BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P21374 (235 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6J6Q-H nrPDB 488 e-138 6FF7-w nrPDB 72 2e-13 5YZG-y nrPDB 72 2e-13 1X4T-A nrPDB 49 1e-06 3LHP-S nrPDB 32 0.30 6AF0-A nrPDB 31 0.37 6DJP-B nrPDB 28 2.9 2AYI-A nrPDB 27 7.3 >6J6Q-H nrPDB Length = 235 Score = 488 bits (1255), Expect = e-138, Method: Compositional matrix adjust. Identities = 235/235 (100%), Positives = 235/235 (100%) Query: 1 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ 60 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ Sbjct: 1 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ 60 Query: 61 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG 120 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG Sbjct: 61 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG 120 Query: 121 KLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINTKCIPKDRNDFYYHGKVTAALTEFE 180 KLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINTKCIPKDRNDFYYHGKVTAALTEFE Sbjct: 121 KLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINTKCIPKDRNDFYYHGKVTAALTEFE 180 Query: 181 ANWTSILKAHYNVPVNEDEEEMSRQTQEIHVPTLADMEHWLVQRRKKKLMDELNL 235 ANWTSILKAHYNVPVNEDEEEMSRQTQEIHVPTLADMEHWLVQRRKKKLMDELNL Sbjct: 181 ANWTSILKAHYNVPVNEDEEEMSRQTQEIHVPTLADMEHWLVQRRKKKLMDELNL 235 >6FF7-w nrPDB Length = 285 Score = 72.0 bits (175), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 49/147 (33%), Positives = 77/147 (52%), Gaps = 12/147 (8%) Query: 1 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ 60 M+RN +KA + L RF++ Q E G K+ +RP S+ + +A +W+RQ+ EI + Sbjct: 1 MARNAEKAMTALARFRQAQLEE-GKVKE----RRPFLASECTELPKAEKWRRQIIGEISK 55 Query: 61 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG 120 K +I + L E +I +LNDE+N L +E W+ I K +L + G Sbjct: 56 KVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKV----GPKMLDHEG 111 Query: 121 KLINGKR---YFGRALELPEVKEWLKQ 144 K + G R YFG A +LP V+E ++ Sbjct: 112 KEVPGNRGYKYFGAAKDLPGVRELFEK 138 >5YZG-y nrPDB Length = 307 Score = 72.0 bits (175), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 52/151 (34%), Positives = 80/151 (52%), Gaps = 13/151 (8%) Query: 1 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ 60 M+RN +KA + L RF++ Q E G K+ +RP S+ + +A +W+RQ+ EI + Sbjct: 1 MARNAEKAMTALARFRQAQLEE-GKVKE----RRPFLASECTELPKAEKWRRQIIGEISK 55 Query: 61 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG 120 K +I + L E +I +LNDE+N L +E W+ I K +L + G Sbjct: 56 KVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKV----GPKMLDHEG 111 Query: 121 KLINGKR---YFGRALELPEVKEWLKQSQRQ 148 K + G R YFG A +LP V+E L + +RQ Sbjct: 112 KEVPGNRGYKYFGAAKDLPGVRE-LFEKERQ 141 >1X4T-A nrPDB Length = 92 Score = 49.3 bits (116), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 23/64 (35%), Positives = 38/64 (59%) Query: 33 QRPRNVSKVKSIKEANEWKRQVSKEIKQKSTRIYDPSLNEMQIAELNDELNNLFKEWKRW 92 +RP S+ + +A +W+RQ+ EI +K +I + L E +I +LNDE+N L +E W Sbjct: 12 RRPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHW 71 Query: 93 QWHI 96 + I Sbjct: 72 EVRI 75 >3LHP-S nrPDB Length = 123 Score = 31.6 bits (70), Expect = 0.30, Method: Compositional matrix adjust. Identities = 22/86 (25%), Positives = 41/86 (47%), Gaps = 11/86 (12%) Query: 138 VKEWLKQSQRQNDGGSINTKCIPKDRNDFYYHGKVTAALTEFEANWTSILKAHYNVPVNE 197 +K+WLK + + + + + + +D ND K A + E NW I ++ ++V Sbjct: 48 IKDWLKIVRGEAEQARVAVRNVGRDAND-----KAAALGKDKEINWFDISQSLWDV---- 98 Query: 198 DEEEMSRQTQEIHVPTLADMEHWLVQ 223 ++++ + LADME WL Q Sbjct: 99 --QKLTDAAIKKIEAALADMEAWLTQ 122 >6AF0-A nrPDB Length = 939 Score = 31.2 bits (69), Expect = 0.37, Method: Compositional matrix adjust. Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 2/50 (4%) Query: 107 RKRLEDSHVLMNSGKLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINT 156 R+ ++D+HV +N G + R F +A+E E+ L + + ND G I+ Sbjct: 767 RETIQDAHVYVNMGHIYAELRQFSKAIESYEIA--LSKEGKANDAGIISC 814 >6DJP-B nrPDB Length = 639 Score = 28.5 bits (62), Expect = 2.9, Method: Compositional matrix adjust. Identities = 15/60 (25%), Positives = 31/60 (51%), Gaps = 1/60 (1%) Query: 39 SKVKSIKEANEWKRQVSKEIKQKSTRIYDPSLNEMQIAELNDELNNLFK-EWKRWQWHID 97 SK+ I N+ + + KST + PSL ++ +++ +N +F + K++ W+ D Sbjct: 252 SKLAGIVVPNDGNCHLKNNVYVKSTTMEHPSLGQLSEKLIDNNINVIFAVQGKQFHWYKD 311 >2AYI-A nrPDB Length = 408 Score = 26.9 bits (58), Expect = 7.3, Method: Compositional matrix adjust. Identities = 18/49 (36%), Positives = 23/49 (46%), Gaps = 5/49 (10%) Query: 175 ALTEFEANWTSILKAHYN-----VPVNEDEEEMSRQTQEIHVPTLADME 218 A+TEF NWT + AH P +EE + R + I T AD E Sbjct: 134 AITEFVTNWTIVPFAHPGWARAVFPGLPEEEAVRRLWEAIFQATRADQE 182 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6J6Q-H nrPDB 394 e-110 5YZG-y nrPDB 198 1e-51 6FF7-w nrPDB 196 6e-51 1X4T-A nrPDB 132 8e-32 Sequences not found previously or not previously below threshold: 3GBX-A nrPDB 35 0.029 3G8M-A nrPDB 34 0.045 4P3M-A nrPDB 31 0.34 4U8U-C nrPDB 29 2.1 5WDE-A nrPDB 28 2.9 4D18-G nrPDB 28 3.3 5Y86-A nrPDB 28 4.3 5LCL-B nrPDB 27 7.3 4CXU-A nrPDB 27 7.5 CONVERGED! >6J6Q-H nrPDB Length = 235 Score = 394 bits (1012), Expect = e-110, Method: Composition-based stats. Identities = 235/235 (100%), Positives = 235/235 (100%) Query: 1 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ 60 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ Sbjct: 1 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ 60 Query: 61 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG 120 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG Sbjct: 61 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG 120 Query: 121 KLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINTKCIPKDRNDFYYHGKVTAALTEFE 180 KLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINTKCIPKDRNDFYYHGKVTAALTEFE Sbjct: 121 KLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINTKCIPKDRNDFYYHGKVTAALTEFE 180 Query: 181 ANWTSILKAHYNVPVNEDEEEMSRQTQEIHVPTLADMEHWLVQRRKKKLMDELNL 235 ANWTSILKAHYNVPVNEDEEEMSRQTQEIHVPTLADMEHWLVQRRKKKLMDELNL Sbjct: 181 ANWTSILKAHYNVPVNEDEEEMSRQTQEIHVPTLADMEHWLVQRRKKKLMDELNL 235 >5YZG-y nrPDB Length = 307 Score = 198 bits (504), Expect = 1e-51, Method: Composition-based stats. Identities = 49/150 (32%), Positives = 79/150 (52%), Gaps = 12/150 (8%) Query: 1 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ 60 M+RN +KA + L RF++ Q E G K+ +RP S+ + +A +W+RQ+ EI + Sbjct: 1 MARNAEKAMTALARFRQAQLEE-GKVKE----RRPFLASECTELPKAEKWRRQIIGEISK 55 Query: 61 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG 120 K +I + L E +I +LNDE+N L +E W+ I K +L + G Sbjct: 56 KVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKV----GPKMLDHEG 111 Query: 121 KLINGKR---YFGRALELPEVKEWLKQSQR 147 K + G R YFG A +LP V+E ++ ++ Sbjct: 112 KEVPGNRGYKYFGAAKDLPGVRELFEKERQ 141 >6FF7-w nrPDB Length = 285 Score = 196 bits (498), Expect = 6e-51, Method: Composition-based stats. Identities = 49/147 (33%), Positives = 77/147 (52%), Gaps = 12/147 (8%) Query: 1 MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQ 60 M+RN +KA + L RF++ Q E G K+ +RP S+ + +A +W+RQ+ EI + Sbjct: 1 MARNAEKAMTALARFRQAQLEE-GKVKE----RRPFLASECTELPKAEKWRRQIIGEISK 55 Query: 61 KSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSG 120 K +I + L E +I +LNDE+N L +E W+ I K +L + G Sbjct: 56 KVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKV----GPKMLDHEG 111 Query: 121 KLINGKR---YFGRALELPEVKEWLKQ 144 K + G R YFG A +LP V+E ++ Sbjct: 112 KEVPGNRGYKYFGAAKDLPGVRELFEK 138 >1X4T-A nrPDB Length = 92 Score = 132 bits (333), Expect = 8e-32, Method: Composition-based stats. Identities = 27/86 (31%), Positives = 44/86 (51%), Gaps = 4/86 (4%) Query: 22 SAGGYKDYSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQKSTRIYDPSLNEMQIAELNDE 81 S+G K+ +RP S+ + +A +W+RQ+ EI +K +I + L E +I +LNDE Sbjct: 5 SSGKVKE----RRPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDE 60 Query: 82 LNNLFKEWKRWQWHIDHTLMEKKTKR 107 +N L +E W+ I K Sbjct: 61 INKLLREKGHWEVRIKELGGPDYGKV 86 >3GBX-A nrPDB Length = 420 Score = 35.1 bits (79), Expect = 0.029, Method: Composition-based stats. Identities = 19/73 (26%), Positives = 34/73 (46%), Gaps = 3/73 (4%) Query: 72 EMQIAELNDEL-NNLFKEWKRWQWHIDHTLMEKKT--KRKRLEDSHVLMNSGKLINGKRY 128 EM IA+ + EL + +E R + HI+ E T + + + S + + GKRY Sbjct: 8 EMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRY 67 Query: 129 FGRALELPEVKEW 141 +G + V++ Sbjct: 68 YGGCEYVDVVEQL 80 >3G8M-A nrPDB Length = 417 Score = 34.3 bits (77), Expect = 0.045, Method: Composition-based stats. Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 3/62 (4%) Query: 72 EMQIAELNDEL-NNLFKEWKRWQWHIDHTLMEKKT--KRKRLEDSHVLMNSGKLINGKRY 128 EM IA+ + EL + +E R + HI+ E T + + + S + + GKRY Sbjct: 5 EMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVMQAQGSQLTNKFAEGYPGKRY 64 Query: 129 FG 130 +G Sbjct: 65 YG 66 >4P3M-A nrPDB Length = 429 Score = 31.2 bits (69), Expect = 0.34, Method: Composition-based stats. Identities = 17/62 (27%), Positives = 30/62 (48%), Gaps = 3/62 (4%) Query: 72 EMQIAELNDEL-NNLFKEWKRWQWHIDHTLMEKKT--KRKRLEDSHVLMNSGKLINGKRY 128 +M IA+ + EL ++ E +R + HI+ E T + + S + + GKRY Sbjct: 5 DMNIADYDPELWQSITDEVQRQEDHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRY 64 Query: 129 FG 130 +G Sbjct: 65 YG 66 >4U8U-C nrPDB Length = 151 Score = 28.9 bits (63), Expect = 2.1, Method: Composition-based stats. Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 5/50 (10%) Query: 111 EDSHVLMNSGKLINGKRYFGRALELPEVKEWLKQSQRQN-DGGSINTKCI 159 E S V + G+LI K A E+PEVKE + N +GG + C+ Sbjct: 25 ESSKVKIAFGRLILTK----LAKEIPEVKELFNKVDIDNPEGGPFSAHCL 70 >5WDE-A nrPDB Length = 330 Score = 28.2 bits (61), Expect = 2.9, Method: Composition-based stats. Identities = 10/30 (33%), Positives = 18/30 (60%), Gaps = 2/30 (6%) Query: 62 STRIYDPSLNEMQIAELNDELNNLFKEWKR 91 S ++Y P L E Q+ + D++N +F E+ Sbjct: 163 SGQLYVPGLTEFQVQSV-DDINKVF-EFGH 190 >4D18-G nrPDB Length = 222 Score = 28.2 bits (61), Expect = 3.3, Method: Composition-based stats. Identities = 8/28 (28%), Positives = 14/28 (50%) Query: 116 LMNSGKLINGKRYFGRALELPEVKEWLK 143 L++ G FG L++P V+E + Sbjct: 34 LIHQVLEAPGVYVFGELLDMPNVRELAE 61 >5Y86-A nrPDB Length = 588 Score = 27.8 bits (60), Expect = 4.3, Method: Composition-based stats. Identities = 12/58 (20%), Positives = 24/58 (41%), Gaps = 5/58 (8%) Query: 84 NLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMNSGKLINGKRYFGRALELPEVKEW 141 L ++ KR ++ I+ + + D V+ L+ G+ G+ P K+W Sbjct: 431 KLLEQSKRAKYFINSKGIPRYCSVTTQADGRVV-----LVGGRSRRGKKRGPPGSKDW 483 >5LCL-B nrPDB Length = 371 Score = 27.0 bits (58), Expect = 7.3, Method: Composition-based stats. Identities = 13/55 (23%), Positives = 25/55 (45%), Gaps = 1/55 (1%) Query: 48 NEWKRQVSKEIKQKSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLME 102 EW+R+ + ++ + Y+ + EM++ E N +E K + HI H Sbjct: 289 EEWQRREEGKAHRREKK-YEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDP 342 >4CXU-A nrPDB Length = 536 Score = 27.0 bits (58), Expect = 7.5, Method: Composition-based stats. Identities = 10/30 (33%), Positives = 15/30 (50%) Query: 113 SHVLMNSGKLINGKRYFGRALELPEVKEWL 142 +L +G GKR+ GR + P + WL Sbjct: 412 PTLLDLAGVRHPGKRWRGREIAEPRGRSWL 441 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.129 0.362 Lambda K H 0.267 0.0400 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,376,942 Number of Sequences: 36641 Number of extensions: 366635 Number of successful extensions: 908 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 24 Number of HSP's that attempted gapping in prelim test: 875 Number of HSP's gapped (non-prelim): 45 length of query: 235 length of database: 10,336,785 effective HSP length: 94 effective length of query: 141 effective length of database: 6,892,531 effective search space: 971846871 effective search space used: 971846871 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 57 (26.6 bits)