BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P22289 (66 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6HU9-I nrPDB 134 8e-33 4D6T-J nrPDB 48 1e-06 3H1L-J nrPDB 45 5e-06 5XTI-AD nrPDB 45 6e-06 2VE3-A nrPDB 25 6.2 4AXG-A nrPDB 25 9.4 >6HU9-I nrPDB Length = 66 Score = 134 bits (338), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 66/66 (100%), Positives = 66/66 (100%) Query: 1 MSFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD 60 MSFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD Sbjct: 1 MSFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD 60 Query: 61 GDDDDE 66 GDDDDE Sbjct: 61 GDDDDE 66 >4D6T-J nrPDB Length = 64 Score = 47.8 bits (112), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 21/52 (40%), Positives = 30/52 (57%) Query: 4 SSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKAR 55 + LY F+R + F TI GA F+ FD + YE+ N+GKLWK +K + Sbjct: 8 ARLYSLLFRRTSTFALTIVVGALFFERAFDQGADAIYEHINEGKLWKHIKHK 59 >3H1L-J nrPDB Length = 61 Score = 45.4 bits (106), Expect = 5e-06, Method: Compositional matrix adjust. Identities = 19/54 (35%), Positives = 31/54 (57%) Query: 7 YKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD 60 Y F+R + F T+ GA +F+ FD + +E+ N+GKLWK +K + A + Sbjct: 7 YSALFRRTSTFALTVVLGAVLFERAFDQGADAIFEHLNEGKLWKHIKHKYEASE 60 >5XTI-AD nrPDB Length = 62 Score = 45.4 bits (106), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 20/52 (38%), Positives = 29/52 (55%) Query: 4 SSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKAR 55 S LY F+R + F TI G F+ FD + Y++ N+GKLWK +K + Sbjct: 7 SKLYSLLFRRTSTFALTIIVGVMFFERAFDQGADAIYDHINEGKLWKHIKHK 58 >2VE3-A nrPDB Length = 444 Score = 25.4 bits (54), Expect = 6.2, Method: Composition-based stats. Identities = 11/38 (28%), Positives = 19/38 (50%), Gaps = 1/38 (2%) Query: 3 FSSLYKT-FFKRNAVFVGTIFAGAFVFQTVFDTAITSW 39 F ++KT F +N +F+ A F+F +T +W Sbjct: 43 FGPIFKTRLFGKNVIFISGALANRFLFTKEQETFQATW 80 >4AXG-A nrPDB Length = 248 Score = 24.6 bits (52), Expect = 9.4, Method: Composition-based stats. Identities = 9/28 (32%), Positives = 17/28 (60%) Query: 37 TSWYENHNKGKLWKDVKARIAAGDGDDD 64 T WY +++ K W+D++ I + D +D Sbjct: 77 TLWYLENDRSKSWEDMQNEITSFDTVED 104 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6HU9-I nrPDB 98 1e-21 4D6T-J nrPDB 94 2e-20 5XTI-AD nrPDB 89 4e-19 3H1L-J nrPDB 87 1e-18 Sequences not found previously or not previously below threshold: 4YNU-A nrPDB 29 0.42 3RBM-A nrPDB 26 4.7 3WE9-A nrPDB 26 5.2 2CFM-A nrPDB 25 8.1 6GAW-Ah nrPDB 25 9.6 2XE4-A nrPDB 25 9.7 CONVERGED! >6HU9-I nrPDB Length = 66 Score = 97.8 bits (242), Expect = 1e-21, Method: Composition-based stats. Identities = 66/66 (100%), Positives = 66/66 (100%) Query: 1 MSFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD 60 MSFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD Sbjct: 1 MSFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD 60 Query: 61 GDDDDE 66 GDDDDE Sbjct: 61 GDDDDE 66 >4D6T-J nrPDB Length = 64 Score = 93.6 bits (231), Expect = 2e-20, Method: Composition-based stats. Identities = 21/57 (36%), Positives = 31/57 (54%) Query: 4 SSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD 60 + LY F+R + F TI GA F+ FD + YE+ N+GKLWK +K + + Sbjct: 8 ARLYSLLFRRTSTFALTIVVGALFFERAFDQGADAIYEHINEGKLWKHIKHKYENKE 64 >5XTI-AD nrPDB Length = 62 Score = 88.9 bits (219), Expect = 4e-19, Method: Composition-based stats. Identities = 20/54 (37%), Positives = 29/54 (53%) Query: 4 SSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIA 57 S LY F+R + F TI G F+ FD + Y++ N+GKLWK +K + Sbjct: 7 SKLYSLLFRRTSTFALTIIVGVMFFERAFDQGADAIYDHINEGKLWKHIKHKYE 60 >3H1L-J nrPDB Length = 61 Score = 87.4 bits (215), Expect = 1e-18, Method: Composition-based stats. Identities = 19/54 (35%), Positives = 31/54 (57%) Query: 7 YKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD 60 Y F+R + F T+ GA +F+ FD + +E+ N+GKLWK +K + A + Sbjct: 7 YSALFRRTSTFALTVVLGAVLFERAFDQGADAIFEHLNEGKLWKHIKHKYEASE 60 >4YNU-A nrPDB Length = 571 Score = 29.2 bits (64), Expect = 0.42, Method: Composition-based stats. Identities = 11/59 (18%), Positives = 20/59 (33%) Query: 2 SFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGD 60 S++ + + GT + D I W + N+G WKD+ + Sbjct: 71 SYAGGKQQVLRAGKALGGTSTINGMAYTRAEDVQIDVWQKLGNEGWTWKDLLPYYLKSE 129 >3RBM-A nrPDB Length = 396 Score = 25.8 bits (55), Expect = 4.7, Method: Composition-based stats. Identities = 7/34 (20%), Positives = 18/34 (52%) Query: 24 GAFVFQTVFDTAITSWYENHNKGKLWKDVKARIA 57 G F+ ++D ++ + N+ L +++K I+ Sbjct: 33 GLAFFRNMYDKYRDAFLSHLNEYSLEEEIKEHIS 66 >3WE9-A nrPDB Length = 274 Score = 25.8 bits (55), Expect = 5.2, Method: Composition-based stats. Identities = 8/28 (28%), Positives = 10/28 (35%), Gaps = 2/28 (7%) Query: 31 VFDTAITSWYENHNKGK--LWKDVKARI 56 F+ Y+ N GK LWK Sbjct: 64 AFEAKADHVYQRTNNGKQHLWKAFDHAY 91 >2CFM-A nrPDB Length = 561 Score = 25.0 bits (53), Expect = 8.1, Method: Composition-based stats. Identities = 7/39 (17%), Positives = 15/39 (38%), Gaps = 8/39 (20%) Query: 27 VFQTVFDTA--------ITSWYENHNKGKLWKDVKARIA 57 ++ + + + YE N+GK W +K + Sbjct: 388 FYKRALEMGHEGLMAKRLDAVYEPGNRGKKWLKIKPTME 426 >6GAW-Ah nrPDB Length = 387 Score = 24.6 bits (52), Expect = 9.6, Method: Composition-based stats. Identities = 12/42 (28%), Positives = 16/42 (38%), Gaps = 1/42 (2%) Query: 25 AFVFQTVFDTAITSWYENHNKGKLWKDVKARIAAGDGDDDDE 66 A V + F + +GKLW+ AG DD E Sbjct: 279 AAVTERPFQNGFEEMIQWTKEGKLWE-FPINNEAGFDDDGSE 319 >2XE4-A nrPDB Length = 751 Score = 24.6 bits (52), Expect = 9.7, Method: Composition-based stats. Identities = 16/67 (23%), Positives = 28/67 (41%), Gaps = 7/67 (10%) Query: 1 MSFSSLYKTFFKRNAVFVGTIF--AGAFVFQTVFDTAITSWY-----ENHNKGKLWKDVK 53 MS + +Y FKR + TI + V+ TS + E + K+W+ V Sbjct: 195 MSGNEVYTIEFKRISDPSQTIADKVSGTNGEIVWGPDHTSLFYVTKDETLRENKVWRHVM 254 Query: 54 ARIAAGD 60 ++ + D Sbjct: 255 GKLQSED 261 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.137 0.397 Lambda K H 0.267 0.0430 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,924,597 Number of Sequences: 36641 Number of extensions: 94895 Number of successful extensions: 224 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 209 Number of HSP's gapped (non-prelim): 21 length of query: 66 length of database: 10,336,785 effective HSP length: 37 effective length of query: 29 effective length of database: 8,981,068 effective search space: 260450972 effective search space used: 260450972 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)