BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P13516 (355 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4YMK-A nrPDB 697 0.0 4ZYO-A nrPDB 588 e-168 2ZJG-A nrPDB 35 0.041 2A5H-A nrPDB 29 3.5 >4YMK-A nrPDB Length = 339 Score = 697 bits (1799), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/332 (100%), Positives = 332/332 (100%) Query: 24 SGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGL 83 SGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGL Sbjct: 2 SGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGL 61 Query: 84 YGIILVPSCKLYTCLFGIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQ 143 YGIILVPSCKLYTCLFGIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQ Sbjct: 62 YGIILVPSCKLYTCLFGIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQ 121 Query: 144 NDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKA 203 NDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKA Sbjct: 122 NDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKA 181 Query: 204 EKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSA 263 EKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSA Sbjct: 182 EKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSA 241 Query: 264 AHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCM 323 AHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCM Sbjct: 242 AHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCM 301 Query: 324 AALGLAYDRKKVSKATVLARIKRTGDGSHKSS 355 AALGLAYDRKKVSKATVLARIKRTGDGSHKSS Sbjct: 302 AALGLAYDRKKVSKATVLARIKRTGDGSHKSS 333 >4ZYO-A nrPDB Length = 318 Score = 588 bits (1515), Expect = e-168, Method: Compositional matrix adjust. Identities = 272/316 (86%), Positives = 295/316 (93%) Query: 40 EDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGLYGIILVPSCKLYTCLF 99 DIRP++K+DI+DPTY D GP PK+EYVWRNIILM LLHLG LYGI L+P+CK YT L+ Sbjct: 3 SDIRPDIKDDIYDPTYADAAGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLW 62 Query: 100 GIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQNDVYEWARDHRAHHKF 159 G+FYY SALGITAGAHRLWSHR+YKARLPLR+FLIIANTMAFQNDVYEWARDHRAHHKF Sbjct: 63 GVFYYFVSALGITAGAHRLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKF 122 Query: 160 SETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKAEKLVMFQRRYYKPGLL 219 SETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKG LD+SDL+AEKLVMFQRRYYKPGLL Sbjct: 123 SETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLL 182 Query: 220 LMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSAAHLYGYRPYDKNIQSR 279 +MCFILPTLVPWY WGETF NS+FV+TFLRY +VLNATWLVNSAAHL+GYRPYDKNI R Sbjct: 183 MMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPR 242 Query: 280 ENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAT 339 ENILVSLGAVGEGFHNYHH+FP+DYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKA Sbjct: 243 ENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAA 302 Query: 340 VLARIKRTGDGSHKSS 355 +LARIKRTGDG++KS Sbjct: 303 ILARIKRTGDGNYKSG 318 >2ZJG-A nrPDB Length = 410 Score = 35.4 bits (80), Expect = 0.041, Method: Compositional matrix adjust. Identities = 25/97 (25%), Positives = 42/97 (43%), Gaps = 1/97 (1%) Query: 201 LKAEKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLV 260 L + V F + P ++ + P + P E + F+ +YT LV Sbjct: 8 LDSNVWVEFTKLAADPSVVNLGQGFPDISPPSYVKEELSKAAFIDNMNQYTRGFGHPALV 67 Query: 261 NSAAHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYH 297 + + LYG + Y + I E ILV++GA G F++ Sbjct: 68 KALSCLYG-KIYQRQIDPNEEILVAVGAYGSLFNSIQ 103 >2A5H-A nrPDB Length = 416 Score = 28.9 bits (63), Expect = 3.5, Method: Compositional matrix adjust. Identities = 14/67 (20%), Positives = 35/67 (52%), Gaps = 3/67 (4%) Query: 18 TITAPPSGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVL 77 ++ P N+ + + +P LE + + D+ DP ++D + P P L + + + +L+++ Sbjct: 65 SLIDPNDPNDPVRKQAIPTALELN---KAAADLEDPLHEDTDSPVPGLTHRYPDRVLLLI 121 Query: 78 LHLGGLY 84 + +Y Sbjct: 122 TDMCSMY 128 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4YMK-A nrPDB 663 0.0 4ZYO-A nrPDB 625 e-179 Sequences not found previously or not previously below threshold: 2ZJG-A nrPDB 36 0.026 2A5H-A nrPDB 29 3.9 3C8Z-A nrPDB 28 8.4 CONVERGED! >4YMK-A nrPDB Length = 339 Score = 663 bits (1711), Expect = 0.0, Method: Composition-based stats. Identities = 332/332 (100%), Positives = 332/332 (100%) Query: 24 SGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGL 83 SGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGL Sbjct: 2 SGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGL 61 Query: 84 YGIILVPSCKLYTCLFGIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQ 143 YGIILVPSCKLYTCLFGIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQ Sbjct: 62 YGIILVPSCKLYTCLFGIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQ 121 Query: 144 NDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKA 203 NDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKA Sbjct: 122 NDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKA 181 Query: 204 EKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSA 263 EKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSA Sbjct: 182 EKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSA 241 Query: 264 AHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCM 323 AHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCM Sbjct: 242 AHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCM 301 Query: 324 AALGLAYDRKKVSKATVLARIKRTGDGSHKSS 355 AALGLAYDRKKVSKATVLARIKRTGDGSHKSS Sbjct: 302 AALGLAYDRKKVSKATVLARIKRTGDGSHKSS 333 >4ZYO-A nrPDB Length = 318 Score = 625 bits (1611), Expect = e-179, Method: Composition-based stats. Identities = 272/316 (86%), Positives = 295/316 (93%) Query: 40 EDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGLYGIILVPSCKLYTCLF 99 DIRP++K+DI+DPTY D GP PK+EYVWRNIILM LLHLG LYGI L+P+CK YT L+ Sbjct: 3 SDIRPDIKDDIYDPTYADAAGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLW 62 Query: 100 GIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQNDVYEWARDHRAHHKF 159 G+FYY SALGITAGAHRLWSHR+YKARLPLR+FLIIANTMAFQNDVYEWARDHRAHHKF Sbjct: 63 GVFYYFVSALGITAGAHRLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKF 122 Query: 160 SETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKAEKLVMFQRRYYKPGLL 219 SETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKG LD+SDL+AEKLVMFQRRYYKPGLL Sbjct: 123 SETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLL 182 Query: 220 LMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSAAHLYGYRPYDKNIQSR 279 +MCFILPTLVPWY WGETF NS+FV+TFLRY +VLNATWLVNSAAHL+GYRPYDKNI R Sbjct: 183 MMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPR 242 Query: 280 ENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAT 339 ENILVSLGAVGEGFHNYHH+FP+DYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKA Sbjct: 243 ENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAA 302 Query: 340 VLARIKRTGDGSHKSS 355 +LARIKRTGDG++KS Sbjct: 303 ILARIKRTGDGNYKSG 318 >2ZJG-A nrPDB Length = 410 Score = 36.1 bits (82), Expect = 0.026, Method: Composition-based stats. Identities = 25/95 (26%), Positives = 42/95 (44%), Gaps = 1/95 (1%) Query: 201 LKAEKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLV 260 L + V F + P ++ + P + P E + F+ +YT LV Sbjct: 8 LDSNVWVEFTKLAADPSVVNLGQGFPDISPPSYVKEELSKAAFIDNMNQYTRGFGHPALV 67 Query: 261 NSAAHLYGYRPYDKNIQSRENILVSLGAVGEGFHN 295 + + LYG + Y + I E ILV++GA G F++ Sbjct: 68 KALSCLYG-KIYQRQIDPNEEILVAVGAYGSLFNS 101 >2A5H-A nrPDB Length = 416 Score = 28.8 bits (63), Expect = 3.9, Method: Composition-based stats. Identities = 14/67 (20%), Positives = 35/67 (52%), Gaps = 3/67 (4%) Query: 18 TITAPPSGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVL 77 ++ P N+ + + +P LE + + D+ DP ++D + P P L + + + +L+++ Sbjct: 65 SLIDPNDPNDPVRKQAIPTALELN---KAAADLEDPLHEDTDSPVPGLTHRYPDRVLLLI 121 Query: 78 LHLGGLY 84 + +Y Sbjct: 122 TDMCSMY 128 >3C8Z-A nrPDB Length = 414 Score = 27.6 bits (60), Expect = 8.4, Method: Composition-based stats. Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Query: 156 HHKFSETHADPHNSRRGF--FFSHVGWLLVRKHPAVKEKGGKLDMSDLKAE 204 HH++S HA+ R F + H G + H K +G + +S L+A+ Sbjct: 258 HHEYSAAHAESVTGERRFARHYVHTGMIGWDGHKMSKSRGNLVLVSQLRAQ 308 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.144 0.472 Lambda K H 0.267 0.0438 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,679,718 Number of Sequences: 36641 Number of extensions: 854839 Number of successful extensions: 2475 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 2461 Number of HSP's gapped (non-prelim): 22 length of query: 355 length of database: 10,336,785 effective HSP length: 98 effective length of query: 257 effective length of database: 6,745,967 effective search space: 1733713519 effective search space used: 1733713519 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.3 bits) S2: 60 (27.6 bits)