BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P24005 (295 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4X3N-A nrPDB 615 e-176 1QX2-A nrPDB 32 0.29 1V1S-A nrPDB 28 3.4 1KQV-A nrPDB 28 4.1 1BOD-A nrPDB 28 4.8 1YS4-A nrPDB 28 5.9 >4X3N-A nrPDB Length = 295 Score = 615 bits (1585), Expect = e-176, Method: Compositional matrix adjust. Identities = 295/295 (100%), Positives = 295/295 (100%) Query: 1 MAETKVAPNLTGIEQTKAGQSFTEKLSAEAMEFFCNVAKLPFSQQAVHFLNAYWAEVSKE 60 MAETKVAPNLTGIEQTKAGQSFTEKLSAEAMEFFCNVAKLPFSQQAVHFLNAYWAEVSKE Sbjct: 1 MAETKVAPNLTGIEQTKAGQSFTEKLSAEAMEFFCNVAKLPFSQQAVHFLNAYWAEVSKE 60 Query: 61 AEFIYSVGWETIKYADMHCKGIQLVFKYDEGNDLDFDIALYFYEQLCKFCEDPKNKNYAT 120 AEFIYSVGWETIKYADMHCKGIQLVFKYDEGNDLDFDIALYFYEQLCKFCEDPKNKNYAT Sbjct: 61 AEFIYSVGWETIKYADMHCKGIQLVFKYDEGNDLDFDIALYFYEQLCKFCEDPKNKNYAT 120 Query: 121 TYPISQPQMLTALKRKQELREKVDVNFDGRVSFLEYLLYQYKDFANPADFCTRSMNHDEH 180 TYPISQPQMLTALKRKQELREKVDVNFDGRVSFLEYLLYQYKDFANPADFCTRSMNHDEH Sbjct: 121 TYPISQPQMLTALKRKQELREKVDVNFDGRVSFLEYLLYQYKDFANPADFCTRSMNHDEH 180 Query: 181 PEIKKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNMLAQIDSGPLKEQLNFA 240 PEIKKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNMLAQIDSGPLKEQLNFA Sbjct: 181 PEIKKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNMLAQIDSGPLKEQLNFA 240 Query: 241 LISAEAAVRTASKKYGGAAYSGGAGDAGAGSSAGAIWWMNRDLEEKKKRYGPQKK 295 LISAEAAVRTASKKYGGAAYSGGAGDAGAGSSAGAIWWMNRDLEEKKKRYGPQKK Sbjct: 241 LISAEAAVRTASKKYGGAAYSGGAGDAGAGSSAGAIWWMNRDLEEKKKRYGPQKK 295 >1QX2-A nrPDB Length = 76 Score = 32.0 bits (71), Expect = 0.29, Method: Compositional matrix adjust. Identities = 15/36 (41%), Positives = 21/36 (58%) Query: 127 PQMLTALKRKQELREKVDVNFDGRVSFLEYLLYQYK 162 P +L + E+ E+VD N DG VSF E+L+ K Sbjct: 38 PSLLKGMSTLDEMIEEVDKNGDGEVSFEEFLVMMKK 73 >1V1S-A nrPDB Length = 309 Score = 28.5 bits (62), Expect = 3.4, Method: Compositional matrix adjust. Identities = 34/112 (30%), Positives = 56/112 (50%), Gaps = 18/112 (16%) Query: 184 KKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNMLAQIDSGPLKEQLNFALIS 243 + R++L+ VN R + E+AR E +PGV L + A++ G ++E L AL + Sbjct: 156 RGVRVSLD-VNYRQTLWSPEEARGFLERALPGVDLLFLSEEEAELLFGRVEEALR-ALSA 213 Query: 244 AEAAVRTASK---------KYGGAAYS------GGAGDA-GAGSSAGAIWWM 279 E ++ +K + G+A++ GAGDA AG AGA+W + Sbjct: 214 PEVVLKRGAKGAWAFVDGRRVEGSAFAVEAVDPVGAGDAFAAGYLAGAVWGL 265 >1KQV-A nrPDB Length = 79 Score = 28.1 bits (61), Expect = 4.1, Method: Compositional matrix adjust. Identities = 13/30 (43%), Positives = 18/30 (60%) Query: 127 PQMLTALKRKQELREKVDVNFDGRVSFLEY 156 P +L + EL E++D N DG VSF E+ Sbjct: 41 PSLLKGMSTLDELFEELDKNGDGEVSFEEF 70 >1BOD-A nrPDB Length = 74 Score = 28.1 bits (61), Expect = 4.8, Method: Compositional matrix adjust. Identities = 13/30 (43%), Positives = 18/30 (60%) Query: 127 PQMLTALKRKQELREKVDVNFDGRVSFLEY 156 P +L + EL E++D N DG VSF E+ Sbjct: 36 PSLLKGMSTLDELFEELDKNGDGEVSFEEF 65 >1YS4-A nrPDB Length = 354 Score = 27.7 bits (60), Expect = 5.9, Method: Compositional matrix adjust. Identities = 21/70 (30%), Positives = 29/70 (41%), Gaps = 16/70 (22%) Query: 219 LGATNMLAQIDSGPLKEQLNFALISAEAAVRTASKKYGGAAYSGGAGDAGAGSSAGAIWW 278 LGAT + Q L + F L + A+ R+A KKY A Y W+ Sbjct: 14 LGATGSVGQRFVQLLADHPMFELTALAASERSAGKKYKDACY----------------WF 57 Query: 279 MNRDLEEKKK 288 +RD+ E K Sbjct: 58 QDRDIPENIK 67 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4X3N-A nrPDB 637 0.0 Sequences not found previously or not previously below threshold: 1V1S-A nrPDB 36 0.016 5XG2-A nrPDB 32 0.22 1QX2-A nrPDB 32 0.24 6F1U-X nrPDB 30 1.1 1BOD-A nrPDB 29 2.7 5XK7-A nrPDB 29 3.1 1KQV-A nrPDB 29 3.4 CONVERGED! >4X3N-A nrPDB Length = 295 Score = 637 bits (1644), Expect = 0.0, Method: Composition-based stats. Identities = 295/295 (100%), Positives = 295/295 (100%) Query: 1 MAETKVAPNLTGIEQTKAGQSFTEKLSAEAMEFFCNVAKLPFSQQAVHFLNAYWAEVSKE 60 MAETKVAPNLTGIEQTKAGQSFTEKLSAEAMEFFCNVAKLPFSQQAVHFLNAYWAEVSKE Sbjct: 1 MAETKVAPNLTGIEQTKAGQSFTEKLSAEAMEFFCNVAKLPFSQQAVHFLNAYWAEVSKE 60 Query: 61 AEFIYSVGWETIKYADMHCKGIQLVFKYDEGNDLDFDIALYFYEQLCKFCEDPKNKNYAT 120 AEFIYSVGWETIKYADMHCKGIQLVFKYDEGNDLDFDIALYFYEQLCKFCEDPKNKNYAT Sbjct: 61 AEFIYSVGWETIKYADMHCKGIQLVFKYDEGNDLDFDIALYFYEQLCKFCEDPKNKNYAT 120 Query: 121 TYPISQPQMLTALKRKQELREKVDVNFDGRVSFLEYLLYQYKDFANPADFCTRSMNHDEH 180 TYPISQPQMLTALKRKQELREKVDVNFDGRVSFLEYLLYQYKDFANPADFCTRSMNHDEH Sbjct: 121 TYPISQPQMLTALKRKQELREKVDVNFDGRVSFLEYLLYQYKDFANPADFCTRSMNHDEH 180 Query: 181 PEIKKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNMLAQIDSGPLKEQLNFA 240 PEIKKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNMLAQIDSGPLKEQLNFA Sbjct: 181 PEIKKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNMLAQIDSGPLKEQLNFA 240 Query: 241 LISAEAAVRTASKKYGGAAYSGGAGDAGAGSSAGAIWWMNRDLEEKKKRYGPQKK 295 LISAEAAVRTASKKYGGAAYSGGAGDAGAGSSAGAIWWMNRDLEEKKKRYGPQKK Sbjct: 241 LISAEAAVRTASKKYGGAAYSGGAGDAGAGSSAGAIWWMNRDLEEKKKRYGPQKK 295 >1V1S-A nrPDB Length = 309 Score = 36.2 bits (82), Expect = 0.016, Method: Composition-based stats. Identities = 35/115 (30%), Positives = 57/115 (49%), Gaps = 18/115 (15%) Query: 179 EHPEIKKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNMLAQIDSGPLKEQLN 238 E + + R++L+ VN R + E+AR E +PGV L + A++ G ++E L Sbjct: 151 EEAKRRGVRVSLD-VNYRQTLWSPEEARGFLERALPGVDLLFLSEEEAELLFGRVEEALR 209 Query: 239 FALISAEAAVRTASK---------KYGGAAYS------GGAGDA-GAGSSAGAIW 277 AL + E ++ +K + G+A++ GAGDA AG AGA+W Sbjct: 210 -ALSAPEVVLKRGAKGAWAFVDGRRVEGSAFAVEAVDPVGAGDAFAAGYLAGAVW 263 >5XG2-A nrPDB Length = 260 Score = 32.4 bits (72), Expect = 0.22, Method: Composition-based stats. Identities = 17/41 (41%), Positives = 27/41 (65%), Gaps = 5/41 (12%) Query: 182 EIKKARLALEEVNKRIRAYEEEKAR-----LTEESKIPGVK 217 E+++AR +L E RI+ EEEK R LT E+++PG++ Sbjct: 53 ELEEARKSLYEGEARIKRAEEEKERLKAEILTGEARLPGLR 93 >1QX2-A nrPDB Length = 76 Score = 32.4 bits (72), Expect = 0.24, Method: Composition-based stats. Identities = 15/36 (41%), Positives = 21/36 (58%) Query: 127 PQMLTALKRKQELREKVDVNFDGRVSFLEYLLYQYK 162 P +L + E+ E+VD N DG VSF E+L+ K Sbjct: 38 PSLLKGMSTLDEMIEEVDKNGDGEVSFEEFLVMMKK 73 >6F1U-X nrPDB Length = 577 Score = 30.1 bits (66), Expect = 1.1, Method: Composition-based stats. Identities = 28/124 (22%), Positives = 56/124 (45%), Gaps = 11/124 (8%) Query: 111 EDPKNKNYATTYPISQPQMLTA-----LKRKQELREKVDVNFDGRVSFLEYLLYQYKDFA 165 ED + KN +T I + + L A RK+EL ++ + L+ + + +D Sbjct: 235 EDFREKNSSTNQHIIRLESLQAEIKMLSDRKRELEHRLSATLEEN-DLLQGTVEELQDRV 293 Query: 166 NPADFCTRSMNHDEHPEIKKARLALEEVNKRIRAYEEEKARLTEESKIPGVKGLGATNML 225 HD+ ++ +++L L+EV R + + LTEE + +T++L Sbjct: 294 ----LILERQGHDKDLQLHQSQLELQEVRLSYRQLQGKVEELTEERSLQS-SAATSTSLL 348 Query: 226 AQID 229 ++I+ Sbjct: 349 SEIE 352 >1BOD-A nrPDB Length = 74 Score = 28.9 bits (63), Expect = 2.7, Method: Composition-based stats. Identities = 13/30 (43%), Positives = 18/30 (60%) Query: 127 PQMLTALKRKQELREKVDVNFDGRVSFLEY 156 P +L + EL E++D N DG VSF E+ Sbjct: 36 PSLLKGMSTLDELFEELDKNGDGEVSFEEF 65 >5XK7-A nrPDB Length = 232 Score = 28.9 bits (63), Expect = 3.1, Method: Composition-based stats. Identities = 26/111 (23%), Positives = 44/111 (39%), Gaps = 7/111 (6%) Query: 44 QQAVHFLNAYWAEVSKEAEFIYSVGWETIKYADMHCKGIQLVFKYDEGNDLDFDIALYFY 103 +Q + ++Y A K EF GW + + V Y + A+ + Sbjct: 32 KQGISLDDSYAAMTDKLVEF---TGWAREEGFTTFYVTVSSVANYSRSEE-QVTTAMNAF 87 Query: 104 EQLCKFCEDPKNKNYATTYPISQPQMLTALKRKQELREKVDVNFDGRVSFL 154 ++ + C D N NY+ T + + LT L + LR K D D + F+ Sbjct: 88 TEVVRRCHDTLNFNYSGTLEVVPERWLTEL---EALRAKSDSQSDFTLHFI 135 >1KQV-A nrPDB Length = 79 Score = 28.5 bits (62), Expect = 3.4, Method: Composition-based stats. Identities = 13/30 (43%), Positives = 18/30 (60%) Query: 127 PQMLTALKRKQELREKVDVNFDGRVSFLEY 156 P +L + EL E++D N DG VSF E+ Sbjct: 41 PSLLKGMSTLDELFEELDKNGDGEVSFEEF 70 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.132 0.386 Lambda K H 0.267 0.0411 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,629,567 Number of Sequences: 36641 Number of extensions: 466104 Number of successful extensions: 1594 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 1558 Number of HSP's gapped (non-prelim): 50 length of query: 295 length of database: 10,336,785 effective HSP length: 96 effective length of query: 199 effective length of database: 6,819,249 effective search space: 1357030551 effective search space used: 1357030551 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 59 (27.3 bits)