BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P16687 (354 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4XB6-C nrPDB 720 0.0 3J9M-AO nrPDB 31 1.0 2PV3-A nrPDB 30 1.5 1M5Y-A nrPDB 30 1.8 4DIM-A nrPDB 28 4.8 >4XB6-C nrPDB Length = 354 Score = 720 bits (1859), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/354 (99%), Positives = 353/354 (99%) Query: 1 MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA 60 MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA Sbjct: 1 MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA 60 Query: 61 ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLERRISAVYKDIPGGQLLGP 120 ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLERRISAVYKDIPGGQLLGP Sbjct: 61 ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLERRISAVYKDIPGGQLLGP 120 Query: 121 TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLAKFEEDSGAQPDDITR 180 TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLAKFEEDSGAQPDDITR Sbjct: 121 TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLAKFEEDSGAQPDDITR 180 Query: 181 TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG 240 TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG Sbjct: 181 TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG 240 Query: 241 FAVNVGELLMTECEMVNGFIDPPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG 300 FAVNVGELLMTECEMVNGFIDPPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG Sbjct: 241 FAVNVGELLMTECEMVNGFIDPPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG 300 Query: 301 EHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVDFQAELELLKRLQQEQNHG 354 EHATGPAQDEEFVLAHADNVE AGFVSHLKLPHYVDFQAELELLKRLQQEQNHG Sbjct: 301 EHATGPAQDEEFVLAHADNVEVAGFVSHLKLPHYVDFQAELELLKRLQQEQNHG 354 >3J9M-AO nrPDB Length = 258 Score = 30.8 bits (68), Expect = 1.0, Method: Compositional matrix adjust. Identities = 24/76 (31%), Positives = 34/76 (44%), Gaps = 11/76 (14%) Query: 127 RLLDFT-----LLANGEAPTLTTAD------SEQQPSPHVFSLLARQGLAKFEEDSGAQP 175 R LDF+ + A APTL + D + +QP S L R +E+SG P Sbjct: 173 RDLDFSTSHGAVSATPPAPTLVSGDPWYPWYNWKQPPERELSRLRRLYQGHLQEESGPPP 232 Query: 176 DDITRTPPVYPCSRSS 191 + + + PP P SS Sbjct: 233 ESMPKMPPRTPAEASS 248 >2PV3-A nrPDB Length = 299 Score = 30.0 bits (66), Expect = 1.5, Method: Compositional matrix adjust. Identities = 27/110 (24%), Positives = 50/110 (45%), Gaps = 13/110 (11%) Query: 207 LAYSTQRGYGRNHPFAGEIRSGYID--VSIVPEEL-GFAVNVGEL--LMTECEMVNGFID 261 L Y+T R R E+R+ + ++I+P+E+ A VG TE + + I Sbjct: 103 LNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESLAQQVGNQNDASTELNLSHILIP 162 Query: 262 PPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEE 311 P P ++E ++ A A+VD+A ++G+ A + D++ Sbjct: 163 LPENPTSDQ--------VNEAESQARAIVDQARNGADFGKLAIAHSADQQ 204 >1M5Y-A nrPDB Length = 408 Score = 29.6 bits (65), Expect = 1.8, Method: Compositional matrix adjust. Identities = 27/110 (24%), Positives = 50/110 (45%), Gaps = 13/110 (11%) Query: 207 LAYSTQRGYGRNHPFAGEIRSGYID--VSIVPEEL-GFAVNVGEL--LMTECEMVNGFID 261 L Y+T R R E+R+ + ++I+P+E+ A VG TE + + I Sbjct: 103 LNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESLAQQVGNQNDASTELNLSHILIP 162 Query: 262 PPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEE 311 P P ++E ++ A A+VD+A ++G+ A + D++ Sbjct: 163 LPENPTSDQ--------VNEAESQARAIVDQARNGADFGKLAIAHSADQQ 204 >4DIM-A nrPDB Length = 403 Score = 28.5 bits (62), Expect = 4.8, Method: Compositional matrix adjust. Identities = 18/81 (22%), Positives = 38/81 (46%), Gaps = 9/81 (11%) Query: 272 GYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKL 331 G+ +++R +V+ ++ E+G A D FV+ H D ++SH + Sbjct: 172 GFNETMNLTKRD---YCIVEEFIEGYEFGAQAFVYKNDVLFVMPHGDET----YMSHTAV 224 Query: 332 P--HYVDFQAELELLKRLQQE 350 P HYV + +++++ + E Sbjct: 225 PVGHYVPLDVKDDIIEKTKTE 245 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4XB6-C nrPDB 713 0.0 Sequences not found previously or not previously below threshold: 3J9M-AO nrPDB 35 0.047 6NEQ-p nrPDB 31 0.88 6GAZ-Ap nrPDB 30 1.7 4DIM-A nrPDB 30 1.8 2PV3-A nrPDB 29 4.1 1M5Y-A nrPDB 28 4.2 CONVERGED! >4XB6-C nrPDB Length = 354 Score = 713 bits (1840), Expect = 0.0, Method: Composition-based stats. Identities = 353/354 (99%), Positives = 353/354 (99%) Query: 1 MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA 60 MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA Sbjct: 1 MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA 60 Query: 61 ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLERRISAVYKDIPGGQLLGP 120 ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLERRISAVYKDIPGGQLLGP Sbjct: 61 ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLERRISAVYKDIPGGQLLGP 120 Query: 121 TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLAKFEEDSGAQPDDITR 180 TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLAKFEEDSGAQPDDITR Sbjct: 121 TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLAKFEEDSGAQPDDITR 180 Query: 181 TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG 240 TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG Sbjct: 181 TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG 240 Query: 241 FAVNVGELLMTECEMVNGFIDPPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG 300 FAVNVGELLMTECEMVNGFIDPPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG Sbjct: 241 FAVNVGELLMTECEMVNGFIDPPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG 300 Query: 301 EHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVDFQAELELLKRLQQEQNHG 354 EHATGPAQDEEFVLAHADNVE AGFVSHLKLPHYVDFQAELELLKRLQQEQNHG Sbjct: 301 EHATGPAQDEEFVLAHADNVEVAGFVSHLKLPHYVDFQAELELLKRLQQEQNHG 354 >3J9M-AO nrPDB Length = 258 Score = 35.0 bits (79), Expect = 0.047, Method: Composition-based stats. Identities = 25/79 (31%), Positives = 35/79 (44%), Gaps = 11/79 (13%) Query: 127 RLLDFT-----LLANGEAPTLTTAD------SEQQPSPHVFSLLARQGLAKFEEDSGAQP 175 R LDF+ + A APTL + D + +QP S L R +E+SG P Sbjct: 173 RDLDFSTSHGAVSATPPAPTLVSGDPWYPWYNWKQPPERELSRLRRLYQGHLQEESGPPP 232 Query: 176 DDITRTPPVYPCSRSSRLQ 194 + + + PP P SS Q Sbjct: 233 ESMPKMPPRTPAEASSTGQ 251 >6NEQ-p nrPDB Length = 258 Score = 30.7 bits (68), Expect = 0.88, Method: Composition-based stats. Identities = 24/79 (30%), Positives = 33/79 (41%), Gaps = 11/79 (13%) Query: 127 RLLDFT-----LLANGEAPTLTTAD------SEQQPSPHVFSLLARQGLAKFEEDSGAQP 175 R LDF+ + A APTL + D S +QP S L R E+SG P Sbjct: 173 RDLDFSTSHGAVSATPPAPTLVSGDPWYPWYSWKQPPERELSRLRRLYQGHLREESGPPP 232 Query: 176 DDITRTPPVYPCSRSSRLQ 194 + + + P P +S Q Sbjct: 233 ESMPKVPLTAPNEATSTEQ 251 >6GAZ-Ap nrPDB Length = 258 Score = 30.0 bits (66), Expect = 1.7, Method: Composition-based stats. Identities = 23/76 (30%), Positives = 32/76 (42%), Gaps = 11/76 (14%) Query: 127 RLLDFT-----LLANGEAPTLTTAD------SEQQPSPHVFSLLARQGLAKFEEDSGAQP 175 R LDF+ + + APTL + D S +QP S L R + E+SG P Sbjct: 173 RDLDFSTTHGAVSSTPPAPTLVSGDPWYPWYSWKQPPERELSRLRRLYQGRLREESGPPP 232 Query: 176 DDITRTPPVYPCSRSS 191 + + P P SS Sbjct: 233 ELMPEVPLTAPAEASS 248 >4DIM-A nrPDB Length = 403 Score = 30.0 bits (66), Expect = 1.8, Method: Composition-based stats. Identities = 16/64 (25%), Positives = 32/64 (50%), Gaps = 6/64 (9%) Query: 289 LVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLP--HYVDFQAELELLKR 346 +V+ ++ E+G A D FV+ H D ++SH +P HYV + +++++ Sbjct: 186 IVEEFIEGYEFGAQAFVYKNDVLFVMPHGDET----YMSHTAVPVGHYVPLDVKDDIIEK 241 Query: 347 LQQE 350 + E Sbjct: 242 TKTE 245 >2PV3-A nrPDB Length = 299 Score = 28.8 bits (63), Expect = 4.1, Method: Composition-based stats. Identities = 27/111 (24%), Positives = 50/111 (45%), Gaps = 13/111 (11%) Query: 206 ALAYSTQRGYGRNHPFAGEIRSGYID--VSIVPEEL-GFAVNVGEL--LMTECEMVNGFI 260 L Y+T R R E+R+ + ++I+P+E+ A VG TE + + I Sbjct: 102 GLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESLAQQVGNQNDASTELNLSHILI 161 Query: 261 DPPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEE 311 P P ++E ++ A A+VD+A ++G+ A + D++ Sbjct: 162 PLPENPTSDQ--------VNEAESQARAIVDQARNGADFGKLAIAHSADQQ 204 >1M5Y-A nrPDB Length = 408 Score = 28.4 bits (62), Expect = 4.2, Method: Composition-based stats. Identities = 27/111 (24%), Positives = 50/111 (45%), Gaps = 13/111 (11%) Query: 206 ALAYSTQRGYGRNHPFAGEIRSGYID--VSIVPEEL-GFAVNVGEL--LMTECEMVNGFI 260 L Y+T R R E+R+ + ++I+P+E+ A VG TE + + I Sbjct: 102 GLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESLAQQVGNQNDASTELNLSHILI 161 Query: 261 DPPGEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEE 311 P P ++E ++ A A+VD+A ++G+ A + D++ Sbjct: 162 PLPENPTSDQ--------VNEAESQARAIVDQARNGADFGKLAIAHSADQQ 204 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.141 0.428 Lambda K H 0.267 0.0411 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,361,362 Number of Sequences: 36641 Number of extensions: 593143 Number of successful extensions: 1252 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 1242 Number of HSP's gapped (non-prelim): 19 length of query: 354 length of database: 10,336,785 effective HSP length: 98 effective length of query: 256 effective length of database: 6,745,967 effective search space: 1726967552 effective search space used: 1726967552 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.2 bits) S2: 60 (27.7 bits)