BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= P20436
(146 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
5C3E-H nrPDB 301 6e-83
5X4Z-H nrPDB 184 1e-47
3H0G-H nrPDB 99 9e-22
6GML-H nrPDB 87 3e-18
4ADZ-A nrPDB 29 0.91
3MDY-A nrPDB 28 1.1
5SZI-B nrPDB 28 1.6
5CJH-A nrPDB 27 3.1
3PUG-A nrPDB 26 5.3
3V6B-R nrPDB 26 5.7
5OYJ-C nrPDB 26 5.8
5OHS-A nrPDB 26 7.5
3O0H-A nrPDB 26 8.1
3V2A-R nrPDB 25 8.7
>5C3E-H nrPDB
Length = 146
Score = 301 bits (772), Expect = 6e-83, Method: Compositional matrix adjust.
Identities = 146/146 (100%), Positives = 146/146 (100%)
Query: 1 MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA 60
MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA
Sbjct: 1 MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA 60
Query: 61 SSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGG 120
SSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGG
Sbjct: 61 SSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGG 120
Query: 121 LLMRLEGNYRNLNNLKQENAYLLIRR 146
LLMRLEGNYRNLNNLKQENAYLLIRR
Sbjct: 121 LLMRLEGNYRNLNNLKQENAYLLIRR 146
>5X4Z-H nrPDB
Length = 145
Score = 184 bits (468), Expect = 1e-47, Method: Compositional matrix adjust.
Identities = 96/148 (64%), Positives = 112/148 (75%), Gaps = 5/148 (3%)
Query: 1 MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA 60
MS+ LFDDIF V VD GRYNKV RI STT KLTLDIN E+FPV+ DSLTVT+A
Sbjct: 1 MSSALFDDIFTVQTVDNGRYNKVSRIIGISTTNSAIKLTLDINNEMFPVSQDDSLTVTLA 60
Query: 61 SSLNL--EDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSF 118
+SL+L ED AN S +SWRPP+ D+SLADDYDYVM+GT YKFEE +D I VY SF
Sbjct: 61 NSLSLDGEDESANFS---KSWRPPKPTDKSLADDYDYVMFGTVYKFEEGDEDKIKVYVSF 117
Query: 119 GGLLMRLEGNYRNLNNLKQENAYLLIRR 146
GGLLM LEG Y++L +LKQ+N Y+LIRR
Sbjct: 118 GGLLMCLEGGYKSLASLKQDNLYILIRR 145
>3H0G-H nrPDB
Length = 125
Score = 98.6 bits (244), Expect = 9e-22, Method: Compositional matrix adjust.
Identities = 54/144 (37%), Positives = 80/144 (55%), Gaps = 22/144 (15%)
Query: 3 NTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIASS 62
+ L D+IF V+ VD +Y +V RI A S Q+ LTLDIN +++P+ + ++ I S+
Sbjct: 4 SVLLDEIFTVTSVDKQKYQRVSRITAVSG-QNDMNLTLDINSQIYPLEKDATFSLQITSN 62
Query: 63 LNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLL 122
LN D L + DY+MYG Y+ EE + ++VY SFGGLL
Sbjct: 63 LNSPD---------------------LKEAADYIMYGKVYRVEEAKDEKVSVYVSFGGLL 101
Query: 123 MRLEGNYRNLNNLKQENAYLLIRR 146
M +EG++R L L ++ YLL+RR
Sbjct: 102 MAIEGSHRKLYRLSLDHVYLLLRR 125
>6GML-H nrPDB
Length = 150
Score = 86.7 bits (213), Expect = 3e-18, Method: Compositional matrix adjust.
Identities = 54/154 (35%), Positives = 90/154 (58%), Gaps = 15/154 (9%)
Query: 1 MSNTLFDDIFQVSEVDP--GRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVT 58
M+ LF+DIF V ++DP ++++V R+ S + + L LD+N++++PV D +
Sbjct: 1 MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESF-KMDLILDVNIQIYPVDLGDKFRLV 59
Query: 59 IASSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFE--EVSKDL---IA 113
IAS+L ED +D + P S AD ++YVMYG Y+ E E S + ++
Sbjct: 60 IASTL-YEDGTLDDGEYNPTDDRP-----SRADQFEYVMYGKVYRIEGDETSTEAATRLS 113
Query: 114 VYYSFGGLLMRLEGNYRNLNNLKQEN-AYLLIRR 146
Y S+GGLLMRL+G+ NL+ + ++ YLL+++
Sbjct: 114 AYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKK 147
>4ADZ-A nrPDB
Length = 136
Score = 28.9 bits (63), Expect = 0.91, Method: Compositional matrix adjust.
Identities = 18/77 (23%), Positives = 33/77 (42%), Gaps = 13/77 (16%)
Query: 68 TPANDSSATRSWRPPQAGDRSLADDYDYVMYG-TAYKFEEVSKDLIAVYYSFGGLLMRLE 126
+PA D + ++ R +A + D+D ++G K E + + L R+E
Sbjct: 15 SPAVDGAVNQTARQAEADGTDIVTDHDRGVHGYHKQKAEHLKR------------LRRIE 62
Query: 127 GNYRNLNNLKQENAYLL 143
G R L + E+ Y +
Sbjct: 63 GQIRGLQRMVDEDVYCI 79
>3MDY-A nrPDB
Length = 337
Score = 28.5 bits (62), Expect = 1.1, Method: Compositional matrix adjust.
Identities = 22/84 (26%), Positives = 36/84 (42%), Gaps = 10/84 (11%)
Query: 54 SLTVTIASSLNLEDTPANDSSATRSWRPPQAGDRSLADDY--DYV---MYGTAYKFEEVS 108
L V S N D P N T+ + PP+ D SL ++ Y+ MY EV+
Sbjct: 187 GLAVKFISDTNEVDIPPNTRVGTKRYMPPEVLDESLNRNHFQSYIMADMYSFGLILWEVA 246
Query: 109 KDLIAVYYSFGGLLMRLEGNYRNL 132
+ ++ GG++ + Y +L
Sbjct: 247 RRCVS-----GGIVEEYQLPYHDL 265
>5SZI-B nrPDB
Length = 153
Score = 27.7 bits (60), Expect = 1.6, Method: Compositional matrix adjust.
Identities = 24/85 (28%), Positives = 38/85 (44%), Gaps = 6/85 (7%)
Query: 13 SEVDPGRYNKVCRIEAASTTQDQCKLTLD---INVELFPVAAQDSLTVTIASSLNLEDTP 69
SE+ R K R EA S TQD+ +L + + +E + +S + +A L LED
Sbjct: 31 SEIQGVRLEKALRGEADSGTQDEAQLLQEWFKLVLEKNKLMRYESELLIMAQELELED-- 88
Query: 70 ANDSSATRSWRPPQAGDRSLADDYD 94
+ S + R + S D+ D
Sbjct: 89 -HQSRLEQKLREKMLKEESQKDEKD 112
>5CJH-A nrPDB
Length = 764
Score = 26.9 bits (58), Expect = 3.1, Method: Compositional matrix adjust.
Identities = 19/72 (26%), Positives = 33/72 (45%), Gaps = 18/72 (25%)
Query: 77 RSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAV-----------YYSFGGLLMRL 125
R ++P Q L D+DY + +E V KD+ A+ + ++GGL +R+
Sbjct: 60 RQFQPSQ---NPLGGDFDYAEAFQSLDYEAVKKDIAALMTESQDWWPADFGNYGGLFVRM 116
Query: 126 ----EGNYRNLN 133
G YR ++
Sbjct: 117 AWHSAGTYRAMD 128
>3PUG-A nrPDB
Length = 433
Score = 26.2 bits (56), Expect = 5.3, Method: Compositional matrix adjust.
Identities = 20/73 (27%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
Query: 68 TPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLLMRLEG 127
TP A S PP+ G L D + V + E D +++ + GG +R+ G
Sbjct: 260 TPGQVVEANTSPLPPKTGSWLLDADGEEVELASEDGVEAYDGDRLSLEATDGGYELRVGG 319
Query: 128 NYRNL--NNLKQE 138
+ R L + L++E
Sbjct: 320 DARELTADELREE 332
>3V6B-R nrPDB
Length = 424
Score = 26.2 bits (56), Expect = 5.7, Method: Compositional matrix adjust.
Identities = 10/20 (50%), Positives = 12/20 (60%)
Query: 81 PPQAGDRSLADDYDYVMYGT 100
PPQ G++SL D YGT
Sbjct: 290 PPQIGEKSLISPVDSYQYGT 309
>5OYJ-C nrPDB
Length = 234
Score = 26.2 bits (56), Expect = 5.8, Method: Compositional matrix adjust.
Identities = 10/20 (50%), Positives = 12/20 (60%)
Query: 81 PPQAGDRSLADDYDYVMYGT 100
PPQ G++SL D YGT
Sbjct: 99 PPQIGEKSLISPVDSYQYGT 118
>5OHS-A nrPDB
Length = 672
Score = 25.8 bits (55), Expect = 7.5, Method: Composition-based stats.
Identities = 28/99 (28%), Positives = 44/99 (44%), Gaps = 7/99 (7%)
Query: 52 QDSLTVTIASSLNLEDTPANDSSATRSWRPPQAGD--RSLADDYDYVMYGTAYKFEEVSK 109
+D T+ I + + L +P D A + + D R D DYV+ TA + EVS
Sbjct: 7 KDGFTIAIGNRIILSHSP--DKPAFFAGFGEERMDMYRGNFDIEDYVIERTALRHAEVSG 64
Query: 110 DLIAVYYSFGG---LLMRLEGNYRNLNNLKQENAYLLIR 145
D + + + G L + L+GN L L + L +R
Sbjct: 65 DSVTLSSAPGQAPRLRLTLDGNAIRLTALDETINRLWLR 103
>3O0H-A nrPDB
Length = 484
Score = 25.8 bits (55), Expect = 8.1, Method: Composition-based stats.
Identities = 13/50 (26%), Positives = 24/50 (48%)
Query: 43 NVELFPVAAQDSLTVTIASSLNLEDTPANDSSATRSWRPPQAGDRSLADD 92
+++L PVA D++ + N TP D T + P+ G L+++
Sbjct: 330 HIQLTPVAIHDAMCFVKNAFENTSTTPDYDLITTAVFSQPEIGTVGLSEE 379
>3V2A-R nrPDB
Length = 772
Score = 25.4 bits (54), Expect = 8.7, Method: Composition-based stats.
Identities = 10/20 (50%), Positives = 12/20 (60%)
Query: 81 PPQAGDRSLADDYDYVMYGT 100
PPQ G++SL D YGT
Sbjct: 420 PPQIGEKSLISPVDSYQYGT 439
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
5C3E-H nrPDB 219 3e-58
5X4Z-H nrPDB 198 6e-52
6GML-H nrPDB 166 4e-42
3H0G-H nrPDB 158 9e-40
Sequences not found previously or not previously below threshold:
3PUG-A nrPDB 29 0.77
3O3M-A nrPDB 28 1.3
1XTZ-A nrPDB 27 2.3
1YAK-A nrPDB 27 3.5
5X06-E nrPDB 25 8.6
CONVERGED!
>5C3E-H nrPDB
Length = 146
Score = 219 bits (558), Expect = 3e-58, Method: Composition-based stats.
Identities = 146/146 (100%), Positives = 146/146 (100%)
Query: 1 MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA 60
MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA
Sbjct: 1 MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA 60
Query: 61 SSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGG 120
SSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGG
Sbjct: 61 SSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGG 120
Query: 121 LLMRLEGNYRNLNNLKQENAYLLIRR 146
LLMRLEGNYRNLNNLKQENAYLLIRR
Sbjct: 121 LLMRLEGNYRNLNNLKQENAYLLIRR 146
>5X4Z-H nrPDB
Length = 145
Score = 198 bits (504), Expect = 6e-52, Method: Composition-based stats.
Identities = 96/148 (64%), Positives = 112/148 (75%), Gaps = 5/148 (3%)
Query: 1 MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIA 60
MS+ LFDDIF V VD GRYNKV RI STT KLTLDIN E+FPV+ DSLTVT+A
Sbjct: 1 MSSALFDDIFTVQTVDNGRYNKVSRIIGISTTNSAIKLTLDINNEMFPVSQDDSLTVTLA 60
Query: 61 SSLNL--EDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSF 118
+SL+L ED AN S +SWRPP+ D+SLADDYDYVM+GT YKFEE +D I VY SF
Sbjct: 61 NSLSLDGEDESANFS---KSWRPPKPTDKSLADDYDYVMFGTVYKFEEGDEDKIKVYVSF 117
Query: 119 GGLLMRLEGNYRNLNNLKQENAYLLIRR 146
GGLLM LEG Y++L +LKQ+N Y+LIRR
Sbjct: 118 GGLLMCLEGGYKSLASLKQDNLYILIRR 145
>6GML-H nrPDB
Length = 150
Score = 166 bits (420), Expect = 4e-42, Method: Composition-based stats.
Identities = 54/154 (35%), Positives = 90/154 (58%), Gaps = 15/154 (9%)
Query: 1 MSNTLFDDIFQVSEVDP--GRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVT 58
M+ LF+DIF V ++DP ++++V R+ S + + L LD+N++++PV D +
Sbjct: 1 MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESF-KMDLILDVNIQIYPVDLGDKFRLV 59
Query: 59 IASSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFE--EVSKDL---IA 113
IAS+L ED +D + P S AD ++YVMYG Y+ E E S + ++
Sbjct: 60 IASTL-YEDGTLDDGEYNPTDDRP-----SRADQFEYVMYGKVYRIEGDETSTEAATRLS 113
Query: 114 VYYSFGGLLMRLEGNYRNLNNLKQEN-AYLLIRR 146
Y S+GGLLMRL+G+ NL+ + ++ YLL+++
Sbjct: 114 AYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKK 147
>3H0G-H nrPDB
Length = 125
Score = 158 bits (399), Expect = 9e-40, Method: Composition-based stats.
Identities = 54/144 (37%), Positives = 80/144 (55%), Gaps = 22/144 (15%)
Query: 3 NTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIASS 62
+ L D+IF V+ VD +Y +V RI A S Q+ LTLDIN +++P+ + ++ I S+
Sbjct: 4 SVLLDEIFTVTSVDKQKYQRVSRITAVSG-QNDMNLTLDINSQIYPLEKDATFSLQITSN 62
Query: 63 LNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLL 122
LN D L + DY+MYG Y+ EE + ++VY SFGGLL
Sbjct: 63 LNSPD---------------------LKEAADYIMYGKVYRVEEAKDEKVSVYVSFGGLL 101
Query: 123 MRLEGNYRNLNNLKQENAYLLIRR 146
M +EG++R L L ++ YLL+RR
Sbjct: 102 MAIEGSHRKLYRLSLDHVYLLLRR 125
>3PUG-A nrPDB
Length = 433
Score = 28.9 bits (63), Expect = 0.77, Method: Composition-based stats.
Identities = 18/65 (27%), Positives = 28/65 (43%)
Query: 68 TPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLLMRLEG 127
TP A S PP+ G L D + V + E D +++ + GG +R+ G
Sbjct: 260 TPGQVVEANTSPLPPKTGSWLLDADGEEVELASEDGVEAYDGDRLSLEATDGGYELRVGG 319
Query: 128 NYRNL 132
+ R L
Sbjct: 320 DAREL 324
>3O3M-A nrPDB
Length = 408
Score = 28.2 bits (61), Expect = 1.3, Method: Composition-based stats.
Identities = 10/49 (20%), Positives = 22/49 (44%)
Query: 8 DIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLT 56
+I + DP ++ +V +I A + + ++L + P+ D T
Sbjct: 168 EIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFT 216
>1XTZ-A nrPDB
Length = 264
Score = 27.4 bits (59), Expect = 2.3, Method: Composition-based stats.
Identities = 8/36 (22%), Positives = 12/36 (33%)
Query: 16 DPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAA 51
DP Y + T L LD ++L +
Sbjct: 63 DPKFYEVASKFICIPTGFQSRNLILDNKLQLGSIEQ 98
>1YAK-A nrPDB
Length = 263
Score = 26.6 bits (57), Expect = 3.5, Method: Composition-based stats.
Identities = 15/62 (24%), Positives = 24/62 (38%), Gaps = 2/62 (3%)
Query: 52 QDSLTVTIASSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDL 111
D + +N D A +S T R + ++ Y+Y +G AY+ E S
Sbjct: 192 GDWFRQQVEEQINRFDELAENS--TEEVRAKMKENFVISSYYEYQFWGMAYRKEGWSDSA 249
Query: 112 IA 113
I
Sbjct: 250 IK 251
>5X06-E nrPDB
Length = 253
Score = 25.5 bits (54), Expect = 8.6, Method: Composition-based stats.
Identities = 9/32 (28%), Positives = 16/32 (50%)
Query: 79 WRPPQAGDRSLADDYDYVMYGTAYKFEEVSKD 110
RPP+ + L D + +G YK + +S +
Sbjct: 157 DRPPRQLNLGLPDLASRLDWGQIYKLQPLSDE 188
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.311 0.128 0.315
Lambda K H
0.267 0.0390 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,760,620
Number of Sequences: 36641
Number of extensions: 151363
Number of successful extensions: 406
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 375
Number of HSP's gapped (non-prelim): 24
length of query: 146
length of database: 10,336,785
effective HSP length: 88
effective length of query: 58
effective length of database: 7,112,377
effective search space: 412517866
effective search space used: 412517866
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.4 bits)
S2: 54 (25.5 bits)