BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P19518 (222 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3JBR-E nrPDB 462 e-131 5KK2-E nrPDB 44 4e-05 6QKZ-I nrPDB 35 0.017 2FYO-A nrPDB 29 1.6 >3JBR-E nrPDB Length = 222 Score = 462 bits (1188), Expect = e-131, Method: Compositional matrix adjust. Identities = 222/222 (100%), Positives = 222/222 (100%) Query: 1 MSPTEAPKVRVTLFCILVGIVLAMTAVVSDHWAVLSPHMENHNTTCEAAHFGLWRICTKR 60 MSPTEAPKVRVTLFCILVGIVLAMTAVVSDHWAVLSPHMENHNTTCEAAHFGLWRICTKR Sbjct: 1 MSPTEAPKVRVTLFCILVGIVLAMTAVVSDHWAVLSPHMENHNTTCEAAHFGLWRICTKR 60 Query: 61 IALGEDRSCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVFSLGFLIM 120 IALGEDRSCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVFSLGFLIM Sbjct: 61 IALGEDRSCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVFSLGFLIM 120 Query: 121 GTICALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSW 180 GTICALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSW Sbjct: 121 GTICALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSW 180 Query: 181 SFACACAAFVLLFLGGISLLLFSLPRMPQNPWESCMDAEPEH 222 SFACACAAFVLLFLGGISLLLFSLPRMPQNPWESCMDAEPEH Sbjct: 181 SFACACAAFVLLFLGGISLLLFSLPRMPQNPWESCMDAEPEH 222 >5KK2-E nrPDB Length = 323 Score = 44.3 bits (103), Expect = 4e-05, Method: Compositional matrix adjust. Identities = 51/201 (25%), Positives = 79/201 (39%), Gaps = 29/201 (14%) Query: 22 LAMTAVVSDHW----AVLSPHMENHNTTCEA-----AHFGLWRICTKRIALGEDRSCGPI 72 L AV +D+W V + N T + H GLWR C C Sbjct: 23 LMTIAVGTDYWLYSRGVCKTKSVSENETSKKNEEVMTHSGLWRTC-----------CLEG 71 Query: 73 TLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVF---SLGFLIMGTIC-ALMA 128 G C HF P ++ +E T + + + A S+F S+ L MG +C A Sbjct: 72 NFKGL--CKQIDHF-PEDAD--YEADTAEYFLRAVRASSIFPILSVILLFMGGLCIAASE 126 Query: 129 FRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSWSFACACAA 188 F K R ++ A +F+V AGL + + V + D+ Y Y WSF + Sbjct: 127 FYKTRHNIILSAGIFFVSAGLSNIIGIIVYISANAGDPSKSDSKKNSYSYGWSFYFGALS 186 Query: 189 FVLLFLGGISLLLFSLPRMPQ 209 F++ + G+ + + R Q Sbjct: 187 FIIAEMVGVLAVHMFIDRHKQ 207 >6QKZ-I nrPDB Length = 423 Score = 35.4 bits (80), Expect = 0.017, Method: Compositional matrix adjust. Identities = 41/159 (25%), Positives = 68/159 (42%), Gaps = 32/159 (20%) Query: 50 HFGLWRICTKRIALGEDRSCGPITLPGEKN--CSYFRHFNPGESSEIFEFTTQKEYSISA 107 H GLWRIC C L G K C HF P ++ + + + A Sbjct: 83 HSGLWRIC-----------C----LEGLKRGVCVKINHF-PEDTDYDHDSSEYLLRVVRA 126 Query: 108 AAI-SVFSLGFLIMGTIC--ALMAFRKKRDYLLRPASMFYVFAGLC------LFVSLEVM 158 ++I + S L++G +C A ++ KR+ +L A + +V AGL +++S Sbjct: 127 SSIFPILSAILLLLGGVCVAASRVYKSKRNIIL-GAGILFVAAGLSNIIGVIVYISANAG 185 Query: 159 RQSVKRMIDSEDTVWIEYYYSWSFACACAAFVLLFLGGI 197 KR + ++ Y Y WSF +F+L + G+ Sbjct: 186 EPGPKRDEEKKN----HYSYGWSFYFGGLSFILAEVIGV 220 >2FYO-A nrPDB Length = 653 Score = 28.9 bits (63), Expect = 1.6, Method: Compositional matrix adjust. Identities = 19/55 (34%), Positives = 27/55 (49%), Gaps = 7/55 (12%) Query: 96 EFTTQKEYSISAAAISVFSLGFLIM--GTI-----CALMAFRKKRDYLLRPASMF 143 EF +K+ S A A F + FL T+ C+ AF+ R +RPAS+F Sbjct: 449 EFLKKKQLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASIF 503 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3JBR-E nrPDB 369 e-103 5KK2-E nrPDB 236 6e-63 Sequences not found previously or not previously below threshold: 6QKZ-I nrPDB 145 1e-35 2MOZ-A nrPDB 32 0.25 1T8Q-A nrPDB 30 1.0 5WT2-A nrPDB 28 3.8 1LDM-A nrPDB 27 5.7 >3JBR-E nrPDB Length = 222 Score = 369 bits (947), Expect = e-103, Method: Composition-based stats. Identities = 222/222 (100%), Positives = 222/222 (100%) Query: 1 MSPTEAPKVRVTLFCILVGIVLAMTAVVSDHWAVLSPHMENHNTTCEAAHFGLWRICTKR 60 MSPTEAPKVRVTLFCILVGIVLAMTAVVSDHWAVLSPHMENHNTTCEAAHFGLWRICTKR Sbjct: 1 MSPTEAPKVRVTLFCILVGIVLAMTAVVSDHWAVLSPHMENHNTTCEAAHFGLWRICTKR 60 Query: 61 IALGEDRSCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVFSLGFLIM 120 IALGEDRSCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVFSLGFLIM Sbjct: 61 IALGEDRSCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVFSLGFLIM 120 Query: 121 GTICALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSW 180 GTICALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSW Sbjct: 121 GTICALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSW 180 Query: 181 SFACACAAFVLLFLGGISLLLFSLPRMPQNPWESCMDAEPEH 222 SFACACAAFVLLFLGGISLLLFSLPRMPQNPWESCMDAEPEH Sbjct: 181 SFACACAAFVLLFLGGISLLLFSLPRMPQNPWESCMDAEPEH 222 >5KK2-E nrPDB Length = 323 Score = 236 bits (602), Expect = 6e-63, Method: Composition-based stats. Identities = 51/201 (25%), Positives = 79/201 (39%), Gaps = 29/201 (14%) Query: 22 LAMTAVVSDHW----AVLSPHMENHNTTCEA-----AHFGLWRICTKRIALGEDRSCGPI 72 L AV +D+W V + N T + H GLWR C C Sbjct: 23 LMTIAVGTDYWLYSRGVCKTKSVSENETSKKNEEVMTHSGLWRTC-----------CLEG 71 Query: 73 TLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVF---SLGFLIMGTIC-ALMA 128 G C HF P ++ +E T + + + A S+F S+ L MG +C A Sbjct: 72 NFKGL--CKQIDHF-PEDAD--YEADTAEYFLRAVRASSIFPILSVILLFMGGLCIAASE 126 Query: 129 FRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSWSFACACAA 188 F K R ++ A +F+V AGL + + V + D+ Y Y WSF + Sbjct: 127 FYKTRHNIILSAGIFFVSAGLSNIIGIIVYISANAGDPSKSDSKKNSYSYGWSFYFGALS 186 Query: 189 FVLLFLGGISLLLFSLPRMPQ 209 F++ + G+ + + R Q Sbjct: 187 FIIAEMVGVLAVHMFIDRHKQ 207 >6QKZ-I nrPDB Length = 423 Score = 145 bits (366), Expect = 1e-35, Method: Composition-based stats. Identities = 47/237 (19%), Positives = 85/237 (35%), Gaps = 43/237 (18%) Query: 8 KVRVTLFCILVGIVLAMTAVVSDHWA-----VLS-----------PHMENHNTTCEA--- 48 +V +T L A+ +D+W + + PH ++ + Sbjct: 20 QVLLTTIGAFAAFGLMTIAISTDYWLYTRALICNTTNLTAGDDGPPHRGGSGSSEKKDPG 79 Query: 49 --AHFGLWRICTKRIALGEDRSCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSIS 106 H GLWRIC C G C HF P ++ ++ + + Sbjct: 80 GLTHSGLWRIC-----------CLEGLKRGV--CVKINHF-PEDTD--YDHDSSEYLLRV 123 Query: 107 AAAISVF---SLGFLIMGTIC-ALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSV 162 A S+F S L++G +C A K + ++ A + +V AGL + + V + Sbjct: 124 VRASSIFPILSAILLLLGGVCVAASRVYKSKRNIILGAGILFVAAGLSNIIGVIVYISAN 183 Query: 163 KRM--IDSEDTVWIEYYYSWSFACACAAFVLLFLGGISLLLFSLPRMPQNPWESCMD 217 ++ Y Y WSF +F+L + G+ + + R + +S D Sbjct: 184 AGEPGPKRDEEKKNHYSYGWSFYFGGLSFILAEVIGVLAVNIYIERSREAHCQSRSD 240 >2MOZ-A nrPDB Length = 81 Score = 31.6 bits (70), Expect = 0.25, Method: Composition-based stats. Identities = 18/65 (27%), Positives = 28/65 (43%), Gaps = 8/65 (12%) Query: 109 AISVFSLGFLIMGTICALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDS 168 A+S F+ +I+ + L A DY+L PA L +F+ L + KR D+ Sbjct: 19 ALSSFTPVLVILLGVVGLSALTGYLDYVLLPA--------LAIFIGLTIYAIQRKRQADA 70 Query: 169 EDTVW 173 T Sbjct: 71 SSTPK 75 >1T8Q-A nrPDB Length = 336 Score = 29.7 bits (65), Expect = 1.0, Method: Composition-based stats. Identities = 12/60 (20%), Positives = 16/60 (26%), Gaps = 1/60 (1%) Query: 151 LFVSLEVMRQSVKRMIDSEDTVWIEYYYSWSFACACAAFVLLFLGGISL-LLFSLPRMPQ 209 V L + D W+ Y Y W F V + GI + Q Sbjct: 209 NLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADGIGPDYHMLIEETSQ 268 >5WT2-A nrPDB Length = 401 Score = 27.8 bits (60), Expect = 3.8, Method: Composition-based stats. Identities = 26/115 (22%), Positives = 41/115 (35%), Gaps = 21/115 (18%) Query: 20 IVLAMTAVVSDHWAV-------LSPHMENHNTTCEAAHFGLWRICTKRIALGEDRSCGPI 72 +++ A S++W + +NH T A H + C +LG + + PI Sbjct: 67 VIITSCATESNNWVLKGVYFDECLKKGKNHIVTTVAEHPAVRSTCNFLESLGVEVTYLPI 126 Query: 73 TLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAA-AISVFSLGFLI--MGTIC 124 G R E T+K +S A + L F I +G IC Sbjct: 127 NEHGSITAEQVR-----------EAITEKTALVSVMWANNETGLIFPIEEIGAIC 170 >1LDM-A nrPDB Length = 329 Score = 27.4 bits (59), Expect = 5.7, Method: Composition-based stats. Identities = 17/89 (19%), Positives = 32/89 (35%), Gaps = 7/89 (7%) Query: 92 SEIFEFTTQKEYSISAAAISVFSLGFLIMGTIC-------ALMAFRKKRDYLLRPASMFY 144 +E K Y+ A +SV L IM +C + F +D + Sbjct: 233 DSAYEVIKLKGYTSWAIGLSVADLAETIMKNLCRVHPVSTMVKDFYGIKDNVFLSLPCVL 292 Query: 145 VFAGLCLFVSLEVMRQSVKRMIDSEDTVW 173 G+ V +++ +++ S T+W Sbjct: 293 NDHGISNIVKMKLKPNEEQQLQKSATTLW 321 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.323 0.129 0.387 Lambda K H 0.267 0.0395 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,261,635 Number of Sequences: 36641 Number of extensions: 283918 Number of successful extensions: 884 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 863 Number of HSP's gapped (non-prelim): 17 length of query: 222 length of database: 10,336,785 effective HSP length: 93 effective length of query: 129 effective length of database: 6,929,172 effective search space: 893863188 effective search space used: 893863188 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 57 (26.6 bits)