BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P22139 (70 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1WCM-J nrPDB 145 5e-36 6A5U-J nrPDB 115 5e-27 3H0G-J nrPDB 112 4e-26 5IYC-J nrPDB 106 3e-24 2PMZ-N nrPDB 77 1e-15 2WAQ-N nrPDB 75 6e-15 4QIW-N nrPDB 70 2e-13 4LTU-A nrPDB 28 0.71 5GP4-C nrPDB 27 2.3 2MAX-A nrPDB 27 2.6 4V0R-A nrPDB 26 3.8 5EHG-A nrPDB 25 7.0 5XGE-A nrPDB 25 7.6 2P3O-A nrPDB 25 9.6 >1WCM-J nrPDB Length = 70 Score = 145 bits (365), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 70/70 (100%), Positives = 70/70 (100%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF Sbjct: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 Query: 61 LRYNPLEKRD 70 LRYNPLEKRD Sbjct: 61 LRYNPLEKRD 70 >6A5U-J nrPDB Length = 72 Score = 115 bits (287), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 55/70 (78%), Positives = 62/70 (88%), Gaps = 1/70 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MI+PVRCFSCGKVVGDKW++YL LL+E + EG AL L LKRYCCRRM+LTHVDLIEKF Sbjct: 1 MIIPVRCFSCGKVVGDKWDAYLRLLEEGK-QEGDALDELKLKRYCCRRMVLTHVDLIEKF 59 Query: 61 LRYNPLEKRD 70 LRYNPLEK+D Sbjct: 60 LRYNPLEKKD 69 >3H0G-J nrPDB Length = 71 Score = 112 bits (280), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 52/69 (75%), Positives = 60/69 (86%), Gaps = 1/69 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MI+P+RCFSCGKV+GDKW++YL LLQED EG AL +LGL+RYCCRRMILTHVDLIEK Sbjct: 1 MIIPIRCFSCGKVIGDKWDTYLTLLQEDN-TEGEALDKLGLQRYCCRRMILTHVDLIEKL 59 Query: 61 LRYNPLEKR 69 L YNPL K+ Sbjct: 60 LCYNPLSKQ 68 >5IYC-J nrPDB Length = 67 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 50/68 (73%), Positives = 56/68 (82%), Gaps = 1/68 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MI+PVRCF+CGK+VG+KWE+YL LLQ E EG AL LGLKRYCCRRM+L HVDLIEK Sbjct: 1 MIIPVRCFTCGKIVGNKWEAYLGLLQA-EYTEGDALDALGLKRYCCRRMLLAHVDLIEKL 59 Query: 61 LRYNPLEK 68 L Y PLEK Sbjct: 60 LNYAPLEK 67 >2PMZ-N nrPDB Length = 66 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 30/64 (46%), Positives = 47/64 (73%), Gaps = 1/64 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 M++P+RCF+CG ++ DKW+S++ + E + G L LG+KRYCCRRM+L+HVD+I + Sbjct: 1 MLIPIRCFTCGSLIADKWQSFITRVNAGE-NPGKVLDDLGVKRYCCRRMLLSHVDIINEV 59 Query: 61 LRYN 64 + Y Sbjct: 60 IHYT 63 >2WAQ-N nrPDB Length = 66 Score = 75.5 bits (184), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 28/64 (43%), Positives = 46/64 (71%), Gaps = 1/64 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 M++P+RCF+CG ++ DKW+ ++ + E + G L LG+KRYCCRRM+L+H+D+I + Sbjct: 1 MMIPIRCFTCGSLIADKWQPFITRVNAGE-NPGKVLDDLGVKRYCCRRMLLSHIDIISEV 59 Query: 61 LRYN 64 + Y Sbjct: 60 IHYT 63 >4QIW-N nrPDB Length = 65 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 32/64 (50%), Positives = 46/64 (71%), Gaps = 1/64 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MIVPVRCF+CGKV+ DK+ + ++ E D G L LG++RYCCRR +L+HV+LI++ Sbjct: 1 MIVPVRCFTCGKVLADKYYEFKKRVEAGE-DPGKVLDDLGVERYCCRRTLLSHVELIDQV 59 Query: 61 LRYN 64 + Y Sbjct: 60 MVYK 63 >4LTU-A nrPDB Length = 107 Score = 28.5 bits (62), Expect = 0.71, Method: Compositional matrix adjust. Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Query: 7 CFSCGKVVGDKWESYLNLL--QEDELDEGTALSRLGLKRYCCRRMILTHVD 55 C +C V D W + L + ED+L +GTA RL R C+ I +D Sbjct: 46 CATCHVYVDDLWLAKLPPVDANEDDLLDGTASDRLPNSRLSCQIKIAPELD 96 >5GP4-C nrPDB Length = 468 Score = 26.9 bits (58), Expect = 2.3, Method: Composition-based stats. Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 2/47 (4%) Query: 24 LLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKFLRYNPLEKRD 70 L+++ LDEG SRL L +C M V+L++ L N ++K + Sbjct: 47 LVRDQLLDEGN--SRLNLATFCQTYMEPEAVELMKDTLEKNAIDKSE 91 >2MAX-A nrPDB Length = 126 Score = 26.6 bits (57), Expect = 2.6, Method: Compositional matrix adjust. Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 3/44 (6%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRY 44 M + RCF+C +G K+ L L+ E+EL + +G K Y Sbjct: 46 MNLSARCFNCLDKIGIKYVGELVLMSEEEL---KGVKNMGKKSY 86 >4V0R-A nrPDB Length = 892 Score = 26.2 bits (56), Expect = 3.8, Method: Composition-based stats. Identities = 10/23 (43%), Positives = 14/23 (60%) Query: 9 SCGKVVGDKWESYLNLLQEDELD 31 S G+ +G+KW+ LN L E D Sbjct: 1 SMGETLGEKWKKKLNQLSRKEFD 23 >5EHG-A nrPDB Length = 276 Score = 25.0 bits (53), Expect = 7.0, Method: Compositional matrix adjust. Identities = 10/23 (43%), Positives = 14/23 (60%) Query: 9 SCGKVVGDKWESYLNLLQEDELD 31 S G+ +G+KW+ LN L E D Sbjct: 4 SQGETLGEKWKKKLNQLSRKEFD 26 >5XGE-A nrPDB Length = 568 Score = 25.0 bits (53), Expect = 7.6, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 17/32 (53%) Query: 9 SCGKVVGDKWESYLNLLQEDELDEGTALSRLG 40 +CG GD+ + L + L EG L+RLG Sbjct: 190 TCGHAAGDELLRQVCTLLQQGLREGDTLARLG 221 >2P3O-A nrPDB Length = 305 Score = 24.6 bits (52), Expect = 9.6, Method: Composition-based stats. Identities = 9/20 (45%), Positives = 14/20 (70%) Query: 11 GKVVGDKWESYLNLLQEDEL 30 G+ +G+KW+S LN L + E Sbjct: 15 GETLGEKWKSRLNALGKSEF 34 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1WCM-J nrPDB 117 1e-27 6A5U-J nrPDB 111 8e-26 5IYC-J nrPDB 110 2e-25 3H0G-J nrPDB 109 2e-25 2PMZ-N nrPDB 107 1e-24 2WAQ-N nrPDB 106 2e-24 4QIW-N nrPDB 95 6e-21 Sequences not found previously or not previously below threshold: 3B6C-A nrPDB 26 3.4 4LTU-A nrPDB 26 3.5 CONVERGED! >1WCM-J nrPDB Length = 70 Score = 117 bits (293), Expect = 1e-27, Method: Composition-based stats. Identities = 70/70 (100%), Positives = 70/70 (100%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF Sbjct: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 Query: 61 LRYNPLEKRD 70 LRYNPLEKRD Sbjct: 61 LRYNPLEKRD 70 >6A5U-J nrPDB Length = 72 Score = 111 bits (277), Expect = 8e-26, Method: Composition-based stats. Identities = 55/70 (78%), Positives = 62/70 (88%), Gaps = 1/70 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MI+PVRCFSCGKVVGDKW++YL LL+E + EG AL L LKRYCCRRM+LTHVDLIEKF Sbjct: 1 MIIPVRCFSCGKVVGDKWDAYLRLLEEGK-QEGDALDELKLKRYCCRRMVLTHVDLIEKF 59 Query: 61 LRYNPLEKRD 70 LRYNPLEK+D Sbjct: 60 LRYNPLEKKD 69 >5IYC-J nrPDB Length = 67 Score = 110 bits (274), Expect = 2e-25, Method: Composition-based stats. Identities = 50/68 (73%), Positives = 56/68 (82%), Gaps = 1/68 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MI+PVRCF+CGK+VG+KWE+YL LLQ E EG AL LGLKRYCCRRM+L HVDLIEK Sbjct: 1 MIIPVRCFTCGKIVGNKWEAYLGLLQA-EYTEGDALDALGLKRYCCRRMLLAHVDLIEKL 59 Query: 61 LRYNPLEK 68 L Y PLEK Sbjct: 60 LNYAPLEK 67 >3H0G-J nrPDB Length = 71 Score = 109 bits (273), Expect = 2e-25, Method: Composition-based stats. Identities = 52/69 (75%), Positives = 60/69 (86%), Gaps = 1/69 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MI+P+RCFSCGKV+GDKW++YL LLQED EG AL +LGL+RYCCRRMILTHVDLIEK Sbjct: 1 MIIPIRCFSCGKVIGDKWDTYLTLLQEDN-TEGEALDKLGLQRYCCRRMILTHVDLIEKL 59 Query: 61 LRYNPLEKR 69 L YNPL K+ Sbjct: 60 LCYNPLSKQ 68 >2PMZ-N nrPDB Length = 66 Score = 107 bits (267), Expect = 1e-24, Method: Composition-based stats. Identities = 30/64 (46%), Positives = 47/64 (73%), Gaps = 1/64 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 M++P+RCF+CG ++ DKW+S++ + E + G L LG+KRYCCRRM+L+HVD+I + Sbjct: 1 MLIPIRCFTCGSLIADKWQSFITRVNAGE-NPGKVLDDLGVKRYCCRRMLLSHVDIINEV 59 Query: 61 LRYN 64 + Y Sbjct: 60 IHYT 63 >2WAQ-N nrPDB Length = 66 Score = 106 bits (265), Expect = 2e-24, Method: Composition-based stats. Identities = 28/64 (43%), Positives = 46/64 (71%), Gaps = 1/64 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 M++P+RCF+CG ++ DKW+ ++ + E + G L LG+KRYCCRRM+L+H+D+I + Sbjct: 1 MMIPIRCFTCGSLIADKWQPFITRVNAGE-NPGKVLDDLGVKRYCCRRMLLSHIDIISEV 59 Query: 61 LRYN 64 + Y Sbjct: 60 IHYT 63 >4QIW-N nrPDB Length = 65 Score = 95.0 bits (235), Expect = 6e-21, Method: Composition-based stats. Identities = 32/64 (50%), Positives = 46/64 (71%), Gaps = 1/64 (1%) Query: 1 MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKF 60 MIVPVRCF+CGKV+ DK+ + ++ E D G L LG++RYCCRR +L+HV+LI++ Sbjct: 1 MIVPVRCFTCGKVLADKYYEFKKRVEAGE-DPGKVLDDLGVERYCCRRTLLSHVELIDQV 59 Query: 61 LRYN 64 + Y Sbjct: 60 MVYK 63 >3B6C-A nrPDB Length = 234 Score = 26.1 bits (56), Expect = 3.4, Method: Composition-based stats. Identities = 12/49 (24%), Positives = 20/49 (40%) Query: 18 WESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKFLRYNPL 66 ++ L ++ E + G ++ RRM L H DL + PL Sbjct: 58 FDIVLTEVEVPEPEPGRWAEQVKEMCRSLRRMFLAHRDLARIAIDRVPL 106 >4LTU-A nrPDB Length = 107 Score = 26.1 bits (56), Expect = 3.5, Method: Composition-based stats. Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 2/51 (3%) Query: 7 CFSCGKVVGDKWESYLNLLQEDELD--EGTALSRLGLKRYCCRRMILTHVD 55 C +C V D W + L + +E D +GTA RL R C+ I +D Sbjct: 46 CATCHVYVDDLWLAKLPPVDANEDDLLDGTASDRLPNSRLSCQIKIAPELD 96 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.145 0.468 Lambda K H 0.267 0.0461 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,145,766 Number of Sequences: 36641 Number of extensions: 103029 Number of successful extensions: 324 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 22 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 290 Number of HSP's gapped (non-prelim): 28 length of query: 70 length of database: 10,336,785 effective HSP length: 41 effective length of query: 29 effective length of database: 8,834,504 effective search space: 256200616 effective search space used: 256200616 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.1 bits) S2: 53 (24.9 bits)