BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P16686 (194 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4XB6-B nrPDB 390 e-109 3UJZ-A nrPDB 33 0.058 2B3Y-A nrPDB 28 3.6 3SN2-A nrPDB 27 3.9 5DZV-A nrPDB 27 4.4 1FDR-A nrPDB 27 5.3 >4XB6-B nrPDB Length = 194 Score = 390 bits (1001), Expect = e-109, Method: Compositional matrix adjust. Identities = 193/194 (99%), Positives = 194/194 (100%) Query: 1 MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPLNIATTSVLLTLADNDT 60 MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPLNIATTSVLLTLADNDT Sbjct: 1 MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPLNIATTSVLLTLADNDT 60 Query: 61 PVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAISSEQLNALSTGTAVAPEAG 120 PVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAISSEQLNALSTGTAVAPEAG Sbjct: 61 PVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAISSEQLNALSTGTAVAPEAG 120 Query: 121 ATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHELTERPHPFPLGIDLILTCGE 180 ATLILQVASLSGGRMLRLTGAGIAEERMIAP+LPECILHELTERPHPFPLGIDLILTCGE Sbjct: 121 ATLILQVASLSGGRMLRLTGAGIAEERMIAPRLPECILHELTERPHPFPLGIDLILTCGE 180 Query: 181 RLLAIPRTTHVEVC 194 RLLAIPRTTHVEVC Sbjct: 181 RLLAIPRTTHVEVC 194 >3UJZ-A nrPDB Length = 869 Score = 33.5 bits (75), Expect = 0.058, Method: Composition-based stats. Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 15/70 (21%) Query: 55 LADNDTPVWLSTP------------LNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS 102 L+DND + + T NN ++N +FH N P SQP QAT ++ + Sbjct: 695 LSDNDCQLQVDTKEGQLRFRLANHRANNTVMN---KFHINVPTESQPTQATLVCNNKILD 751 Query: 103 SEQLNALSTG 112 ++ L G Sbjct: 752 TKSLTPAPEG 761 >2B3Y-A nrPDB Length = 888 Score = 27.7 bits (60), Expect = 3.6, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 19/34 (55%) Query: 149 IAPQLPECILHELTERPHPFPLGIDLILTCGERL 182 I+ LP+ I + L +PHP D++LT + L Sbjct: 234 ISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHL 267 >3SN2-A nrPDB Length = 908 Score = 27.3 bits (59), Expect = 3.9, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 19/34 (55%) Query: 149 IAPQLPECILHELTERPHPFPLGIDLILTCGERL 182 I+ LP+ I + L +PHP D++LT + L Sbjct: 254 ISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHL 287 >5DZV-A nrPDB Length = 539 Score = 27.3 bits (59), Expect = 4.4, Method: Compositional matrix adjust. Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 5/50 (10%) Query: 46 IATTSVLLTLADNDTP-VWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATF 94 IA V++T D +P +W + ++ ++ + + NAPL +QPE F Sbjct: 389 IANYDVIVTARDGGSPSLWATASVSVEVAD----VNDNAPLFAQPEYTVF 434 >1FDR-A nrPDB Length = 248 Score = 26.9 bits (58), Expect = 5.3, Method: Compositional matrix adjust. Identities = 14/28 (50%), Positives = 16/28 (57%) Query: 90 EQATFAVTDEAISSEQLNALSTGTAVAP 117 E A F V DE E L L+TGTA+ P Sbjct: 93 EAAGFFVLDEVPHCETLWMLATGTAIGP 120 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4XB6-B nrPDB 368 e-103 Sequences not found previously or not previously below threshold: 3UJZ-A nrPDB 34 0.032 2B3Y-A nrPDB 28 2.2 3SN2-A nrPDB 28 2.3 5DZV-A nrPDB 28 2.4 1FDR-A nrPDB 27 3.8 4IPV-A nrPDB 27 5.9 1V4V-A nrPDB 27 6.9 3N6R-A nrPDB 26 8.8 CONVERGED! >4XB6-B nrPDB Length = 194 Score = 368 bits (945), Expect = e-103, Method: Composition-based stats. Identities = 193/194 (99%), Positives = 194/194 (100%) Query: 1 MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPLNIATTSVLLTLADNDT 60 MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPLNIATTSVLLTLADNDT Sbjct: 1 MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPLNIATTSVLLTLADNDT 60 Query: 61 PVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAISSEQLNALSTGTAVAPEAG 120 PVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAISSEQLNALSTGTAVAPEAG Sbjct: 61 PVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAISSEQLNALSTGTAVAPEAG 120 Query: 121 ATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHELTERPHPFPLGIDLILTCGE 180 ATLILQVASLSGGRMLRLTGAGIAEERMIAP+LPECILHELTERPHPFPLGIDLILTCGE Sbjct: 121 ATLILQVASLSGGRMLRLTGAGIAEERMIAPRLPECILHELTERPHPFPLGIDLILTCGE 180 Query: 181 RLLAIPRTTHVEVC 194 RLLAIPRTTHVEVC Sbjct: 181 RLLAIPRTTHVEVC 194 >3UJZ-A nrPDB Length = 869 Score = 34.2 bits (77), Expect = 0.032, Method: Composition-based stats. Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 15/70 (21%) Query: 55 LADNDTPVWLSTP------------LNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS 102 L+DND + + T NN ++N +FH N P SQP QAT ++ + Sbjct: 695 LSDNDCQLQVDTKEGQLRFRLANHRANNTVMN---KFHINVPTESQPTQATLVCNNKILD 751 Query: 103 SEQLNALSTG 112 ++ L G Sbjct: 752 TKSLTPAPEG 761 >2B3Y-A nrPDB Length = 888 Score = 28.4 bits (62), Expect = 2.2, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 19/34 (55%) Query: 149 IAPQLPECILHELTERPHPFPLGIDLILTCGERL 182 I+ LP+ I + L +PHP D++LT + L Sbjct: 234 ISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHL 267 >3SN2-A nrPDB Length = 908 Score = 28.1 bits (61), Expect = 2.3, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 19/34 (55%) Query: 149 IAPQLPECILHELTERPHPFPLGIDLILTCGERL 182 I+ LP+ I + L +PHP D++LT + L Sbjct: 254 ISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHL 287 >5DZV-A nrPDB Length = 539 Score = 28.1 bits (61), Expect = 2.4, Method: Composition-based stats. Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 5/50 (10%) Query: 46 IATTSVLLTLADNDTP-VWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATF 94 IA V++T D +P +W + ++ ++ + + NAPL +QPE F Sbjct: 389 IANYDVIVTARDGGSPSLWATASVSVEVAD----VNDNAPLFAQPEYTVF 434 >1FDR-A nrPDB Length = 248 Score = 27.3 bits (59), Expect = 3.8, Method: Composition-based stats. Identities = 14/28 (50%), Positives = 16/28 (57%) Query: 90 EQATFAVTDEAISSEQLNALSTGTAVAP 117 E A F V DE E L L+TGTA+ P Sbjct: 93 EAAGFFVLDEVPHCETLWMLATGTAIGP 120 >4IPV-A nrPDB Length = 137 Score = 26.9 bits (58), Expect = 5.9, Method: Composition-based stats. Identities = 16/52 (30%), Positives = 22/52 (42%) Query: 91 QATFAVTDEAISSEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAG 142 +A E + E L + GT V P I Q GGR+L ++ AG Sbjct: 71 RAATLTAKEKSAVEHLTFTANGTLVPPTERTFAICQNGKTDGGRVLSISQAG 122 >1V4V-A nrPDB Length = 376 Score = 26.5 bits (57), Expect = 6.9, Method: Composition-based stats. Identities = 15/46 (32%), Positives = 24/46 (52%) Query: 127 VASLSGGRMLRLTGAGIAEERMIAPQLPECILHELTERPHPFPLGI 172 +A+L +L +T +G +E A +P +L +TERP GI Sbjct: 268 MAALMRASLLLVTDSGGLQEEGAALGVPVVVLRNVTERPEGLKAGI 313 >3N6R-A nrPDB Length = 681 Score = 26.1 bits (56), Expect = 8.8, Method: Composition-based stats. Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 7/58 (12%) Query: 86 VSQPEQATFAVTDEAISSEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGI 143 VS + ++ VT + +QL L GA L+L+V +SGG +R GA + Sbjct: 537 VSFDDGSSMRVTSDWTPGDQLANLMVD-------GAPLVLKVGKISGGFRIRTRGADL 587 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.139 0.430 Lambda K H 0.267 0.0408 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,314,896 Number of Sequences: 36641 Number of extensions: 272793 Number of successful extensions: 712 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 696 Number of HSP's gapped (non-prelim): 20 length of query: 194 length of database: 10,336,785 effective HSP length: 92 effective length of query: 102 effective length of database: 6,965,813 effective search space: 710512926 effective search space used: 710512926 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 56 (26.2 bits)