BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P13988 (273 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1F1Z-A nrPDB 565 e-161 3S1A-A nrPDB 30 1.6 6C9C-A nrPDB 29 2.1 5VWM-A nrPDB 29 2.3 5CPT-A nrPDB 28 3.7 1GPU-A nrPDB 28 4.4 5HMP-A nrPDB 28 4.8 5FMZ-A nrPDB 28 5.7 2DBY-A nrPDB 27 8.4 4ZLK-A nrPDB 27 9.2 >1F1Z-A nrPDB Length = 273 Score = 565 bits (1455), Expect = e-161, Method: Compositional matrix adjust. Identities = 273/273 (100%), Positives = 273/273 (100%) Query: 1 MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTGRVHHLLS 60 MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTGRVHHLLS Sbjct: 1 MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTGRVHHLLS 60 Query: 61 DLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDFLVDCK 120 DLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDFLVDCK Sbjct: 61 DLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDFLVDCK 120 Query: 121 DGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWLY 180 DGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWLY Sbjct: 121 DGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWLY 180 Query: 181 SVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAYDLELGKTLSEIRALTANGF 240 SVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAYDLELGKTLSEIRALTANGF Sbjct: 181 SVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAYDLELGKTLSEIRALTANGF 240 Query: 241 IKFNIYKSFRANKCADLCISQVVNMEELRYVAN 273 IKFNIYKSFRANKCADLCISQVVNMEELRYVAN Sbjct: 241 IKFNIYKSFRANKCADLCISQVVNMEELRYVAN 273 >3S1A-A nrPDB Length = 525 Score = 29.6 bits (65), Expect = 1.6, Method: Compositional matrix adjust. Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 5/63 (7%) Query: 150 RRYWQQKQIPWFIFTDKEINPVVKE---NIEWLYSVKTEEVSAELLAQLSPLAHILQEKG 206 R W K I F+ +DK P +K+ N E + S ++ + ++LS + +QEKG Sbjct: 459 RGSWHDKAIREFMISDK--GPDIKDSFRNFERIISGSPTRITVDEKSELSRIVRGVQEKG 516 Query: 207 DEN 209 E+ Sbjct: 517 PES 519 >6C9C-A nrPDB Length = 304 Score = 29.3 bits (64), Expect = 2.1, Method: Compositional matrix adjust. Identities = 15/49 (30%), Positives = 26/49 (53%) Query: 67 FLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDF 115 F+ ++ E SV + ++ +P D +++ + HPV RG Q S DF Sbjct: 129 FIRIKREVSVEEGDKRAVFVPFDGFKVSFEIDFDHPVFRGRTQQASVDF 177 >5VWM-A nrPDB Length = 400 Score = 28.9 bits (63), Expect = 2.3, Method: Compositional matrix adjust. Identities = 15/49 (30%), Positives = 26/49 (53%) Query: 67 FLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDF 115 F+ ++ E SV + ++ +P D +++ + HPV RG Q S DF Sbjct: 225 FIRIKREVSVEEGDKRAVFVPFDGFKVSFEIDFDHPVFRGRTQQASVDF 273 >5CPT-A nrPDB Length = 564 Score = 28.5 bits (62), Expect = 3.7, Method: Compositional matrix adjust. Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 3/70 (4%) Query: 22 GQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTG-RVHHLLSDLELAVFLSLEW-ESSVLDI 79 G+ +Y WL V + P S R + H T R H + DL F ++W S+ + Sbjct: 50 GEDFPSEYEQWLPVPD-PESRRRAGVLLHPTSFRGPHGIGDLGEEAFRFIDWLHSTGCSV 108 Query: 80 REQFPLLPSD 89 + PL+P D Sbjct: 109 WQVLPLVPPD 118 >1GPU-A nrPDB Length = 680 Score = 28.1 bits (61), Expect = 4.4, Method: Compositional matrix adjust. Identities = 35/156 (22%), Positives = 64/156 (41%), Gaps = 33/156 (21%) Query: 101 HPVIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW 160 HPVI V + D + +DGP Q P L R+L +++ W Sbjct: 461 HPVI----WVATHDSIGVGEDGPTHQ------PIETLAHFRSLPNIQV-----------W 499 Query: 161 FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQL--------SPLAHILQEKGDENIIN 212 E++ K ++E ++ +S + L QL S ++LQ+ + +II Sbjct: 500 RPADGNEVSAAYKNSLESKHTPSIIALSRQNLPQLEGSSIESASKGGYVLQDVANPDIIL 559 Query: 213 VCKQVDIAYDLELGKTLS----EIRALTANGFIKFN 244 V +++ +E KTL+ + R ++ F F+ Sbjct: 560 VATGSEVSLSVEAAKTLAAKNIKARVVSLPDFFTFD 595 >5HMP-A nrPDB Length = 750 Score = 27.7 bits (60), Expect = 4.8, Method: Compositional matrix adjust. Identities = 21/76 (27%), Positives = 34/76 (44%), Gaps = 5/76 (6%) Query: 97 SGIKHPVIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQK 156 SG +H I +D F V+ FEQF I LQ + + +LE+ + ++ Sbjct: 420 SGKQHTFIGVLDIYGFETFDVNS----FEQFCINY-ANEKLQQQFNMHVFKLEQEEYMKE 474 Query: 157 QIPWFIFTDKEINPVV 172 IPW + + PV+ Sbjct: 475 DIPWTLIDFYDNQPVI 490 >5FMZ-A nrPDB Length = 751 Score = 27.7 bits (60), Expect = 5.7, Method: Compositional matrix adjust. Identities = 47/212 (22%), Positives = 91/212 (42%), Gaps = 37/212 (17%) Query: 55 VHHLLSDL-----ELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQ 109 V +++SD+ E + +LE + ++R Q+ ++ R IA V R + Q Sbjct: 58 VCYVISDMNFLDEEGKAYTALEGQGKEQNLRPQYEVIEGMPRTIA------WMVQRSLAQ 111 Query: 110 ---VMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQ-----IPWF 161 + + +L D D ++F I+V L D+ +W++K+ + Sbjct: 112 EHGIETPKYLADLFDYKTKRF-IEVGITKGLADDY----------FWKKKEKLGNSMELM 160 Query: 162 IFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAY 221 IF+ + + E S EE +L++L+ L L K ++ + V+ Sbjct: 161 IFSYNQDYSLSNE------SSLDEEGKGRVLSRLTELQAELSLKNLWQVLIGEEDVEKGI 214 Query: 222 DLELGKTLSEIRALTA-NGFIKFNIYKSFRAN 252 D +LG+T+S +R ++ GF F +S+ N Sbjct: 215 DFKLGQTISRLRDISVPAGFSNFEGMRSYIDN 246 >2DBY-A nrPDB Length = 368 Score = 26.9 bits (58), Expect = 8.4, Method: Compositional matrix adjust. Identities = 25/102 (24%), Positives = 52/102 (50%), Gaps = 23/102 (22%) Query: 137 LQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWL-------YSVKTEEVSA 189 L+DER L+R + + +++P PVV ++E++ + K E + Sbjct: 45 LEDERLYA---LQRTFAKGERVP----------PVVPTHVEFVDIAGLVKGAHKGEGLGN 91 Query: 190 ELLA---QLSPLAHILQEKGDENIINVCKQVDIAYDLELGKT 228 + LA +++ +AH+L+ D ++++V +VD D E+ +T Sbjct: 92 QFLAHIREVAAIAHVLRCFPDPDVVHVMGRVDPLEDAEVVET 133 >4ZLK-A nrPDB Length = 839 Score = 26.9 bits (58), Expect = 9.2, Method: Compositional matrix adjust. Identities = 14/49 (28%), Positives = 24/49 (48%), Gaps = 1/49 (2%) Query: 124 FEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVV 172 FEQF I LQ + + +LE+ + ++QIPW + + P + Sbjct: 497 FEQFCINY-ANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCI 544 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1F1Z-A nrPDB 579 e-166 Sequences not found previously or not previously below threshold: 6C9C-A nrPDB 30 1.1 5VWM-A nrPDB 30 1.1 5FMZ-A nrPDB 29 2.1 3S1A-A nrPDB 29 2.2 5CPT-A nrPDB 29 2.2 3ICJ-A nrPDB 28 4.0 2DBY-A nrPDB 28 5.3 1GPU-A nrPDB 27 6.9 CONVERGED! >1F1Z-A nrPDB Length = 273 Score = 579 bits (1493), Expect = e-166, Method: Composition-based stats. Identities = 273/273 (100%), Positives = 273/273 (100%) Query: 1 MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTGRVHHLLS 60 MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTGRVHHLLS Sbjct: 1 MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTGRVHHLLS 60 Query: 61 DLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDFLVDCK 120 DLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDFLVDCK Sbjct: 61 DLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDFLVDCK 120 Query: 121 DGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWLY 180 DGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWLY Sbjct: 121 DGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWLY 180 Query: 181 SVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAYDLELGKTLSEIRALTANGF 240 SVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAYDLELGKTLSEIRALTANGF Sbjct: 181 SVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAYDLELGKTLSEIRALTANGF 240 Query: 241 IKFNIYKSFRANKCADLCISQVVNMEELRYVAN 273 IKFNIYKSFRANKCADLCISQVVNMEELRYVAN Sbjct: 241 IKFNIYKSFRANKCADLCISQVVNMEELRYVAN 273 >6C9C-A nrPDB Length = 304 Score = 30.0 bits (66), Expect = 1.1, Method: Composition-based stats. Identities = 15/49 (30%), Positives = 26/49 (53%) Query: 67 FLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDF 115 F+ ++ E SV + ++ +P D +++ + HPV RG Q S DF Sbjct: 129 FIRIKREVSVEEGDKRAVFVPFDGFKVSFEIDFDHPVFRGRTQQASVDF 177 >5VWM-A nrPDB Length = 400 Score = 30.0 bits (66), Expect = 1.1, Method: Composition-based stats. Identities = 15/49 (30%), Positives = 26/49 (53%) Query: 67 FLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDF 115 F+ ++ E SV + ++ +P D +++ + HPV RG Q S DF Sbjct: 225 FIRIKREVSVEEGDKRAVFVPFDGFKVSFEIDFDHPVFRGRTQQASVDF 273 >5FMZ-A nrPDB Length = 751 Score = 29.3 bits (64), Expect = 2.1, Method: Composition-based stats. Identities = 45/201 (22%), Positives = 85/201 (42%), Gaps = 32/201 (15%) Query: 61 DLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGVDQ---VMSTDFLV 117 D E + +LE + ++R Q+ ++ R IA V R + Q + + +L Sbjct: 69 DEEGKAYTALEGQGKEQNLRPQYEVIEGMPRTIA------WMVQRSLAQEHGIETPKYLA 122 Query: 118 DCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQ-----IPWFIFTDKEINPVV 172 D D ++F I+V L D+ +W++K+ + IF+ + + Sbjct: 123 DLFDYKTKRF-IEVGITKGLADDY----------FWKKKEKLGNSMELMIFSYNQDYSLS 171 Query: 173 KENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAYDLELGKTLSEI 232 E S EE +L++L+ L L K ++ + V+ D +LG+T+S + Sbjct: 172 NE------SSLDEEGKGRVLSRLTELQAELSLKNLWQVLIGEEDVEKGIDFKLGQTISRL 225 Query: 233 RALTA-NGFIKFNIYKSFRAN 252 R ++ GF F +S+ N Sbjct: 226 RDISVPAGFSNFEGMRSYIDN 246 >3S1A-A nrPDB Length = 525 Score = 28.9 bits (63), Expect = 2.2, Method: Composition-based stats. Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 5/63 (7%) Query: 150 RRYWQQKQIPWFIFTDKEINPVVKE---NIEWLYSVKTEEVSAELLAQLSPLAHILQEKG 206 R W K I F+ +DK P +K+ N E + S ++ + ++LS + +QEKG Sbjct: 459 RGSWHDKAIREFMISDK--GPDIKDSFRNFERIISGSPTRITVDEKSELSRIVRGVQEKG 516 Query: 207 DEN 209 E+ Sbjct: 517 PES 519 >5CPT-A nrPDB Length = 564 Score = 28.9 bits (63), Expect = 2.2, Method: Composition-based stats. Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 3/70 (4%) Query: 22 GQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTG-RVHHLLSDLELAVFLSLEW-ESSVLDI 79 G+ +Y WL V + P S R + H T R H + DL F ++W S+ + Sbjct: 50 GEDFPSEYEQWLPVPD-PESRRRAGVLLHPTSFRGPHGIGDLGEEAFRFIDWLHSTGCSV 108 Query: 80 REQFPLLPSD 89 + PL+P D Sbjct: 109 WQVLPLVPPD 118 >3ICJ-A nrPDB Length = 534 Score = 28.1 bits (61), Expect = 4.0, Method: Composition-based stats. Identities = 24/74 (32%), Positives = 39/74 (52%), Gaps = 6/74 (8%) Query: 114 DFLVDCKDGPFEQ--FAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEI-NP 170 D VD FE+ F+ +++ A+ ++D++ EL+ R Q P FI +D I N Sbjct: 352 DKAVDVALDAFEEAEFSGRIEHASLVRDDQLERIKELKVRISAQ---PHFIVSDWWIVNR 408 Query: 171 VVKENIEWLYSVKT 184 V +E +W Y +KT Sbjct: 409 VGEERAKWAYRLKT 422 >2DBY-A nrPDB Length = 368 Score = 27.7 bits (60), Expect = 5.3, Method: Composition-based stats. Identities = 26/106 (24%), Positives = 51/106 (48%), Gaps = 14/106 (13%) Query: 133 PAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWL-------YSVKTE 185 P A + + LE ER Y Q+ + + PVV ++E++ + K E Sbjct: 32 PFATIDKNVGVVPLEDERLYALQRTFA----KGERVPPVVPTHVEFVDIAGLVKGAHKGE 87 Query: 186 EVSAELLA---QLSPLAHILQEKGDENIINVCKQVDIAYDLELGKT 228 + + LA +++ +AH+L+ D ++++V +VD D E+ +T Sbjct: 88 GLGNQFLAHIREVAAIAHVLRCFPDPDVVHVMGRVDPLEDAEVVET 133 >1GPU-A nrPDB Length = 680 Score = 27.3 bits (59), Expect = 6.9, Method: Composition-based stats. Identities = 35/156 (22%), Positives = 64/156 (41%), Gaps = 33/156 (21%) Query: 101 HPVIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW 160 HPVI V + D + +DGP Q P L R+L +++ W Sbjct: 461 HPVI----WVATHDSIGVGEDGPTHQ------PIETLAHFRSLPNIQV-----------W 499 Query: 161 FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQL--------SPLAHILQEKGDENIIN 212 E++ K ++E ++ +S + L QL S ++LQ+ + +II Sbjct: 500 RPADGNEVSAAYKNSLESKHTPSIIALSRQNLPQLEGSSIESASKGGYVLQDVANPDIIL 559 Query: 213 VCKQVDIAYDLELGKTLS----EIRALTANGFIKFN 244 V +++ +E KTL+ + R ++ F F+ Sbjct: 560 VATGSEVSLSVEAAKTLAAKNIKARVVSLPDFFTFD 595 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.135 0.395 Lambda K H 0.267 0.0415 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,113,939 Number of Sequences: 36641 Number of extensions: 457885 Number of successful extensions: 1131 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 1123 Number of HSP's gapped (non-prelim): 25 length of query: 273 length of database: 10,336,785 effective HSP length: 96 effective length of query: 177 effective length of database: 6,819,249 effective search space: 1207007073 effective search space used: 1207007073 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 58 (26.9 bits)