BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P20052 (293 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2PK9-B nrPDB 602 e-173 5H8N-A nrPDB 30 1.0 6G19-A nrPDB 30 1.1 5OOL-Y nrPDB 30 1.1 4KRD-B nrPDB 29 2.3 3B55-A nrPDB 28 3.2 1I9Z-A nrPDB 27 7.6 >2PK9-B nrPDB Length = 293 Score = 602 bits (1552), Expect = e-173, Method: Compositional matrix adjust. Identities = 293/293 (100%), Positives = 293/293 (100%) Query: 1 MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLVSLIAINENSATKKSDD 60 MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLVSLIAINENSATKKSDD Sbjct: 1 MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLVSLIAINENSATKKSDD 60 Query: 61 QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH 120 QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH Sbjct: 61 QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH 120 Query: 121 RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC 180 RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC Sbjct: 121 RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC 180 Query: 181 SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRK 240 SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRK Sbjct: 181 SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRK 240 Query: 241 VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD 293 VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD Sbjct: 241 VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD 293 >5H8N-A nrPDB Length = 285 Score = 30.4 bits (67), Expect = 1.0, Method: Compositional matrix adjust. Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%) Query: 221 YRRIVQLVGSFNASPDKSRKVDYVLP---PNIDIVSESGSQTTQL--KGSSSPNSHSSQK 275 Y+R V VGS + ++S VD+ +P I ++ G+Q T L K P+ +S Sbjct: 104 YQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRGTQVTGLSDKKFQRPHDYSPPF 163 Query: 276 RYS 278 R+ Sbjct: 164 RFG 166 >6G19-A nrPDB Length = 696 Score = 30.4 bits (67), Expect = 1.1, Method: Compositional matrix adjust. Identities = 26/100 (26%), Positives = 42/100 (42%), Gaps = 16/100 (16%) Query: 102 IDLLQTVYPDFTLNSLTAHRFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILEND 161 I L T+ FT + ++ + T T +T L NA +A+V GV+ H L Sbjct: 378 IKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEV-GVKAHHL------ 430 Query: 162 FLKRVNYRIIPRDHNITLCSIEQKQKKFVIDKNALGSLDL 201 I H+ + + Q ++K VI K G ++L Sbjct: 431 ---------IGAGHSSEVKPMTQTEQKEVISKFRTGEINL 461 >5OOL-Y nrPDB Length = 250 Score = 30.0 bits (66), Expect = 1.1, Method: Compositional matrix adjust. Identities = 25/94 (26%), Positives = 49/94 (52%), Gaps = 11/94 (11%) Query: 100 YYIDLLQTVYPDFTLNSLTAHRFLLTATTVATKGLCDSFSTNAHYA----KVGGVR-CHE 154 +++ LL P T N + H++ L TT++ KGL + F ++ K G C + Sbjct: 34 FFLSLL----PKSTPNVTSFHQYRLLHTTLSRKGLEEFFDDPKNWGQEKVKSGAAWTCQQ 89 Query: 155 LNILENDFLKRVNYRIIPRDHNITLCSIEQKQKK 188 L N+ L ++ Y ++ ++ N+ L ++EQ+ K+ Sbjct: 90 LRNKSNEDLHKLWY-VLLKERNM-LLTLEQEAKR 121 >4KRD-B nrPDB Length = 207 Score = 29.3 bits (64), Expect = 2.3, Method: Compositional matrix adjust. Identities = 23/104 (22%), Positives = 44/104 (42%), Gaps = 7/104 (6%) Query: 67 YHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLL-------QTVYPDFTLNSLTA 119 ++ K P +S ++ R+ + V + + + ID L + L Sbjct: 74 FYMKSKPTLSSADFLKRIQDKCEYQPTVYLVATFLIDTLFLTRDGNNILQLKLNLQEKEV 133 Query: 120 HRFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFL 163 HR ++ A ++TK L D ++ +++KV G+ L LE L Sbjct: 134 HRMIIAAVRLSTKLLEDFVHSHEYFSKVCGISKRLLTKLEVSLL 177 >3B55-A nrPDB Length = 451 Score = 28.5 bits (62), Expect = 3.2, Method: Compositional matrix adjust. Identities = 22/95 (23%), Positives = 43/95 (45%), Gaps = 15/95 (15%) Query: 156 NILENDFLKRVNYRIIPR-------------DHNITLCSIEQKQKKFVIDKNALGSLDLD 202 NI+E ++K N +++PR D N +++++K+V+D + +L + Sbjct: 183 NIIE--YIKANNSKLLPRVEEKIKGLIPVTKDMNTFESLTKEEKEKYVLDAKTISALLEE 240 Query: 203 SYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDK 237 + SY+N + + + R I Q PDK Sbjct: 241 NKSYLNGKSKEFAWIKQNARIIEQFTTMLATPPDK 275 >1I9Z-A nrPDB Length = 347 Score = 27.3 bits (59), Expect = 7.6, Method: Compositional matrix adjust. Identities = 13/42 (30%), Positives = 22/42 (52%) Query: 153 HELNILENDFLKRVNYRIIPRDHNITLCSIEQKQKKFVIDKN 194 HEL EN+F + N +I +N+ CS K + ++ +N Sbjct: 10 HELRKRENEFSEHKNVKIFVASYNLNGCSATTKLENWLFPEN 51 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2PK9-B nrPDB 612 e-176 Sequences not found previously or not previously below threshold: 3B55-A nrPDB 31 0.56 5H8N-A nrPDB 31 0.59 6G19-A nrPDB 30 1.2 5OOL-Y nrPDB 30 1.7 1I9Z-A nrPDB 28 4.8 4EUF-A nrPDB 27 9.3 CONVERGED! >2PK9-B nrPDB Length = 293 Score = 612 bits (1577), Expect = e-176, Method: Composition-based stats. Identities = 293/293 (100%), Positives = 293/293 (100%) Query: 1 MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLVSLIAINENSATKKSDD 60 MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLVSLIAINENSATKKSDD Sbjct: 1 MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLVSLIAINENSATKKSDD 60 Query: 61 QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH 120 QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH Sbjct: 61 QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH 120 Query: 121 RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC 180 RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC Sbjct: 121 RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC 180 Query: 181 SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRK 240 SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRK Sbjct: 181 SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRK 240 Query: 241 VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD 293 VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD Sbjct: 241 VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD 293 >3B55-A nrPDB Length = 451 Score = 31.2 bits (69), Expect = 0.56, Method: Composition-based stats. Identities = 25/107 (23%), Positives = 48/107 (44%), Gaps = 17/107 (15%) Query: 156 NILENDFLKRVNYRIIPR-------------DHNITLCSIEQKQKKFVIDKNALGSLDLD 202 NI+E ++K N +++PR D N +++++K+V+D + +L + Sbjct: 183 NIIE--YIKANNSKLLPRVEEKIKGLIPVTKDMNTFESLTKEEKEKYVLDAKTISALLEE 240 Query: 203 SYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRKVDYVLPPNI 249 + SY+N + + + R I Q PDK D+ L +I Sbjct: 241 NKSYLNGKSKEFAWIKQNARIIEQFTTMLATPPDKP--ADFYLKHDI 285 >5H8N-A nrPDB Length = 285 Score = 31.2 bits (69), Expect = 0.59, Method: Composition-based stats. Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 5/62 (8%) Query: 221 YRRIVQLVGSFNASPDKSRKVDYVLP---PNIDIVSESGSQTTQL--KGSSSPNSHSSQK 275 Y+R V VGS + ++S VD+ +P I ++ G+Q T L K P+ +S Sbjct: 104 YQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRGTQVTGLSDKKFQRPHDYSPPF 163 Query: 276 RY 277 R+ Sbjct: 164 RF 165 >6G19-A nrPDB Length = 696 Score = 30.0 bits (66), Expect = 1.2, Method: Composition-based stats. Identities = 26/100 (26%), Positives = 42/100 (42%), Gaps = 16/100 (16%) Query: 102 IDLLQTVYPDFTLNSLTAHRFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILEND 161 I L T+ FT + ++ + T T +T L NA +A+V GV+ H L Sbjct: 378 IKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEV-GVKAHHL------ 430 Query: 162 FLKRVNYRIIPRDHNITLCSIEQKQKKFVIDKNALGSLDL 201 I H+ + + Q ++K VI K G ++L Sbjct: 431 ---------IGAGHSSEVKPMTQTEQKEVISKFRTGEINL 461 >5OOL-Y nrPDB Length = 250 Score = 29.7 bits (65), Expect = 1.7, Method: Composition-based stats. Identities = 25/94 (26%), Positives = 49/94 (52%), Gaps = 11/94 (11%) Query: 100 YYIDLLQTVYPDFTLNSLTAHRFLLTATTVATKGLCDSFSTNAHYA----KVGGV-RCHE 154 +++ LL P T N + H++ L TT++ KGL + F ++ K G C + Sbjct: 34 FFLSLL----PKSTPNVTSFHQYRLLHTTLSRKGLEEFFDDPKNWGQEKVKSGAAWTCQQ 89 Query: 155 LNILENDFLKRVNYRIIPRDHNITLCSIEQKQKK 188 L N+ L ++ Y ++ ++ N+ L ++EQ+ K+ Sbjct: 90 LRNKSNEDLHKLWY-VLLKERNMLL-TLEQEAKR 121 >1I9Z-A nrPDB Length = 347 Score = 28.1 bits (61), Expect = 4.8, Method: Composition-based stats. Identities = 13/42 (30%), Positives = 22/42 (52%) Query: 153 HELNILENDFLKRVNYRIIPRDHNITLCSIEQKQKKFVIDKN 194 HEL EN+F + N +I +N+ CS K + ++ +N Sbjct: 10 HELRKRENEFSEHKNVKIFVASYNLNGCSATTKLENWLFPEN 51 >4EUF-A nrPDB Length = 418 Score = 27.0 bits (58), Expect = 9.3, Method: Composition-based stats. Identities = 24/95 (25%), Positives = 40/95 (42%), Gaps = 3/95 (3%) Query: 137 SFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLCSIEQKQKKFVIDKNAL 196 S+ T A ++G + NI +F K+ ++ ++ S E K K K+ Sbjct: 93 SYETGATDRRIGTAGWYN-NIFFKEFAKKKG--LVAKNFIEDAFSNETKDKVIKYIKDEF 149 Query: 197 GSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSF 231 G +DL YS + Y + Y RI ++G F Sbjct: 150 GKIDLFVYSLAAPRRKDYKTGNVYTSRIKTILGDF 184 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.132 0.376 Lambda K H 0.267 0.0409 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,946,275 Number of Sequences: 36641 Number of extensions: 421446 Number of successful extensions: 913 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 904 Number of HSP's gapped (non-prelim): 17 length of query: 293 length of database: 10,336,785 effective HSP length: 96 effective length of query: 197 effective length of database: 6,819,249 effective search space: 1343392053 effective search space used: 1343392053 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 59 (27.3 bits)