BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P17813 (658 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5HZW-A nrPDB 649 0.0 5HZV-A nrPDB 509 e-144 5VMT-A nrPDB 29 6.6 5EIY-B nrPDB 28 10.0 >5HZW-A nrPDB Length = 691 Score = 649 bits (1675), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/312 (100%), Positives = 312/312 (100%) Query: 26 ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQAS 85 ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQAS Sbjct: 380 ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQAS 439 Query: 86 KQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQ 145 KQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQ Sbjct: 440 KQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQ 499 Query: 146 ILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVR 205 ILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVR Sbjct: 500 ILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVR 559 Query: 206 GCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDA 265 GCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDA Sbjct: 560 GCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDA 619 Query: 266 NHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSL 325 NHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSL Sbjct: 620 NHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSL 679 Query: 326 HASSCGGRLQTS 337 HASSCGGRLQTS Sbjct: 680 HASSCGGRLQTS 691 >5HZV-A nrPDB Length = 626 Score = 509 bits (1312), Expect = e-144, Method: Compositional matrix adjust. Identities = 244/245 (99%), Positives = 245/245 (100%) Query: 337 SPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEA 396 +PAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEA Sbjct: 374 APAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEA 433 Query: 397 EDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYL 456 EDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYL Sbjct: 434 EDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYL 493 Query: 457 SPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNC 516 SPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNC Sbjct: 494 SPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNC 553 Query: 517 VSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIIS 576 VSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIIS Sbjct: 554 VSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIIS 613 Query: 577 PDLSG 581 PDLSG Sbjct: 614 PDLSG 618 >5VMT-A nrPDB Length = 342 Score = 28.9 bits (63), Expect = 6.6, Method: Compositional matrix adjust. Identities = 22/81 (27%), Positives = 35/81 (43%) Query: 563 EVHRTVFMRLNIISPDLSGCTSKGLVLPAVLGITFGAFLIGALLTAALWYIYSHTRSPSK 622 ++ R LNI+ + GLV+P + G G+ + T +L + S P+ Sbjct: 203 DLRRARAAALNIVPNSTGAAKAIGLVIPELNGKLDGSAQRVPVATGSLTELVSVLERPAT 262 Query: 623 REPVVAVAAPASSESSSTNHS 643 +E + A ASSES N Sbjct: 263 KEEINAAMKAASSESYGYNED 283 >5EIY-B nrPDB Length = 729 Score = 28.5 bits (62), Expect = 10.0, Method: Compositional matrix adjust. Identities = 30/129 (23%), Positives = 48/129 (37%), Gaps = 23/129 (17%) Query: 263 IDANHNMQIWTTGEYSFKIFPEKNIR--GFKLPDTPQGLLGEARMLNASIVASFVELPLA 320 I+A H +I+ + F ++ E ++ G LP A+ P + Sbjct: 157 IEAQHRHRIYCGADAEFDLWTEVDLSQSGVALP-----------------AAAIGTEPTS 199 Query: 321 SIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHL 380 I +L A + GR P I+T P D + L + D+A+ L L K A Sbjct: 200 FIAALTAQAESGR----PVEIRTPTPPDEATLRTLAQALGRPLPDEALPLALSKPWSAET 255 Query: 381 KCTITGLTF 389 T +T Sbjct: 256 GPTYARITL 264 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5HZW-A nrPDB 677 0.0 5HZV-A nrPDB 530 e-151 Sequences not found previously or not previously below threshold: 4S38-A nrPDB 33 0.27 5VMT-A nrPDB 31 1.6 2WOP-A nrPDB 30 4.9 3CLL-A nrPDB 29 6.6 5EIY-B nrPDB 29 6.9 3DUZ-A nrPDB 29 7.8 CONVERGED! >5HZW-A nrPDB Length = 691 Score = 677 bits (1746), Expect = 0.0, Method: Composition-based stats. Identities = 312/312 (100%), Positives = 312/312 (100%) Query: 26 ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQAS 85 ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQAS Sbjct: 380 ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQAS 439 Query: 86 KQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQ 145 KQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQ Sbjct: 440 KQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQ 499 Query: 146 ILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVR 205 ILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVR Sbjct: 500 ILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVR 559 Query: 206 GCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDA 265 GCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDA Sbjct: 560 GCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDA 619 Query: 266 NHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSL 325 NHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSL Sbjct: 620 NHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSL 679 Query: 326 HASSCGGRLQTS 337 HASSCGGRLQTS Sbjct: 680 HASSCGGRLQTS 691 >5HZV-A nrPDB Length = 626 Score = 530 bits (1366), Expect = e-151, Method: Composition-based stats. Identities = 244/245 (99%), Positives = 245/245 (100%) Query: 337 SPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEA 396 +PAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEA Sbjct: 374 APAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEA 433 Query: 397 EDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYL 456 EDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYL Sbjct: 434 EDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYL 493 Query: 457 SPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNC 516 SPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNC Sbjct: 494 SPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNC 553 Query: 517 VSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIIS 576 VSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIIS Sbjct: 554 VSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIIS 613 Query: 577 PDLSG 581 PDLSG Sbjct: 614 PDLSG 618 >4S38-A nrPDB Length = 406 Score = 33.5 bits (75), Expect = 0.27, Method: Composition-based stats. Identities = 32/111 (28%), Positives = 45/111 (40%), Gaps = 14/111 (12%) Query: 133 VNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGR 192 V+ T P P+T+ +E A E I A L + + S E ++++ R Sbjct: 261 VSLTPAPGEPRTKEVEVAQE---ILQALGLRAFAPEVTSCPGCGRTTSTFFQELAEEVSR 317 Query: 193 TL-----EWRPRTPAL------VRGCHLEGVAGHKEAHILRVLPGHSAGPR 232 L EWR R P + V GC + G K AHI LPG P+ Sbjct: 318 RLKERLPEWRARYPGVEELKVAVMGCVVNGPGESKHAHIGISLPGAGEEPK 368 >5VMT-A nrPDB Length = 342 Score = 31.2 bits (69), Expect = 1.6, Method: Composition-based stats. Identities = 22/79 (27%), Positives = 35/79 (44%) Query: 563 EVHRTVFMRLNIISPDLSGCTSKGLVLPAVLGITFGAFLIGALLTAALWYIYSHTRSPSK 622 ++ R LNI+ + GLV+P + G G+ + T +L + S P+ Sbjct: 203 DLRRARAAALNIVPNSTGAAKAIGLVIPELNGKLDGSAQRVPVATGSLTELVSVLERPAT 262 Query: 623 REPVVAVAAPASSESSSTN 641 +E + A ASSES N Sbjct: 263 KEEINAAMKAASSESYGYN 281 >2WOP-A nrPDB Length = 562 Score = 29.6 bits (65), Expect = 4.9, Method: Composition-based stats. Identities = 32/106 (30%), Positives = 46/106 (43%), Gaps = 11/106 (10%) Query: 432 SSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLD 491 SS P R H D+L L L +PH+ +A++ I P V++ + V +L Sbjct: 211 SSGPYRVARH--EPDTL---LHLERNPHWDRATDPIRPALPDRVELTIGLDVDVLDARLI 265 Query: 492 SCHLDLGPEG-GTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHF 536 + D+ EG G Q RA + S +PR SF LHF Sbjct: 266 AGEFDINLEGRGLQHAAQRRATADEVLR----SHTDNPRTSF-LHF 306 >3CLL-A nrPDB Length = 300 Score = 28.9 bits (63), Expect = 6.6, Method: Composition-based stats. Identities = 15/37 (40%), Positives = 24/37 (64%), Gaps = 4/37 (10%) Query: 510 RAAKGNCVSLLSPSPEGDPRFS-FLLHFYTVPIPKTG 545 R+A+ + V +L+P P G F+ F++H T+PI TG Sbjct: 206 RSARDSHVPILAPLPIG---FAVFMVHLATIPITGTG 239 >5EIY-B nrPDB Length = 729 Score = 28.9 bits (63), Expect = 6.9, Method: Composition-based stats. Identities = 49/243 (20%), Positives = 83/243 (34%), Gaps = 39/243 (16%) Query: 149 WAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCH 208 W P+ A++ L RL Q +F + ++ +G TL R+ + Sbjct: 59 WIIPLRPLAETAQVGP----LFRLQGQQARAAFRLFLPTEAVGGTLTLAQRSSIDILPES 114 Query: 209 LEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHN 268 + + + I R P VT+ + + GD I+A H Sbjct: 115 SQIIVRMNDQEIGRFTPRQFGALGAVTMPLGEAVRAGDNLVT------------IEAQHR 162 Query: 269 MQIWTTGEYSFKIFPEKNIR--GFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLH 326 +I+ + F ++ E ++ G LP A+ P + I +L Sbjct: 163 HRIYCGADAEFDLWTEVDLSQSGVALP-----------------AAAIGTEPTSFIAALT 205 Query: 327 ASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITG 386 A + GR P I+T P D + L + D+A+ L L K A T Sbjct: 206 AQAESGR----PVEIRTPTPPDEATLRTLAQALGRPLPDEALPLALSKPWSAETGPTYAR 261 Query: 387 LTF 389 +T Sbjct: 262 ITL 264 >3DUZ-A nrPDB Length = 487 Score = 28.9 bits (63), Expect = 7.8, Method: Composition-based stats. Identities = 11/38 (28%), Positives = 23/38 (60%), Gaps = 3/38 (7%) Query: 327 ASSCGGRLQTSPAPIQT---TPPKDTCSPELLMSLIQT 361 A C +++T P I+ TPPK+T ++ +++++T Sbjct: 1 AEHCNAQMKTGPYKIKNLDITPPKETLQKDVEITIVET 38 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.134 0.408 Lambda K H 0.267 0.0414 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 26,419,156 Number of Sequences: 36641 Number of extensions: 1108250 Number of successful extensions: 2291 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 2283 Number of HSP's gapped (non-prelim): 21 length of query: 658 length of database: 10,336,785 effective HSP length: 103 effective length of query: 555 effective length of database: 6,562,762 effective search space: 3642332910 effective search space used: 3642332910 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 62 (28.5 bits)