BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P20486 (150 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1QB3-A nrPDB 311 6e-86 3QY2-A nrPDB 249 3e-67 1PUC-A nrPDB 157 1e-39 4Y72-C nrPDB 90 4e-19 1BUH-B nrPDB 89 7e-19 2ASS-C nrPDB 89 9e-19 5M0X-A nrPDB 29 0.71 1YNB-A nrPDB 28 2.1 5C6Q-A nrPDB 27 2.6 3ODT-A nrPDB 26 7.9 >1QB3-A nrPDB Length = 150 Score = 311 bits (798), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 1 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN 60 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN Sbjct: 1 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN 60 Query: 61 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAAAQQQ 120 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAAAQQQ Sbjct: 61 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAAAQQQ 120 Query: 121 QQQQQQQQQQQQQHQTQSISNDMQVPPQIS 150 QQQQQQQQQQQQQHQTQSISNDMQVPPQIS Sbjct: 121 QQQQQQQQQQQQQHQTQSISNDMQVPPQIS 150 >3QY2-A nrPDB Length = 117 Score = 249 bits (636), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 116/117 (99%), Positives = 116/117 (99%) Query: 1 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN 60 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN Sbjct: 1 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN 60 Query: 61 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAAA 117 SEVGTLRILTEDEWRGLGITQSLGWEHYECHA EPHILLFKRPLNYEAELRAATAAA Sbjct: 61 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAAEPHILLFKRPLNYEAELRAATAAA 117 >1PUC-A nrPDB Length = 105 Score = 157 bits (397), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 73/96 (76%), Positives = 78/96 (81%) Query: 11 RKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILT 70 R LT ER R+ F D IHYSPRY+DD YEYRHVMLPKAMLK IP+DYFN E GTLRIL Sbjct: 7 RLLTASERERLEPFIDQIHYSPRYADDEYEYRHVMLPKAMLKAIPTDYFNPETGTLRILQ 66 Query: 71 EDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNY 106 E+EWRGLGITQSLGWE YE H PEPHILLFKR +Y Sbjct: 67 EEEWRGLGITQSLGWEMYEVHVPEPHILLFKREKDY 102 >4Y72-C nrPDB Length = 84 Score = 89.7 bits (221), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 41/77 (53%), Positives = 55/77 (71%), Gaps = 9/77 (11%) Query: 28 IHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGITQSLGWEH 87 I+YS +Y D++YEYRHVMLP+ + K +P + +++E+EWR LG+ QSLGW H Sbjct: 11 IYYSDKYFDEHYEYRHVMLPRELSKQVPKTH---------LMSEEEWRRLGVQQSLGWVH 61 Query: 88 YECHAPEPHILLFKRPL 104 Y H PEPHILLF+RPL Sbjct: 62 YMIHEPEPHILLFRRPL 78 >1BUH-B nrPDB Length = 79 Score = 89.0 bits (219), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 40/77 (51%), Positives = 53/77 (68%), Gaps = 9/77 (11%) Query: 28 IHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGITQSLGWEH 87 I+YS +Y D+ +EYRHVMLPK + K++P + +++E EWR LG+ QS GW H Sbjct: 6 IYYSDKYDDEEFEYRHVMLPKDIAKLVPKTH---------LMSESEWRNLGVQQSQGWVH 56 Query: 88 YECHAPEPHILLFKRPL 104 Y H PEPHILLF+RPL Sbjct: 57 YMIHEPEPHILLFRRPL 73 >2ASS-C nrPDB Length = 69 Score = 88.6 bits (218), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 40/77 (51%), Positives = 53/77 (68%), Gaps = 9/77 (11%) Query: 28 IHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGITQSLGWEH 87 I+YS +Y D+ +EYRHVMLPK + K++P + +++E EWR LG+ QS GW H Sbjct: 2 IYYSDKYDDEEFEYRHVMLPKDIAKLVPKTH---------LMSESEWRNLGVQQSQGWVH 52 Query: 88 YECHAPEPHILLFKRPL 104 Y H PEPHILLF+RPL Sbjct: 53 YMIHEPEPHILLFRRPL 69 >5M0X-A nrPDB Length = 575 Score = 29.3 bits (64), Expect = 0.71, Method: Composition-based stats. Identities = 12/41 (29%), Positives = 22/41 (53%) Query: 26 DSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTL 66 D+ + P D N+ Y ++P + + + SDYF +E G + Sbjct: 95 DAFSFKPPEIDPNWRYTASVVPDVLERNLQSDYFVAEEGKV 135 >1YNB-A nrPDB Length = 173 Score = 27.7 bits (60), Expect = 2.1, Method: Compositional matrix adjust. Identities = 22/60 (36%), Positives = 29/60 (48%), Gaps = 3/60 (5%) Query: 59 FNSEVGTLRILTEDEWRGLGIT--QSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAA 116 F EVG+L++ W LGI +S+ EH A IL K + E +AATAA Sbjct: 13 FIHEVGSLKLTPRSGWLKLGIRLPESVA-EHNFRAAIIAFILALKSGESVEKACKAATAA 71 >5C6Q-A nrPDB Length = 209 Score = 27.3 bits (59), Expect = 2.6, Method: Compositional matrix adjust. Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 2/60 (3%) Query: 37 DNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPH 96 D R +ML + + K+I ++ E L R L I QSL W+H C P P+ Sbjct: 135 DTKSARSIMLIE-LKKLIEANPLFREKLVFPTLKASRLRTL-INQSLNWQHQLCKNPRPN 192 >3ODT-A nrPDB Length = 313 Score = 25.8 bits (55), Expect = 7.9, Method: Compositional matrix adjust. Identities = 12/38 (31%), Positives = 22/38 (57%), Gaps = 1/38 (2%) Query: 64 GTLRILTEDE-WRGLGITQSLGWEHYECHAPEPHILLF 100 GT+R+ ++D+ W G + G+ + C+ E +LLF Sbjct: 39 GTVRLWSKDDQWLGTVVYTGQGFLNSVCYDSEKELLLF 76 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1QB3-A nrPDB 218 7e-58 3QY2-A nrPDB 205 5e-54 1PUC-A nrPDB 157 2e-39 2ASS-C nrPDB 105 1e-23 1BUH-B nrPDB 104 2e-23 4Y72-C nrPDB 101 1e-22 Sequences not found previously or not previously below threshold: 4QIW-A nrPDB 29 0.92 5C6Q-A nrPDB 29 1.2 3QQC-A nrPDB 28 1.3 6APH-A nrPDB 28 1.4 2M1M-A nrPDB 28 1.8 3ODT-A nrPDB 27 2.4 3RQ4-A nrPDB 26 5.4 3N58-A nrPDB 26 5.6 5LUG-A nrPDB 26 6.0 5HUQ-A nrPDB 26 9.1 5HW4-A nrPDB 26 9.5 CONVERGED! >1QB3-A nrPDB Length = 150 Score = 218 bits (555), Expect = 7e-58, Method: Composition-based stats. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 1 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN 60 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN Sbjct: 1 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN 60 Query: 61 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAAAQQQ 120 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAAAQQQ Sbjct: 61 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAAAQQQ 120 Query: 121 QQQQQQQQQQQQQHQTQSISNDMQVPPQIS 150 QQQQQQQQQQQQQHQTQSISNDMQVPPQIS Sbjct: 121 QQQQQQQQQQQQQHQTQSISNDMQVPPQIS 150 >3QY2-A nrPDB Length = 117 Score = 205 bits (522), Expect = 5e-54, Method: Composition-based stats. Identities = 116/117 (99%), Positives = 116/117 (99%) Query: 1 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN 60 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN Sbjct: 1 MYHHYHAFQGRKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFN 60 Query: 61 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYEAELRAATAAA 117 SEVGTLRILTEDEWRGLGITQSLGWEHYECHA EPHILLFKRPLNYEAELRAATAAA Sbjct: 61 SEVGTLRILTEDEWRGLGITQSLGWEHYECHAAEPHILLFKRPLNYEAELRAATAAA 117 >1PUC-A nrPDB Length = 105 Score = 157 bits (397), Expect = 2e-39, Method: Composition-based stats. Identities = 73/97 (75%), Positives = 79/97 (81%) Query: 11 RKLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILT 70 R LT ER R+ F D IHYSPRY+DD YEYRHVMLPKAMLK IP+DYFN E GTLRIL Sbjct: 7 RLLTASERERLEPFIDQIHYSPRYADDEYEYRHVMLPKAMLKAIPTDYFNPETGTLRILQ 66 Query: 71 EDEWRGLGITQSLGWEHYECHAPEPHILLFKRPLNYE 107 E+EWRGLGITQSLGWE YE H PEPHILLFKR +Y+ Sbjct: 67 EEEWRGLGITQSLGWEMYEVHVPEPHILLFKREKDYQ 103 >2ASS-C nrPDB Length = 69 Score = 105 bits (261), Expect = 1e-23, Method: Composition-based stats. Identities = 40/78 (51%), Positives = 53/78 (67%), Gaps = 9/78 (11%) Query: 27 SIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGITQSLGWE 86 I+YS +Y D+ +EYRHVMLPK + K++P + +++E EWR LG+ QS GW Sbjct: 1 QIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTH---------LMSESEWRNLGVQQSQGWV 51 Query: 87 HYECHAPEPHILLFKRPL 104 HY H PEPHILLF+RPL Sbjct: 52 HYMIHEPEPHILLFRRPL 69 >1BUH-B nrPDB Length = 79 Score = 104 bits (258), Expect = 2e-23, Method: Composition-based stats. Identities = 40/78 (51%), Positives = 53/78 (67%), Gaps = 9/78 (11%) Query: 27 SIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGITQSLGWE 86 I+YS +Y D+ +EYRHVMLPK + K++P + +++E EWR LG+ QS GW Sbjct: 5 QIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTH---------LMSESEWRNLGVQQSQGWV 55 Query: 87 HYECHAPEPHILLFKRPL 104 HY H PEPHILLF+RPL Sbjct: 56 HYMIHEPEPHILLFRRPL 73 >4Y72-C nrPDB Length = 84 Score = 101 bits (252), Expect = 1e-22, Method: Composition-based stats. Identities = 41/78 (52%), Positives = 55/78 (70%), Gaps = 9/78 (11%) Query: 27 SIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGITQSLGWE 86 I+YS +Y D++YEYRHVMLP+ + K +P + +++E+EWR LG+ QSLGW Sbjct: 10 QIYYSDKYFDEHYEYRHVMLPRELSKQVPKTH---------LMSEEEWRRLGVQQSLGWV 60 Query: 87 HYECHAPEPHILLFKRPL 104 HY H PEPHILLF+RPL Sbjct: 61 HYMIHEPEPHILLFRRPL 78 >4QIW-A nrPDB Length = 906 Score = 29.0 bits (63), Expect = 0.92, Method: Composition-based stats. Identities = 15/56 (26%), Positives = 27/56 (48%), Gaps = 4/56 (7%) Query: 27 SIHYSPRYSDDNYEYRHVMLP---KAMLKVIPSDYFNSEVGTLRILTEDEWRGLGI 79 +I++ R ++ EY+H M+P + L+ IP D +G + EW L + Sbjct: 163 TIYWELRKDEEGNEYKHRMMPSEVRDRLEKIP-DKDLPLLGLHPEKSRPEWMVLTV 217 >5C6Q-A nrPDB Length = 209 Score = 28.6 bits (62), Expect = 1.2, Method: Composition-based stats. Identities = 20/64 (31%), Positives = 28/64 (43%), Gaps = 3/64 (4%) Query: 33 RYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGITQSLGWEHYECHA 92 +Y D R +ML + K+I ++ E L R L I QSL W+H C Sbjct: 132 KYGDTK-SARSIML-IELKKLIEANPLFREKLVFPTLKASRLRTL-INQSLNWQHQLCKN 188 Query: 93 PEPH 96 P P+ Sbjct: 189 PRPN 192 >3QQC-A nrPDB Length = 436 Score = 28.2 bits (61), Expect = 1.3, Method: Composition-based stats. Identities = 15/56 (26%), Positives = 26/56 (46%), Gaps = 4/56 (7%) Query: 27 SIHYSPRYSDDNYEYRHVMLP---KAMLKVIPSDYFNSEVGTLRILTEDEWRGLGI 79 +I++ R + EYRH ++P + L+ IP D +G + EW L + Sbjct: 224 TIYWEIRKDEQGNEYRHRLMPTEVRDWLEKIP-DKDLPLLGLDPEKSRPEWMVLTV 278 >6APH-A nrPDB Length = 445 Score = 28.2 bits (61), Expect = 1.4, Method: Composition-based stats. Identities = 11/44 (25%), Positives = 23/44 (52%) Query: 37 DNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGIT 80 D YE + LPK + + + + + L L+E++ + +G+T Sbjct: 390 DKYENKVYTLPKHLDEKVAALHLKKLGVELETLSEEQAKYIGVT 433 >2M1M-A nrPDB Length = 107 Score = 27.8 bits (60), Expect = 1.8, Method: Composition-based stats. Identities = 21/67 (31%), Positives = 32/67 (47%), Gaps = 8/67 (11%) Query: 12 KLTDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTE 71 KLT E + ++ S Y+P Y D + ++ ML +P D F + LRI+ Sbjct: 43 KLTIGEYSEREGYKGS-EYAPTYEDKDGDW---MLVGD----VPWDMFVTSCKRLRIMKG 94 Query: 72 DEWRGLG 78 E +GLG Sbjct: 95 TEAKGLG 101 >3ODT-A nrPDB Length = 313 Score = 27.4 bits (59), Expect = 2.4, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Query: 61 SEVGTLRILT-EDEWRGLGITQSLGWEHYECHAPEPHILLF 100 S GT+R+ + +D+W G + G+ + C+ E +LLF Sbjct: 36 SRDGTVRLWSKDDQWLGTVVYTGQGFLNSVCYDSEKELLLF 76 >3RQ4-A nrPDB Length = 247 Score = 26.3 bits (56), Expect = 5.4, Method: Composition-based stats. Identities = 8/37 (21%), Positives = 18/37 (48%) Query: 39 YEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWR 75 Y Y LP++ ++P ++ E +I++ W+ Sbjct: 93 YRYLRAFLPESGFTILPCTRYSMETNGAKIVSTRAWK 129 >3N58-A nrPDB Length = 464 Score = 26.3 bits (56), Expect = 5.6, Method: Composition-based stats. Identities = 10/44 (22%), Positives = 23/44 (52%) Query: 37 DNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLGIT 80 D Y+ +LPK + + + + + L +L+E++ +G+T Sbjct: 409 DAYKNEVYVLPKHLDEKVARLHLDKLGAKLTVLSEEQAAYIGVT 452 >5LUG-A nrPDB Length = 222 Score = 26.3 bits (56), Expect = 6.0, Method: Composition-based stats. Identities = 13/60 (21%), Positives = 32/60 (53%), Gaps = 5/60 (8%) Query: 70 TEDEWRGLGITQS---LGWEHYECHAPEPHILLFKRPLNY-EAELRAATAAAQQQQQQQQ 125 ++DEWRG+ + Q+ W Y + +P + +++ +Y E +LR +++ +++ Sbjct: 100 SKDEWRGMVLAQAPIMKAW-FYITYEKDPVLYMYQLLDDYKEGDLRIMPESSESPPTERE 158 >5HUQ-A nrPDB Length = 433 Score = 25.5 bits (54), Expect = 9.1, Method: Composition-based stats. Identities = 18/74 (24%), Positives = 25/74 (33%), Gaps = 3/74 (4%) Query: 14 TDQERARVLEFQDSIHYSPRYSDDNYEYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDE 73 T Q AR+L I S D H+ L K + + + Y + D Sbjct: 359 TAQIFARILVHHHVIFVSDLVDPDLITNMHMELAKTLDEAMEKAYAREGQAAKVTVIPD- 417 Query: 74 WRGLGITQSLGWEH 87 GLG+ W H Sbjct: 418 --GLGVIVKASWSH 429 >5HW4-A nrPDB Length = 248 Score = 25.5 bits (54), Expect = 9.5, Method: Composition-based stats. Identities = 11/39 (28%), Positives = 18/39 (46%) Query: 40 EYRHVMLPKAMLKVIPSDYFNSEVGTLRILTEDEWRGLG 78 E R+V+L + + K + + L + EDE R G Sbjct: 178 ESRYVVLARELTKTWETIHGAPVGELLAWVKEDENRRKG 216 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.126 0.374 Lambda K H 0.267 0.0384 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,994,674 Number of Sequences: 36641 Number of extensions: 223243 Number of successful extensions: 1656 Number of sequences better than 10.0: 44 Number of HSP's better than 10.0 without gapping: 59 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 1057 Number of HSP's gapped (non-prelim): 206 length of query: 150 length of database: 10,336,785 effective HSP length: 88 effective length of query: 62 effective length of database: 7,112,377 effective search space: 440967374 effective search space used: 440967374 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 54 (25.5 bits)