BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P20809 (199 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4MHL-A nrPDB 331 1e-91 3VEM-A nrPDB 31 0.36 2D7R-A nrPDB 28 3.3 3CCV-V nrPDB 27 7.0 >4MHL-A nrPDB Length = 177 Score = 331 bits (848), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 177/177 (100%), Positives = 177/177 (100%) Query: 23 GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSA 82 GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSA Sbjct: 1 GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSA 60 Query: 83 GALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQL 142 GALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQL Sbjct: 61 GALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQL 120 Query: 143 LMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL 199 LMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL Sbjct: 121 LMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL 177 >3VEM-A nrPDB Length = 115 Score = 30.8 bits (68), Expect = 0.36, Method: Compositional matrix adjust. Identities = 14/43 (32%), Positives = 23/43 (53%) Query: 104 YLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSR 146 +L ++ LRR +S KT E + L+A L+R + +Q R Sbjct: 37 FLHELEKLRRESENSKKTFEEKKSILKAELERKMAEVQAEFRR 79 >2D7R-A nrPDB Length = 570 Score = 27.7 bits (60), Expect = 3.3, Method: Compositional matrix adjust. Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 3/36 (8%) Query: 137 LRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIR 172 + + M+++A P PP+ PP++AWG IR Sbjct: 399 CKSFKWFMTKIAWDLPKF---YPPVEPPAAAWGEIR 431 >3CCV-V nrPDB Length = 71 Score = 26.6 bits (57), Expect = 7.0, Method: Compositional matrix adjust. Identities = 22/76 (28%), Positives = 36/76 (47%), Gaps = 19/76 (25%) Query: 59 QLRDKFPADGDHNLDSLPTLAMSAGAL----GALQLPGVLTRLRADLLSYLRHVQWLRRA 114 ++RD PA+ + LD L T ++A A+ GA + PG + LR + Sbjct: 8 EIRDMTPAEREAELDDLKTELLNARAVQAAGGAPENPGRIKELRKAI------------- 54 Query: 115 GGSSLKTLEPELGTLQ 130 + +KT++ E G LQ Sbjct: 55 --ARIKTIQGEEGDLQ 68 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4MHL-A nrPDB 290 3e-79 Sequences not found previously or not previously below threshold: 2D7R-A nrPDB 30 0.60 3CCV-V nrPDB 28 2.3 3VEM-A nrPDB 27 4.8 6CNN-A nrPDB 27 6.6 CONVERGED! >4MHL-A nrPDB Length = 177 Score = 290 bits (743), Expect = 3e-79, Method: Composition-based stats. Identities = 177/177 (100%), Positives = 177/177 (100%) Query: 23 GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSA 82 GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSA Sbjct: 1 GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSA 60 Query: 83 GALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQL 142 GALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQL Sbjct: 61 GALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQL 120 Query: 143 LMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL 199 LMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL Sbjct: 121 LMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL 177 >2D7R-A nrPDB Length = 570 Score = 30.4 bits (67), Expect = 0.60, Method: Composition-based stats. Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 10/42 (23%) Query: 159 PPLAPPSSAWGGIR---------AAHAILGGLHLTLDWAVRG 191 PP+ PP++AWG IR H LG L L+ VRG Sbjct: 418 PPVEPPAAAWGEIRNVGTGLCADTKHGALGS-PLRLEGCVRG 458 >3CCV-V nrPDB Length = 71 Score = 28.4 bits (62), Expect = 2.3, Method: Composition-based stats. Identities = 22/76 (28%), Positives = 36/76 (47%), Gaps = 19/76 (25%) Query: 59 QLRDKFPADGDHNLDSLPTLAMSAGAL----GALQLPGVLTRLRADLLSYLRHVQWLRRA 114 ++RD PA+ + LD L T ++A A+ GA + PG + LR + Sbjct: 8 EIRDMTPAEREAELDDLKTELLNARAVQAAGGAPENPGRIKELRKAI------------- 54 Query: 115 GGSSLKTLEPELGTLQ 130 + +KT++ E G LQ Sbjct: 55 --ARIKTIQGEEGDLQ 68 >3VEM-A nrPDB Length = 115 Score = 27.3 bits (59), Expect = 4.8, Method: Composition-based stats. Identities = 14/43 (32%), Positives = 23/43 (53%) Query: 104 YLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSR 146 +L ++ LRR +S KT E + L+A L+R + +Q R Sbjct: 37 FLHELEKLRRESENSKKTFEEKKSILKAELERKMAEVQAEFRR 79 >6CNN-A nrPDB Length = 427 Score = 26.9 bits (58), Expect = 6.6, Method: Composition-based stats. Identities = 14/42 (33%), Positives = 22/42 (52%) Query: 111 LRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQP 152 L++ SS + LE ++ TL +LD L L + LP+P Sbjct: 381 LQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEP 422 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.139 0.431 Lambda K H 0.267 0.0427 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,622,924 Number of Sequences: 36641 Number of extensions: 334646 Number of successful extensions: 1625 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 33 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 1392 Number of HSP's gapped (non-prelim): 205 length of query: 199 length of database: 10,336,785 effective HSP length: 92 effective length of query: 107 effective length of database: 6,965,813 effective search space: 745341991 effective search space used: 745341991 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 57 (26.5 bits)