BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P25814 (116 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1A6F-A nrPDB 231 4e-62 1D6T-A nrPDB 117 1e-27 2LJP-A nrPDB 47 2e-06 6CQC-A nrPDB 42 6e-05 1LPA-B nrPDB 28 1.3 4C2X-A nrPDB 27 1.4 5HUF-A nrPDB 27 1.7 1UJ5-A nrPDB 27 1.8 4U0O-B nrPDB 27 2.2 2PVS-A nrPDB 26 3.6 2QDX-A nrPDB 25 5.2 >1A6F-A nrPDB Length = 119 Score = 231 bits (590), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 115/116 (99%), Positives = 116/116 (100%) Query: 1 MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60 +KKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI Sbjct: 4 LKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 63 Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 116 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK Sbjct: 64 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 119 >1D6T-A nrPDB Length = 117 Score = 117 bits (294), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 55/112 (49%), Positives = 81/112 (72%) Query: 1 MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60 ++K R+KKN DFQ+++K G SVANRQFV+YT + E D R+G+SVSKK+GNAV+RN+I Sbjct: 3 LEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFRLGISVSKKLGNAVLRNKI 62 Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKK 112 KR IR+ F K + KD I+IAR+PA +T + + SL+H+ + + ++ K Sbjct: 63 KRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSLEHVLKIAKVFNK 114 >2LJP-A nrPDB Length = 119 Score = 47.0 bits (110), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 34/114 (29%), Positives = 55/114 (48%), Gaps = 10/114 (8%) Query: 6 RLKKNEDFQKVFKHGTSVANRQFV----LYTLDQPENDELRVGLSVSKK-IGNAVMRNRI 60 RL F VF+ Q L +L P R+GL+V+KK + A RNRI Sbjct: 11 RLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGHP-----RIGLTVAKKNVRRAHERNRI 65 Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114 KRL R++F + L D++++A+K + L ++L+ L+R+ + S Sbjct: 66 KRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALSEALEKLWRRHCRLARGS 119 >6CQC-A nrPDB Length = 118 Score = 42.0 bits (97), Expect = 6e-05, Method: Compositional matrix adjust. Identities = 27/76 (35%), Positives = 42/76 (55%), Gaps = 2/76 (2%) Query: 16 VFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERL 75 +FK G S+ N FV+ + D R+G+ V +K G A RN++KR +R+ F K + Sbjct: 21 IFKEGKSLQNEYFVV-LFRKNGLDYSRLGIVVKRKFGKATRRNKLKRWVREIFRRNKGVI 79 Query: 76 -KEKDYIIIARKPASQ 90 K D ++I RK S+ Sbjct: 80 PKGFDIVVIPRKKLSE 95 >1LPA-B nrPDB Length = 449 Score = 27.7 bits (60), Expect = 1.3, Method: Composition-based stats. Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 1/49 (2%) Query: 25 NRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKE 73 N +F+LYT + P N + V S G+ NR R I F+++ E Sbjct: 36 NTRFLLYTNENPNNFQ-EVAADSSSISGSNFKTNRKTRFIIHGFIDKGE 83 >4C2X-A nrPDB Length = 410 Score = 27.3 bits (59), Expect = 1.4, Method: Compositional matrix adjust. Identities = 14/39 (35%), Positives = 19/39 (48%) Query: 7 LKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGL 45 L +N+ F + K G N Q+ LY P D +VGL Sbjct: 369 LMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTDSEKVGL 407 >5HUF-A nrPDB Length = 334 Score = 27.3 bits (59), Expect = 1.7, Method: Compositional matrix adjust. Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 3/54 (5%) Query: 63 LIRQAFLEEKERLKEKDYIIIARKPASQLTYEET---KKSLQHLFRKSSLYKKS 113 L+ +E R+ E YI+ PA+ L Y T + L+HL + + ++K+ Sbjct: 66 LLGNPMCDEFIRVPEWSYIVERANPANDLCYPGTLNDYEELKHLLSRINHFEKT 119 >1UJ5-A nrPDB Length = 227 Score = 26.9 bits (58), Expect = 1.8, Method: Compositional matrix adjust. Identities = 18/75 (24%), Positives = 35/75 (46%), Gaps = 6/75 (8%) Query: 39 DELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPAS----QLTYE 94 DE+ GL++ K +G A++R +I + + F+ + K+ ++ R P Y Sbjct: 89 DEIAPGLALIKGMGGALLREKIVERVAKEFIVIADHTKKVP--VLGRGPVPVEIVPFGYR 146 Query: 95 ETKKSLQHLFRKSSL 109 T K++ L + L Sbjct: 147 ATLKAIADLGGEPEL 161 >4U0O-B nrPDB Length = 296 Score = 26.9 bits (58), Expect = 2.2, Method: Compositional matrix adjust. Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 2/48 (4%) Query: 68 FLEEKERLKEKDYI--IIARKPASQLTYEETKKSLQHLFRKSSLYKKS 113 F ++ RL ++D I I+A +PA ET + LQ R+ + Y+ S Sbjct: 142 FRMDRGRLSQRDCIAQIVAAQPACYNHNLETVRRLQGPVRRGATYESS 189 >2PVS-A nrPDB Length = 452 Score = 26.2 bits (56), Expect = 3.6, Method: Composition-based stats. Identities = 14/49 (28%), Positives = 23/49 (46%) Query: 25 NRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKE 73 + +F+LYT + P N +L G + +R R I FL++ E Sbjct: 36 DTRFLLYTNENPNNFQLITGTEPDTIEASNFQLDRKTRFIIHGFLDKAE 84 >2QDX-A nrPDB Length = 257 Score = 25.4 bits (54), Expect = 5.2, Method: Compositional matrix adjust. Identities = 11/36 (30%), Positives = 22/36 (61%), Gaps = 1/36 (2%) Query: 73 ERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSS 108 + LKE D ++++RKP L +++ +HL+ S+ Sbjct: 80 QHLKEGDELMVSRKPTGTLVHDDLLPG-KHLYLLST 114 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1A6F-A nrPDB 130 2e-31 1D6T-A nrPDB 129 4e-31 2LJP-A nrPDB 114 8e-27 6CQC-A nrPDB 80 3e-16 Sequences not found previously or not previously below threshold: 2JNF-A nrPDB 27 1.3 6EM1-t nrPDB 27 1.9 1WXQ-A nrPDB 27 2.2 4C65-A nrPDB 27 2.8 6B9O-A nrPDB 26 3.4 2YYU-A nrPDB 25 5.4 4GN1-A nrPDB 25 9.4 CONVERGED! >1A6F-A nrPDB Length = 119 Score = 130 bits (326), Expect = 2e-31, Method: Composition-based stats. Identities = 115/116 (99%), Positives = 116/116 (100%) Query: 1 MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60 +KKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI Sbjct: 4 LKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 63 Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 116 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK Sbjct: 64 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK 119 >1D6T-A nrPDB Length = 117 Score = 129 bits (323), Expect = 4e-31, Method: Composition-based stats. Identities = 55/112 (49%), Positives = 81/112 (72%) Query: 1 MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRI 60 ++K R+KKN DFQ+++K G SVANRQFV+YT + E D R+G+SVSKK+GNAV+RN+I Sbjct: 3 LEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFRLGISVSKKLGNAVLRNKI 62 Query: 61 KRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKK 112 KR IR+ F K + KD I+IAR+PA +T + + SL+H+ + + ++ K Sbjct: 63 KRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSLEHVLKIAKVFNK 114 >2LJP-A nrPDB Length = 119 Score = 114 bits (286), Expect = 8e-27, Method: Composition-based stats. Identities = 31/110 (28%), Positives = 52/110 (47%), Gaps = 2/110 (1%) Query: 6 RLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKK-IGNAVMRNRIKRLI 64 RL F VF+ Q + R+GL+V+KK + A RNRIKRL Sbjct: 11 RLLTPSQFTFVFQQPQRAGTPQITI-LGRLNSLGHPRIGLTVAKKNVRRAHERNRIKRLT 69 Query: 65 RQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114 R++F + L D++++A+K + L ++L+ L+R+ + S Sbjct: 70 RESFRLRQHELPAMDFVVVAKKGVADLDNRALSEALEKLWRRHCRLARGS 119 >6CQC-A nrPDB Length = 118 Score = 79.8 bits (195), Expect = 3e-16, Method: Composition-based stats. Identities = 28/96 (29%), Positives = 50/96 (52%), Gaps = 6/96 (6%) Query: 16 VFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERL 75 +FK G S+ N FV+ + D R+G+ V +K G A RN++KR +R+ F K + Sbjct: 21 IFKEGKSLQNEYFVV-LFRKNGLDYSRLGIVVKRKFGKATRRNKLKRWVREIFRRNKGVI 79 Query: 76 K-EKDYIIIARKPASQ----LTYEETKKSLQHLFRK 106 D ++I RK S+ + + ++ L +L ++ Sbjct: 80 PKGFDIVVIPRKKLSEEFERVDFWTVREKLLNLLKR 115 >2JNF-A nrPDB Length = 158 Score = 27.4 bits (59), Expect = 1.3, Method: Composition-based stats. Identities = 9/71 (12%), Positives = 28/71 (39%) Query: 33 LDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPASQLT 92 + D + ++ +G V ++++ +R+AF + + R+ ++L Sbjct: 62 FGNGDIDFDSFKIIGARFLGEEVNPEQMQQELREAFRLYDKEGNGYISTDVMREILAELD 121 Query: 93 YEETKKSLQHL 103 + + L + Sbjct: 122 ETLSSEDLDAM 132 >6EM1-t nrPDB Length = 322 Score = 27.0 bits (58), Expect = 1.9, Method: Composition-based stats. Identities = 17/81 (20%), Positives = 37/81 (45%), Gaps = 4/81 (4%) Query: 6 RLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLI- 64 R ++N D ++ + + R+ + +N +R V+K + + + RIKR+ Sbjct: 21 RKRRNADRTRIERQELAKKKREEQIKKKRSNKNKFVRAESIVAKTLATSREKERIKRVSI 80 Query: 65 ---RQAFLEEKERLKEKDYII 82 ++A E + KD+I+ Sbjct: 81 LEDKKAKNETQHIASGKDFIL 101 >1WXQ-A nrPDB Length = 397 Score = 27.0 bits (58), Expect = 2.2, Method: Composition-based stats. Identities = 13/55 (23%), Positives = 25/55 (45%), Gaps = 8/55 (14%) Query: 45 LSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKS 99 + ++ +A +IKRL+R+ YI+I A++LT + K+ Sbjct: 217 MVIAANKADAASDEQIKRLVREEEKR--------GYIVIPTSAAAELTLRKAAKA 263 >4C65-A nrPDB Length = 438 Score = 26.6 bits (57), Expect = 2.8, Method: Composition-based stats. Identities = 8/35 (22%), Positives = 17/35 (48%) Query: 77 EKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYK 111 E D I + P + + K++ H+++ L+K Sbjct: 388 EADVIALEENPLEDIKVFQEPKAVTHVWKGGKLFK 422 >6B9O-A nrPDB Length = 981 Score = 26.2 bits (56), Expect = 3.4, Method: Composition-based stats. Identities = 9/39 (23%), Positives = 17/39 (43%) Query: 76 KEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSS 114 D + AR + +KSL+ ++R S + S+ Sbjct: 161 LGFDSVHFARIDYQDREKRKAEKSLEVVWRGSKTFGSSA 199 >2YYU-A nrPDB Length = 246 Score = 25.4 bits (54), Expect = 5.4, Method: Composition-based stats. Identities = 11/34 (32%), Positives = 13/34 (38%) Query: 71 EKERLKEKDYIIIARKPASQLTYEETKKSLQHLF 104 K R DYI+I R T LQH + Sbjct: 202 RKARALGSDYIVIGRSLTRAADPLRTYARLQHEW 235 >4GN1-A nrPDB Length = 255 Score = 24.7 bits (52), Expect = 9.4, Method: Composition-based stats. Identities = 9/30 (30%), Positives = 13/30 (43%) Query: 4 RNRLKKNEDFQKVFKHGTSVANRQFVLYTL 33 RN+ K D+ V KH Q++ Y Sbjct: 192 RNKYKAPTDYCLVLKHPQIQKKSQYIKYLC 221 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.310 0.130 0.304 Lambda K H 0.267 0.0402 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,434,450 Number of Sequences: 36641 Number of extensions: 106992 Number of successful extensions: 403 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 24 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 372 Number of HSP's gapped (non-prelim): 39 length of query: 116 length of database: 10,336,785 effective HSP length: 81 effective length of query: 35 effective length of database: 7,368,864 effective search space: 257910240 effective search space used: 257910240 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.2 bits) S2: 52 (24.7 bits)