BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P20292 (161 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2Q7R-A nrPDB 327 1e-90 4NTA-A nrPDB 75 1e-14 4J7T-A nrPDB 74 3e-14 4YOJ-A nrPDB 26 7.6 >2Q7R-A nrPDB Length = 161 Score = 327 bits (839), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 160/161 (99%), Positives = 160/161 (99%) Query: 1 MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNC 60 MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNC Sbjct: 1 MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNC 60 Query: 61 VDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIIL 120 VDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIIL Sbjct: 61 VDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIIL 120 Query: 121 FLFLMSVAGIFNYYLIFFFGSDFENYIKTISTTISPLLLIP 161 FLFLMSVAGIFNYYLIFFFGSDFENYI TISTTISPLLLIP Sbjct: 121 FLFLMSVAGIFNYYLIFFFGSDFENYIATISTTISPLLLIP 161 >4NTA-A nrPDB Length = 156 Score = 75.1 bits (183), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 47/129 (36%), Positives = 67/129 (51%), Gaps = 2/129 (1%) Query: 8 NVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNCVDAYPTF 67 V LLA VTL+ V+ +F+ +V R + +G FERV+ A NC + +P F Sbjct: 10 EVALLATVTLVGVLLQAYFSLQVISARRAFHVSPPLTSGPPEFERVFRAQVNCSEYFPLF 69 Query: 68 LAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYL-GERTQSTPGYIFGKRIILFLFLMS 126 LA LW AG+ + AA GL YLF R +YF GY + + TP Y R + L M+ Sbjct: 70 LATLWVAGIFFHEGAAALCGLFYLFARLRYFQGYARSAQLRLTPLYA-SARALWLLVAMA 128 Query: 127 VAGIFNYYL 135 G+ ++L Sbjct: 129 ALGLLVHFL 137 >4J7T-A nrPDB Length = 156 Score = 73.9 bits (180), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 41/94 (43%), Positives = 52/94 (55%) Query: 8 NVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNCVDAYPTF 67 V LLA VTL+ V+ +F+ +V R TG FERVY A NC + +P F Sbjct: 10 EVALLAAVTLLGVLLQAYFSLQVISARRAFRVSPPLTTGPPEFERVYRAQVNCSEYFPLF 69 Query: 68 LAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGY 101 LA LW AG+ + AA GL+YLF R +YF GY Sbjct: 70 LATLWVAGIFFHEGAAALCGLVYLFARLRYFQGY 103 >4YOJ-A nrPDB Length = 306 Score = 26.2 bits (56), Expect = 7.6, Method: Compositional matrix adjust. Identities = 17/52 (32%), Positives = 21/52 (40%) Query: 21 VQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNCVDAYPTFLAVLW 72 V N + H++E + T G SF AFE T D Y T V W Sbjct: 159 VINFVYMHQMELSNGTHTGSSFDGVMYGAFEDKQTHQLQLTDKYCTINVVAW 210 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2Q7R-A nrPDB 242 4e-65 4NTA-A nrPDB 163 4e-41 4J7T-A nrPDB 144 1e-35 Sequences not found previously or not previously below threshold: 3PLX-B nrPDB 28 1.6 5K5S-A nrPDB 28 2.5 5FBH-B nrPDB 27 4.1 CONVERGED! >2Q7R-A nrPDB Length = 161 Score = 242 bits (619), Expect = 4e-65, Method: Composition-based stats. Identities = 160/161 (99%), Positives = 160/161 (99%) Query: 1 MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNC 60 MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNC Sbjct: 1 MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNC 60 Query: 61 VDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIIL 120 VDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIIL Sbjct: 61 VDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIIL 120 Query: 121 FLFLMSVAGIFNYYLIFFFGSDFENYIKTISTTISPLLLIP 161 FLFLMSVAGIFNYYLIFFFGSDFENYI TISTTISPLLLIP Sbjct: 121 FLFLMSVAGIFNYYLIFFFGSDFENYIATISTTISPLLLIP 161 >4NTA-A nrPDB Length = 156 Score = 163 bits (412), Expect = 4e-41, Method: Composition-based stats. Identities = 47/129 (36%), Positives = 67/129 (51%), Gaps = 2/129 (1%) Query: 8 NVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNCVDAYPTF 67 V LLA VTL+ V+ +F+ +V R + +G FERV+ A NC + +P F Sbjct: 10 EVALLATVTLVGVLLQAYFSLQVISARRAFHVSPPLTSGPPEFERVFRAQVNCSEYFPLF 69 Query: 68 LAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYL-GERTQSTPGYIFGKRIILFLFLMS 126 LA LW AG+ + AA GL YLF R +YF GY + + TP Y R + L M+ Sbjct: 70 LATLWVAGIFFHEGAAALCGLFYLFARLRYFQGYARSAQLRLTPLYA-SARALWLLVAMA 128 Query: 127 VAGIFNYYL 135 G+ ++L Sbjct: 129 ALGLLVHFL 137 >4J7T-A nrPDB Length = 156 Score = 144 bits (364), Expect = 1e-35, Method: Composition-based stats. Identities = 43/107 (40%), Positives = 56/107 (52%), Gaps = 1/107 (0%) Query: 8 NVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNCVDAYPTF 67 V LLA VTL+ V+ +F+ +V R TG FERVY A NC + +P F Sbjct: 10 EVALLAAVTLLGVLLQAYFSLQVISARRAFRVSPPLTTGPPEFERVYRAQVNCSEYFPLF 69 Query: 68 LAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYL-GERTQSTPGYI 113 LA LW AG+ + AA GL+YLF R +YF GY + + P Y Sbjct: 70 LATLWVAGIFFHEGAAALCGLVYLFARLRYFQGYARSAQLRLAPLYA 116 >3PLX-B nrPDB Length = 102 Score = 28.4 bits (62), Expect = 1.6, Method: Composition-based stats. Identities = 12/42 (28%), Positives = 19/42 (45%) Query: 43 QRTGTLAFERVYTANQNCVDAYPTFLAVLWSAGLLCSQVPAA 84 Q +G L +E+V N N + T+ G++C AA Sbjct: 10 QASGILEYEKVQVVNVNNGARFETYTIATQEEGVVCLNGAAA 51 >5K5S-A nrPDB Length = 615 Score = 27.6 bits (60), Expect = 2.5, Method: Composition-based stats. Identities = 11/33 (33%), Positives = 17/33 (51%) Query: 59 NCVDAYPTFLAVLWSAGLLCSQVPAAFAGLMYL 91 NC + P+ +AV+ + G S A GL Y+ Sbjct: 130 NCSEHIPSTIAVVGATGSGVSTAVANLLGLFYI 162 >5FBH-B nrPDB Length = 568 Score = 26.9 bits (58), Expect = 4.1, Method: Composition-based stats. Identities = 11/33 (33%), Positives = 17/33 (51%) Query: 59 NCVDAYPTFLAVLWSAGLLCSQVPAAFAGLMYL 91 NC + P+ +AV+ + G S A GL Y+ Sbjct: 157 NCSEHIPSTIAVVGATGSGVSTAVANLLGLFYI 189 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.323 0.141 0.412 Lambda K H 0.267 0.0437 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,505,903 Number of Sequences: 36641 Number of extensions: 253834 Number of successful extensions: 635 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 618 Number of HSP's gapped (non-prelim): 21 length of query: 161 length of database: 10,336,785 effective HSP length: 89 effective length of query: 72 effective length of database: 7,075,736 effective search space: 509452992 effective search space used: 509452992 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.4 bits) S2: 55 (25.7 bits)