BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P25441 (422 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6CNC-N nrPDB 845 0.0 1Z7Z-1 nrPDB 31 1.0 1OGP-A nrPDB 30 2.2 3LHK-A nrPDB 30 2.4 1B65-A nrPDB 29 3.3 4AU8-A nrPDB 29 3.5 >6CNC-N nrPDB Length = 422 Score = 845 bits (2184), Expect = 0.0, Method: Compositional matrix adjust. Identities = 422/422 (100%), Positives = 422/422 (100%) Query: 1 MSSNKGNGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGN 60 MSSNKGNGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGN Sbjct: 1 MSSNKGNGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGN 60 Query: 61 DKKHFNNKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSG 120 DKKHFNNKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSG Sbjct: 61 DKKHFNNKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSG 120 Query: 121 SSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEK 180 SSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEK Sbjct: 121 SSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEK 180 Query: 181 SSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPV 240 SSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPV Sbjct: 181 SSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPV 240 Query: 241 GTGLQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNK 300 GTGLQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNK Sbjct: 241 GTGLQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNK 300 Query: 301 PERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKV 360 PERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKV Sbjct: 301 PERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKV 360 Query: 361 GSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKIVVTP 420 GSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKIVVTP Sbjct: 361 GSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKIVVTP 420 Query: 421 QI 422 QI Sbjct: 421 QI 422 >1Z7Z-1 nrPDB Length = 286 Score = 31.2 bits (69), Expect = 1.0, Method: Compositional matrix adjust. Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 9/82 (10%) Query: 10 LPSLKDSSSNGGGSAKPSLKFKPKAVA-RKSKEE--------REAAASKVKLEEESKRGN 60 +P+L + G A PS + + V K++ E R A + + L SK G Sbjct: 36 VPALTAVETGASGQAIPSDVVETRHVVNYKTRSESCLESFFGRAACVTILSLTNSSKSGE 95 Query: 61 DKKHFNNKNKRVTGAGGQQRRM 82 +KKHFN N T +R++ Sbjct: 96 EKKHFNIWNITYTDTVQLRRKL 117 >1OGP-A nrPDB Length = 393 Score = 30.0 bits (66), Expect = 2.2, Method: Compositional matrix adjust. Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 6/61 (9%) Query: 162 KEFEARNLIEDEDDGESEK------SSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETV 215 K ++ N+I +E G + V+ D+ W S+R + FPV+ EDV+ V Sbjct: 220 KWLDSINVIAEESQGFFMQKDYKMFPPSVNWDNINWSSRRPQMDFPVQSAICSVEDVQMV 279 Query: 216 K 216 K Sbjct: 280 K 280 >3LHK-A nrPDB Length = 154 Score = 29.6 bits (65), Expect = 2.4, Method: Compositional matrix adjust. Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 2/84 (2%) Query: 297 MNNKPERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALS-TRE 355 MN K E+ ++ P RL F +KE + T+ +KK K +++ +D ++ Sbjct: 63 MNRKVEK-VIIAYPDRLTRFGFETLKEFFKSYGTEIVIINKKHKTPQEELVEDLITIVSH 121 Query: 356 LAGKVGSIRVHKSGKLSVKIGNVV 379 AGK+ + HK KL+ + +V Sbjct: 122 FAGKLYGMHSHKYKKLTKTVKEIV 145 >1B65-A nrPDB Length = 375 Score = 29.3 bits (64), Expect = 3.3, Method: Compositional matrix adjust. Identities = 16/54 (29%), Positives = 25/54 (46%) Query: 92 ISSGPLAAGNFVSEKGDLRRGFIKSEGSGSSLVQKGLETIDNGAESSENEAEDD 145 ++SGP+ GN G + GF G+ S +V+ G + GA N + D Sbjct: 170 VASGPVQEGNCGGGTGMITYGFKGGTGTASRVVEFGGRSFTIGALVQANHGQRD 223 >4AU8-A nrPDB Length = 296 Score = 29.3 bits (64), Expect = 3.5, Method: Compositional matrix adjust. Identities = 13/37 (35%), Positives = 23/37 (62%) Query: 144 DDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEK 180 DDD+EGV S + ++ + KE + +N++ D S+K Sbjct: 42 DDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDK 78 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6CNC-N nrPDB 778 0.0 Sequences not found previously or not previously below threshold: 3OQJ-A nrPDB 33 0.20 3PUK-A nrPDB 32 0.63 1B65-A nrPDB 31 1.5 4AU8-A nrPDB 30 1.7 6CMJ-A nrPDB 29 2.8 5YV8-A nrPDB 29 2.8 5EW5-A nrPDB 28 7.7 6FAI-l nrPDB 28 8.2 5AN3-D nrPDB 28 8.4 1NEX-A nrPDB 28 8.4 6GSA-C nrPDB 28 10.0 CONVERGED! >6CNC-N nrPDB Length = 422 Score = 778 bits (2008), Expect = 0.0, Method: Composition-based stats. Identities = 422/422 (100%), Positives = 422/422 (100%) Query: 1 MSSNKGNGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGN 60 MSSNKGNGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGN Sbjct: 1 MSSNKGNGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGN 60 Query: 61 DKKHFNNKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSG 120 DKKHFNNKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSG Sbjct: 61 DKKHFNNKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSG 120 Query: 121 SSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEK 180 SSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEK Sbjct: 121 SSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEK 180 Query: 181 SSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPV 240 SSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPV Sbjct: 181 SSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPV 240 Query: 241 GTGLQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNK 300 GTGLQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNK Sbjct: 241 GTGLQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNK 300 Query: 301 PERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKV 360 PERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKV Sbjct: 301 PERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKV 360 Query: 361 GSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKIVVTP 420 GSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKIVVTP Sbjct: 361 GSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKIVVTP 420 Query: 421 QI 422 QI Sbjct: 421 QI 422 >3OQJ-A nrPDB Length = 257 Score = 33.2 bits (74), Expect = 0.20, Method: Composition-based stats. Identities = 19/43 (44%), Positives = 24/43 (55%), Gaps = 4/43 (9%) Query: 266 KEFQSVD----GKEAAAELELLNADHQHILRKLKKMNNKPERF 304 +EFQSV GKEAA LE L H RK++K ++ RF Sbjct: 57 REFQSVSVLLAGKEAANLLEALGTPHGKAERKVRKEVSRNRRF 99 >3PUK-A nrPDB Length = 592 Score = 31.7 bits (70), Expect = 0.63, Method: Composition-based stats. Identities = 23/94 (24%), Positives = 43/94 (45%), Gaps = 15/94 (15%) Query: 175 DGESEKSSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSE-KPTREPTP 233 DG+ EK + ++ DD+ W VRVRH + V EI + + E T++ T Sbjct: 274 DGK-EKEAVLEEDDDLW-------------VRVRHRHIAVVLEEIPKLMKEISSTKKATE 319 Query: 234 SVKTEPVGTGLQSYLEERERQVNEKLADLGLEKE 267 + T L + +Q+++++ L L ++ Sbjct: 320 GKTSLSALTQLMKKMPHFRKQISKQVVHLNLAED 353 >1B65-A nrPDB Length = 375 Score = 30.5 bits (67), Expect = 1.5, Method: Composition-based stats. Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 3/62 (4%) Query: 84 KYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSGSSLVQKGLETIDNGAESSENEAE 143 K L+N ++SGP+ GN G + GF G+ S +V+ G + GA N + Sbjct: 165 KALDN---VASGPVQEGNCGGGTGMITYGFKGGTGTASRVVEFGGRSFTIGALVQANHGQ 221 Query: 144 DD 145 D Sbjct: 222 RD 223 >4AU8-A nrPDB Length = 296 Score = 30.1 bits (66), Expect = 1.7, Method: Composition-based stats. Identities = 13/37 (35%), Positives = 23/37 (62%) Query: 144 DDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEK 180 DDD+EGV S + ++ + KE + +N++ D S+K Sbjct: 42 DDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDK 78 >6CMJ-A nrPDB Length = 321 Score = 29.3 bits (64), Expect = 2.8, Method: Composition-based stats. Identities = 13/44 (29%), Positives = 26/44 (59%), Gaps = 2/44 (4%) Query: 286 DHQHILRKLKKMN--NKPERFMVFQLPTRLPAFERPAVKEEKED 327 DH ++++ ++ ++ N+ +MVF+L + P E P +K ED Sbjct: 100 DHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPTLKPLSED 143 >5YV8-A nrPDB Length = 298 Score = 29.3 bits (64), Expect = 2.8, Method: Composition-based stats. Identities = 13/44 (29%), Positives = 26/44 (59%), Gaps = 2/44 (4%) Query: 286 DHQHILRKLKKMN--NKPERFMVFQLPTRLPAFERPAVKEEKED 327 DH ++++ ++ ++ N+ +MVF+L + P E P +K ED Sbjct: 94 DHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPTLKPLSED 137 >5EW5-A nrPDB Length = 582 Score = 28.2 bits (61), Expect = 7.7, Method: Composition-based stats. Identities = 37/174 (21%), Positives = 65/174 (37%), Gaps = 18/174 (10%) Query: 188 DEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPVGTGLQSY 247 DEE ++R ++ PV + E + E+ +A +E R K V +S Sbjct: 302 DEE--NRRQQEWDATHPVEAAERNCERARAELNQA-NEDVARNQERQAKAVQVYNSRKSE 358 Query: 248 LEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNKPERFMVF 307 L+ + + + +A++ K+F A + R +NNK Sbjct: 359 LDAANKTLADAIAEI---KQFNRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQ------ 409 Query: 308 QLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKVG 361 AF+ A ++ D A+ +K+K K+KD KD + K G Sbjct: 410 ------AAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKCAMESKRNKPG 457 >6FAI-l nrPDB Length = 425 Score = 27.8 bits (60), Expect = 8.2, Method: Composition-based stats. Identities = 17/74 (22%), Positives = 31/74 (41%) Query: 117 EGSGSSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDG 176 + SG S G ++ ES N + D++ +++++ G E + E D Sbjct: 326 QASGFSKKHPGDRGLETAVESMRNAVYNSDDDMSNDEAEEENGEGDYSEEDEYYDSELDN 385 Query: 177 ESEKSSDVDMDDEE 190 ES + D +EE Sbjct: 386 ESSEDDSEDAQEEE 399 >5AN3-D nrPDB Length = 131 Score = 27.8 bits (60), Expect = 8.4, Method: Composition-based stats. Identities = 13/31 (41%), Positives = 18/31 (58%) Query: 172 DEDDGESEKSSDVDMDDEEWRSKRIEQLFPV 202 DEDD +S KS+ VD D E+ E L+ + Sbjct: 77 DEDDDDSRKSAPVDSWDREFLKVDQEMLYEI 107 >1NEX-A nrPDB Length = 169 Score = 27.8 bits (60), Expect = 8.4, Method: Composition-based stats. Identities = 13/31 (41%), Positives = 18/31 (58%) Query: 172 DEDDGESEKSSDVDMDDEEWRSKRIEQLFPV 202 DEDD +S KS+ VD D E+ E L+ + Sbjct: 79 DEDDDDSRKSAPVDSWDREFLKVDQEMLYEI 109 >6GSA-C nrPDB Length = 197 Score = 27.8 bits (60), Expect = 10.0, Method: Composition-based stats. Identities = 13/31 (41%), Positives = 18/31 (58%) Query: 172 DEDDGESEKSSDVDMDDEEWRSKRIEQLFPV 202 DEDD +S KS+ VD D E+ E L+ + Sbjct: 105 DEDDDDSRKSAPVDSWDREFLKVDQEMLYEI 135 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.306 0.126 0.335 Lambda K H 0.267 0.0394 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,133,339 Number of Sequences: 36641 Number of extensions: 667884 Number of successful extensions: 1684 Number of sequences better than 10.0: 43 Number of HSP's better than 10.0 without gapping: 36 Number of HSP's successfully gapped in prelim test: 83 Number of HSP's that attempted gapping in prelim test: 1483 Number of HSP's gapped (non-prelim): 240 length of query: 422 length of database: 10,336,785 effective HSP length: 100 effective length of query: 322 effective length of database: 6,672,685 effective search space: 2148604570 effective search space used: 2148604570 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (22.0 bits) S2: 60 (27.8 bits)