BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P14908 (341 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1I4W-A nrPDB 705 0.0 4GC9-A nrPDB 35 0.052 2DH2-A nrPDB 30 1.6 1G6H-A nrPDB 30 1.7 6IRT-A nrPDB 30 1.8 5M99-B nrPDB 28 6.0 >1I4W-A nrPDB Length = 353 Score = 705 bits (1819), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/340 (100%), Positives = 340/340 (100%) Query: 2 SVPIPGIKDISKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKHPEELKVLDLYPGVGIQS 61 SVPIPGIKDISKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKHPEELKVLDLYPGVGIQS Sbjct: 14 SVPIPGIKDISKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKHPEELKVLDLYPGVGIQS 73 Query: 62 AIFYNKYCPRQYSLLEKRSSLYKFLNAKFEGSPLQILKRDPYDWSTYSNLIDEERIFVPE 121 AIFYNKYCPRQYSLLEKRSSLYKFLNAKFEGSPLQILKRDPYDWSTYSNLIDEERIFVPE Sbjct: 74 AIFYNKYCPRQYSLLEKRSSLYKFLNAKFEGSPLQILKRDPYDWSTYSNLIDEERIFVPE 133 Query: 122 VQSSDHINDKFLTVANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLL 181 VQSSDHINDKFLTVANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLL Sbjct: 134 VQSSDHINDKFLTVANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLL 193 Query: 182 ARPGMHSRSKCSVVREAFTDTKLIAISDANELKGFDSQCIEEWDPILFSAAEIWPTKGKP 241 ARPGMHSRSKCSVVREAFTDTKLIAISDANELKGFDSQCIEEWDPILFSAAEIWPTKGKP Sbjct: 194 ARPGMHSRSKCSVVREAFTDTKLIAISDANELKGFDSQCIEEWDPILFSAAEIWPTKGKP 253 Query: 242 IALVEMDPIDFDFDVDNWDYVTRHLMILKRTPLNTVMDSLGHGGQQYFNSRITDKDLLKK 301 IALVEMDPIDFDFDVDNWDYVTRHLMILKRTPLNTVMDSLGHGGQQYFNSRITDKDLLKK Sbjct: 254 IALVEMDPIDFDFDVDNWDYVTRHLMILKRTPLNTVMDSLGHGGQQYFNSRITDKDLLKK 313 Query: 302 CPIDLTNDEFIYLTKLFMEWPFKPDILMDFVDMYQTEHSG 341 CPIDLTNDEFIYLTKLFMEWPFKPDILMDFVDMYQTEHSG Sbjct: 314 CPIDLTNDEFIYLTKLFMEWPFKPDILMDFVDMYQTEHSG 353 >4GC9-A nrPDB Length = 345 Score = 35.0 bits (79), Expect = 0.052, Method: Compositional matrix adjust. Identities = 18/69 (26%), Positives = 34/69 (49%) Query: 135 VANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLLARPGMHSRSKCSV 194 + N+ S LI++WL I K+ + +G+ KM L A +L+A G S+ S+ Sbjct: 139 IGNLPFSVSTPLIIKWLENISLKDGPFVYGRTKMTLTFQKEVAERLVATTGSKQHSRLSI 198 Query: 195 VREAFTDTK 203 + + + + Sbjct: 199 MAQYLCNVE 207 >2DH2-A nrPDB Length = 424 Score = 30.0 bits (66), Expect = 1.6, Method: Compositional matrix adjust. Identities = 16/80 (20%), Positives = 35/80 (43%), Gaps = 14/80 (17%) Query: 104 DWSTYSNLIDEERIFVPEVQSSDH--------------INDKFLTVANVTGEGSEGLIMQ 149 +W + E+R+ + SSD + +L+ + TGE ++ L+ Q Sbjct: 156 EWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQ 215 Query: 150 WLSCIGNKNWLYRFGKVKML 169 +L+ GN+ + + ++L Sbjct: 216 YLNATGNRWCSWSLSQARLL 235 >1G6H-A nrPDB Length = 257 Score = 30.0 bits (66), Expect = 1.7, Method: Compositional matrix adjust. Identities = 22/85 (25%), Positives = 42/85 (49%), Gaps = 12/85 (14%) Query: 14 LKFFYGFKYLWNPTVYNKIFDKL---DLTKTYKHPEELK---------VLDLYPGVGIQS 61 LK G Y N + NK +L + +T++ P+ LK + ++ PG + Sbjct: 57 LKADEGRVYFENKDITNKEPAELYHYGIVRTFQTPQPLKEMTVLENLLIGEICPGESPLN 116 Query: 62 AIFYNKYCPRQYSLLEKRSSLYKFL 86 ++FY K+ P++ ++EK + +FL Sbjct: 117 SLFYKKWIPKEEEMVEKAFKILEFL 141 >6IRT-A nrPDB Length = 647 Score = 29.6 bits (65), Expect = 1.8, Method: Compositional matrix adjust. Identities = 16/80 (20%), Positives = 35/80 (43%), Gaps = 14/80 (17%) Query: 104 DWSTYSNLIDEERIFVPEVQSSDH--------------INDKFLTVANVTGEGSEGLIMQ 149 +W + E+R+ + SSD + +L+ + TGE ++ L+ Q Sbjct: 377 EWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQ 436 Query: 150 WLSCIGNKNWLYRFGKVKML 169 +L+ GN+ + + ++L Sbjct: 437 YLNATGNRWCSWSLSQARLL 456 >5M99-B nrPDB Length = 505 Score = 28.1 bits (61), Expect = 6.0, Method: Compositional matrix adjust. Identities = 11/31 (35%), Positives = 19/31 (61%) Query: 65 YNKYCPRQYSLLEKRSSLYKFLNAKFEGSPL 95 Y K+ Y ++KR++L+ FL +GSP+ Sbjct: 292 YKKFSESDYEFIKKRAALWYFLIFTLKGSPV 322 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1I4W-A nrPDB 742 0.0 Sequences not found previously or not previously below threshold: 4GC9-A nrPDB 34 0.082 1G6H-A nrPDB 32 0.46 6IRT-A nrPDB 30 1.3 2DH2-A nrPDB 30 1.3 5M99-B nrPDB 29 3.1 2WWD-A nrPDB 29 3.6 6BRH-A nrPDB 28 5.2 4G56-A nrPDB 28 7.5 5JXF-A nrPDB 28 8.7 CONVERGED! >1I4W-A nrPDB Length = 353 Score = 742 bits (1917), Expect = 0.0, Method: Composition-based stats. Identities = 340/340 (100%), Positives = 340/340 (100%) Query: 2 SVPIPGIKDISKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKHPEELKVLDLYPGVGIQS 61 SVPIPGIKDISKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKHPEELKVLDLYPGVGIQS Sbjct: 14 SVPIPGIKDISKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKHPEELKVLDLYPGVGIQS 73 Query: 62 AIFYNKYCPRQYSLLEKRSSLYKFLNAKFEGSPLQILKRDPYDWSTYSNLIDEERIFVPE 121 AIFYNKYCPRQYSLLEKRSSLYKFLNAKFEGSPLQILKRDPYDWSTYSNLIDEERIFVPE Sbjct: 74 AIFYNKYCPRQYSLLEKRSSLYKFLNAKFEGSPLQILKRDPYDWSTYSNLIDEERIFVPE 133 Query: 122 VQSSDHINDKFLTVANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLL 181 VQSSDHINDKFLTVANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLL Sbjct: 134 VQSSDHINDKFLTVANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLL 193 Query: 182 ARPGMHSRSKCSVVREAFTDTKLIAISDANELKGFDSQCIEEWDPILFSAAEIWPTKGKP 241 ARPGMHSRSKCSVVREAFTDTKLIAISDANELKGFDSQCIEEWDPILFSAAEIWPTKGKP Sbjct: 194 ARPGMHSRSKCSVVREAFTDTKLIAISDANELKGFDSQCIEEWDPILFSAAEIWPTKGKP 253 Query: 242 IALVEMDPIDFDFDVDNWDYVTRHLMILKRTPLNTVMDSLGHGGQQYFNSRITDKDLLKK 301 IALVEMDPIDFDFDVDNWDYVTRHLMILKRTPLNTVMDSLGHGGQQYFNSRITDKDLLKK Sbjct: 254 IALVEMDPIDFDFDVDNWDYVTRHLMILKRTPLNTVMDSLGHGGQQYFNSRITDKDLLKK 313 Query: 302 CPIDLTNDEFIYLTKLFMEWPFKPDILMDFVDMYQTEHSG 341 CPIDLTNDEFIYLTKLFMEWPFKPDILMDFVDMYQTEHSG Sbjct: 314 CPIDLTNDEFIYLTKLFMEWPFKPDILMDFVDMYQTEHSG 353 >4GC9-A nrPDB Length = 345 Score = 34.2 bits (77), Expect = 0.082, Method: Composition-based stats. Identities = 18/63 (28%), Positives = 32/63 (50%) Query: 135 VANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLLARPGMHSRSKCSV 194 + N+ S LI++WL I K+ + +G+ KM L A +L+A G S+ S+ Sbjct: 139 IGNLPFSVSTPLIIKWLENISLKDGPFVYGRTKMTLTFQKEVAERLVATTGSKQHSRLSI 198 Query: 195 VRE 197 + + Sbjct: 199 MAQ 201 >1G6H-A nrPDB Length = 257 Score = 31.9 bits (71), Expect = 0.46, Method: Composition-based stats. Identities = 22/85 (25%), Positives = 42/85 (49%), Gaps = 12/85 (14%) Query: 14 LKFFYGFKYLWNPTVYNKIFDKL---DLTKTYKHPEELKVL---------DLYPGVGIQS 61 LK G Y N + NK +L + +T++ P+ LK + ++ PG + Sbjct: 57 LKADEGRVYFENKDITNKEPAELYHYGIVRTFQTPQPLKEMTVLENLLIGEICPGESPLN 116 Query: 62 AIFYNKYCPRQYSLLEKRSSLYKFL 86 ++FY K+ P++ ++EK + +FL Sbjct: 117 SLFYKKWIPKEEEMVEKAFKILEFL 141 >6IRT-A nrPDB Length = 647 Score = 30.4 bits (67), Expect = 1.3, Method: Composition-based stats. Identities = 18/90 (20%), Positives = 40/90 (44%), Gaps = 15/90 (16%) Query: 104 DWSTYSNLIDEERIFVPEVQSSDH--------------INDKFLTVANVTGEGSEGLIMQ 149 +W + E+R+ + SSD + +L+ + TGE ++ L+ Q Sbjct: 377 EWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQ 436 Query: 150 WLSCIGNKNWLYRFGKVKMLL-WMPSTTAR 178 +L+ GN+ + + ++L ++P+ R Sbjct: 437 YLNATGNRWCSWSLSQARLLTSFLPAQLLR 466 >2DH2-A nrPDB Length = 424 Score = 30.4 bits (67), Expect = 1.3, Method: Composition-based stats. Identities = 18/90 (20%), Positives = 40/90 (44%), Gaps = 15/90 (16%) Query: 104 DWSTYSNLIDEERIFVPEVQSSDH--------------INDKFLTVANVTGEGSEGLIMQ 149 +W + E+R+ + SSD + +L+ + TGE ++ L+ Q Sbjct: 156 EWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQ 215 Query: 150 WLSCIGNKNWLYRFGKVKMLL-WMPSTTAR 178 +L+ GN+ + + ++L ++P+ R Sbjct: 216 YLNATGNRWCSWSLSQARLLTSFLPAQLLR 245 >5M99-B nrPDB Length = 505 Score = 28.8 bits (63), Expect = 3.1, Method: Composition-based stats. Identities = 11/31 (35%), Positives = 19/31 (61%) Query: 65 YNKYCPRQYSLLEKRSSLYKFLNAKFEGSPL 95 Y K+ Y ++KR++L+ FL +GSP+ Sbjct: 292 YKKFSESDYEFIKKRAALWYFLIFTLKGSPV 322 >2WWD-A nrPDB Length = 468 Score = 28.8 bits (63), Expect = 3.6, Method: Composition-based stats. Identities = 18/65 (27%), Positives = 28/65 (43%), Gaps = 22/65 (33%) Query: 254 FDVDNWDYVTRHLMILKRTP---------LNTVMDSLGHGGQQ---------YFNSRITD 295 +DV+NW+YV + KR P +N MD++ G Q +R+ Sbjct: 362 YDVENWEYVNKS----KRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH 417 Query: 296 KDLLK 300 D+LK Sbjct: 418 PDILK 422 >6BRH-A nrPDB Length = 672 Score = 28.0 bits (61), Expect = 5.2, Method: Composition-based stats. Identities = 15/43 (34%), Positives = 19/43 (44%), Gaps = 4/43 (9%) Query: 226 PILFSAAEIWPTKGKPIALVEMDPIDFD----FDVDNWDYVTR 264 PI +WP KG+P + I + DVD WDY R Sbjct: 322 PITPVKDSLWPYKGRPATKSFLYEIVSNKRNGIDVDKWDYFAR 364 >4G56-A nrPDB Length = 657 Score = 27.7 bits (60), Expect = 7.5, Method: Composition-based stats. Identities = 28/86 (32%), Positives = 36/86 (41%), Gaps = 11/86 (12%) Query: 248 DPID-FDFDVDNWDYV---TRHLMILKRTPLNTVMDSLGHGGQQYFNSRITDKDLLKKCP 303 DP+ F F N D V R+ + R LNTV+ HG YFN+ + L CP Sbjct: 534 DPLPCFTFHHPNKDDVIDNNRYCCLQYRVDLNTVL----HGFAGYFNTVLYKDVTLSICP 589 Query: 304 IDLTNDEFIYLTKLFMEWPFKPDILM 329 + F + LF P K I M Sbjct: 590 ESHSPGMFSWFPILF---PIKQPIPM 612 >5JXF-A nrPDB Length = 731 Score = 27.7 bits (60), Expect = 8.7, Method: Composition-based stats. Identities = 27/95 (28%), Positives = 39/95 (41%), Gaps = 17/95 (17%) Query: 12 SKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKHPEELKVLDLYPGVGIQSAIFYNKYCPR 71 S+ FF F N TV K+F++L K P+E + L + Y K+ P Sbjct: 450 SQADFFKDF----NSTVDEKVFEQLVALYATKAPKEFLPISLL-------NVEYKKFAPS 498 Query: 72 QYSLLEKRSSLYKFLNAK--FEGSPLQILKRDPYD 104 YS +S L + N K G +LK+ D Sbjct: 499 IYS----KSKLVDYANFKALLSGDAKAVLKKISLD 529 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.322 0.139 0.438 Lambda K H 0.267 0.0445 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,100,943 Number of Sequences: 36641 Number of extensions: 819338 Number of successful extensions: 1612 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 1603 Number of HSP's gapped (non-prelim): 21 length of query: 341 length of database: 10,336,785 effective HSP length: 98 effective length of query: 243 effective length of database: 6,745,967 effective search space: 1639269981 effective search space used: 1639269981 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 60 (27.6 bits)