BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P23968 (213 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5VOZ-R nrPDB 417 e-117 6O7T-g nrPDB 78 3e-15 6O7U-o nrPDB 69 2e-12 5TJ5-D nrPDB 66 1e-11 5AA5-C nrPDB 28 3.1 6FKH-S nrPDB 28 3.6 3M9I-A nrPDB 27 5.7 1YMG-A nrPDB 27 7.1 2XQS-A nrPDB 27 7.6 >5VOZ-R nrPDB Length = 213 Score = 417 bits (1073), Expect = e-117, Method: Compositional matrix adjust. Identities = 213/213 (100%), Positives = 213/213 (100%) Query: 1 MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSDINFGKFLLRTSPYMWA 60 MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSDINFGKFLLRTSPYMWA Sbjct: 1 MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSDINFGKFLLRTSPYMWA 60 Query: 61 NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIV 120 NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIV Sbjct: 61 NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIV 120 Query: 121 FSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSAL 180 FSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSAL Sbjct: 121 FSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSAL 180 Query: 181 FVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ 213 FVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ Sbjct: 181 FVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ 213 >6O7T-g nrPDB Length = 160 Score = 77.8 bits (190), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 52/157 (33%), Positives = 85/157 (54%), Gaps = 9/157 (5%) Query: 55 SPYMWANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYG 114 +P+ A +G A + + +GAA+G +G + V P + KN++ +I ++AIYG Sbjct: 9 APFFGA-IGCASAIIFTSLGAAYGTAKSGVGICATCVLRPDLLFKNIVPVIMAGIIAIYG 67 Query: 115 LIIAIVFSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISD 174 L+++++ L K LYTG+ AG++VG S L G A+GI G Sbjct: 68 LVVSVLVCYSLG--------QKQALYTGFIQLGAGLSVGLSGLAAGFAIGIVGDAGVRGS 119 Query: 175 AADSALFVKILVIEIFGSILGLLGLIVGLLMAGKASE 211 + LFV +++I IF +LGL GLIV LL+ +A++ Sbjct: 120 SQQPRLFVGMILILIFAEVLGLYGLIVALLLNSRATQ 156 >6O7U-o nrPDB Length = 164 Score = 68.9 bits (167), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 48/149 (32%), Positives = 77/149 (51%), Gaps = 6/149 (4%) Query: 63 GIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIVFS 122 G A + LS +GAA G +G + G G P + K+LI ++ ++AIYGL++A++ + Sbjct: 22 GCAAAMVLSCLGAAIGTAKSGIGIAGIGTFKPELIMKSLIPVVMSGILAIYGLVVAVLIA 81 Query: 123 SKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSALFV 182 L+ L+ G+ G+ VG + L G A+G+ G LFV Sbjct: 82 GNLSPT------EDYTLFNGFMHLSCGLCVGFACLSSGYAIGMVGDVGVRKYMHQPRLFV 135 Query: 183 KILVIEIFGSILGLLGLIVGLLMAGKASE 211 I++I IF +LGL G+IV L++ + SE Sbjct: 136 GIVLILIFSEVLGLYGMIVALILNTRGSE 164 Score = 37.7 bits (86), Expect = 0.003, Method: Compositional matrix adjust. Identities = 17/66 (25%), Positives = 39/66 (59%) Query: 59 WANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIA 118 + +L LCVG + + + + I + G + + PR+ ++ +IF EV+ +YG+I+A Sbjct: 96 FMHLSCGLCVGFACLSSGYAIGMVGDVGVRKYMHQPRLFVGIVLILIFSEVLGLYGMIVA 155 Query: 119 IVFSSK 124 ++ +++ Sbjct: 156 LILNTR 161 >5TJ5-D nrPDB Length = 147 Score = 65.9 bits (159), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 45/141 (31%), Positives = 73/141 (51%), Gaps = 6/141 (4%) Query: 70 LSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIVFSSKLTVAT 129 LS +GAA G +G + G G P + K+LI ++ ++AIYGL++A++ + L+ Sbjct: 13 LSCLGAAIGTAKSGIGIAGIGTFKPELIMKSLIPVVMSGILAIYGLVVAVLIAGNLSPT- 71 Query: 130 AENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSALFVKILVIEI 189 L+ G+ G+ VG + L G A+G+ G LFV I++I I Sbjct: 72 -----EDYTLFNGFMHLSCGLCVGFACLSSGYAIGMVGDVGVRKYMHQPRLFVGIVLILI 126 Query: 190 FGSILGLLGLIVGLLMAGKAS 210 F +LGL G+IV L++ + S Sbjct: 127 FSEVLGLYGMIVALILNTRGS 147 Score = 37.7 bits (86), Expect = 0.004, Method: Compositional matrix adjust. Identities = 17/66 (25%), Positives = 39/66 (59%) Query: 59 WANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIA 118 + +L LCVG + + + + I + G + + PR+ ++ +IF EV+ +YG+I+A Sbjct: 80 FMHLSCGLCVGFACLSSGYAIGMVGDVGVRKYMHQPRLFVGIVLILIFSEVLGLYGMIVA 139 Query: 119 IVFSSK 124 ++ +++ Sbjct: 140 LILNTR 145 >5AA5-C nrPDB Length = 579 Score = 28.1 bits (61), Expect = 3.1, Method: Compositional matrix adjust. Identities = 18/64 (28%), Positives = 27/64 (42%), Gaps = 4/64 (6%) Query: 108 EVVAIYGLIIAIVFSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITG 167 +V G+ I F ++ YS S+++ GYS+F G S+ I GI G Sbjct: 30 RIVGSLGIYTKIDFENRRVA----ECYSTSSIFRGYSIFMKGKDPRDSHFITSRICGICG 85 Query: 168 ATAA 171 A Sbjct: 86 DNHA 89 >6FKH-S nrPDB Length = 81 Score = 27.7 bits (60), Expect = 3.6, Method: Compositional matrix adjust. Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 4/60 (6%) Query: 66 LCVGLSVVGAAWGIFITGSSMIGAGVRAP----RITTKNLISIIFCEVVAIYGLIIAIVF 121 L VGL+ +G G + R P +I L+S+ F E + IYGL++A+ Sbjct: 15 LAVGLASIGPGVGQGTAAGQAVEGIARQPEAEGKIRGTLLLSLAFMEALTIYGLVVALAL 74 >3M9I-A nrPDB Length = 220 Score = 26.9 bits (58), Expect = 5.7, Method: Compositional matrix adjust. Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 1/41 (2%) Query: 64 IALCVGLSV-VGAAWGIFITGSSMIGAGVRAPRITTKNLIS 103 +AL VG S+ +G +G++ TG+ M A AP I T+N + Sbjct: 154 VALAVGFSLTLGHLFGMYYTGAGMNPARSFAPAILTRNFTN 194 >1YMG-A nrPDB Length = 263 Score = 26.9 bits (58), Expect = 7.1, Method: Compositional matrix adjust. Identities = 16/38 (42%), Positives = 24/38 (63%), Gaps = 1/38 (2%) Query: 64 IALCVGLSV-VGAAWGIFITGSSMIGAGVRAPRITTKN 100 +AL VG S+ +G +G++ TG+ M A AP I T+N Sbjct: 160 VALAVGFSLTLGHLFGMYYTGAGMNPARSFAPAILTRN 197 >2XQS-A nrPDB Length = 82 Score = 26.6 bits (57), Expect = 7.6, Method: Compositional matrix adjust. Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 4/58 (6%) Query: 68 VGLSVVGAAWGIFITGSSMIGAGVRAP----RITTKNLISIIFCEVVAIYGLIIAIVF 121 VG+ +G G + R P +I L+S+ F E + IYGL++A+V Sbjct: 18 VGIGSIGPGLGQGQAAGQAVEGIARQPEAEGKIRGTLLLSLAFMEALTIYGLVVALVL 75 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5VOZ-R nrPDB 273 3e-74 6O7U-o nrPDB 170 4e-43 6O7T-g nrPDB 165 8e-42 5TJ5-D nrPDB 163 5e-41 Sequences not found previously or not previously below threshold: 2BL2-A nrPDB 51 5e-07 5Y5Z-O nrPDB 36 0.013 6FKH-S nrPDB 29 1.7 4BEM-J nrPDB 28 3.5 4BEM-A nrPDB 27 6.7 3OH9-A nrPDB 26 8.6 >5VOZ-R nrPDB Length = 213 Score = 273 bits (699), Expect = 3e-74, Method: Composition-based stats. Identities = 213/213 (100%), Positives = 213/213 (100%) Query: 1 MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSDINFGKFLLRTSPYMWA 60 MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSDINFGKFLLRTSPYMWA Sbjct: 1 MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSDINFGKFLLRTSPYMWA 60 Query: 61 NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIV 120 NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIV Sbjct: 61 NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIV 120 Query: 121 FSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSAL 180 FSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSAL Sbjct: 121 FSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSAL 180 Query: 181 FVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ 213 FVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ Sbjct: 181 FVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ 213 >6O7U-o nrPDB Length = 164 Score = 170 bits (431), Expect = 4e-43, Method: Composition-based stats. Identities = 49/157 (31%), Positives = 80/157 (50%), Gaps = 7/157 (4%) Query: 55 SPYMWANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYG 114 +P+ G A + LS +GAA G +G + G G P + K+LI ++ ++AIYG Sbjct: 15 APFFG-FAGCAAAMVLSCLGAAIGTAKSGIGIAGIGTFKPELIMKSLIPVVMSGILAIYG 73 Query: 115 LIIAIVFSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISD 174 L++A++ + L+ L+ G+ G+ VG + L G A+G+ G Sbjct: 74 LVVAVLIAGNLSPT------EDYTLFNGFMHLSCGLCVGFACLSSGYAIGMVGDVGVRKY 127 Query: 175 AADSALFVKILVIEIFGSILGLLGLIVGLLMAGKASE 211 LFV I++I IF +LGL G+IV L++ + SE Sbjct: 128 MHQPRLFVGIVLILIFSEVLGLYGMIVALILNTRGSE 164 >6O7T-g nrPDB Length = 160 Score = 165 bits (419), Expect = 8e-42, Method: Composition-based stats. Identities = 52/157 (33%), Positives = 85/157 (54%), Gaps = 9/157 (5%) Query: 55 SPYMWANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYG 114 +P+ A +G A + + +GAA+G +G + V P + KN++ +I ++AIYG Sbjct: 9 APFFGA-IGCASAIIFTSLGAAYGTAKSGVGICATCVLRPDLLFKNIVPVIMAGIIAIYG 67 Query: 115 LIIAIVFSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISD 174 L+++++ L K LYTG+ AG++VG S L G A+GI G Sbjct: 68 LVVSVLVCYSLG--------QKQALYTGFIQLGAGLSVGLSGLAAGFAIGIVGDAGVRGS 119 Query: 175 AADSALFVKILVIEIFGSILGLLGLIVGLLMAGKASE 211 + LFV +++I IF +LGL GLIV LL+ +A++ Sbjct: 120 SQQPRLFVGMILILIFAEVLGLYGLIVALLLNSRATQ 156 >5TJ5-D nrPDB Length = 147 Score = 163 bits (413), Expect = 5e-41, Method: Composition-based stats. Identities = 47/153 (30%), Positives = 77/153 (50%), Gaps = 6/153 (3%) Query: 58 MWANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLII 117 + G A + LS +GAA G +G + G G P + K+LI ++ ++AIYGL++ Sbjct: 1 FFGFAGCAAAMVLSCLGAAIGTAKSGIGIAGIGTFKPELIMKSLIPVVMSGILAIYGLVV 60 Query: 118 AIVFSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAAD 177 A++ + L+ L+ G+ G+ VG + L G A+G+ G Sbjct: 61 AVLIAGNLSPT------EDYTLFNGFMHLSCGLCVGFACLSSGYAIGMVGDVGVRKYMHQ 114 Query: 178 SALFVKILVIEIFGSILGLLGLIVGLLMAGKAS 210 LFV I++I IF +LGL G+IV L++ + S Sbjct: 115 PRLFVGIVLILIFSEVLGLYGMIVALILNTRGS 147 >2BL2-A nrPDB Length = 156 Score = 50.7 bits (120), Expect = 5e-07, Method: Composition-based stats. Identities = 31/146 (21%), Positives = 54/146 (36%), Gaps = 8/146 (5%) Query: 60 ANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAI 119 A L +A S +G+A G+ +TG + P + LI + +YG +IA Sbjct: 15 AVLAMATATIFSGIGSAKGVGMTGEAAAALTTSQPEKFGQALILQLLPGTQGLYGFVIAF 74 Query: 120 VFSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSA 179 + L S ++ G + A + + + L GIA G A A Sbjct: 75 LIFINLG--------SDMSVVQGLNFLGASLPIAFTGLFSGIAQGKVAAAGIQILAKKPE 126 Query: 180 LFVKILVIEIFGSILGLLGLIVGLLM 205 K ++ +LG ++ L+ Sbjct: 127 HATKGIIFAAMVETYAILGFVISFLL 152 Score = 29.5 bits (65), Expect = 0.96, Method: Composition-based stats. Identities = 13/63 (20%), Positives = 27/63 (42%) Query: 143 YSLFWAGITVGASNLICGIAVGITGATAAISDAADSALFVKILVIEIFGSILGLLGLIVG 202 +++ S + VG+TG AA + F + L++++ GL G ++ Sbjct: 14 FAVLAMATATIFSGIGSAKGVGMTGEAAAALTTSQPEKFGQALILQLLPGTQGLYGFVIA 73 Query: 203 LLM 205 L+ Sbjct: 74 FLI 76 >5Y5Z-O nrPDB Length = 99 Score = 36.1 bits (82), Expect = 0.013, Method: Composition-based stats. Identities = 21/94 (22%), Positives = 38/94 (40%) Query: 115 LIIAIVFSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISD 174 L+ ++ AE + L G G+ VG + L G+A GA + Sbjct: 5 LVTVLLAVFGALAFAAEEAAASGGLDRGLIAVGMGLAVGLAALGTGVAQARIGAAGVGAI 64 Query: 175 AADSALFVKILVIEIFGSILGLLGLIVGLLMAGK 208 A D + F L+ + L + GL++ ++ G+ Sbjct: 65 AEDRSNFGTALIFLLLPETLVIFGLLIAFILNGR 98 >6FKH-S nrPDB Length = 81 Score = 28.8 bits (63), Expect = 1.7, Method: Composition-based stats. Identities = 19/71 (26%), Positives = 29/71 (40%), Gaps = 4/71 (5%) Query: 139 LYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSALFVKI----LVIEIFGSIL 194 L S+ AG+ VG +++ G+ G A A KI L+ F L Sbjct: 4 LIAAASVIAAGLAVGLASIGPGVGQGTAAGQAVEGIARQPEAEGKIRGTLLLSLAFMEAL 63 Query: 195 GLLGLIVGLLM 205 + GL+V L + Sbjct: 64 TIYGLVVALAL 74 >4BEM-J nrPDB Length = 182 Score = 28.0 bits (61), Expect = 3.5, Method: Composition-based stats. Identities = 32/152 (21%), Positives = 60/152 (39%), Gaps = 10/152 (6%) Query: 60 ANLGIALCVGLSV---VGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLI 116 + LGI L + V +G + ++ ++ I L+ E I+ L+ Sbjct: 28 SALGIGLAMVAGVGPGIGQGFAAGKGAEAVGKNPTKSNDIVMIMLLGAAVAETSGIFSLV 87 Query: 117 IAIVFSSK---LTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAIS 173 IA++ ++ + + S S + +G ++ AGI G G A G I Sbjct: 88 IALILLFANPFISSTASVWILSASAMASGIAMI-AGIGPGTG---QGYAAGKGAEAVGIR 143 Query: 174 DAADSALFVKILVIEIFGSILGLLGLIVGLLM 205 SA+ +L+ + G+ LIV L++ Sbjct: 144 PEMKSAILRVMLLGQAVAQTTGIYALIVALIL 175 >4BEM-A nrPDB Length = 82 Score = 26.8 bits (58), Expect = 6.7, Method: Composition-based stats. Identities = 12/50 (24%), Positives = 19/50 (38%) Query: 156 NLICGIAVGITGATAAISDAADSALFVKILVIEIFGSILGLLGLIVGLLM 205 + G A G A S + +L+ G+ GLIV L++ Sbjct: 26 GIGQGFAAGKGAEAVGRQPEAQSDIIRTMLLGAAVAETTGIYGLIVALIL 75 >3OH9-A nrPDB Length = 289 Score = 26.5 bits (57), Expect = 8.6, Method: Composition-based stats. Identities = 15/76 (19%), Positives = 32/76 (42%) Query: 90 GVRAPRITTKNLISIIFCEVVAIYGLIIAIVFSSKLTVATAENMYSKSNLYTGYSLFWAG 149 G P + + V AI G ++++ ++KLT A+ + + + Y F Sbjct: 106 GAARPGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTP 165 Query: 150 ITVGASNLICGIAVGI 165 + A++ A+G+ Sbjct: 166 QRLAAADPQALKALGM 181 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.327 0.144 0.394 Lambda K H 0.267 0.0454 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,354,856 Number of Sequences: 36641 Number of extensions: 334776 Number of successful extensions: 1027 Number of sequences better than 10.0: 40 Number of HSP's better than 10.0 without gapping: 27 Number of HSP's successfully gapped in prelim test: 32 Number of HSP's that attempted gapping in prelim test: 954 Number of HSP's gapped (non-prelim): 79 length of query: 213 length of database: 10,336,785 effective HSP length: 93 effective length of query: 120 effective length of database: 6,929,172 effective search space: 831500640 effective search space used: 831500640 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 57 (26.4 bits)