BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P14902 (403 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6DPQ-A nrPDB 816 0.0 6B9G-B nrPDB 33 0.24 3QOF-A nrPDB 32 0.34 2DKD-A nrPDB 30 1.3 2NWB-A nrPDB 30 1.9 2YCL-A nrPDB 30 2.0 1PHO-A nrPDB 29 4.0 >6DPQ-A nrPDB Length = 425 Score = 816 bits (2108), Expect = 0.0, Method: Compositional matrix adjust. Identities = 392/393 (99%), Positives = 393/393 (100%) Query: 11 ISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL 70 +SKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL Sbjct: 1 MSKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL 60 Query: 71 TDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCV 130 TDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCV Sbjct: 61 TDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCV 120 Query: 131 LANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQM 190 LANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQM Sbjct: 121 LANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQM 180 Query: 191 QERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE 250 QERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE Sbjct: 181 QERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE 240 Query: 251 GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSL 310 GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSL Sbjct: 241 GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSL 300 Query: 311 ESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSED 370 ESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSED Sbjct: 301 ESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSED 360 Query: 371 PSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG 403 PSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG Sbjct: 361 PSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG 393 >6B9G-B nrPDB Length = 340 Score = 33.1 bits (74), Expect = 0.24, Method: Compositional matrix adjust. Identities = 26/112 (23%), Positives = 49/112 (43%), Gaps = 16/112 (14%) Query: 253 WEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLES 312 W P EF+ G+ G + + ++ G+ + LQ++R+++ N C L Sbjct: 220 WSFPYEFSYGADGGAKFLE--------KRLKVSGNQHEELQNVRKHIHSCFTNISCFLLP 271 Query: 313 NPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKE 364 +P ++ D L+E D +K +L+I+ ++L P S KE Sbjct: 272 HPGLKVATNPNFDGKLKEIDDEFIK--------NLKILIPWLLSPESLDIKE 315 >3QOF-A nrPDB Length = 459 Score = 32.3 bits (72), Expect = 0.34, Method: Compositional matrix adjust. Identities = 26/112 (23%), Positives = 49/112 (43%), Gaps = 16/112 (14%) Query: 253 WEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLES 312 W P EF+ G+ G + + ++ G+ + LQ++R+++ N C L Sbjct: 231 WSFPYEFSYGADGGAKFLE--------KRLKVSGNQHEELQNVRKHIHSCFTNISCFLLP 282 Query: 313 NPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKE 364 +P ++ D L+E D +K +L+I+ ++L P S KE Sbjct: 283 HPGLKVATNPNFDGKLKEIDDEFIK--------NLKILIPWLLSPESLDIKE 326 >2DKD-A nrPDB Length = 544 Score = 30.4 bits (67), Expect = 1.3, Method: Compositional matrix adjust. Identities = 21/62 (33%), Positives = 29/62 (46%), Gaps = 6/62 (9%) Query: 310 LESNPSVREFVLSKGDAGLREAYDA------CVKALVSLRSYHLQIVTKYILIPASQQPK 363 L S+P + + G AG R D V + SLRS +LQ T ++I AS P Sbjct: 11 LPSHPKPQGVTFTYGTAGFRMKADKLDYVTFTVGIIASLRSKYLQGKTVGVMITASHNPP 70 Query: 364 EN 365 E+ Sbjct: 71 ED 72 >2NWB-A nrPDB Length = 400 Score = 30.0 bits (66), Expect = 1.9, Method: Compositional matrix adjust. Identities = 35/161 (21%), Positives = 65/161 (40%), Gaps = 24/161 (14%) Query: 206 CLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAG 265 L+ ++ Q+ + ++ FF +R Y ++ +VY G + +FAG + Sbjct: 193 ALQDVIESNQQLFNRLDTDRFFYCVRPYYKPYRVG-----SVVYRG--ANAGDFAGINVI 245 Query: 266 QSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVRE-FVLSKG 324 ++ CF A +Q L D YM P + L P++ + F+ +KG Sbjct: 246 DLTLGLCF---------ANEASYSQMLVDKFLYMMPEDQQILRECMRRPNLMDDFLQAKG 296 Query: 325 -------DAGLREAYDACVKALVSLRSYHLQIVTKYILIPA 358 L+ + C + +H ++VTKYI P+ Sbjct: 297 CIHQDWYQENLKLFIEVCELHGQTAIQHHNELVTKYIAEPS 337 >2YCL-A nrPDB Length = 445 Score = 30.0 bits (66), Expect = 2.0, Method: Compositional matrix adjust. Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 1/55 (1%) Query: 48 DLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKV 102 D + G+++ERVEK+N L D + S L L G A + K R+V Sbjct: 100 DTLSEGEIKERVEKINKLVFDRVGQMHSVNLVALK-GSSQDAATFAKAVATAREV 153 >1PHO-A nrPDB Length = 330 Score = 28.9 bits (63), Expect = 4.0, Method: Compositional matrix adjust. Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Query: 211 LQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSA 264 ++ H + D+ + S +R G+KG Q++D L G WE EFAG A Sbjct: 17 VKAMHYMSDNASKDGDQSYIRF---GFKGETQINDQLTGYGRWE--AEFAGNKA 65 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6DPQ-A nrPDB 835 0.0 Sequences not found previously or not previously below threshold: 6B9G-B nrPDB 33 0.17 3QOF-A nrPDB 33 0.19 2DKD-A nrPDB 32 0.46 1PHO-A nrPDB 30 2.9 4B2Z-A nrPDB 29 4.5 2YCL-A nrPDB 29 4.6 1BWD-A nrPDB 28 6.4 4BKP-A nrPDB 28 8.1 CONVERGED! >6DPQ-A nrPDB Length = 425 Score = 835 bits (2158), Expect = 0.0, Method: Composition-based stats. Identities = 392/393 (99%), Positives = 393/393 (100%) Query: 11 ISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL 70 +SKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL Sbjct: 1 MSKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL 60 Query: 71 TDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCV 130 TDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCV Sbjct: 61 TDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCV 120 Query: 131 LANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQM 190 LANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQM Sbjct: 121 LANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQM 180 Query: 191 QERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE 250 QERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE Sbjct: 181 QERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE 240 Query: 251 GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSL 310 GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSL Sbjct: 241 GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSL 300 Query: 311 ESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSED 370 ESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSED Sbjct: 301 ESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSED 360 Query: 371 PSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG 403 PSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG Sbjct: 361 PSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG 393 >6B9G-B nrPDB Length = 340 Score = 33.4 bits (75), Expect = 0.17, Method: Composition-based stats. Identities = 26/112 (23%), Positives = 49/112 (43%), Gaps = 16/112 (14%) Query: 253 WEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLES 312 W P EF+ G+ G + + ++ G+ + LQ++R+++ N C L Sbjct: 220 WSFPYEFSYGADGGAKFLE--------KRLKVSGNQHEELQNVRKHIHSCFTNISCFLLP 271 Query: 313 NPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKE 364 +P ++ D L+E D +K +L+I+ ++L P S KE Sbjct: 272 HPGLKVATNPNFDGKLKEIDDEFIK--------NLKILIPWLLSPESLDIKE 315 >3QOF-A nrPDB Length = 459 Score = 33.4 bits (75), Expect = 0.19, Method: Composition-based stats. Identities = 26/112 (23%), Positives = 49/112 (43%), Gaps = 16/112 (14%) Query: 253 WEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLES 312 W P EF+ G+ G + + ++ G+ + LQ++R+++ N C L Sbjct: 231 WSFPYEFSYGADGGAKFLE--------KRLKVSGNQHEELQNVRKHIHSCFTNISCFLLP 282 Query: 313 NPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKE 364 +P ++ D L+E D +K +L+I+ ++L P S KE Sbjct: 283 HPGLKVATNPNFDGKLKEIDDEFIK--------NLKILIPWLLSPESLDIKE 326 >2DKD-A nrPDB Length = 544 Score = 31.9 bits (71), Expect = 0.46, Method: Composition-based stats. Identities = 30/88 (34%), Positives = 40/88 (45%), Gaps = 11/88 (12%) Query: 310 LESNPSVREFVLSKGDAGLREAYDA------CVKALVSLRSYHLQIVTKYILIPASQQPK 363 L S+P + + G AG R D V + SLRS +LQ T ++I AS P Sbjct: 11 LPSHPKPQGVTFTYGTAGFRMKADKLDYVTFTVGIIASLRSKYLQGKTVGVMITASHNPP 70 Query: 364 ENKTSE--DP--SKLEAKGTG-GTDLMN 386 E+ + DP S LE+ TDL N Sbjct: 71 EDNGVKVVDPLGSMLESSWEKYATDLAN 98 >1PHO-A nrPDB Length = 330 Score = 29.6 bits (65), Expect = 2.9, Method: Composition-based stats. Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 5/61 (8%) Query: 211 LQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVF 270 ++ H + D+ + S +R G+KG Q++D L G WE EFAG A + Sbjct: 17 VKAMHYMSDNASKDGDQSYIRF---GFKGETQINDQLTGYGRWE--AEFAGNKAESDTAQ 71 Query: 271 Q 271 Q Sbjct: 72 Q 72 >4B2Z-A nrPDB Length = 448 Score = 28.8 bits (63), Expect = 4.5, Method: Composition-based stats. Identities = 22/80 (27%), Positives = 33/80 (41%), Gaps = 14/80 (17%) Query: 193 RDTLLKALLEIASCLEKALQVFH------QIHDHVNP-KAFFSVLRIYLSGWKGNPQLSD 245 R TL +LE S LE+ Q H +P K F V++ YL+GW P+ Sbjct: 66 RITLPTFILEKKSMLERVTNQLQFPEFLLQAHSEKDPLKRFLYVMKWYLAGWHIAPKAVK 125 Query: 246 -------GLVYEGFWEDPKE 258 G + +W+ P + Sbjct: 126 KPLNPVLGEYFTAYWDLPNK 145 >2YCL-A nrPDB Length = 445 Score = 28.8 bits (63), Expect = 4.6, Method: Composition-based stats. Identities = 13/34 (38%), Positives = 19/34 (55%) Query: 48 DLIESGQLRERVEKLNMLSIDHLTDHKSQRLARL 81 D + G+++ERVEK+N L D + S L L Sbjct: 100 DTLSEGEIKERVEKINKLVFDRVGQMHSVNLVAL 133 >1BWD-A nrPDB Length = 348 Score = 28.4 bits (62), Expect = 6.4, Method: Composition-based stats. Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 11/47 (23%) Query: 16 HIDEEV-----GFALPNPQ----ENLPDFYNDWMFIAKHLPDLIESG 53 H+D + G L NP EN+PDF W I P+L++ G Sbjct: 227 HVDSTIVPLRPGLVLTNPSRVNDENMPDFLRSWENIT--CPELVDIG 271 >4BKP-A nrPDB Length = 336 Score = 28.0 bits (61), Expect = 8.1, Method: Composition-based stats. Identities = 11/33 (33%), Positives = 18/33 (54%) Query: 38 DWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL 70 DW+F++ DL ++ Q R EK+ + HL Sbjct: 52 DWVFVSSKDADLTDTAQTRALFEKVQPTHVIHL 84 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.143 0.456 Lambda K H 0.267 0.0439 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 19,175,477 Number of Sequences: 36641 Number of extensions: 884394 Number of successful extensions: 1890 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 1885 Number of HSP's gapped (non-prelim): 20 length of query: 403 length of database: 10,336,785 effective HSP length: 99 effective length of query: 304 effective length of database: 6,709,326 effective search space: 2039635104 effective search space used: 2039635104 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 60 (27.6 bits)