BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P21816 (200 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5EZW-A nrPDB 420 e-118 2IC1-A nrPDB 400 e-112 4QM9-A nrPDB 44 3e-05 4QMA-A nrPDB 40 9e-04 4TLF-A nrPDB 37 0.006 1Y3T-A nrPDB 30 0.70 5DBO-A nrPDB 26 9.1 2Y4T-A nrPDB 26 9.4 >5EZW-A nrPDB Length = 210 Score = 420 bits (1079), Expect = e-118, Method: Compositional matrix adjust. Identities = 199/200 (99%), Positives = 200/200 (100%) Query: 1 MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTR 60 MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTR Sbjct: 1 MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTR 60 Query: 61 NLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKK 120 NLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKK Sbjct: 61 NLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKK 120 Query: 121 SERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMT 180 SERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHL+SPPFDTCHAFDQRTGHKNKVTMT Sbjct: 121 SERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLHSPPFDTCHAFDQRTGHKNKVTMT 180 Query: 181 FHSKFGIRTPFTTSGSLENN 200 FHSKFGIRTPFTTSGSLENN Sbjct: 181 FHSKFGIRTPFTTSGSLENN 200 >2IC1-A nrPDB Length = 205 Score = 400 bits (1029), Expect = e-112, Method: Compositional matrix adjust. Identities = 184/200 (92%), Positives = 195/200 (97%) Query: 1 MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTR 60 ME+TE+LKPRTLADLIRILH+LFAGDEVNVEEVQA++EAYES+P EWA+YAKFDQYRYTR Sbjct: 6 MEQTEVLKPRTLADLIRILHQLFAGDEVNVEEVQAIMEAYESDPTEWAMYAKFDQYRYTR 65 Query: 61 NLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKK 120 NLVDQGNGKFNLMILCWGEGHGSSIHDHT+SHCFLK+LQGNLKETLF WPDKKSNEM+KK Sbjct: 66 NLVDQGNGKFNLMILCWGEGHGSSIHDHTNSHCFLKMLQGNLKETLFAWPDKKSNEMVKK 125 Query: 121 SERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMT 180 SER LRENQCAYINDSIGLHRVEN+SHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMT Sbjct: 126 SERVLRENQCAYINDSIGLHRVENISHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMT 185 Query: 181 FHSKFGIRTPFTTSGSLENN 200 FHSKFGIRTP TSGSLENN Sbjct: 186 FHSKFGIRTPNATSGSLENN 205 >4QM9-A nrPDB Length = 173 Score = 44.3 bits (103), Expect = 3e-05, Method: Compositional matrix adjust. Identities = 30/118 (25%), Positives = 61/118 (51%), Gaps = 8/118 (6%) Query: 54 DQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKK 113 DQY Y RN + + N + ++++ +++HDH S +L+G L +++ + Sbjct: 56 DQYAYGRNAIYRNN-ELEIIVINIPPNKETTVHDHGQSIGCAMVLEGKLLNSIY----RS 110 Query: 114 SNEMIKKSERT-LRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQR 170 + E + S + E +C + +H++ N + +E VSLH+YSPP + F+++ Sbjct: 111 TGEHAELSNSYFVHEGECLISTKGL-IHKMSNPT-SERMVSLHVYSPPLEDMTVFEEQ 166 >4QMA-A nrPDB Length = 208 Score = 39.7 bits (91), Expect = 9e-04, Method: Compositional matrix adjust. Identities = 28/130 (21%), Positives = 53/130 (40%), Gaps = 22/130 (16%) Query: 69 KFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWP---DKKSNEMIKKSERTL 125 +F+++ WG G + IHDHT + L G L+ + P D ++ L Sbjct: 78 RFSIVSFVWGPGQRTPIHDHT-----VWGLIGMLRGAEYSQPFVLDGSGRPVLHGEPTRL 132 Query: 126 RENQCAYINDSIG-LHRVENVSHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMTFHSK 184 ++ ++G +HRV N ++S+H+Y + V + +++ Sbjct: 133 EPGHVEAVSPTVGDIHRVHNAYDDRVSISIHVYG-------------ANIGGVRRSVYTE 179 Query: 185 FGIRTPFTTS 194 G R PF + Sbjct: 180 AGERKPFISG 189 >4TLF-A nrPDB Length = 211 Score = 37.0 bits (84), Expect = 0.006, Method: Compositional matrix adjust. Identities = 27/112 (24%), Positives = 49/112 (43%), Gaps = 10/112 (8%) Query: 51 AKFDQYRYTRNLVD-QGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDW 109 A+ D RY + L+ +F+++ WG G + +HDH + L G L+ + Sbjct: 53 ARPDPQRYQQYLLHVDSRQRFSVVSFVWGPGQITPVHDHR-----VWGLIGMLRGAEYSQ 107 Query: 110 P---DKKSNEMIKKSERTLRENQCAYINDSIG-LHRVENVSHTEPAVSLHLY 157 P D + R L + ++ IG +H+V N ++S+H+Y Sbjct: 108 PYAFDAGGRPHPSGARRRLEPGEVEALSPRIGDVHQVSNAFSDRTSISIHVY 159 >1Y3T-A nrPDB Length = 337 Score = 30.0 bits (66), Expect = 0.70, Method: Compositional matrix adjust. Identities = 30/112 (26%), Positives = 49/112 (43%), Gaps = 14/112 (12%) Query: 54 DQYRYTRNLVD-QGNGK-----FNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLF 107 ++Y + R + NG+ F +++L G+G +H H D+H + +L G L+ TL Sbjct: 24 ERYLFGRQVATVMANGRSTGDLFEIVLLSGGKGDAFPLHVHKDTHEGILVLDGKLELTL- 82 Query: 108 DWPDKKSNEMIKKSERTLRENQC-AYINDSIGLHRVENVSHTEPAVSLHLYS 158 D + +I + +Y S HR VS+T HLYS Sbjct: 83 ---DGERYLLISGDYANIPAGTPHSYRMQS---HRTRLVSYTMKGNVAHLYS 128 >5DBO-A nrPDB Length = 466 Score = 26.2 bits (56), Expect = 9.1, Method: Compositional matrix adjust. Identities = 16/45 (35%), Positives = 21/45 (46%), Gaps = 5/45 (11%) Query: 103 KETLFDWPDKKSNEMIKKSERTLRENQCAYIND-----SIGLHRV 142 KE L +W D NE I +E + N IND + G HR+ Sbjct: 250 KEGLLEWIDFLINERITLAEYVIARNAAQSINDGDTIVTYGRHRL 294 >2Y4T-A nrPDB Length = 450 Score = 26.2 bits (56), Expect = 9.4, Method: Compositional matrix adjust. Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 5/56 (8%) Query: 112 KKSNEMIKKSERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAF 167 KK N++I+ +E +R+ + Y + + E+V TEP+++ + CH F Sbjct: 255 KKLNKLIESAEELIRDGR--YTD---ATSKYESVMKTEPSIAEYTVRSKERICHCF 305 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5EZW-A nrPDB 365 e-102 2IC1-A nrPDB 363 e-101 4QMA-A nrPDB 148 1e-36 4QM9-A nrPDB 142 6e-35 Sequences not found previously or not previously below threshold: 4TLF-A nrPDB 116 8e-27 1Y3T-A nrPDB 35 0.027 3NT2-A nrPDB 31 0.40 6B4J-B nrPDB 30 0.67 1H78-A nrPDB 29 2.0 >5EZW-A nrPDB Length = 210 Score = 365 bits (936), Expect = e-102, Method: Composition-based stats. Identities = 199/200 (99%), Positives = 200/200 (100%) Query: 1 MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTR 60 MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTR Sbjct: 1 MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTR 60 Query: 61 NLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKK 120 NLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKK Sbjct: 61 NLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKK 120 Query: 121 SERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMT 180 SERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHL+SPPFDTCHAFDQRTGHKNKVTMT Sbjct: 121 SERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLHSPPFDTCHAFDQRTGHKNKVTMT 180 Query: 181 FHSKFGIRTPFTTSGSLENN 200 FHSKFGIRTPFTTSGSLENN Sbjct: 181 FHSKFGIRTPFTTSGSLENN 200 >2IC1-A nrPDB Length = 205 Score = 363 bits (931), Expect = e-101, Method: Composition-based stats. Identities = 184/200 (92%), Positives = 195/200 (97%) Query: 1 MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTR 60 ME+TE+LKPRTLADLIRILH+LFAGDEVNVEEVQA++EAYES+P EWA+YAKFDQYRYTR Sbjct: 6 MEQTEVLKPRTLADLIRILHQLFAGDEVNVEEVQAIMEAYESDPTEWAMYAKFDQYRYTR 65 Query: 61 NLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKK 120 NLVDQGNGKFNLMILCWGEGHGSSIHDHT+SHCFLK+LQGNLKETLF WPDKKSNEM+KK Sbjct: 66 NLVDQGNGKFNLMILCWGEGHGSSIHDHTNSHCFLKMLQGNLKETLFAWPDKKSNEMVKK 125 Query: 121 SERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMT 180 SER LRENQCAYINDSIGLHRVEN+SHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMT Sbjct: 126 SERVLRENQCAYINDSIGLHRVENISHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMT 185 Query: 181 FHSKFGIRTPFTTSGSLENN 200 FHSKFGIRTP TSGSLENN Sbjct: 186 FHSKFGIRTPNATSGSLENN 205 >4QMA-A nrPDB Length = 208 Score = 148 bits (374), Expect = 1e-36, Method: Composition-based stats. Identities = 29/132 (21%), Positives = 54/132 (40%), Gaps = 22/132 (16%) Query: 69 KFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWP---DKKSNEMIKKSERTL 125 +F+++ WG G + IHDHT + L G L+ + P D ++ L Sbjct: 78 RFSIVSFVWGPGQRTPIHDHT-----VWGLIGMLRGAEYSQPFVLDGSGRPVLHGEPTRL 132 Query: 126 RENQCAYINDSIG-LHRVENVSHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMTFHSK 184 ++ ++G +HRV N ++S+H+Y + V + +++ Sbjct: 133 EPGHVEAVSPTVGDIHRVHNAYDDRVSISIHVYG-------------ANIGGVRRSVYTE 179 Query: 185 FGIRTPFTTSGS 196 G R PF + S Sbjct: 180 AGERKPFISGYS 191 >4QM9-A nrPDB Length = 173 Score = 142 bits (359), Expect = 6e-35, Method: Composition-based stats. Identities = 30/118 (25%), Positives = 61/118 (51%), Gaps = 8/118 (6%) Query: 54 DQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKK 113 DQY Y RN + + N + ++++ +++HDH S +L+G L +++ + Sbjct: 56 DQYAYGRNAIYRNN-ELEIIVINIPPNKETTVHDHGQSIGCAMVLEGKLLNSIY----RS 110 Query: 114 SNEMIKKSERT-LRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQR 170 + E + S + E +C + +H++ N + +E VSLH+YSPP + F+++ Sbjct: 111 TGEHAELSNSYFVHEGECLISTKGL-IHKMSNPT-SERMVSLHVYSPPLEDMTVFEEQ 166 >4TLF-A nrPDB Length = 211 Score = 116 bits (290), Expect = 8e-27, Method: Composition-based stats. Identities = 33/151 (21%), Positives = 57/151 (37%), Gaps = 23/151 (15%) Query: 51 AKFDQYRYTRNLVD-QGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDW 109 A+ D RY + L+ +F+++ WG G + +HDH + L G L+ + Sbjct: 53 ARPDPQRYQQYLLHVDSRQRFSVVSFVWGPGQITPVHDHR-----VWGLIGMLRGAEYSQ 107 Query: 110 P---DKKSNEMIKKSERTLRENQCAYINDSIG-LHRVENVSHTEPAVSLHLYSPPFDTCH 165 P D + R L + ++ IG +H+V N ++S+H+Y Sbjct: 108 PYAFDAGGRPHPSGARRRLEPGEVEALSPRIGDVHQVSNAFSDRTSISIHVYG------- 160 Query: 166 AFDQRTGHKNKVTMTFHSKFGIRTPFTTSGS 196 + V S G PF + S Sbjct: 161 ------ANIGAVRRAVFSAEGEEKPFISGYS 185 >1Y3T-A nrPDB Length = 337 Score = 34.7 bits (78), Expect = 0.027, Method: Composition-based stats. Identities = 32/146 (21%), Positives = 55/146 (37%), Gaps = 23/146 (15%) Query: 54 DQYRYTRN-LVDQGNGK-----FNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLF 107 ++Y + R NG+ F +++L G+G +H H D+H + +L G L+ TL Sbjct: 24 ERYLFGRQVATVMANGRSTGDLFEIVLLSGGKGDAFPLHVHKDTHEGILVLDGKLELTL- 82 Query: 108 DWPDKKSNEMIKKSERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLYS--------- 158 D + +I + + + HR VS+T HLYS Sbjct: 83 ---DGERYLLISGDYANIPAG--TPHSYRMQSHRTRLVSYTMKGNVAHLYSVIGNPYDHA 137 Query: 159 --PPFDTCHAFDQRTGHKNKVTMTFH 182 PP+ + ++R V Sbjct: 138 EHPPYASEEVSNERFAEAAAVATIVF 163 >3NT2-A nrPDB Length = 344 Score = 30.9 bits (68), Expect = 0.40, Method: Composition-based stats. Identities = 26/146 (17%), Positives = 52/146 (35%), Gaps = 41/146 (28%) Query: 21 ELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTRNLVDQGNGKFNLMILCWGEG 80 E+ A +VN E Q V+E Y+ N +Y D N VD +++ WG Sbjct: 29 EIVAVTDVNQEAAQKVVEQYQLNAT---VYPNDDSLLADEN-VD------AVLVTSWGPA 78 Query: 81 HGSSIHD--------------HTDSHCFLKLLQGNLK--------------ETLFDWPDK 112 H SS+ T + +++++ +K ++ + + Sbjct: 79 HESSVLKAIKAQKYVFCEKPLATTAEGCMRIVEEEIKVGKRLVQVGFMRRYDSGYVQLKE 138 Query: 113 KSNEMIKKSERTLRENQCAYINDSIG 138 + + + CA+ N ++G Sbjct: 139 ALDNHVIGEPLMI---HCAHRNPTVG 161 >6B4J-B nrPDB Length = 317 Score = 30.1 bits (66), Expect = 0.67, Method: Composition-based stats. Identities = 9/28 (32%), Positives = 14/28 (50%) Query: 72 LMILCWGEGHGSSIHDHTDSHCFLKLLQ 99 ++ L W G+ IH H +H + L Q Sbjct: 199 IIQLRWPYGNRQEIHPHGLNHGWRWLAQ 226 >1H78-A nrPDB Length = 605 Score = 28.6 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 11/41 (26%), Positives = 18/41 (43%) Query: 40 YESNPAEWALYAKFDQYRYTRNLVDQGNGKFNLMILCWGEG 80 Y + E +Y F Y Y N + NG+ + + +G G Sbjct: 162 YAQSKTEKDVYDAFQAYEYEVNTLFSSNGQTPFVTITFGTG 202 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.306 0.127 0.340 Lambda K H 0.267 0.0390 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,485,169 Number of Sequences: 36641 Number of extensions: 285411 Number of successful extensions: 766 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 736 Number of HSP's gapped (non-prelim): 19 length of query: 200 length of database: 10,336,785 effective HSP length: 92 effective length of query: 108 effective length of database: 6,965,813 effective search space: 752307804 effective search space used: 752307804 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 56 (26.3 bits)