BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P15382
         (129 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

2K21-A nrPDB                                                          269   3e-73
2M0Q-A nrPDB                                                           55   8e-09
2NDJ-A nrPDB                                                           37   0.002
4HTF-A nrPDB                                                           27   3.2  
3R3L-A nrPDB                                                           26   4.4  
3CRV-A nrPDB                                                           26   5.0  
4XGC-B nrPDB                                                           25   7.2  
3MWP-A nrPDB                                                           25   8.8  
5YZG-3 nrPDB                                                           25   9.1  

>2K21-A nrPDB
          Length = 138

 Score =  269 bits (687), Expect = 3e-73,   Method: Compositional matrix adjust.
 Identities = 127/129 (98%), Positives = 128/129 (99%)

Query: 1   MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRSSDGKLEALYVLMVLGFFGFFTLG 60
           MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRS DGKLEALYVLMVLGFFGFFTLG
Sbjct: 10  MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRSGDGKLEALYVLMVLGFFGFFTLG 69

Query: 61  IMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESYRSCYVVENHLAIEQPNT 120
           IMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESY+SCYVVENHLAIEQPNT
Sbjct: 70  IMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESYKSCYVVENHLAIEQPNT 129

Query: 121 HLPETKPSP 129
           HLPETKPSP
Sbjct: 130 HLPETKPSP 138


>2M0Q-A nrPDB
          Length = 123

 Score = 55.1 bits (131), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 23/51 (45%), Positives = 38/51 (74%), Gaps = 1/51 (1%)

Query: 45  LYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYV 95
           LY+++++G F F  + I++S ++SK+ EHSNDP++ YI  D WQEK K+ +
Sbjct: 51  LYLMVMIGMFSFIIVAILVSTVKSKRREHSNDPYHQYIVED-WQEKYKSQI 100


>2NDJ-A nrPDB
          Length = 112

 Score = 37.0 bits (84), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 14/40 (35%), Positives = 27/40 (67%)

Query: 45  LYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIES 84
           +Y+L V+  F      ++L Y RS+K++  +DP++VYI++
Sbjct: 68  MYILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKN 107


>4HTF-A nrPDB
          Length = 285

 Score = 26.6 bits (57), Expect = 3.2,   Method: Compositional matrix adjust.
 Identities = 21/68 (30%), Positives = 27/68 (39%), Gaps = 11/68 (16%)

Query: 49  MVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVY--IESDAWQEKDKAYVQARVLESY--- 103
           MV G F +   G+     R+   ++  DP  VY  +E   WQ   K  V  RV   Y   
Sbjct: 184 MVAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGV--RVFHDYLRE 241

Query: 104 ----RSCY 107
               R CY
Sbjct: 242 KHQQRDCY 249


>3R3L-A nrPDB
          Length = 582

 Score = 26.2 bits (56), Expect = 4.4,   Method: Compositional matrix adjust.
 Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 9/65 (13%)

Query: 24  GGNMSGLARRSPRSSDGKLEA-LYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYI 82
           GGNM    + SP+S DG L++ L V   LG F   T G        ++  + N  + + +
Sbjct: 261 GGNMLETIKVSPQSMDGILKSILKVKRALGMFVSDTPG--------ERNPYENILYKICL 312

Query: 83  ESDAW 87
             D W
Sbjct: 313 SGDGW 317


>3CRV-A nrPDB
          Length = 551

 Score = 25.8 bits (55), Expect = 5.0,   Method: Composition-based stats.
 Identities = 12/50 (24%), Positives = 24/50 (48%)

Query: 33  RSPRSSDGKLEALYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYI 82
           R      GK+  +++  +L FF   ++G  + +  SK+L   N   + Y+
Sbjct: 259 RKDSLKQGKVNKIHIGSILRFFSLLSIGSFIPFSYSKRLVIKNPEISYYL 308


>4XGC-B nrPDB
          Length = 354

 Score = 25.4 bits (54), Expect = 7.2,   Method: Compositional matrix adjust.
 Identities = 23/79 (29%), Positives = 34/79 (43%), Gaps = 5/79 (6%)

Query: 17  WQETVQQGGN--MSGLARRSPRSSDGKLEALY---VLMVLGFFGFFTLGIMLSYIRSKKL 71
           W   + +G N  + GL  +         E L+   VL+V GFF   T+  ML  I S  L
Sbjct: 79  WMCILNEGFNILLYGLGSKHQLLQSFHREVLHKQTVLVVNGFFPSLTIKDMLDSITSDIL 138

Query: 72  EHSNDPFNVYIESDAWQEK 90
           +    P N +   D  +E+
Sbjct: 139 DAGISPANPHEAVDMIEEE 157


>3MWP-A nrPDB
          Length = 577

 Score = 25.0 bits (53), Expect = 8.8,   Method: Compositional matrix adjust.
 Identities = 17/64 (26%), Positives = 30/64 (46%), Gaps = 7/64 (10%)

Query: 24  GGNMSGLARRSPRSSDGKLEALYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIE 83
           GGNM    + SP++ DG L++  +L V    G F     +S    ++  + N  + + + 
Sbjct: 269 GGNMLETIKVSPQTMDGILKS--ILKVKKALGMF-----ISDTPGERNPYENILYKICLS 321

Query: 84  SDAW 87
            D W
Sbjct: 322 GDGW 325


>5YZG-3 nrPDB
          Length = 754

 Score = 25.0 bits (53), Expect = 9.1,   Method: Composition-based stats.
 Identities = 16/58 (27%), Positives = 25/58 (43%)

Query: 67  RSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESYRSCYVVENHLAIEQPNTHLPE 124
           R KK +    PF+   +S    E   + ++ +  E  RS    EN  +  Q N H+ E
Sbjct: 678 REKKADRDQSPFSKIKQSSQDNELKSSMLKNKEDEKIRSSVEKENQKSKGQENDHVHE 735


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

2K21-A nrPDB                                                          266   2e-72
2M0Q-A nrPDB                                                           88   1e-18

Sequences not found previously or not previously below threshold:

2NDJ-A nrPDB                                                           41   2e-04
3R3L-A nrPDB                                                           30   0.26 
3MWP-A nrPDB                                                           29   0.61 
5L22-B nrPDB                                                           26   5.2  

>2K21-A nrPDB
          Length = 138

 Score =  266 bits (680), Expect = 2e-72,   Method: Composition-based stats.
 Identities = 127/129 (98%), Positives = 128/129 (99%)

Query: 1   MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRSSDGKLEALYVLMVLGFFGFFTLG 60
           MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRS DGKLEALYVLMVLGFFGFFTLG
Sbjct: 10  MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRSGDGKLEALYVLMVLGFFGFFTLG 69

Query: 61  IMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESYRSCYVVENHLAIEQPNT 120
           IMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESY+SCYVVENHLAIEQPNT
Sbjct: 70  IMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESYKSCYVVENHLAIEQPNT 129

Query: 121 HLPETKPSP 129
           HLPETKPSP
Sbjct: 130 HLPETKPSP 138


>2M0Q-A nrPDB
          Length = 123

 Score = 87.8 bits (216), Expect = 1e-18,   Method: Composition-based stats.
 Identities = 23/51 (45%), Positives = 38/51 (74%), Gaps = 1/51 (1%)

Query: 45  LYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYV 95
           LY+++++G F F  + I++S ++SK+ EHSNDP++ YI  D WQEK K+ +
Sbjct: 51  LYLMVMIGMFSFIIVAILVSTVKSKRREHSNDPYHQYIVED-WQEKYKSQI 100


>2NDJ-A nrPDB
          Length = 112

 Score = 40.8 bits (94), Expect = 2e-04,   Method: Composition-based stats.
 Identities = 16/53 (30%), Positives = 30/53 (56%)

Query: 32  RRSPRSSDGKLEALYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIES 84
           RR+          +Y+L V+  F      ++L Y RS+K++  +DP++VYI++
Sbjct: 55  RRASLPGRDDNSYMYILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKN 107


>3R3L-A nrPDB
          Length = 582

 Score = 30.0 bits (66), Expect = 0.26,   Method: Composition-based stats.
 Identities = 19/65 (29%), Positives = 30/65 (46%), Gaps = 9/65 (13%)

Query: 24  GGNMSGLARRSPRSSDGKLEA-LYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYI 82
           GGNM    + SP+S DG L++ L V   LG F        +S    ++  + N  + + +
Sbjct: 261 GGNMLETIKVSPQSMDGILKSILKVKRALGMF--------VSDTPGERNPYENILYKICL 312

Query: 83  ESDAW 87
             D W
Sbjct: 313 SGDGW 317


>3MWP-A nrPDB
          Length = 577

 Score = 28.9 bits (63), Expect = 0.61,   Method: Composition-based stats.
 Identities = 18/65 (27%), Positives = 30/65 (46%), Gaps = 9/65 (13%)

Query: 24  GGNMSGLARRSPRSSDGKLEA-LYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYI 82
           GGNM    + SP++ DG L++ L V   LG F        +S    ++  + N  + + +
Sbjct: 269 GGNMLETIKVSPQTMDGILKSILKVKKALGMF--------ISDTPGERNPYENILYKICL 320

Query: 83  ESDAW 87
             D W
Sbjct: 321 SGDGW 325


>5L22-B nrPDB
          Length = 572

 Score = 25.8 bits (55), Expect = 5.2,   Method: Composition-based stats.
 Identities = 11/30 (36%), Positives = 16/30 (53%)

Query: 47  VLMVLGFFGFFTLGIMLSYIRSKKLEHSND 76
           V+ +L       L I+  YI  KKL+ SN+
Sbjct: 159 VMAILSMAVIVALAILNEYITKKKLKESNE 188


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.310    0.135    0.410 

Lambda     K      H
   0.267   0.0394    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,312,094
Number of Sequences: 36641
Number of extensions: 181122
Number of successful extensions: 447
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 434
Number of HSP's gapped (non-prelim): 20
length of query: 129
length of database: 10,336,785
effective HSP length: 86
effective length of query: 43
effective length of database: 7,185,659
effective search space: 308983337
effective search space used: 308983337
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.2 bits)
S2: 53 (25.1 bits)