
Sbjct: 388 EADVIALEENPLEDIKVFQEPKAVTHVWKGGKLFK 422


>6B9O-A nrPDB
          Length = 981

 Score = 26.2 bits (56), Expect = 3.4,   Method: Composition-based stats.
 Identities = 9/39 (23%), Positives = 17/39 (43%)

Query: 76  KEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114
              D +  AR         + +KSL+ ++R S  +  S+
Sbjct: 161 LGFDSVHFARIDYQDREKRKAEKSLEVVWRGSKTFGSSA 199


>2YYU-A nrPDB
          Length = 246

 Score = 25.4 bits (54), Expect = 5.4,   Method: Composition-based stats.
 Identities = 11/34 (32%), Positives = 13/34 (38%)

Query: 71  EKERLKEKDYIIIARKPASQLTYEETKKSLQHLF 104
            K R    DYI+I R          T   LQH +
Sbjct: 202 RKARALGSDYIVIGRSLTRAADPLRTYARLQHEW 235


>4GN1-A nrPDB
          Length = 255

 Score = 24.7 bits (52), Expect = 9.4,   Method: Composition-based stats.
 Identities = 9/30 (30%), Positives = 13/30 (43%)

Query: 4   RNRLKKNEDFQKVFKHGTSVANRQFVLYTL 33
           RN+ K   D+  V KH       Q++ Y  
Sbjct: 192 RNKYKAPTDYCLVLKHPQIQKKSQYIKYLC 221


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.310    0.130    0.304 

Lambda     K      H
   0.267   0.0402    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,434,450
Number of Sequences: 36641
Number of extensions: 106992
Number of successful extensions: 403
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 372
Number of HSP's gapped (non-prelim): 39
length of query: 116
length of database: 10,336,785
effective HSP length: 81
effective length of query: 35
effective length of database: 7,368,864
effective search space: 257910240
effective search space used: 257910240
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.2 bits)
S2: 52 (24.7 bits)