BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= P20435
(155 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
5U5Q-F nrPDB 319 4e-88
6A5U-F nrPDB 178 1e-45
5IP9-F nrPDB 178 1e-45
5U0S-f nrPDB 153 2e-38
6EXV-F nrPDB 132 9e-32
2WB1-I nrPDB 54 3e-08
3HI0-A nrPDB 29 1.1
4GKB-A nrPDB 28 1.1
6IGZ-B nrPDB 28 1.2
3SGZ-A nrPDB 28 2.1
3IHM-A nrPDB 28 2.4
2LAA-A nrPDB 27 5.1
1K3I-A nrPDB 26 8.0
5HHK-A nrPDB 26 8.2
>5U5Q-F nrPDB
Length = 155
Score = 319 bits (817), Expect = 4e-88, Method: Compositional matrix adjust.
Identities = 155/155 (100%), Positives = 155/155 (100%)
Query: 1 MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ 60
MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ
Sbjct: 1 MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ 60
Query: 61 HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI 120
HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI
Sbjct: 61 HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI 120
Query: 121 AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL 155
AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL
Sbjct: 121 AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL 155
>6A5U-F nrPDB
Length = 155
Score = 178 bits (451), Expect = 1e-45, Method: Compositional matrix adjust.
Identities = 91/151 (60%), Positives = 115/151 (76%), Gaps = 1/151 (0%)
Query: 5 EEAFNDGNENFEDFDVEHFSDEETYEEKPQFKD-GETTDANGKTIVTGGNGPEDFQQHEQ 63
+EAFN+ ENFE+F+ EHFSD+ + Q +D A+G+ I+ G E +
Sbjct: 4 DEAFNEQTENFENFEDEHFSDDNFEDRSTQPEDYAVGVTADGRQIINGDGIQEVNGTIKA 63
Query: 64 IRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMK 123
R+++ KE AI K++R TTPY+TKYERARILGTRALQISMNAPV VD+EGETDPL+IAMK
Sbjct: 64 HRKRSNKELAILKEERTTTPYLTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMK 123
Query: 124 ELAEKKIPLVIRRYLPDGSFEDWSVEELIVD 154
EL+++KIPLVIRRYLPDGS+EDW +ELIVD
Sbjct: 124 ELSQRKIPLVIRRYLPDGSYEDWGCDELIVD 154
>5IP9-F nrPDB
Length = 87
Score = 178 bits (451), Expect = 1e-45, Method: Compositional matrix adjust.
Identities = 87/87 (100%), Positives = 87/87 (100%)
Query: 69 LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK 128
LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK
Sbjct: 1 LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK 60
Query: 129 KIPLVIRRYLPDGSFEDWSVEELIVDL 155
KIPLVIRRYLPDGSFEDWSVEELIVDL
Sbjct: 61 KIPLVIRRYLPDGSFEDWSVEELIVDL 87
>5U0S-f nrPDB
Length = 142
Score = 153 bits (387), Expect = 2e-38, Method: Compositional matrix adjust.
Identities = 89/155 (57%), Positives = 104/155 (67%), Gaps = 16/155 (10%)
Query: 1 MSDYEE--AFN-DGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPED 57
MSDYEE AF DG E+ D DE ++ GE + T++T ED
Sbjct: 1 MSDYEEDEAFGMDGAVMEEEVDELEMIDENGQSQQGVSHPGEPS----TTVIT-----ED 51
Query: 58 FQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDP 117
+ + KA+ K+ R TTPYMTKYERARILGTRALQISMNAPV VDLEGETDP
Sbjct: 52 VASSKTAQSG----KAVAKEDRTTTPYMTKYERARILGTRALQISMNAPVLVDLEGETDP 107
Query: 118 LRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELI 152
L+IAMKELA+KKIPL++RRYLPDGS+EDWSV ELI
Sbjct: 108 LQIAMKELAQKKIPLLVRRYLPDGSYEDWSVAELI 142
>6EXV-F nrPDB
Length = 127
Score = 132 bits (331), Expect = 9e-32, Method: Compositional matrix adjust.
Identities = 59/76 (77%), Positives = 69/76 (90%)
Query: 78 QRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKIPLVIRRY 137
+R TTPYMTKYERAR+LGTRALQI+M APV V+LEGETDPL IAMKEL +KIP++IRRY
Sbjct: 50 KRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRY 109
Query: 138 LPDGSFEDWSVEELIV 153
LPDGS+EDW V+ELI+
Sbjct: 110 LPDGSYEDWGVDELII 125
>2WB1-I nrPDB
Length = 95
Score = 53.5 bits (127), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 25/59 (42%), Positives = 38/59 (64%), Gaps = 2/59 (3%)
Query: 85 MTKYERARILGTRALQISMNAPVFVDLE--GETDPLRIAMKELAEKKIPLVIRRYLPDG 141
+T+YE AR++ RALQ++M AP +D+ TD + IA +E +P+ IRR LP+G
Sbjct: 27 LTRYEIARVISARALQLAMGAPALIDINNLSSTDVISIAEEEFRRGVLPITIRRRLPNG 85
>3HI0-A nrPDB
Length = 508
Score = 28.9 bits (63), Expect = 1.1, Method: Composition-based stats.
Identities = 22/87 (25%), Positives = 41/87 (47%), Gaps = 17/87 (19%)
Query: 72 KAIPKDQRATTPYMTKYERARILGTRALQISMNA---------PVFVDLEGETDPLRIAM 122
+A+ K++R+ P+ R + + +I+ +A + + E E+DPL +A
Sbjct: 263 QAVSKNRRSLLPFGAIAMREVLRAMKPAKIAFSAQGVREGYLYSLLTEAERESDPLLVAA 322
Query: 123 KELAEKKIPLVIRRYLPDGSFE--DWS 147
ELA ++R P+ + E DWS
Sbjct: 323 DELA------ILRARSPEHARELADWS 343
>4GKB-A nrPDB
Length = 258
Score = 28.5 bits (62), Expect = 1.1, Method: Compositional matrix adjust.
Identities = 11/24 (45%), Positives = 16/24 (66%)
Query: 122 MKELAEKKIPLVIRRYLPDGSFED 145
M+ E+ IP+V R+ PDG+F D
Sbjct: 25 MRLAEERAIPVVFARHAPDGAFLD 48
>6IGZ-B nrPDB
Length = 734
Score = 28.5 bits (62), Expect = 1.2, Method: Composition-based stats.
Identities = 13/40 (32%), Positives = 19/40 (47%)
Query: 33 PQFKDGETTDANGKTIVTGGNGPEDFQQHEQIRRKTLKEK 72
P+F G D + I G DF+ H+ I +TL +K
Sbjct: 6 PKFSQGLAQDPTTRRIWFGVATAHDFESHDSITEETLYQK 45
>3SGZ-A nrPDB
Length = 352
Score = 27.7 bits (60), Expect = 2.1, Method: Compositional matrix adjust.
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 8/63 (12%)
Query: 89 ERARILGTRALQISMNAPVFVD--------LEGETDPLRIAMKELAEKKIPLVIRRYLPD 140
+RA LG +AL I+++ PV + L E + L+ A++ L E+K + P
Sbjct: 142 QRAEALGFKALVITIDTPVLGNRRRDKRNQLNLEANILKAALRALKEEKPTQSVPVLFPK 201
Query: 141 GSF 143
SF
Sbjct: 202 ASF 204
>3IHM-A nrPDB
Length = 430
Score = 27.7 bits (60), Expect = 2.4, Method: Compositional matrix adjust.
Identities = 11/21 (52%), Positives = 14/21 (66%)
Query: 33 PQFKDGETTDANGKTIVTGGN 53
P F+DG T NGKTI+ G+
Sbjct: 295 PAFRDGHATLNNGKTIIGLGD 315
>2LAA-A nrPDB
Length = 104
Score = 26.6 bits (57), Expect = 5.1, Method: Compositional matrix adjust.
Identities = 10/24 (41%), Positives = 16/24 (66%)
Query: 1 MSDYEEAFNDGNENFEDFDVEHFS 24
S E AFNDGN N++ + +++S
Sbjct: 55 ASQLEAAFNDGNNNWDSNNTKNYS 78
>1K3I-A nrPDB
Length = 656
Score = 25.8 bits (55), Expect = 8.0, Method: Composition-based stats.
Identities = 11/26 (42%), Positives = 15/26 (57%)
Query: 28 TYEEKPQFKDGETTDANGKTIVTGGN 53
T + F G + D NG+ +VTGGN
Sbjct: 237 TVTKHDMFCPGISMDGNGQIVVTGGN 262
>5HHK-A nrPDB
Length = 305
Score = 25.8 bits (55), Expect = 8.2, Method: Compositional matrix adjust.
Identities = 15/51 (29%), Positives = 24/51 (47%), Gaps = 5/51 (9%)
Query: 6 EAFNDGNENFEDFDVEHFSDEETYEE-----KPQFKDGETTDANGKTIVTG 51
+ NDGN+ D D+E F D +++ KDG+ K +V+G
Sbjct: 140 DMMNDGNDWIVDIDLEKFFDTVNHDKLMTIIGRTIKDGDVISIVRKYLVSG 190
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
5U5Q-F nrPDB 233 2e-62
6A5U-F nrPDB 221 1e-58
5U0S-f nrPDB 176 4e-45
5IP9-F nrPDB 148 8e-37
6EXV-F nrPDB 130 2e-31
2WB1-I nrPDB 89 8e-19
Sequences not found previously or not previously below threshold:
3SGZ-A nrPDB 28 1.9
5ITV-A nrPDB 27 2.7
4GKB-A nrPDB 26 5.4
CONVERGED!
>5U5Q-F nrPDB
Length = 155
Score = 233 bits (595), Expect = 2e-62, Method: Composition-based stats.
Identities = 155/155 (100%), Positives = 155/155 (100%)
Query: 1 MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ 60
MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ
Sbjct: 1 MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ 60
Query: 61 HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI 120
HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI
Sbjct: 61 HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI 120
Query: 121 AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL 155
AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL
Sbjct: 121 AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL 155
>6A5U-F nrPDB
Length = 155
Score = 221 bits (562), Expect = 1e-58, Method: Composition-based stats.
Identities = 91/151 (60%), Positives = 115/151 (76%), Gaps = 1/151 (0%)
Query: 5 EEAFNDGNENFEDFDVEHFSDEETYEEKPQFKD-GETTDANGKTIVTGGNGPEDFQQHEQ 63
+EAFN+ ENFE+F+ EHFSD+ + Q +D A+G+ I+ G E +
Sbjct: 4 DEAFNEQTENFENFEDEHFSDDNFEDRSTQPEDYAVGVTADGRQIINGDGIQEVNGTIKA 63
Query: 64 IRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMK 123
R+++ KE AI K++R TTPY+TKYERARILGTRALQISMNAPV VD+EGETDPL+IAMK
Sbjct: 64 HRKRSNKELAILKEERTTTPYLTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMK 123
Query: 124 ELAEKKIPLVIRRYLPDGSFEDWSVEELIVD 154
EL+++KIPLVIRRYLPDGS+EDW +ELIVD
Sbjct: 124 ELSQRKIPLVIRRYLPDGSYEDWGCDELIVD 154
>5U0S-f nrPDB
Length = 142
Score = 176 bits (445), Expect = 4e-45, Method: Composition-based stats.
Identities = 89/155 (57%), Positives = 104/155 (67%), Gaps = 16/155 (10%)
Query: 1 MSDYEE--AFN-DGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPED 57
MSDYEE AF DG E+ D DE ++ GE + T++T ED
Sbjct: 1 MSDYEEDEAFGMDGAVMEEEVDELEMIDENGQSQQGVSHPGEPST----TVIT-----ED 51
Query: 58 FQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDP 117
+ + KA+ K+ R TTPYMTKYERARILGTRALQISMNAPV VDLEGETDP
Sbjct: 52 VASSKTAQSG----KAVAKEDRTTTPYMTKYERARILGTRALQISMNAPVLVDLEGETDP 107
Query: 118 LRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELI 152
L+IAMKELA+KKIPL++RRYLPDGS+EDWSV ELI
Sbjct: 108 LQIAMKELAQKKIPLLVRRYLPDGSYEDWSVAELI 142
>5IP9-F nrPDB
Length = 87
Score = 148 bits (374), Expect = 8e-37, Method: Composition-based stats.
Identities = 87/87 (100%), Positives = 87/87 (100%)
Query: 69 LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK 128
LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK
Sbjct: 1 LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK 60
Query: 129 KIPLVIRRYLPDGSFEDWSVEELIVDL 155
KIPLVIRRYLPDGSFEDWSVEELIVDL
Sbjct: 61 KIPLVIRRYLPDGSFEDWSVEELIVDL 87
>6EXV-F nrPDB
Length = 127
Score = 130 bits (327), Expect = 2e-31, Method: Composition-based stats.
Identities = 60/83 (72%), Positives = 71/83 (85%)
Query: 71 EKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKI 130
E+ +R TTPYMTKYERAR+LGTRALQI+M APV V+LEGETDPL IAMKEL +KI
Sbjct: 43 ERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKI 102
Query: 131 PLVIRRYLPDGSFEDWSVEELIV 153
P++IRRYLPDGS+EDW V+ELI+
Sbjct: 103 PIIIRRYLPDGSYEDWGVDELII 125
>2WB1-I nrPDB
Length = 95
Score = 89.0 bits (219), Expect = 8e-19, Method: Composition-based stats.
Identities = 25/59 (42%), Positives = 38/59 (64%), Gaps = 2/59 (3%)
Query: 85 MTKYERARILGTRALQISMNAPVFVDLE--GETDPLRIAMKELAEKKIPLVIRRYLPDG 141
+T+YE AR++ RALQ++M AP +D+ TD + IA +E +P+ IRR LP+G
Sbjct: 27 LTRYEIARVISARALQLAMGAPALIDINNLSSTDVISIAEEEFRRGVLPITIRRRLPNG 85
>3SGZ-A nrPDB
Length = 352
Score = 27.8 bits (60), Expect = 1.9, Method: Composition-based stats.
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 8/63 (12%)
Query: 89 ERARILGTRALQISMNAPVFVD--------LEGETDPLRIAMKELAEKKIPLVIRRYLPD 140
+RA LG +AL I+++ PV + L E + L+ A++ L E+K + P
Sbjct: 142 QRAEALGFKALVITIDTPVLGNRRRDKRNQLNLEANILKAALRALKEEKPTQSVPVLFPK 201
Query: 141 GSF 143
SF
Sbjct: 202 ASF 204
>5ITV-A nrPDB
Length = 255
Score = 27.4 bits (59), Expect = 2.7, Method: Composition-based stats.
Identities = 12/67 (17%), Positives = 24/67 (35%), Gaps = 2/67 (2%)
Query: 1 MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETT--DANGKTIVTGGNGPEDF 58
++D +EA + E+ D HF + +E E+ G ++ G E
Sbjct: 36 VADIDEAQGEAMVRKENNDRLHFVQTDITDEAACQHAVESAVHTFGGLDVLINNAGIEIV 95
Query: 59 QQHEQIR 65
++
Sbjct: 96 APIHEME 102
>4GKB-A nrPDB
Length = 258
Score = 26.2 bits (56), Expect = 5.4, Method: Composition-based stats.
Identities = 11/24 (45%), Positives = 16/24 (66%)
Query: 122 MKELAEKKIPLVIRRYLPDGSFED 145
M+ E+ IP+V R+ PDG+F D
Sbjct: 25 MRLAEERAIPVVFARHAPDGAFLD 48
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.310 0.128 0.329
Lambda K H
0.267 0.0371 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,865,852
Number of Sequences: 36641
Number of extensions: 222043
Number of successful extensions: 527
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 497
Number of HSP's gapped (non-prelim): 40
length of query: 155
length of database: 10,336,785
effective HSP length: 89
effective length of query: 66
effective length of database: 7,075,736
effective search space: 466998576
effective search space used: 466998576
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 54 (25.6 bits)