BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P22943 (109 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2LJL-A nrPDB 217 1e-57 3K8P-D nrPDB 26 4.0 4GDF-A nrPDB 26 4.6 2RHQ-B nrPDB 26 5.1 1SVL-A nrPDB 25 6.8 1E3D-B nrPDB 25 8.4 >2LJL-A nrPDB Length = 109 Score = 217 bits (552), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 109/109 (100%), Positives = 109/109 (100%) Query: 1 MSDAGRKGFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSA 60 MSDAGRKGFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSA Sbjct: 1 MSDAGRKGFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSA 60 Query: 61 EKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGEEDPTKK 109 EKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGEEDPTKK Sbjct: 61 EKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGEEDPTKK 109 >3K8P-D nrPDB Length = 709 Score = 25.8 bits (55), Expect = 4.0, Method: Composition-based stats. Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 3/66 (4%) Query: 5 GRKGFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSAEKG- 63 G G + + L P SY ++ + +IT+ +K ++ + N +F ++ S EKG Sbjct: 206 GYIGIADVIEDELAP--TLSYGKKWETFITEFFNKQQFSLKSDTNYQLFIKLYYSLEKGV 263 Query: 64 KDNAEG 69 KDN+E Sbjct: 264 KDNSEA 269 >4GDF-A nrPDB Length = 497 Score = 25.8 bits (55), Expect = 4.6, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Query: 52 VFQGVHDSAEKGKDNAEGQGESLADQARDYM-GAAKSKL 89 VF+ V + + +D GQG + D RDY+ G+ K L Sbjct: 341 VFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNL 379 >2RHQ-B nrPDB Length = 795 Score = 25.8 bits (55), Expect = 5.1, Method: Compositional matrix adjust. Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 6/47 (12%) Query: 57 HDSAEKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGE 103 ++ A K KD L + R + G + +L D VEY+SG + GE Sbjct: 563 YNVARKNKD------VRLYEIGRVFFGNGEGELPDEVEYLSGILTGE 603 >1SVL-A nrPDB Length = 377 Score = 25.0 bits (53), Expect = 6.8, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Query: 52 VFQGVHDSAEKGKDNAEGQGESLADQARDYM-GAAKSKL 89 VF+ V + + +D GQG + D RDY+ G+ K L Sbjct: 221 VFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNL 259 >1E3D-B nrPDB Length = 542 Score = 25.0 bits (53), Expect = 8.4, Method: Composition-based stats. Identities = 19/66 (28%), Positives = 29/66 (43%), Gaps = 5/66 (7%) Query: 8 GFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSAEKGKDNA 67 FGE + + D + + G Y D+ G VQP D + + V S +GK A Sbjct: 288 AFGEFPAPGGERDLNSRWYKPGVIY-----DRKVGSVQPFDPSKIEEHVRHSWYEGKARA 342 Query: 68 EGQGES 73 +GE+ Sbjct: 343 PFEGET 348 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2LJL-A nrPDB 166 2e-42 Sequences not found previously or not previously below threshold: 3DO6-A nrPDB 29 0.39 5EMU-A nrPDB 28 0.77 6FRK-r nrPDB 28 0.81 6CY8-B nrPDB 28 0.89 1E3D-B nrPDB 28 1.2 2RHQ-B nrPDB 28 1.2 4GDF-A nrPDB 27 1.5 1SVL-A nrPDB 27 1.5 3MAN-A nrPDB 27 2.0 CONVERGED! >2LJL-A nrPDB Length = 109 Score = 166 bits (421), Expect = 2e-42, Method: Composition-based stats. Identities = 109/109 (100%), Positives = 109/109 (100%) Query: 1 MSDAGRKGFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSA 60 MSDAGRKGFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSA Sbjct: 1 MSDAGRKGFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSA 60 Query: 61 EKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGEEDPTKK 109 EKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGEEDPTKK Sbjct: 61 EKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGEEDPTKK 109 >3DO6-A nrPDB Length = 543 Score = 29.4 bits (64), Expect = 0.39, Method: Composition-based stats. Identities = 21/79 (26%), Positives = 37/79 (46%), Gaps = 10/79 (12%) Query: 32 YITDKADKVAGKVQPEDNKGVFQGVHDSAEKGKDNAEGQGESLADQARDYMGAAKSKLND 91 + TD ++A V+ + GV V + +KG + +++A+ A+D A ++ND Sbjct: 370 FSTDTEKEIAYVVKECEKLGVRVAVSEVFKKGSEGGVELAKAVAEAAKDVEPAYLYEMND 429 Query: 92 AVE----------YVSGRV 100 VE Y +GRV Sbjct: 430 PVEKKIEILAKEIYRAGRV 448 >5EMU-A nrPDB Length = 265 Score = 28.2 bits (61), Expect = 0.77, Method: Composition-based stats. Identities = 20/77 (25%), Positives = 32/77 (41%), Gaps = 1/77 (1%) Query: 10 GEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHD-SAEKGKDNAE 68 GE EAL + + + G ++I KVA PE + + + + D EK Sbjct: 143 GELKDEALIRKASEISIKAGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKP 202 Query: 69 GQGESLADQARDYMGAA 85 G A+ A+ Y+ A Sbjct: 203 AGGVRTAEDAQKYLAIA 219 >6FRK-r nrPDB Length = 605 Score = 28.2 bits (61), Expect = 0.81, Method: Composition-based stats. Identities = 14/35 (40%), Positives = 18/35 (51%) Query: 14 SEALKPDSQKSYAEQGKEYITDKADKVAGKVQPED 48 S +LK D + G YI K KVAG QP++ Sbjct: 511 SMSLKVDVEALENSPGATYIRKKGGKVAGDSQPKE 545 >6CY8-B nrPDB Length = 380 Score = 28.2 bits (61), Expect = 0.89, Method: Composition-based stats. Identities = 18/63 (28%), Positives = 29/63 (46%), Gaps = 12/63 (19%) Query: 54 QGVHDSAEKGKDNA---EGQGESLADQARD---------YMGAAKSKLNDAVEYVSGRVH 101 Q D+ KD A EGQG + + D ++GA + LN +EY + R+ Sbjct: 211 QVFFDNCTIHKDYALGREGQGARIFAASMDWERCCLFAIFVGAMQRDLNQCIEYANTRMQ 270 Query: 102 GEE 104 G++ Sbjct: 271 GDK 273 >1E3D-B nrPDB Length = 542 Score = 27.8 bits (60), Expect = 1.2, Method: Composition-based stats. Identities = 19/66 (28%), Positives = 29/66 (43%), Gaps = 5/66 (7%) Query: 8 GFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSAEKGKDNA 67 FGE + + D + + G Y D+ G VQP D + + V S +GK A Sbjct: 288 AFGEFPAPGGERDLNSRWYKPGVIY-----DRKVGSVQPFDPSKIEEHVRHSWYEGKARA 342 Query: 68 EGQGES 73 +GE+ Sbjct: 343 PFEGET 348 >2RHQ-B nrPDB Length = 795 Score = 27.8 bits (60), Expect = 1.2, Method: Composition-based stats. Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 6/47 (12%) Query: 57 HDSAEKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGE 103 ++ A K KD L + R + G + +L D VEY+SG + GE Sbjct: 563 YNVARKNKD------VRLYEIGRVFFGNGEGELPDEVEYLSGILTGE 603 >4GDF-A nrPDB Length = 497 Score = 27.4 bits (59), Expect = 1.5, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Query: 52 VFQGVHDSAEKGKDNAEGQGESLADQARDYM-GAAKSKL 89 VF+ V + + +D GQG + D RDY+ G+ K L Sbjct: 341 VFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNL 379 >1SVL-A nrPDB Length = 377 Score = 27.4 bits (59), Expect = 1.5, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Query: 52 VFQGVHDSAEKGKDNAEGQGESLADQARDYM-GAAKSKL 89 VF+ V + + +D GQG + D RDY+ G+ K L Sbjct: 221 VFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNL 259 >3MAN-A nrPDB Length = 302 Score = 27.1 bits (58), Expect = 2.0, Method: Composition-based stats. Identities = 17/41 (41%), Positives = 19/41 (46%), Gaps = 4/41 (9%) Query: 56 VHDSAEKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYV 96 VHD+ G E G S DQA DY KS L +YV Sbjct: 85 VHDTTGYG----EQSGASTLDQAVDYWIELKSVLQGEEDYV 121 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.302 0.124 0.335 Lambda K H 0.267 0.0394 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,472,708 Number of Sequences: 36641 Number of extensions: 167542 Number of successful extensions: 208 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 17 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 191 Number of HSP's gapped (non-prelim): 27 length of query: 109 length of database: 10,336,785 effective HSP length: 75 effective length of query: 34 effective length of database: 7,588,710 effective search space: 258016140 effective search space used: 258016140 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (21.7 bits) S2: 52 (24.7 bits)