BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P20435
         (155 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

5U5Q-F nrPDB                                                          319   4e-88
6A5U-F nrPDB                                                          178   1e-45
5IP9-F nrPDB                                                          178   1e-45
5U0S-f nrPDB                                                          153   2e-38
6EXV-F nrPDB                                                          132   9e-32
2WB1-I nrPDB                                                           54   3e-08
3HI0-A nrPDB                                                           29   1.1  
4GKB-A nrPDB                                                           28   1.1  
6IGZ-B nrPDB                                                           28   1.2  
3SGZ-A nrPDB                                                           28   2.1  
3IHM-A nrPDB                                                           28   2.4  
2LAA-A nrPDB                                                           27   5.1  
1K3I-A nrPDB                                                           26   8.0  
5HHK-A nrPDB                                                           26   8.2  

>5U5Q-F nrPDB
          Length = 155

 Score =  319 bits (817), Expect = 4e-88,   Method: Compositional matrix adjust.
 Identities = 155/155 (100%), Positives = 155/155 (100%)

Query: 1   MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ 60
           MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ
Sbjct: 1   MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ 60

Query: 61  HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI 120
           HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI
Sbjct: 61  HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI 120

Query: 121 AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL 155
           AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL
Sbjct: 121 AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL 155


>6A5U-F nrPDB
          Length = 155

 Score =  178 bits (451), Expect = 1e-45,   Method: Compositional matrix adjust.
 Identities = 91/151 (60%), Positives = 115/151 (76%), Gaps = 1/151 (0%)

Query: 5   EEAFNDGNENFEDFDVEHFSDEETYEEKPQFKD-GETTDANGKTIVTGGNGPEDFQQHEQ 63
           +EAFN+  ENFE+F+ EHFSD+   +   Q +D      A+G+ I+ G    E     + 
Sbjct: 4   DEAFNEQTENFENFEDEHFSDDNFEDRSTQPEDYAVGVTADGRQIINGDGIQEVNGTIKA 63

Query: 64  IRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMK 123
            R+++ KE AI K++R TTPY+TKYERARILGTRALQISMNAPV VD+EGETDPL+IAMK
Sbjct: 64  HRKRSNKELAILKEERTTTPYLTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMK 123

Query: 124 ELAEKKIPLVIRRYLPDGSFEDWSVEELIVD 154
           EL+++KIPLVIRRYLPDGS+EDW  +ELIVD
Sbjct: 124 ELSQRKIPLVIRRYLPDGSYEDWGCDELIVD 154


>5IP9-F nrPDB
          Length = 87

 Score =  178 bits (451), Expect = 1e-45,   Method: Compositional matrix adjust.
 Identities = 87/87 (100%), Positives = 87/87 (100%)

Query: 69  LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK 128
           LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK
Sbjct: 1   LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK 60

Query: 129 KIPLVIRRYLPDGSFEDWSVEELIVDL 155
           KIPLVIRRYLPDGSFEDWSVEELIVDL
Sbjct: 61  KIPLVIRRYLPDGSFEDWSVEELIVDL 87


>5U0S-f nrPDB
          Length = 142

 Score =  153 bits (387), Expect = 2e-38,   Method: Compositional matrix adjust.
 Identities = 89/155 (57%), Positives = 104/155 (67%), Gaps = 16/155 (10%)

Query: 1   MSDYEE--AFN-DGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPED 57
           MSDYEE  AF  DG    E+ D     DE    ++     GE +     T++T     ED
Sbjct: 1   MSDYEEDEAFGMDGAVMEEEVDELEMIDENGQSQQGVSHPGEPS----TTVIT-----ED 51

Query: 58  FQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDP 117
               +  +      KA+ K+ R TTPYMTKYERARILGTRALQISMNAPV VDLEGETDP
Sbjct: 52  VASSKTAQSG----KAVAKEDRTTTPYMTKYERARILGTRALQISMNAPVLVDLEGETDP 107

Query: 118 LRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELI 152
           L+IAMKELA+KKIPL++RRYLPDGS+EDWSV ELI
Sbjct: 108 LQIAMKELAQKKIPLLVRRYLPDGSYEDWSVAELI 142


>6EXV-F nrPDB
          Length = 127

 Score =  132 bits (331), Expect = 9e-32,   Method: Compositional matrix adjust.
 Identities = 59/76 (77%), Positives = 69/76 (90%)

Query: 78  QRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKIPLVIRRY 137
           +R TTPYMTKYERAR+LGTRALQI+M APV V+LEGETDPL IAMKEL  +KIP++IRRY
Sbjct: 50  KRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRY 109

Query: 138 LPDGSFEDWSVEELIV 153
           LPDGS+EDW V+ELI+
Sbjct: 110 LPDGSYEDWGVDELII 125


>2WB1-I nrPDB
          Length = 95

 Score = 53.5 bits (127), Expect = 3e-08,   Method: Compositional matrix adjust.
 Identities = 25/59 (42%), Positives = 38/59 (64%), Gaps = 2/59 (3%)

Query: 85  MTKYERARILGTRALQISMNAPVFVDLE--GETDPLRIAMKELAEKKIPLVIRRYLPDG 141
           +T+YE AR++  RALQ++M AP  +D+     TD + IA +E     +P+ IRR LP+G
Sbjct: 27  LTRYEIARVISARALQLAMGAPALIDINNLSSTDVISIAEEEFRRGVLPITIRRRLPNG 85


>3HI0-A nrPDB
          Length = 508

 Score = 28.9 bits (63), Expect = 1.1,   Method: Composition-based stats.
 Identities = 22/87 (25%), Positives = 41/87 (47%), Gaps = 17/87 (19%)

Query: 72  KAIPKDQRATTPYMTKYERARILGTRALQISMNA---------PVFVDLEGETDPLRIAM 122
           +A+ K++R+  P+     R  +   +  +I+ +A          +  + E E+DPL +A 
Sbjct: 263 QAVSKNRRSLLPFGAIAMREVLRAMKPAKIAFSAQGVREGYLYSLLTEAERESDPLLVAA 322

Query: 123 KELAEKKIPLVIRRYLPDGSFE--DWS 147
            ELA      ++R   P+ + E  DWS
Sbjct: 323 DELA------ILRARSPEHARELADWS 343


>4GKB-A nrPDB
          Length = 258

 Score = 28.5 bits (62), Expect = 1.1,   Method: Compositional matrix adjust.
 Identities = 11/24 (45%), Positives = 16/24 (66%)

Query: 122 MKELAEKKIPLVIRRYLPDGSFED 145
           M+   E+ IP+V  R+ PDG+F D
Sbjct: 25  MRLAEERAIPVVFARHAPDGAFLD 48


>6IGZ-B nrPDB
          Length = 734

 Score = 28.5 bits (62), Expect = 1.2,   Method: Composition-based stats.
 Identities = 13/40 (32%), Positives = 19/40 (47%)

Query: 33 PQFKDGETTDANGKTIVTGGNGPEDFQQHEQIRRKTLKEK 72
          P+F  G   D   + I  G     DF+ H+ I  +TL +K
Sbjct: 6  PKFSQGLAQDPTTRRIWFGVATAHDFESHDSITEETLYQK 45


>3SGZ-A nrPDB
          Length = 352

 Score = 27.7 bits (60), Expect = 2.1,   Method: Compositional matrix adjust.
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 8/63 (12%)

Query: 89  ERARILGTRALQISMNAPVFVD--------LEGETDPLRIAMKELAEKKIPLVIRRYLPD 140
           +RA  LG +AL I+++ PV  +        L  E + L+ A++ L E+K    +    P 
Sbjct: 142 QRAEALGFKALVITIDTPVLGNRRRDKRNQLNLEANILKAALRALKEEKPTQSVPVLFPK 201

Query: 141 GSF 143
            SF
Sbjct: 202 ASF 204


>3IHM-A nrPDB
          Length = 430

 Score = 27.7 bits (60), Expect = 2.4,   Method: Compositional matrix adjust.
 Identities = 11/21 (52%), Positives = 14/21 (66%)

Query: 33  PQFKDGETTDANGKTIVTGGN 53
           P F+DG  T  NGKTI+  G+
Sbjct: 295 PAFRDGHATLNNGKTIIGLGD 315


>2LAA-A nrPDB
          Length = 104

 Score = 26.6 bits (57), Expect = 5.1,   Method: Compositional matrix adjust.
 Identities = 10/24 (41%), Positives = 16/24 (66%)

Query: 1  MSDYEEAFNDGNENFEDFDVEHFS 24
           S  E AFNDGN N++  + +++S
Sbjct: 55 ASQLEAAFNDGNNNWDSNNTKNYS 78


>1K3I-A nrPDB
          Length = 656

 Score = 25.8 bits (55), Expect = 8.0,   Method: Composition-based stats.
 Identities = 11/26 (42%), Positives = 15/26 (57%)

Query: 28  TYEEKPQFKDGETTDANGKTIVTGGN 53
           T  +   F  G + D NG+ +VTGGN
Sbjct: 237 TVTKHDMFCPGISMDGNGQIVVTGGN 262


>5HHK-A nrPDB
          Length = 305

 Score = 25.8 bits (55), Expect = 8.2,   Method: Compositional matrix adjust.
 Identities = 15/51 (29%), Positives = 24/51 (47%), Gaps = 5/51 (9%)

Query: 6   EAFNDGNENFEDFDVEHFSDEETYEE-----KPQFKDGETTDANGKTIVTG 51
           +  NDGN+   D D+E F D   +++         KDG+      K +V+G
Sbjct: 140 DMMNDGNDWIVDIDLEKFFDTVNHDKLMTIIGRTIKDGDVISIVRKYLVSG 190


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

5U5Q-F nrPDB                                                          233   2e-62
6A5U-F nrPDB                                                          221   1e-58
5U0S-f nrPDB                                                          176   4e-45
5IP9-F nrPDB                                                          148   8e-37
6EXV-F nrPDB                                                          130   2e-31
2WB1-I nrPDB                                                           89   8e-19

Sequences not found previously or not previously below threshold:

3SGZ-A nrPDB                                                           28   1.9  
5ITV-A nrPDB                                                           27   2.7  
4GKB-A nrPDB                                                           26   5.4  


CONVERGED!
>5U5Q-F nrPDB
          Length = 155

 Score =  233 bits (595), Expect = 2e-62,   Method: Composition-based stats.
 Identities = 155/155 (100%), Positives = 155/155 (100%)

Query: 1   MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ 60
           MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ
Sbjct: 1   MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQ 60

Query: 61  HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI 120
           HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI
Sbjct: 61  HEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRI 120

Query: 121 AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL 155
           AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL
Sbjct: 121 AMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL 155


>6A5U-F nrPDB
          Length = 155

 Score =  221 bits (562), Expect = 1e-58,   Method: Composition-based stats.
 Identities = 91/151 (60%), Positives = 115/151 (76%), Gaps = 1/151 (0%)

Query: 5   EEAFNDGNENFEDFDVEHFSDEETYEEKPQFKD-GETTDANGKTIVTGGNGPEDFQQHEQ 63
           +EAFN+  ENFE+F+ EHFSD+   +   Q +D      A+G+ I+ G    E     + 
Sbjct: 4   DEAFNEQTENFENFEDEHFSDDNFEDRSTQPEDYAVGVTADGRQIINGDGIQEVNGTIKA 63

Query: 64  IRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMK 123
            R+++ KE AI K++R TTPY+TKYERARILGTRALQISMNAPV VD+EGETDPL+IAMK
Sbjct: 64  HRKRSNKELAILKEERTTTPYLTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMK 123

Query: 124 ELAEKKIPLVIRRYLPDGSFEDWSVEELIVD 154
           EL+++KIPLVIRRYLPDGS+EDW  +ELIVD
Sbjct: 124 ELSQRKIPLVIRRYLPDGSYEDWGCDELIVD 154


>5U0S-f nrPDB
          Length = 142

 Score =  176 bits (445), Expect = 4e-45,   Method: Composition-based stats.
 Identities = 89/155 (57%), Positives = 104/155 (67%), Gaps = 16/155 (10%)

Query: 1   MSDYEE--AFN-DGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPED 57
           MSDYEE  AF  DG    E+ D     DE    ++     GE +     T++T     ED
Sbjct: 1   MSDYEEDEAFGMDGAVMEEEVDELEMIDENGQSQQGVSHPGEPST----TVIT-----ED 51

Query: 58  FQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDP 117
               +  +      KA+ K+ R TTPYMTKYERARILGTRALQISMNAPV VDLEGETDP
Sbjct: 52  VASSKTAQSG----KAVAKEDRTTTPYMTKYERARILGTRALQISMNAPVLVDLEGETDP 107

Query: 118 LRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELI 152
           L+IAMKELA+KKIPL++RRYLPDGS+EDWSV ELI
Sbjct: 108 LQIAMKELAQKKIPLLVRRYLPDGSYEDWSVAELI 142


>5IP9-F nrPDB
          Length = 87

 Score =  148 bits (374), Expect = 8e-37,   Method: Composition-based stats.
 Identities = 87/87 (100%), Positives = 87/87 (100%)

Query: 69  LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK 128
           LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK
Sbjct: 1   LKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEK 60

Query: 129 KIPLVIRRYLPDGSFEDWSVEELIVDL 155
           KIPLVIRRYLPDGSFEDWSVEELIVDL
Sbjct: 61  KIPLVIRRYLPDGSFEDWSVEELIVDL 87


>6EXV-F nrPDB
          Length = 127

 Score =  130 bits (327), Expect = 2e-31,   Method: Composition-based stats.
 Identities = 60/83 (72%), Positives = 71/83 (85%)

Query: 71  EKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKI 130
           E+     +R TTPYMTKYERAR+LGTRALQI+M APV V+LEGETDPL IAMKEL  +KI
Sbjct: 43  ERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKI 102

Query: 131 PLVIRRYLPDGSFEDWSVEELIV 153
           P++IRRYLPDGS+EDW V+ELI+
Sbjct: 103 PIIIRRYLPDGSYEDWGVDELII 125


>2WB1-I nrPDB
          Length = 95

 Score = 89.0 bits (219), Expect = 8e-19,   Method: Composition-based stats.
 Identities = 25/59 (42%), Positives = 38/59 (64%), Gaps = 2/59 (3%)

Query: 85  MTKYERARILGTRALQISMNAPVFVDLE--GETDPLRIAMKELAEKKIPLVIRRYLPDG 141
           +T+YE AR++  RALQ++M AP  +D+     TD + IA +E     +P+ IRR LP+G
Sbjct: 27  LTRYEIARVISARALQLAMGAPALIDINNLSSTDVISIAEEEFRRGVLPITIRRRLPNG 85


>3SGZ-A nrPDB
          Length = 352

 Score = 27.8 bits (60), Expect = 1.9,   Method: Composition-based stats.
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 8/63 (12%)

Query: 89  ERARILGTRALQISMNAPVFVD--------LEGETDPLRIAMKELAEKKIPLVIRRYLPD 140
           +RA  LG +AL I+++ PV  +        L  E + L+ A++ L E+K    +    P 
Sbjct: 142 QRAEALGFKALVITIDTPVLGNRRRDKRNQLNLEANILKAALRALKEEKPTQSVPVLFPK 201

Query: 141 GSF 143
            SF
Sbjct: 202 ASF 204


>5ITV-A nrPDB
          Length = 255

 Score = 27.4 bits (59), Expect = 2.7,   Method: Composition-based stats.
 Identities = 12/67 (17%), Positives = 24/67 (35%), Gaps = 2/67 (2%)

Query: 1   MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETT--DANGKTIVTGGNGPEDF 58
           ++D +EA  +     E+ D  HF   +  +E       E+      G  ++    G E  
Sbjct: 36  VADIDEAQGEAMVRKENNDRLHFVQTDITDEAACQHAVESAVHTFGGLDVLINNAGIEIV 95

Query: 59  QQHEQIR 65
               ++ 
Sbjct: 96  APIHEME 102


>4GKB-A nrPDB
          Length = 258

 Score = 26.2 bits (56), Expect = 5.4,   Method: Composition-based stats.
 Identities = 11/24 (45%), Positives = 16/24 (66%)

Query: 122 MKELAEKKIPLVIRRYLPDGSFED 145
           M+   E+ IP+V  R+ PDG+F D
Sbjct: 25  MRLAEERAIPVVFARHAPDGAFLD 48


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.310    0.128    0.329 

Lambda     K      H
   0.267   0.0371    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,865,852
Number of Sequences: 36641
Number of extensions: 222043
Number of successful extensions: 527
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 497
Number of HSP's gapped (non-prelim): 40
length of query: 155
length of database: 10,336,785
effective HSP length: 89
effective length of query: 66
effective length of database: 7,075,736
effective search space: 466998576
effective search space used: 466998576
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 54 (25.6 bits)