BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P16458 (385 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2I0Q-B nrPDB 771 0.0 1OTC-B nrPDB 535 e-152 1PH1-B nrPDB 456 e-128 2OTN-A nrPDB 28 6.1 >2I0Q-B nrPDB Length = 385 Score = 771 bits (1990), Expect = 0.0, Method: Compositional matrix adjust. Identities = 385/385 (100%), Positives = 385/385 (100%) Query: 1 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA 60 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA Sbjct: 1 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA 60 Query: 61 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS 120 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS Sbjct: 61 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS 120 Query: 121 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG 180 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG Sbjct: 121 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG 180 Query: 181 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV 240 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV Sbjct: 181 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV 240 Query: 241 KGGAKGKGKAAAKAAKGKKLSAKKGDSSAADVRKSVDKIVKYTPSKGSRKDTPQKSQAPA 300 KGGAKGKGKAAAKAAKGKKLSAKKGDSSAADVRKSVDKIVKYTPSKGSRKDTPQKSQAPA Sbjct: 241 KGGAKGKGKAAAKAAKGKKLSAKKGDSSAADVRKSVDKIVKYTPSKGSRKDTPQKSQAPA 300 Query: 301 AGKSSAKKGGKKAVPSAPSPSGKKSALTTDKMTMAQFVKYLDWHEKKKGGKVSSGGKVLG 360 AGKSSAKKGGKKAVPSAPSPSGKKSALTTDKMTMAQFVKYLDWHEKKKGGKVSSGGKVLG Sbjct: 301 AGKSSAKKGGKKAVPSAPSPSGKKSALTTDKMTMAQFVKYLDWHEKKKGGKVSSGGKVLG 360 Query: 361 KRSAGKASATSGKASKASKKTAAKK 385 KRSAGKASATSGKASKASKKTAAKK Sbjct: 361 KRSAGKASATSGKASKASKKTAAKK 385 >1OTC-B nrPDB Length = 260 Score = 535 bits (1378), Expect = e-152, Method: Compositional matrix adjust. Identities = 260/260 (100%), Positives = 260/260 (100%) Query: 1 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA 60 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA Sbjct: 1 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA 60 Query: 61 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS 120 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS Sbjct: 61 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS 120 Query: 121 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG 180 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG Sbjct: 121 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG 180 Query: 181 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV 240 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV Sbjct: 181 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV 240 Query: 241 KGGAKGKGKAAAKAAKGKKL 260 KGGAKGKGKAAAKAAKGKKL Sbjct: 241 KGGAKGKGKAAAKAAKGKKL 260 >1PH1-B nrPDB Length = 217 Score = 456 bits (1172), Expect = e-128, Method: Compositional matrix adjust. Identities = 217/217 (100%), Positives = 217/217 (100%) Query: 8 PQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVAPYFTKEA 67 PQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVAPYFTKEA Sbjct: 1 PQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVAPYFTKEA 60 Query: 68 VNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHSFKPNLQE 127 VNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHSFKPNLQE Sbjct: 61 VNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHSFKPNLQE 120 Query: 128 RLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAGKKGKVDA 187 RLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAGKKGKVDA Sbjct: 121 RLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAGKKGKVDA 180 Query: 188 GIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKG 224 GIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKG Sbjct: 181 GIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKG 217 >2OTN-A nrPDB Length = 308 Score = 28.1 bits (61), Expect = 6.1, Method: Compositional matrix adjust. Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 3/39 (7%) Query: 21 LFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFV 59 +FNN+G K N L+C K +Y H LV D F + Sbjct: 85 MFNNDGSEGKSCGN---GLRCVAKYAYEHKLVEDTVFTI 120 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2I0Q-B nrPDB 699 0.0 1OTC-B nrPDB 525 e-149 1PH1-B nrPDB 471 e-133 Sequences not found previously or not previously below threshold: 1IOK-A nrPDB 34 0.16 2OTN-A nrPDB 30 2.0 CONVERGED! >2I0Q-B nrPDB Length = 385 Score = 699 bits (1805), Expect = 0.0, Method: Composition-based stats. Identities = 385/385 (100%), Positives = 385/385 (100%) Query: 1 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA 60 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA Sbjct: 1 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA 60 Query: 61 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS 120 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS Sbjct: 61 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS 120 Query: 121 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG 180 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG Sbjct: 121 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG 180 Query: 181 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV 240 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV Sbjct: 181 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV 240 Query: 241 KGGAKGKGKAAAKAAKGKKLSAKKGDSSAADVRKSVDKIVKYTPSKGSRKDTPQKSQAPA 300 KGGAKGKGKAAAKAAKGKKLSAKKGDSSAADVRKSVDKIVKYTPSKGSRKDTPQKSQAPA Sbjct: 241 KGGAKGKGKAAAKAAKGKKLSAKKGDSSAADVRKSVDKIVKYTPSKGSRKDTPQKSQAPA 300 Query: 301 AGKSSAKKGGKKAVPSAPSPSGKKSALTTDKMTMAQFVKYLDWHEKKKGGKVSSGGKVLG 360 AGKSSAKKGGKKAVPSAPSPSGKKSALTTDKMTMAQFVKYLDWHEKKKGGKVSSGGKVLG Sbjct: 301 AGKSSAKKGGKKAVPSAPSPSGKKSALTTDKMTMAQFVKYLDWHEKKKGGKVSSGGKVLG 360 Query: 361 KRSAGKASATSGKASKASKKTAAKK 385 KRSAGKASATSGKASKASKKTAAKK Sbjct: 361 KRSAGKASATSGKASKASKKTAAKK 385 >1OTC-B nrPDB Length = 260 Score = 525 bits (1353), Expect = e-149, Method: Composition-based stats. Identities = 260/260 (100%), Positives = 260/260 (100%) Query: 1 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA 60 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA Sbjct: 1 MSKGASAPQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVA 60 Query: 61 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS 120 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS Sbjct: 61 PYFTKEAVNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHS 120 Query: 121 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG 180 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG Sbjct: 121 FKPNLQERLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAG 180 Query: 181 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV 240 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV Sbjct: 181 KKGKVDAGIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKGKDALNKAADHTDGAKV 240 Query: 241 KGGAKGKGKAAAKAAKGKKL 260 KGGAKGKGKAAAKAAKGKKL Sbjct: 241 KGGAKGKGKAAAKAAKGKKL 260 >1PH1-B nrPDB Length = 217 Score = 471 bits (1211), Expect = e-133, Method: Composition-based stats. Identities = 217/217 (100%), Positives = 217/217 (100%) Query: 8 PQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVAPYFTKEA 67 PQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVAPYFTKEA Sbjct: 1 PQQQSAFKQLYTELFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFVAPYFTKEA 60 Query: 68 VNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHSFKPNLQE 127 VNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHSFKPNLQE Sbjct: 61 VNEFHAKFPNVNIVDLTDKVIVINNWSLELRRVNSAEVFTSYANLEARLIVHSFKPNLQE 120 Query: 128 RLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAGKKGKVDA 187 RLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAGKKGKVDA Sbjct: 121 RLNPTRYPVNLFRDDEFKTTIQHFRHTALQAAINKTVKGDNLVDISKVADAAGKKGKVDA 180 Query: 188 GIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKG 224 GIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKG Sbjct: 181 GIVKASASKGDEFSDFSFKEGNTATLKIADIFVQEKG 217 >1IOK-A nrPDB Length = 545 Score = 33.6 bits (75), Expect = 0.16, Method: Composition-based stats. Identities = 41/172 (23%), Positives = 73/172 (42%), Gaps = 38/172 (22%) Query: 155 ALQAAINKTVKGDNLVDISKVADAAGKKGKVDAGIVKASASKGDEFSDFSFKE------- 207 A + +INK D + + D AG+K +++A + + + SD+ ++ Sbjct: 320 AKKVSINK--------DNTTIVDGAGEKAEIEARVSQIRQQIEETTSDYDREKLQERVAK 371 Query: 208 --GNTATLKIA---DIFVQEKGKDALNKAADHTDGAKVKGGAKGKGKAAAKAAKG-KKLS 261 G A +++ +I V+E+ KD ++ A + T A +G G G A + AK + LS Sbjct: 372 LAGGVAVIRVGGMTEIEVKER-KDRVDDALNATRAAVQEGIVVGGGVALVQGAKVLEGLS 430 Query: 262 AKKGDSSA--ADVRKSVDKIVKYTPSKGSRKDTPQKSQAPAAGKSSAKKGGK 311 D A A +R+++ + P + A AG A GK Sbjct: 431 GANSDQDAGIAIIRRAL--------------EAPMRQIAENAGVDGAVVAGK 468 >2OTN-A nrPDB Length = 308 Score = 29.7 bits (65), Expect = 2.0, Method: Composition-based stats. Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 3/39 (7%) Query: 21 LFNNEGDFSKVSSNLKKPLKCYVKESYPHFLVTDGYFFV 59 +FNN+G K N L+C K +Y H LV D F + Sbjct: 85 MFNNDGSEGKSCGN---GLRCVAKYAYEHKLVEDTVFTI 120 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.310 0.126 0.346 Lambda K H 0.267 0.0396 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,178,925 Number of Sequences: 36641 Number of extensions: 572258 Number of successful extensions: 1354 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 39 Number of HSP's that attempted gapping in prelim test: 1285 Number of HSP's gapped (non-prelim): 91 length of query: 385 length of database: 10,336,785 effective HSP length: 99 effective length of query: 286 effective length of database: 6,709,326 effective search space: 1918867236 effective search space used: 1918867236 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 60 (27.8 bits)