BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P13232
         (177 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

3DI2-A nrPDB                                                          309   4e-85
4ODI-A nrPDB                                                           31   0.28 
5IKP-A nrPDB                                                           29   1.1  
3V7C-A nrPDB                                                           27   3.7  
5FV7-A nrPDB                                                           27   4.6  
4QQW-A nrPDB                                                           27   6.7  
5FM1-C nrPDB                                                           27   6.8  
4LNN-A nrPDB                                                           26   9.1  

>3DI2-A nrPDB
          Length = 154

 Score =  309 bits (792), Expect = 4e-85,   Method: Compositional matrix adjust.
 Identities = 151/153 (98%), Positives = 151/153 (98%)

Query: 25  SDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRA 84
            DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRA
Sbjct: 2   GDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRA 61

Query: 85  ARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSL 144
           ARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALG AQPTKSLEENKSL
Sbjct: 62  ARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGAAQPTKSLEENKSL 121

Query: 145 KEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH 177
           KEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH
Sbjct: 122 KEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH 154


>4ODI-A nrPDB
          Length = 281

 Score = 30.8 bits (68), Expect = 0.28,   Method: Compositional matrix adjust.
 Identities = 23/90 (25%), Positives = 39/90 (43%), Gaps = 4/90 (4%)

Query: 85  ARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSL 144
           A   R+F    +   + L L++  E T    N   +  G     L      +++E  K+L
Sbjct: 4   ASTFRRFFGNMAKAKYTLVLIRHGEST---WNKENRFTGWTDVPLSPVGEQEAVEAAKAL 60

Query: 145 KEQKKLNDLCFLKRLLQEIKTCWNKILMGT 174
           KE+    D+ +   L + + TCW  +L GT
Sbjct: 61  KEKGFEFDVAYTSVLQRAVVTCWT-VLKGT 89


>5IKP-A nrPDB
          Length = 846

 Score = 28.9 bits (63), Expect = 1.1,   Method: Compositional matrix adjust.
 Identities = 15/51 (29%), Positives = 25/51 (49%)

Query: 67  FKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNC 117
           F R +    +E    F A  +    +K+N +  FD+H+ ++ E    LLNC
Sbjct: 534 FIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNC 584


>3V7C-A nrPDB
          Length = 329

 Score = 27.3 bits (59), Expect = 3.7,   Method: Compositional matrix adjust.
 Identities = 13/28 (46%), Positives = 20/28 (71%)

Query: 144 LKEQKKLNDLCFLKRLLQEIKTCWNKIL 171
           L ++ KL+   FL+RLLQEI+  +N+ L
Sbjct: 233 LHDKNKLDRYQFLERLLQEIEKRYNQFL 260


>5FV7-A nrPDB
          Length = 353

 Score = 26.9 bits (58), Expect = 4.6,   Method: Compositional matrix adjust.
 Identities = 25/85 (29%), Positives = 40/85 (47%), Gaps = 7/85 (8%)

Query: 9  IFGLPPLILVLLPVASSDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFK 68
          I GL  LI  + P A  + DI+   G++      +SI Q L ++++ G + L NE     
Sbjct: 3  IQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQ-GGDVLQNEEGETT 61

Query: 69 RHICDANKEGMFLFRAARKLRQFLK 93
           H+      GMF +R  R +   +K
Sbjct: 62 SHLM-----GMF-YRTIRMMENGIK 80


>4QQW-A nrPDB
          Length = 964

 Score = 26.6 bits (57), Expect = 6.7,   Method: Composition-based stats.
 Identities = 15/55 (27%), Positives = 24/55 (43%)

Query: 119 GQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMG 173
           G   GR+P        T+ +E++  L     ++DL  +  LLQ    CW    +G
Sbjct: 663 GAQSGRRPTRGAVLVATQVVEQSLDLDVDLMISDLAPVSLLLQRAGRCWRHEHLG 717


>5FM1-C nrPDB
          Length = 473

 Score = 26.6 bits (57), Expect = 6.8,   Method: Compositional matrix adjust.
 Identities = 22/77 (28%), Positives = 32/77 (41%), Gaps = 5/77 (6%)

Query: 28  DIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARK 87
           DI  K    Y+ +L      LLD    + S  +NN   F   +    N E   + RA  K
Sbjct: 283 DIAHKGHSSYDVML-----DLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTK 337

Query: 88  LRQFLKMNSTGDFDLHL 104
           L+Q +K  S     +H+
Sbjct: 338 LQQRIKFPSWSSSAMHV 354


>4LNN-A nrPDB
          Length = 443

 Score = 25.8 bits (55), Expect = 9.1,   Method: Compositional matrix adjust.
 Identities = 24/89 (26%), Positives = 39/89 (43%), Gaps = 12/89 (13%)

Query: 22  VASSDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFL 81
           VA   CDI   DG  +E     ++ ++L  M+++G     ++FN          +   FL
Sbjct: 88  VARFICDIYNPDGTPFEGDPRNNLKRILKEMEDLGF----SDFNL-------GPEPEFFL 136

Query: 82  FRAARKLRQFLKMNSTGD-FDLHLLKVSE 109
           F+   K    L++N  G  FDL    + E
Sbjct: 137 FKLDEKGEPTLELNDKGGYFDLAPTDLGE 165


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

3DI2-A nrPDB                                                          293   3e-80

Sequences not found previously or not previously below threshold:

5IKP-A nrPDB                                                           30   0.74 
4ODI-A nrPDB                                                           30   0.79 
3V7C-A nrPDB                                                           28   2.9  
4QQW-A nrPDB                                                           26   6.1  
4LNN-A nrPDB                                                           26   7.0  
1CYG-A nrPDB                                                           26   7.0  


CONVERGED!
>3DI2-A nrPDB
          Length = 154

 Score =  293 bits (750), Expect = 3e-80,   Method: Composition-based stats.
 Identities = 151/153 (98%), Positives = 151/153 (98%)

Query: 25  SDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRA 84
            DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRA
Sbjct: 2   GDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRA 61

Query: 85  ARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSL 144
           ARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALG AQPTKSLEENKSL
Sbjct: 62  ARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGAAQPTKSLEENKSL 121

Query: 145 KEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH 177
           KEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH
Sbjct: 122 KEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH 154


>5IKP-A nrPDB
          Length = 846

 Score = 29.5 bits (65), Expect = 0.74,   Method: Composition-based stats.
 Identities = 15/51 (29%), Positives = 25/51 (49%)

Query: 67  FKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNC 117
           F R +    +E    F A  +    +K+N +  FD+H+ ++ E    LLNC
Sbjct: 534 FIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNC 584


>4ODI-A nrPDB
          Length = 281

 Score = 29.5 bits (65), Expect = 0.79,   Method: Composition-based stats.
 Identities = 23/90 (25%), Positives = 39/90 (43%), Gaps = 4/90 (4%)

Query: 85  ARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSL 144
           A   R+F    +   + L L++  E T    N   +  G     L      +++E  K+L
Sbjct: 4   ASTFRRFFGNMAKAKYTLVLIRHGEST---WNKENRFTGWTDVPLSPVGEQEAVEAAKAL 60

Query: 145 KEQKKLNDLCFLKRLLQEIKTCWNKILMGT 174
           KE+    D+ +   L + + TCW  +L GT
Sbjct: 61  KEKGFEFDVAYTSVLQRAVVTCWT-VLKGT 89


>3V7C-A nrPDB
          Length = 329

 Score = 27.6 bits (60), Expect = 2.9,   Method: Composition-based stats.
 Identities = 13/28 (46%), Positives = 20/28 (71%)

Query: 144 LKEQKKLNDLCFLKRLLQEIKTCWNKIL 171
           L ++ KL+   FL+RLLQEI+  +N+ L
Sbjct: 233 LHDKNKLDRYQFLERLLQEIEKRYNQFL 260


>4QQW-A nrPDB
          Length = 964

 Score = 26.4 bits (57), Expect = 6.1,   Method: Composition-based stats.
 Identities = 15/55 (27%), Positives = 24/55 (43%)

Query: 119 GQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMG 173
           G   GR+P        T+ +E++  L     ++DL  +  LLQ    CW    +G
Sbjct: 663 GAQSGRRPTRGAVLVATQVVEQSLDLDVDLMISDLAPVSLLLQRAGRCWRHEHLG 717


>4LNN-A nrPDB
          Length = 443

 Score = 26.4 bits (57), Expect = 7.0,   Method: Composition-based stats.
 Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 12/82 (14%)

Query: 22  VASSDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFL 81
           VA   CDI   DG  +E     ++ ++L  M+++G     ++FN          +   FL
Sbjct: 88  VARFICDIYNPDGTPFEGDPRNNLKRILKEMEDLGF----SDFNL-------GPEPEFFL 136

Query: 82  FRAARKLRQFLKMNSTGD-FDL 102
           F+   K    L++N  G  FDL
Sbjct: 137 FKLDEKGEPTLELNDKGGYFDL 158


>1CYG-A nrPDB
          Length = 680

 Score = 26.4 bits (57), Expect = 7.0,   Method: Composition-based stats.
 Identities = 14/25 (56%), Positives = 17/25 (68%), Gaps = 2/25 (8%)

Query: 74  ANKEGMFL--FRAARKLRQFLKMNS 96
           AN+ GM L  FR  +KLRQ L+ NS
Sbjct: 269 ANESGMSLLDFRFGQKLRQVLRNNS 293


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.322    0.146    0.449 

Lambda     K      H
   0.267   0.0445    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,042,077
Number of Sequences: 36641
Number of extensions: 352584
Number of successful extensions: 760
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 749
Number of HSP's gapped (non-prelim): 23
length of query: 177
length of database: 10,336,785
effective HSP length: 91
effective length of query: 86
effective length of database: 7,002,454
effective search space: 602211044
effective search space used: 602211044
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.1 bits)