BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P25651 (190 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3N4X-A nrPDB 385 e-108 5V1A-A nrPDB 254 2e-68 6MJC-A nrPDB 163 4e-41 6MJE-A nrPDB 163 5e-41 6ED3-A nrPDB 32 0.21 4WTQ-A nrPDB 27 4.5 2DH2-A nrPDB 27 5.5 6IRT-A nrPDB 26 9.0 >3N4X-A nrPDB Length = 190 Score = 385 bits (988), Expect = e-108, Method: Compositional matrix adjust. Identities = 190/190 (100%), Positives = 190/190 (100%) Query: 1 MDPLTVYKNSVKQQIDSADLLVANLVNENFVLSEKLDTKATEIKQLQKQIDSLNAQVKEL 60 MDPLTVYKNSVKQQIDSADLLVANLVNENFVLSEKLDTKATEIKQLQKQIDSLNAQVKEL Sbjct: 1 MDPLTVYKNSVKQQIDSADLLVANLVNENFVLSEKLDTKATEIKQLQKQIDSLNAQVKEL 60 Query: 61 KTQTSQQAENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKL 120 KTQTSQQAENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKL Sbjct: 61 KTQTSQQAENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKL 120 Query: 121 GFVKGQAQVTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLN 180 GFVKGQAQVTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLN Sbjct: 121 GFVKGQAQVTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLN 180 Query: 181 KKREKKDETE 190 KKREKKDETE Sbjct: 181 KKREKKDETE 190 >5V1A-A nrPDB Length = 122 Score = 254 bits (648), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 122/122 (100%), Positives = 122/122 (100%) Query: 69 ENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQAQ 128 ENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQAQ Sbjct: 1 ENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQAQ 60 Query: 129 VTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKKREKKDE 188 VTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKKREKKDE Sbjct: 61 VTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKKREKKDE 120 Query: 189 TE 190 TE Sbjct: 121 TE 122 >6MJC-A nrPDB Length = 116 Score = 163 bits (412), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 80/120 (66%), Positives = 94/120 (78%), Gaps = 12/120 (10%) Query: 68 AENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQA 127 AE E+IKDL+E+LC VRVH++YEDD+GLWFD SQG+ +G IMDYKLGFVK QA Sbjct: 3 AETIEIIKDLFEHLCGVRVHRTYEDDTGLWFDTSQGSKNG-------IMDYKLGFVKSQA 55 Query: 128 QVT-----EVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKK 182 + T EVIY P+LKQR+ EEL LQ KLP+YLFETLSFPL SLNQFY K++KSLNKK Sbjct: 56 ENTTEVDTEVIYVPLLKQRTAEELQELQKKLPDYLFETLSFPLRSLNQFYIKMSKSLNKK 115 >6MJE-A nrPDB Length = 132 Score = 163 bits (412), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 80/120 (66%), Positives = 94/120 (78%), Gaps = 12/120 (10%) Query: 68 AENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQA 127 AE E+IKDL+E+LC VRVH++YEDD+GLWFD SQG+ +G IMDYKLGFVK QA Sbjct: 19 AETIEIIKDLFEHLCGVRVHRTYEDDTGLWFDTSQGSKNG-------IMDYKLGFVKSQA 71 Query: 128 QVT-----EVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKK 182 + T EVIY P+LKQR+ EEL LQ KLP+YLFETLSFPL SLNQFY K++KSLNKK Sbjct: 72 ENTTEVDTEVIYVPLLKQRTAEELQELQKKLPDYLFETLSFPLRSLNQFYIKMSKSLNKK 131 >6ED3-A nrPDB Length = 848 Score = 31.6 bits (70), Expect = 0.21, Method: Composition-based stats. Identities = 20/73 (27%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Query: 14 QIDSADLLVANLVNENFVLSEKLDTKATE-IKQLQKQIDSLNAQVKELKTQTSQQAENSE 72 +ID + LV L E LS++ D + E + +L+ ++ ++ EL T+ + E Sbjct: 412 EIDEVERLVRRLEIEEMALSKEEDEASAERLAKLRSELADQKEKLAELTTRWQNEKNAIE 471 Query: 73 VIKDLYEYLCNVR 85 +++DL E L +R Sbjct: 472 IVRDLKEQLEALR 484 >4WTQ-A nrPDB Length = 293 Score = 27.3 bits (59), Expect = 4.5, Method: Compositional matrix adjust. Identities = 13/46 (28%), Positives = 23/46 (50%) Query: 101 SQGTHSGGSSDDYSIMDYKLGFVKGQAQVTEVIYAPVLKQRSTEEL 146 S GT GS+ + D+ +G G + + +Y PV+ +R + L Sbjct: 106 SDGTWRAGSAGYWHYHDHVVGTEHGTGGIRKGLYGPVIVRRKGDVL 151 >2DH2-A nrPDB Length = 424 Score = 26.9 bits (58), Expect = 5.5, Method: Compositional matrix adjust. Identities = 23/78 (29%), Positives = 36/78 (46%), Gaps = 10/78 (12%) Query: 8 KNSVKQQIDSADLLVANLVNENFVLSEKLDTKATEIK-----QLQKQIDSLNAQ-VKELK 61 K S++ +D L N EN S ++DT AT++K LQ +D + ++ LK Sbjct: 93 KKSIRVILD----LTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLK 148 Query: 62 TQTSQQAENSEVIKDLYE 79 +S AE + K E Sbjct: 149 DASSFLAEWQNITKGFSE 166 >6IRT-A nrPDB Length = 647 Score = 26.2 bits (56), Expect = 9.0, Method: Compositional matrix adjust. Identities = 23/78 (29%), Positives = 36/78 (46%), Gaps = 10/78 (12%) Query: 8 KNSVKQQIDSADLLVANLVNENFVLSEKLDTKATEIKQ-----LQKQIDSLNAQ-VKELK 61 K S++ +D L N EN S ++DT AT++K LQ +D + ++ LK Sbjct: 314 KKSIRVILD----LTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLK 369 Query: 62 TQTSQQAENSEVIKDLYE 79 +S AE + K E Sbjct: 370 DASSFLAEWQNITKGFSE 387 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3N4X-A nrPDB 325 6e-90 5V1A-A nrPDB 219 7e-58 6MJC-A nrPDB 188 2e-48 6MJE-A nrPDB 185 8e-48 Sequences not found previously or not previously below threshold: 6ED3-A nrPDB 37 0.005 3KZW-A nrPDB 28 2.4 3G6B-A nrPDB 27 4.2 1UDQ-A nrPDB 27 4.4 5TK5-A nrPDB 27 4.6 6FTJ-1 nrPDB 27 5.6 4RSI-A nrPDB 26 8.5 CONVERGED! >3N4X-A nrPDB Length = 190 Score = 325 bits (833), Expect = 6e-90, Method: Composition-based stats. Identities = 190/190 (100%), Positives = 190/190 (100%) Query: 1 MDPLTVYKNSVKQQIDSADLLVANLVNENFVLSEKLDTKATEIKQLQKQIDSLNAQVKEL 60 MDPLTVYKNSVKQQIDSADLLVANLVNENFVLSEKLDTKATEIKQLQKQIDSLNAQVKEL Sbjct: 1 MDPLTVYKNSVKQQIDSADLLVANLVNENFVLSEKLDTKATEIKQLQKQIDSLNAQVKEL 60 Query: 61 KTQTSQQAENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKL 120 KTQTSQQAENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKL Sbjct: 61 KTQTSQQAENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKL 120 Query: 121 GFVKGQAQVTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLN 180 GFVKGQAQVTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLN Sbjct: 121 GFVKGQAQVTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLN 180 Query: 181 KKREKKDETE 190 KKREKKDETE Sbjct: 181 KKREKKDETE 190 >5V1A-A nrPDB Length = 122 Score = 219 bits (557), Expect = 7e-58, Method: Composition-based stats. Identities = 122/122 (100%), Positives = 122/122 (100%) Query: 69 ENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQAQ 128 ENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQAQ Sbjct: 1 ENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQAQ 60 Query: 129 VTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKKREKKDE 188 VTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKKREKKDE Sbjct: 61 VTEVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKKREKKDE 120 Query: 189 TE 190 TE Sbjct: 121 TE 122 >6MJC-A nrPDB Length = 116 Score = 188 bits (476), Expect = 2e-48, Method: Composition-based stats. Identities = 80/120 (66%), Positives = 94/120 (78%), Gaps = 12/120 (10%) Query: 68 AENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQA 127 AE E+IKDL+E+LC VRVH++YEDD+GLWFD SQG+ +G IMDYKLGFVK QA Sbjct: 3 AETIEIIKDLFEHLCGVRVHRTYEDDTGLWFDTSQGSKNG-------IMDYKLGFVKSQA 55 Query: 128 QVT-----EVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKK 182 + T EVIY P+LKQR+ EEL LQ KLP+YLFETLSFPL SLNQFY K++KSLNKK Sbjct: 56 ENTTEVDTEVIYVPLLKQRTAEELQELQKKLPDYLFETLSFPLRSLNQFYIKMSKSLNKK 115 >6MJE-A nrPDB Length = 132 Score = 185 bits (470), Expect = 8e-48, Method: Composition-based stats. Identities = 80/120 (66%), Positives = 94/120 (78%), Gaps = 12/120 (10%) Query: 68 AENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQA 127 AE E+IKDL+E+LC VRVH++YEDD+GLWFD SQG+ +G IMDYKLGFVK QA Sbjct: 19 AETIEIIKDLFEHLCGVRVHRTYEDDTGLWFDTSQGSKNG-------IMDYKLGFVKSQA 71 Query: 128 QVT-----EVIYAPVLKQRSTEELYSLQSKLPEYLFETLSFPLSSLNQFYNKIAKSLNKK 182 + T EVIY P+LKQR+ EEL LQ KLP+YLFETLSFPL SLNQFY K++KSLNKK Sbjct: 72 ENTTEVDTEVIYVPLLKQRTAEELQELQKKLPDYLFETLSFPLRSLNQFYIKMSKSLNKK 131 >6ED3-A nrPDB Length = 848 Score = 37.0 bits (84), Expect = 0.005, Method: Composition-based stats. Identities = 20/73 (27%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Query: 14 QIDSADLLVANLVNENFVLSEKLDTKATE-IKQLQKQIDSLNAQVKELKTQTSQQAENSE 72 +ID + LV L E LS++ D + E + +L+ ++ ++ EL T+ + E Sbjct: 412 EIDEVERLVRRLEIEEMALSKEEDEASAERLAKLRSELADQKEKLAELTTRWQNEKNAIE 471 Query: 73 VIKDLYEYLCNVR 85 +++DL E L +R Sbjct: 472 IVRDLKEQLEALR 484 >3KZW-A nrPDB Length = 515 Score = 28.2 bits (61), Expect = 2.4, Method: Composition-based stats. Identities = 29/106 (27%), Positives = 49/106 (46%), Gaps = 15/106 (14%) Query: 88 KSYEDDSGLWFDISQGTHSGGSSDDYSIMDYKLGFVKGQAQVTEVIYA-PVLKQRSTEEL 146 +SY+D +W + Q S D Y +MD F+ Q+++V+ A + +R+T E Sbjct: 106 RSYQDMLKIWGHLFQYIKSEHIEDTYLLMD---SFISKYDQLSDVLMACGIQSERATYEF 162 Query: 147 --YSLQSKLPEYLFET----LSFPLSSLNQFY--NKIAKSLNKKRE 184 Y K P F+T +S L L+ + I +S+N R+ Sbjct: 163 DHYKSSKKAP---FKTNLNLISESLIELDFIHEGISIGQSINLARD 205 >3G6B-A nrPDB Length = 213 Score = 27.4 bits (59), Expect = 4.2, Method: Composition-based stats. Identities = 34/155 (21%), Positives = 69/155 (44%), Gaps = 15/155 (9%) Query: 42 EIKQLQKQIDSLNAQVKELKTQTSQQAENSEVIKDLYEYLCNVRVHKSYEDDSGLWFDIS 101 E+K+ +D + +VKE +Q + EN I+ L + + +R+ + E + Sbjct: 47 ELKKSGTNVDQIVERVKEASSQIGETLENIRSIEKLIQNI--MRIAR--ETNILALNATI 102 Query: 102 QGTHSGGSSDDYSIMDYKLGFVKGQA-QVTEVIYAPVLKQRSTEELYSLQSKLPEY---- 156 + +G + + I+ ++ + + +VT+ I +++ E L S Q L Sbjct: 103 EAARAGEAGKGFMIVANEVQNLSNETNEVTKQIV-----EKAREILESSQRSLENLEFMA 157 Query: 157 -LFETLSFPLSSLNQFYNKIAKSLNKKREKKDETE 190 LFET+ L ++ +F I K L + R D ++ Sbjct: 158 NLFETVGKTLQNMVRFMENIVKLLQEVRNSLDTSK 192 >1UDQ-A nrPDB Length = 255 Score = 27.4 bits (59), Expect = 4.4, Method: Composition-based stats. Identities = 22/66 (33%), Positives = 33/66 (50%), Gaps = 7/66 (10%) Query: 89 SYEDDSGLWFDISQ-GTHSGGSSDDYSIMDYKLGFVKGQAQVTEVIYAPVLKQRSTEELY 147 ++E+DS D++ GT SG S+ ++ M + F K E+I L Q+ EL Sbjct: 176 NFEEDSAAQVDMNVVGTGSGRLSEVHT-MGEEYSFTKD-----ELIKMLDLAQKGINELI 229 Query: 148 SLQSKL 153 LQ KL Sbjct: 230 ELQKKL 235 >5TK5-A nrPDB Length = 290 Score = 27.0 bits (58), Expect = 4.6, Method: Composition-based stats. Identities = 10/33 (30%), Positives = 19/33 (57%) Query: 135 APVLKQRSTEELYSLQSKLPEYLFETLSFPLSS 167 AP++++ + E Y +P+ L + FPLS+ Sbjct: 146 APIIQEFFSSEQYRTGKPIPDQLLKEPPFPLST 178 >6FTJ-1 nrPDB Length = 162 Score = 27.0 bits (58), Expect = 5.6, Method: Composition-based stats. Identities = 14/45 (31%), Positives = 27/45 (60%) Query: 25 LVNENFVLSEKLDTKATEIKQLQKQIDSLNAQVKELKTQTSQQAE 69 L +E L +L T+ +++ ++ L+AQVKEL +++ +AE Sbjct: 89 LTSEIASLQSRLKTEGSDLCDKVSEMQKLDAQVKELVLKSAVEAE 133 >4RSI-A nrPDB Length = 397 Score = 26.2 bits (56), Expect = 8.5, Method: Composition-based stats. Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 6/46 (13%) Query: 33 SEKLDTKATEIKQLQKQIDSLNAQV----KELKTQ--TSQQAENSE 72 SE L + Q+QKQI+++ A + +EL+TQ TSQ+ + + Sbjct: 278 SESLLVDIQKYNQIQKQIETIQADLNHVTEELQTQYATSQKTKTIQ 323 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.310 0.129 0.347 Lambda K H 0.267 0.0402 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,579,742 Number of Sequences: 36641 Number of extensions: 237757 Number of successful extensions: 774 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 22 Number of HSP's successfully gapped in prelim test: 24 Number of HSP's that attempted gapping in prelim test: 742 Number of HSP's gapped (non-prelim): 48 length of query: 190 length of database: 10,336,785 effective HSP length: 92 effective length of query: 98 effective length of database: 6,965,813 effective search space: 682649674 effective search space used: 682649674 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 56 (26.2 bits)